Osteoblasts modulate calcium signaling in bone metastatic breast and prostate cancer cells

Taxol has been used as anti-cancer compound against prostate, ovarian, and metastatic breast cancer. While the most obvious effect of taxol is bundeling of microtubules and mitotic arrest, recent studies have demonstrated that taxol is able to induce intranucleosomal DNA fragmentation and typical morphological features of apoptosis in a number of solid tumor cells. These results indicate that taxol may exert its anti-tumor effects via secondary mechanisms which may or may not be related to its primary effects on microtubules. It has been shown that taxol-induced microtubular changes and G2/M arrest are associated with the release of the electron transfer protein cytochrome C from mitochondria into the cytosol. Cytochrome C then binds to APAF-1 (a human homolog of the ced-4 gene of C. elegans), which binds, cleaves, and activates caspase- 9, ultimately resulting in the cleavage and activity of caspase-3. We investigated the effects of taxol (100nM) on microtubules, on DNA, and on the pre-apoptotic mitochondrial events using LNCaP and DU145 prostate cancer cells.

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Inhibition of 17 -Hydroxysteroid Oxidoreductase by Flavonoids in Breast and Prostate Cancer Cells

Experimental Biology and Medicine ◽

10.3181/00379727-217-44237 ◽

1998 ◽

Vol 217 (3) ◽

pp. 310-316 ◽

Cited By ~ 91

Author(s):

S. Makela ◽

M. Poutanen ◽

M. L. Kostian ◽

N. Lehtimaki ◽

L. Strauss ◽

...

Keyword(s):

Prostate Cancer ◽

Cancer Cells ◽

Prostate Cancer Cells ◽

Breast And Prostate Cancer

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Molecular Decoy to the Y-Box Binding Protein-1 Suppresses the Growth of Breast and Prostate Cancer Cells whilst Sparing Normal Cell Viability

PLoS ONE ◽

10.1371/journal.pone.0012661 ◽

2010 ◽

Vol 5 (9) ◽

pp. e12661 ◽

Cited By ~ 22

Author(s):

Jennifer H. Law ◽

Yvonne Li ◽

Karen To ◽

Michelle Wang ◽

Arezoo Astanehe ◽

...

Keyword(s):

Prostate Cancer ◽

Cell Viability ◽

Cancer Cells ◽

Normal Cell ◽

Binding Protein ◽

Prostate Cancer Cells ◽

Breast And Prostate Cancer

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Proliferative effect of whey from cows’ milk varying in phyto-oestrogens in human breast and prostate cancer cells

Journal of Dairy Research ◽

10.1017/s0022029911000902 ◽

2012 ◽

Vol 79 (2) ◽

pp. 143-149 ◽

Cited By ~ 4

Author(s):

Tina S. Nielsen ◽

Annika Höjer ◽

Anne-Maj Gustavsson ◽

Jens Hansen-Møller ◽

Stig Purup

Keyword(s):

Prostate Cancer ◽

Cell Proliferation ◽

Cancer Cells ◽

Dietary Treatment ◽

Human Breast ◽

Prostate Cancer Cells ◽

Proliferative Effect ◽

Significant Difference ◽

Breast And Prostate Cancer ◽

Mcf 7

Intake of dietary phyto-oestrogens has received a great deal of attention owing to their potential influence on hormone-sensitive cancers such as breast and prostate cancer. Cows’ milk contains phyto-oestrogens and the content varies according to the composition of the feed and the type and amount of legumes used. In this study we evaluated the proliferative effect of milk (whey) with different phyto-oestrogen content in human breast (MCF-7) and prostate cancer cells (PC-3). Milk was obtained from cows fed either a birdsfoot trefoil-timothy silage based ration (B1) or two different red clover silage based diets (R1 and R2) resulting in total phyto-oestrogen contents of 403, 1659 and 1434 ng/ml for the B1, R1 and R2 diets, respectively. Whey was produced from the milk and added to cell culture medium in concentrations up to 10% for MCF-7 cells and 5% for PC-3 cells. Cell proliferation was measured fluorometrically after 7 d for MCF-7 cells and 5 d for PC-3 cells. There was no significant difference in the proliferative effect of whey from the different dietary treatments at any of the whey concentrations tested. An anti-proliferative effect (P<0·01) of 5 and 10% whey was seen when tested in the presence of 10 pmoestradiol in the medium. This effect was independent of dietary treatment of cows. Whey induced a significant (P<0·01) proliferative response in PC-3 cells independent of dietary treatment. Purified equol in concentrations similar to equol concentrations in milk decreased PC-3 cell proliferation, and therefore the stimulatory effect of whey in PC-3 cells is believed to be mediated by other bioactives than equol. In conclusion, our results suggest that using whey in these proliferation assays, it was not possible to discriminate between milk with high or low levels of phyto-oestrogens.

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