scholarly journals Regulation of the Later Stages of Nodulation Stimulated by IPD3/CYCLOPS Transcription Factor and Cytokinin in Pea Pisum sativum L.

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 56
Author(s):  
Elizaveta S. Rudaya ◽  
Polina Yu. Kozyulina ◽  
Olga A. Pavlova ◽  
Alexandra V. Dolgikh ◽  
Alexandra N. Ivanova ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Comparative Analysis ◽  
Pisum Sativum ◽  
Nodule Development ◽  
Rna Seq ◽  
Wild Type ◽  
Development Stages ◽  
Pisum Sativum L ◽  
Pea Mutant

The IPD3/CYCLOPS transcription factor was shown to be involved in the regulation of nodule primordia development and subsequent stages of nodule differentiation. In contrast to early stages, the stages related to nodule differentiation remain less studied. Recently, we have shown that the accumulation of cytokinin at later stages may significantly impact nodule development. This conclusion was based on a comparative analysis of cytokinin localization between pea wild type and ipd3/cyclops mutants. However, the role of cytokinin at these later stages of nodulation is still far from understood. To determine a set of genes involved in the regulation of later stages of nodule development connected with infection progress, intracellular accommodation, as well as plant tissue and bacteroid differentiation, the RNA-seq analysis of pea mutant SGEFix--2 (sym33) nodules impaired in these processes compared to wild type SGE nodules was performed. To verify cytokinin’s influence on late nodule development stages, the comparative RNA-seq analysis of SGEFix--2 (sym33) mutant plants treated with cytokinin was also conducted. Findings suggest a significant role of cytokinin in the regulation of later stages of nodule development.

scholarly journals Organization of the endoplasmic reticulum in cells of effective and ineffective pea nodules (Pisum sativum L.)

2019 ◽  
Vol 17 (4) ◽  
pp. 5-14
Author(s):  
Anna V. Tsyganova ◽  
Viktor E. Tsyganov
Keyword(s):  
Endoplasmic Reticulum ◽  
Pisum Sativum ◽  
Structural Changes ◽  
Nodule Development ◽  
Wild Type ◽  
Meristematic Cells ◽  
Pisum Sativum L ◽  
Transmission Electron ◽  
Infected Cells ◽  
Membrane Bound

Background. The endoplasmic reticulum (ER) is the largest membrane-bound organelle, which plays an important role in the functioning of a plant cell and participates in its differentiation. Materials and methods. Using the methods of transmission electron microscopy, the morphological features and dynamics of structural changes in the ER in symbiotic nodules of pea (Pisum sativum L.) wild-type and mutants blocked at different stages of nodule development were studied. Results. ER developed from a network of individual tubules in meristematic cells, to a developed network of cisterns around the nucleus and plasmalemma, and a network of granular and smooth tubules accompanying infection structures in colonized and infected cells and symbiosomes in infected cells. Conclusions. A correlation was found between the level of development of the ER network and the degree of bacteroid differentiation.

scholarly journals Laser Microdissection of Pisum sativum L. Nodules Followed by RNA-Seq Analysis Revealed Crucial Transcriptomic Changes During Infected Cell Differentiation

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2504
Author(s):  
Pyotr G. Kusakin ◽  
Tatiana A. Serova ◽  
Natalia E. Gogoleva ◽  
Yuri V. Gogolev ◽  
Viktor E. Tsyganov
Keyword(s):  
Nitrogen Fixation ◽  
Pisum Sativum ◽  
Laser Microdissection ◽  
Host Cells ◽  
Nodule Development ◽  
Nodule Formation ◽  
Rna Seq ◽  
Legume Crop ◽  
Pisum Sativum L ◽  
Transcriptional Changes

Garden pea (Pisum sativum L.) is a globally important legume crop. Like other legumes, it forms beneficial symbiotic interactions with the soil bacteria rhizobia, gaining the ability to fix atmospheric nitrogen. In pea nodules, the meristem is long-lasting and results in the formation of several histological zones that implicate a notable differentiation of infected host cells. However, the fine transcriptional changes that accompany differentiation are still unknown. In this study, using laser microdissection followed by RNA-seq analysis, we performed transcriptomic profiling in the early infection zone, late infection zone, and nitrogen fixation zone of 11-day-old nodules of pea wild-type line SGE. As a result, a list of functional groups of differentially expressed genes (DEGs) in different nodule histological zones and a list of genes with the most prominent expression changes during nodule development were obtained. Their analyses demonstrated that the highest amount of DEGs was associated with the nitrogen fixation zone. Among well-known genes controlling nodule development, we revealed genes that can be novel players throughout nodule formation. The characterized genes in pea were compared with those previously described in other legumes and their possible functions in nodule development are discussed.

scholarly journals Identification of Pisum sativum L. cytokinin and auxin metabolic and signaling genes, and an analysis of their role in symbiotic nodule development

2017 ◽  
Vol 9 (3) ◽  
pp. 22-35 ◽  
Author(s):  
E. A. Dolgikh ◽  
A. I. Shaposhnikov ◽  
A. V. Dolgikh ◽  
E. S. Gribchenko ◽  
K. B. Bodyagina ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Nodule Development ◽  
Pisum Sativum L ◽  
Symbiotic Nodule

scholarly journals Effects of mixed saline and alkaline stress on the morphology and anatomy of Pisum sativum L.: The role of peroxidase and ascorbate oxidase in growth regulation

2013 ◽  
Vol 65 (1) ◽  
pp. 265-278 ◽  
Author(s):  
Kukavica Biljana ◽  
Morina Filis ◽  
Janjic Nina ◽  
Boroja Mirela ◽  
Jovanovic Lj. ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Growth Regulation ◽  
Ascorbate Oxidase ◽  
Alkaline Stress ◽  
Pisum Sativum L

scholarly journals The role of the NMD factor UPF3B in olfactory sensory neurons

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Kun Tan ◽  
Samantha H Jones ◽  
Blue B Lake ◽  
Jennifer N Dumdie ◽  
Eleen Y Shum ◽  
...  
Keyword(s):  
Human Cognition ◽  
Cell Populations ◽  
Rna Seq ◽  
Wild Type ◽  
Single Cell Rna Sequencing ◽  
Nonsense Mediated Rna Decay ◽  
Different Levels ◽  
Selection Of

The UPF3B-dependent branch of the nonsense-mediated RNA decay (NMD) pathway is critical for human cognition. Here, we examined the role of UPF3B in the olfactory system. Single-cell RNA-sequencing (scRNA-seq) analysis demonstrated considerable heterogeneity of olfactory sensory neuron (OSN) cell populations in wild-type (WT) mice, and revealed that UPF3B loss influences specific subsets of these cell populations. UPF3B also regulates the expression of a large cadre of antimicrobial genes in OSNs, and promotes the selection of specific olfactory receptor (Olfr) genes for expression in mature OSNs (mOSNs). RNA-seq and Ribotag analyses identified classes of mRNAs expressed and translated at different levels in WT and Upf3b-null mOSNs. Integrating multiple computational approaches, UPF3B-dependent NMD target transcripts that are candidates to mediate the functions of NMD in mOSNs were identified in vivo. Together, our data provides a valuable resource for the olfactory field and insights into the roles of NMD in vivo.

scholarly journals Growth, seed development and genetic analysis in wild type and Def mutant of Pisum sativum L

2011 ◽  
Vol 4 (1) ◽  
pp. 489 ◽  
Author(s):  
Kwadwo Ayeh ◽  
YeonKyeong Lee ◽  
Mike J Ambrose ◽  
Anne Hvoslef-Eide
Keyword(s):  
Pisum Sativum ◽  
Genetic Analysis ◽  
Seed Development ◽  
Wild Type ◽  
Pisum Sativum L

scholarly journals Association of intronic DNA methylation and hydroxymethylation alterations in the epigenetic etiology of dilated cardiomyopathy

2019 ◽  
Vol 317 (1) ◽  
pp. H168-H180 ◽  
Author(s):  
Ali M. Tabish ◽  
Mohammed Arif ◽  
Taejeong Song ◽  
Zaher Elbeck ◽  
Richard C. Becker ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Dilated Cardiomyopathy ◽  
Cardiac Remodeling ◽  
Rna Seq ◽  
Wild Type ◽  
Kegg Pathways ◽  
Wild Type Phenotype ◽  
Gene Expression Levels

In this study, we investigated the role of DNA methylation [5-methylcytosine (5mC)] and 5-hydroxymethylcytosine (5hmC), epigenetic modifications that regulate gene activity, in dilated cardiomyopathy (DCM). A MYBPC3 mutant mouse model of DCM was compared with wild type and used to profile genomic 5mC and 5hmC changes by Chip-seq, and gene expression levels were analyzed by RNA-seq. Both 5mC-altered genes (957) and 5hmC-altered genes (2,022) were identified in DCM hearts. Diverse gene ontology and KEGG pathways were enriched for DCM phenotypes, such as inflammation, tissue fibrosis, cell death, cardiac remodeling, cardiomyocyte growth, and differentiation, as well as sarcomere structure. Hierarchical clustering of mapped genes affected by 5mC and 5hmC clearly differentiated DCM from wild-type phenotype. Based on these data, we propose that genomewide 5mC and 5hmC contents may play a major role in DCM pathogenesis. NEW & NOTEWORTHY Our data demonstrate that development of dilated cardiomyopathy in mice is associated with significant epigenetic changes, specifically in intronic regions, which, when combined with gene expression profiling data, highlight key signaling pathways involved in pathological cardiac remodeling and heart contractile dysfunction.

THE ROLE OF ETHYLENE IN THE RESPONSES OF PEAS (PISUM SATIVUM L.) TO SALINE STRESS

1993 ◽  
pp. 175-182
Author(s):  
S.O. El-Abd ◽  
A.F. Abou-Hadid ◽  
A.R. Smith ◽  
M.A. Hall
Keyword(s):  
Pisum Sativum ◽  
Saline Stress ◽  
Pisum Sativum L
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