Evaluation of the content and the pathogenetic role of cytokines in the peritoneal fluid in patients with deep infiltrative endometriosis

Background. Deep infiltrative endometriosis (DIE) is characterized by the invasion of endometriosis lesions in tissues and organs to a depth of over 5 mm. In recent years, the proportion of infiltrative forms of endometriosis has been steadily increasing. The main clinical manifestations is chronic pelvic pain syndrome and infertility. A key element in the pathogenesis of deep infiltrative endometriosis is an ineffective inflammatory response.Objective. Evaluate the content and the role of pro- and anti-inflammatory cytokines, growth factors and chemokines in the pathogenesis of deep infiltrative endometriosis for pathogenetically grounded immunomodulatory therapy.Materials and methods. The present study included 120 women with deep infiltrative endometriosis. In the peritoneal fluid, using IFA determined the level of IL-33, and with the help of running cytofluometry format NEA has estimated the levels of IL-2, IL-6, IL-10, IP-10, MCP-1 and growth factors – FGF, TGF-β.Results. In the study of peritoneal fluid of patients with DIE was a significant decrease in the level of IL-2 and IL-10 6.7 times compared to the control group. The level of IL-6 was increased in 1.5 times, as well as the level of IL-33, and was awarded the data link cytokines with the severity of pain. DIE is characterized by increased levels of MCP-1 in 2 times and decrease in IP-10 1.3 times, as well as increased levels of FGF 1.5 times and reduced levels of TGF-β in 1.9 times in comparison with the control group.Conclusion. For effective treatment of DIE and to increase the duration of recurrence-free period actual and pathogenetically justified is the inclusion of a combined treatment of immunomodulatory therapy with recombinant IL-2 aimed at the elimination of immunological disorders in the pelvic cavity.

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Concentrations of Neopterin and Interleukin-10 in Peritoneal Fluid and in Serum of Women with Endometriosis

Pteridines ◽

10.1515/pteridines.2004.15.1.20 ◽

2004 ◽

Vol 15 (1) ◽

pp. 20-27 ◽

Cited By ~ 1

Author(s):

Zdzislawa Kondera-Anasz ◽

Justyna Sikora ◽

Anna Mertas ◽

Piotr Miciñski ◽

Bartlomiej Bednarz

Keyword(s):

Growth Factors ◽

Peritoneal Fluid ◽

Interleukin 10 ◽

Peritoneal Macrophages ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Serum Concentrations ◽

Macrophage Activity ◽

The Mean

Abstract Endometriosis is a gynaecological disorder characterized by increased number and activation of peritoneal macrophages and release of macrophage-derived cytokines and growth factors. The aim of our work was t(5 study the level of neopterin and interleukin (TL)-10 in peritoneal fluid and serum of women with endometriosis in relation to stage of disease. Concentrations of neopterin and IL-10 were measured by enzyme linked immunosorbent assay in PF and serum of 58 women; 43 with endometriosis and 15 without endometriosis. In our study present of neopterin in PF of women with endometriosis whereas neopterin could not be detected in the control group. Neopterin serum concentrations were significantly higher in affected women compared with control. Significantly increased neopterin concentrations were observed in peritoneal fluid and serum of women with advanced endometriosis. The mean peritoneal fluid and serum IL-10 concentrations were significantly higher ainong studied women than control. In women with early endometriosis, significantly increased concentration of IL-10 in peritoneal fluid and serum was observed. In conclusions, both increased concentrations of neopterin and IL-10 in the peritoneal fluid and serum suggest an important role of these Compounds in pathogenesis of endometriosis and indicate an enhanced macrophage activity in this disease.

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Lithogenic Potential of Ureaplasma in Chronic Prostatitis

Urologia Internationalis ◽

10.1159/000511653 ◽

2020 ◽

pp. 1-6

Author(s):

Vittorio Magri ◽

Gianpaolo Perletti ◽

Konstantinos Stamatiou ◽

Emanuele Montanari ◽

Alberto Trinchieri

Keyword(s):

Pelvic Pain ◽

Chronic Prostatitis ◽

Chronic Pelvic Pain ◽

Chronic Pelvic Pain Syndrome ◽

Pain Syndrome ◽

Pelvic Pain Syndrome ◽

Control Group ◽

Causative Role ◽

Microscopic Evaluation

Introduction: The role of Ureaplasma spp. (UPs) in the pathogenesis of chronic prostatitis is debated. The lithogenic potential of UPs could be a risk factor for the development of chronic prostatitis. Methods: A total of 143 patients with identification of UPs were retrospectively selected from a database including patients with prostatitis-like symptoms who were studied according to the same protocol including clinical, microbiological and microscopic evaluation, and transrectal prostate ultrasound. A control group of patients with negative UPs was considered including 393 with chronic bacterial prostatitis (CBP), 42 patients with Chlamydia trachomatis (CT), and 781 patients with chronic pelvic pain syndrome. UPs and Mycoplasma hominis (MH) were identified using a semiquantitative assay. Results: Calcifications were observed more frequently in patients with UPs (64%) than in patients with CBP without UPs (39%), CT infection (37%), and chronic pelvic pain syndrome (29%) (p < 0.0001). UPs were isolated in VB1 alone in 35 patients (urethral UPs), in expressed prostatic secretion (EPS) or post-massage urine (VB3) or sperm in 77 patients (prostatic UPs) and associated with other pathogens in 31 patients (associated UPs). Calcifications were more frequent in prostatic UPs (71%) and associated UPs (73%) than in urethral UPs (34%). Mean NIH-CPSI scores were not significantly different between groups, although mean WBC counts of sperm of patients with urethral UPs were significantly lower than in patients with prostatic UPs (p = 0.000) and associated UPs (p = 0.002). Conclusions: UPs identification in the urogenital fluids is related to higher rates of prostate calcifications. The ability of UPs to promote the formation of calcifications could be related to the chronicization of prostate infection. In particular, the presence of UPs in VB3/EPS/sperm is associated with higher rates of calcifications and high WBC sperm counts, suggesting a partial or full causative role of UPs in the pathogenesis of this disease.

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Role of gram‐positive bacteria in chronic pelvic pain syndrome (CPPS)

The Prostate ◽

10.1002/pros.23721 ◽

2018 ◽

Vol 79 (2) ◽

pp. 160-167 ◽

Cited By ~ 3

Author(s):

Stephen F. Murphy ◽

Jonathan F. Anker ◽

Daniel J. Mazur ◽

Christel Hall ◽

Anthony J. Schaeffer ◽

...

Keyword(s):

Pelvic Pain ◽

Chronic Pelvic Pain ◽

Chronic Pelvic Pain Syndrome ◽

Pain Syndrome ◽

Pelvic Pain Syndrome ◽

Gram Positive ◽

Gram Positive Bacteria

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Role of bacteria in chronic prostatitis/ chronic pelvic pain syndrome

Current Prostate Reports ◽

10.1007/s11918-005-0021-5 ◽

2005 ◽

Vol 3 (2) ◽

pp. 87-93

Author(s):

Vi N. Hua ◽

Daniel H. Williams ◽

Anthony J. Schaeffer

Keyword(s):

Pelvic Pain ◽

Chronic Prostatitis ◽

Chronic Pelvic Pain ◽

Chronic Pelvic Pain Syndrome ◽

Pain Syndrome ◽

Pelvic Pain Syndrome

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Chronic pelvic pain syndrome: Role of a thorough clinical assessment

Scandinavian Journal of Urology ◽

10.3109/21681805.2014.961546 ◽

2014 ◽

Vol 49 (2) ◽

pp. 81-89 ◽

Cited By ~ 7

Author(s):

Jörgen Quaghebeur ◽

Jean-Jacques Wyndaele

Keyword(s):

Pelvic Pain ◽

Clinical Assessment ◽

Chronic Pelvic Pain ◽

Chronic Pelvic Pain Syndrome ◽

Pain Syndrome ◽

Pelvic Pain Syndrome

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Relevant issues of management of patients with inflammatory diseases of the pelvic organs

Infusion & Chemotherapy ◽

10.32902/2663-0338-2020-3.2-162-164 ◽

2020 ◽

pp. 162-164

Author(s):

І.V. Lakhno

Keyword(s):

Chronic Inflammation ◽

Inflammatory Diseases ◽

Chronic Pelvic Pain Syndrome ◽

Clinical Signs ◽

Resistance Index ◽

Pain Syndrome ◽

Pelvic Pain Syndrome ◽

Control Group ◽

Low Efficiency ◽

Rule Out

Background. Pelvic inflammatory diseases (PID) occur in 12-13 % of young women, 65-70 % of outpatients and 30 % of inpatients with gynecological diseases. The consequences of PID include infertility, chronic pelvic pain syndrome, menstrual disorders, etc. Treatment of PID is a multidisciplinary problem in the field of gynecology, urology, and venereology. Objective. To describe the modern treatment of PID. Materials and methods. Analysis of literature sources on this issue; own study to study the effectiveness of the PID treatment with Reosorbilact (“Yuria-Pharm”) and levofloxacin + ornidazole (Grandazole, “Yuria-Pharm”). Women of the main group were additionally prescribed fluconazole, diclofenac, vaginal baths with Dekasan (“Yuria-Pharm”). The treatment lasted 7 days. Treatment of the comparison group included ceftriaxone, metronidazole, diclofenac, doxycycline, fluconazole, chlorhexidine. Results and discussion. Chronic PID often have a latent course. 70 % of them are caused by the specific flora (Chlamydia trachomatis, Neisseria gonorrheae, anaerobes, gram-negative bacteria). The frequency of mixed polymicrobial processes and polychemical resistance is increasing. The presence of bacterial vaginosis allows the infections to recur constantly. Pathogens that cause PID can also cause extragenital pathological conditions (perihepatitis, Reiter’s syndrome, enteritis, colitis, cholecystitis). Diagnostic criteria for PID are the following: pain in the appendages or when the cervix is displaced during the bimanual examination, fever, leukorrhea and menorrhagia. If PID is suspected, a bimanual examination should be performed to rule out acute appendicitis. Ultrasound or computed tomography should be performed to rule out tuboovarian tumors and make a differential diagnosis with intestinal or urinary tract disease. The etiological diagnosis requires microbial and molecular examination of the contents of the vagina and cervix. Fluoroquinolones with metronidazole for 14 days are the first line therapy of uncomplicated PID. Chronic inflammation has no mechanisms of self-completion and can last for years and decades. In gynecology, chronic inflammation is divided into infectious, allergic and autoimmune type. Patients with recurrence of chronic PID are characterized by mixed infections and the formation of biofilms, allergies, low efficiency of immune cells. To overcome the polychemical resistance of pathogens, it is advisable to use effective hydrodynamic drugs that can act as a hydraulic conductor of the antibacterial agent, improve microcirculation in the inflammatory focus, optimize venous hemodynamics and lymphatic drainage. Sorbitol has all these properties. In addition, sorbitol increases the tropism of fluoroquinolones to gram-positive microorganisms and has own bacteriostatic effect. In the own study, it was found that the increase in the resistance index of the ovarian arteries was associated with increased intensity of pain in the lower abdomen, pathological vaginal discharge and fever, which justifies the use of vasoactive drugs in the treatment of PID. In the Reosorbilact and Grandazole treatment groups, normalization of clinical and laboratory parameters occurred in 100 % of women, whereas in 12.1 % of control group members the result was considered insufficient, requiring antibiotic replacement and continuation of therapy. Conclusions. 1. In women with PID, there is a connection between blood flow in the ovarian arteries and the severity of the clinical signs of PID, which justifies the use of hemodynamic drugs. 2. Improvement of intrapelvic hemodynamics on the background of Reosorbilact was the key to successful use of Grandazole. 3. Co-administration of Reosorbilact and Grandazole is a promising method of empirical therapy of PID.

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Analysis of the Effect of Emergency Ventilators on the Treatment of Critical Illness Based on Smart Medical Big Data

Journal of Healthcare Engineering ◽

10.1155/2021/7698769 ◽

2021 ◽

Vol 2021 ◽

pp. 1-10

Author(s):

Haiwang Sha ◽

Fen He

Keyword(s):

Respiratory Failure ◽

Critically Ill ◽

Critically Ill Patients ◽

Pulmonary Ventilation ◽

Clinical Manifestations ◽

Effective Rate ◽

Control Group ◽

Oxygen Balance ◽

Respiratory Muscle Fatigue

Respiratory failure refers to pulmonary ventilation and ventilatory dysfunction caused by various reasons, which makes the patient unable to maintain the gas exchange required for stillness and causes a series of pathophysiological changes and corresponding clinical manifestations. In order to solve the problem of respiratory failure in critically ill patients, it is of great significance to analyze the role of microprocessor-based emergency ventilator in the treatment of critically ill patients. This article aims to study the role of microprocessor-based emergency ventilator in the treatment of critically ill patients. This paper presents the key technology based on the ARM11 processor. A breathing motion model is detected and established through a ventilator. The research objects are mainly divided into group A and group B. By comparing the two groups of emergency ventilator ventilation, it can effectively prevent the increase in respiratory muscle fatigue, reduce oxygen consumption, improve the patient's ventilation function and oxygen balance, quickly correct hypoxia and carbon dioxide storage, cooperate with drug treatment, and quickly take out the ventilator after relief. Good treatment results were achieved. The results show that the emergency ventilator controlled by a microcomputer is effective. The total effective rate of the control group was 71.11%, which was significantly lower than that of the observation group (86.67%).

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CHANGES IN THE PARAMETERS OF THROMBODYNAMICS AND BLOOD CLOT CONTRACTION IN PATIENTS WITH RHEUMATOID ARTHRITIS

Rheumatology Science and Practice ◽

10.14412/1995-4484-2020-294-303 ◽

2020 ◽

Vol 58 (3) ◽

pp. 294-303

Author(s):

A. D. Peshkova ◽

T. A. Evdokimova ◽

T. B. Sibgatullin ◽

F. I. Ataullakhanov ◽

R. I. Litvinov

Keyword(s):

Rheumatoid Arthritis ◽

Blood Clot ◽

Clinical Manifestations ◽

Control Group ◽

Healthy Individuals ◽

Plasma Clot ◽

Autoimmune Pathology ◽

Clot Contraction ◽

Hemostatic Disorders

Autoimmune diseases, including rheumatoid arthritis (RA), are risk factors for thrombotic events. Understanding the pathogenetic role of hemostatic changes in RA can assist in developing measures for prevention, prognosis, early diagnosis, and treatment of immune thromboses. Objective: to investigate the state of platelet and plasma hemostasis in patients with RA, as compared to other laboratory parameters and clinical manifestations of the disease. Subjects and methods. Hemostasis was investigated using two relatively new laboratory tests: thrombodynamics and kinetics of blood clot contraction (BCC). Examinations were made in 60 patients with RA and in 50 apparently healthy individuals of the control group. Results and discussion. In patients with RA, the parameters of thrombodynamics and BCC were found to be significantly different from the normal values. According to thrombodynamics, there was an increase in plasma clot growth rate, size, and density, which indicates chronic hypercoagulation. The rate and completeness of BCC were substantially reduced due to platelet dysfunction in patients with RA compared to healthy individuals. The changes in the parameters of thrombodynamics and BCC correlated with the laboratory signs of systemic inflammation and depended on the radiographic stage of the disease. Conclusion. The results of this investigation confirm that hemostatic disorders are present in RA and indicate the informative value of thrombodynamics and BCC tests as indicators of a pre-thrombotic state, including autoimmune pathology.

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