scholarly journals Resistance of DB1 Transgenic Rice Line and others against Nilaparvata lugens, Brown Planthopper

2021 ◽  
Vol 26 (01) ◽  
pp. 125-130
Author(s):  
Nono Carsono
Keyword(s):  
Transgenic Rice ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Crop Damage ◽  
Resistance Level ◽  
Rice Line ◽  
Transgenic Rice Line ◽  
Honeydew Excretion ◽  
Nilaparvata Lugens Stål ◽  
Moderately Resistant

Nilaparvata lugens (Stål), brown planthopper (BPH) is as a major pest of rice crop. Developing rice resistant to BPH is an economically and environmentally friendly approach. A transgenic rice line with DB1 (Dioscorea batatas tuber lectin 1) transgene has been engineered to overcome the problem. The objective of the experiment was to obtain the level of resistance of a DB1 rice line (DB1-inserted cv. Taichung 65) and some rice genotypes against two colonies of BPH. Resistance study was performed in BPH’s preference, honeydew excretion, crop damage, and population development. The resistant test was carried out using two biotypes BPH i.e., biotype 2 (Sukamandi) and biotype 3 (North Sumatera). The result showed that DB1 transgenic rice was moderately resistant to biotype 2, while to be susceptible when invested to biotype 3, indicating that the DB1 gene increases the resistance level, from susceptible to moderately resistant. All tested genotypes were chosen by biotype 2 for laying eggs, while for biotype 3 preferred five genotypes (DB1 transgenic line, PTB-33, Babawee, IR-64 dan IR-42). Genotype significantly differed on number of BPH and was considerably lower on PTB-33 than the others. Honeydew excretion of genotypes showed equal on biotype 2 and 3, except on biotype 3 of IR 42 (susceptible to BPH) which showing high amount of feces. PTB 33 had lower BPH population as compared to others, indicating high resistance to BPH of biotype 2 and 3 from Indonesia. © 2021 Friends Science Publishers

Variety-related feeding patterns in the brown planthopper, Nilaparvata lugens (stål) (Hemiptera: Delphacidae), on its host, the rice plant

1988 ◽  
Vol 78 (2) ◽  
pp. 339-349 ◽  
Author(s):  
D. E. Padgham ◽  
S. Woodhead
Keyword(s):  
Rice Variety ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Time Of Day ◽  
Rice Varieties ◽  
Sources Of Variation ◽  
Insect Populations ◽  
Honeydew Excretion ◽  
Nilaparvata Lugens Stål ◽  
Moderately Resistant

AbstractMajor potential sources of variation in the feeding of Nilaparvata lugens (Stål) which are not host-dependant are those related to time, i.e. the age of the insect, the length of time the insect is in association with the plant and the time of day. These parameters were investigated with a susceptible, a moderately resistant and a resistant rice variety using honeydew clocks for the collection of honeydew excretion data. It is concluded that there are non-circadian patterns of honeydew excretion which are related to the degree of resistance of the variety and that such feeding and excretion patterns can be used to characterize the relationship between insect populations and rice varieties.

A new gene Bph33(t) conferring resistance to brown planthopper (BPH), Nilaparvata lugens (Stål) in rice line RP2068-18-3-5

Euphytica ◽  
2018 ◽  
Vol 214 (3) ◽  
Author(s):  
Sabhavat Bhaskar Naik ◽  
Dhanasekar Divya ◽  
Nihar Sahu ◽  
Raman Meenakshi Sundaram ◽  
Preetinder Singh Sarao ◽  
...  
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Rice Line ◽  
New Gene ◽  
Nilaparvata Lugens Stål

scholarly journals Antibiosis effects of rice carrying Bph14 and Bph15 on the brown planthopper, Nilaparvata lugens

2019 ◽  
Author(s):  
Liangmiao Qiu ◽  
Wuqi Wang ◽  
Longqing Shi ◽  
Qiquan Liu ◽  
Zhixiong Zhan
Keyword(s):  
Insect Pest ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Test Method ◽  
Host Feeding ◽  
Developmental Duration ◽  
Resistance Level ◽  
Female Ratio ◽  
Emergence Rate ◽  
Honeydew Excretion

The brown planthopper (BPH), Nilaparvata lugens, is the most destructive insect pest in rice production worldwide. The development and cultivation of BPH-resistant varieties is the most economical and efficient strategy to overcome the destruction caused by BPH. In this study, the modified bulk seedling test method was used to identify the BPH resistance level and host feeding choice of rice lines of Liangyou8676 (Bph14/Bph15), Bph68S (Bph14/Bph15), RHT (Bph3), Fuhui676, and TN1 on BPH. Meanwhile, the population, survival and emergence rate, developmental duration, honeydew excretion, female ratio and brachyptery ratio of adults were used as indicators to detect the antibiosis effects of the different rice lines. The results showed that the resistance levels of Rathu Heenati (RHT), Bph68S, Liangyou8676, Fuhui676, and TN1 to BPH were HR, R, MR, S and HS, respectively. The host choice implied that BPH was more inclined to feeding on rice plants with a lower resistance. An analysis of the antibiosis activity of rice lines RHT, BPh68S, and Liangyou8676 carrying resistance genes indicated a significant reduction in the population growth rate, survival and emergence rate of BPH nymphs, significant delay in the developmental duration of nymphs, reduced honeydew excretion of females, decreased female ratio, and a decreased brachyptery ratio of females and males, when compared with rice carrying no BPH-resistant genes.

scholarly journals Antibiosis Effects of Rice Carrying Bph14 and Bph15 on the Brown Planthopper, Nilaparvata lugens

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 109
Author(s):  
Liangmiao Qiu ◽  
Wuqi Wang ◽  
Longqing Shi ◽  
Qiquan Liu ◽  
Zhixiong Zhan
Keyword(s):  
Insect Pest ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Test Method ◽  
Host Feeding ◽  
Developmental Duration ◽  
Resistance Level ◽  
Female Ratio ◽  
Emergence Rate ◽  
Honeydew Excretion

The brown planthopper(BPH), Nilaparvata lugens, is the most destructive insect pest in rice production worldwide. The development and cultivation of BPH-resistant varieties is the most economical and efficient strategy to overcome the destruction caused by BPH. The modified bulk seedling test method was used to identify the BPH resistance level and host feeding choice of rice lines of Liangyou8676(Bph14/Bph15), Bph68S(Bph14/Bph15), RHT(Bph3), Fuhui676, and TN1 on BPH. In the meantime, the population, survival and emergence rate, developmental duration, honeydew excretion, female ratio and brachypterous ratio of adults were used as indicators to detect the antibiosis effects of the different rice lines. The results showed that the resistance levels of RHT, Bph68S, Liangyou8676, Fuhui676, and TN1 to BPH were HR, R, MR, S and HS, respectively. The host choice implied that BPH was more inclined to feeding on rice plants with a lower resistance. An analysis of the antibiosis activity of rice lines RHT, BPh68S, and Liangyou8676 carrying resistance genes indicated a significant reduction in the population growth rate, survival and emergence rate of BPH nymphs, significant delay in the developmental duration of nymphs, reduced honeydew excretion of females, decreased female ratio, and a decreased brachypterous ratio of females and males, when compared with rice carrying no BPH-resistant genes.

scholarly journals Stability of Resistance to Imidacloprid in the Brown Planthopper (Nilaparvata lugens Stål.) from Banyumas, Central Java

2020 ◽  
Vol 24 (1) ◽  
pp. 61
Author(s):  
Danarsi Diptaningsari ◽  
Y. Andi Trisyono ◽  
Aziz Purwantoro ◽  
Arman Wijonarko
Keyword(s):  
Brown Planthopper ◽  
Management Strategies ◽  
Resistance Management ◽  
Nilaparvata Lugens ◽  
Resistance Level ◽  
Central Java ◽  
Fifth Generation ◽  
Stability Of Resistance ◽  
The Stability ◽  
Nilaparvata Lugens Stål

Imidacloprid is one of the insecticides that has been widely used to control the brown planthopper (Nilaparvata lugens Stål.). The excessive use of insecticides led to the development of insecticide resistance in N. lugens. This study was aimed to determine the stability of resistance to imidacloprid in N. lugens from Banyumas, Central Java Province. The five generations of selection increased the resistance ratio (RR) from 46.20-fold to 150.39-fold. To assess the stability of resistance to imidacloprid in N. lugens, the fifth generation was further reared for 10 generations without exposure to insecticides. The stability studies showed that resistance to imidacloprid was unstable with a decrease of resistance from 150.39-fold to 38.14-fold after 10 generations without selections. Continuous selections with imidacloprid for three generations could increase the resistance level from 150.39-fold to 216.13-fold. The unstable resistance could be managed by removing the selection pressure for a period of time or switching to insecticides with different mode of actions. Information regarding resistance stability would be useful to determine an effective resistance management strategies in N. lugens.

scholarly journals Antibiosis effects of rice carrying Bph14 and Bph15 on the brown planthopper, Nilaparvata lugens

2019 ◽  
Author(s):  
Liangmiao Qiu ◽  
Wuqi Wang ◽  
Longqing Shi ◽  
Qiquan Liu ◽  
Zhixiong Zhan
Keyword(s):  
Insect Pest ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Test Method ◽  
Host Feeding ◽  
Developmental Duration ◽  
Resistance Level ◽  
Female Ratio ◽  
Emergence Rate ◽  
Honeydew Excretion

The brown planthopper (BPH), Nilaparvata lugens, is the most destructive insect pest in rice production worldwide. The development and cultivation of BPH-resistant varieties is the most economical and efficient strategy to overcome the destruction caused by BPH. In this study, the modified bulk seedling test method was used to identify the BPH resistance level and host feeding choice of rice lines of Liangyou8676 (Bph14/Bph15), Bph68S (Bph14/Bph15), RHT (Bph3), Fuhui676, and TN1 on BPH. Meanwhile, the population, survival and emergence rate, developmental duration, honeydew excretion, female ratio and brachyptery ratio of adults were used as indicators to detect the antibiosis effects of the different rice lines. The results showed that the resistance levels of Rathu Heenati (RHT), Bph68S, Liangyou8676, Fuhui676, and TN1 to BPH were HR, R, MR, S and HS, respectively. The host choice implied that BPH was more inclined to feeding on rice plants with a lower resistance. An analysis of the antibiosis activity of rice lines RHT, BPh68S, and Liangyou8676 carrying resistance genes indicated a significant reduction in the population growth rate, survival and emergence rate of BPH nymphs, significant delay in the developmental duration of nymphs, reduced honeydew excretion of females, decreased female ratio, and a decreased brachyptery ratio of females and males, when compared with rice carrying no BPH-resistant genes.

scholarly journals Phenotypic Screening of Elite Rice Germplasms against Brown Planthopper [Nilaparvata lugens (Stal.)] for Identification of Resistant Donor Lines

2019 ◽  
pp. 1-7
Author(s):  
M. Nikhil Raj ◽  
P. Rajani Kanth ◽  
N. R. G. Varma ◽  
M. Balram
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Research Centre ◽  
Randomized Design ◽  
Resistant Lines ◽  
Rice Genotypes ◽  
Underlying Mechanisms ◽  
Agriculture Research Institute ◽  
Nilaparvata Lugens Stål ◽  
Moderately Resistant

Aims: To identify Brown Planthopper (BPH) resistant rice genotypes and categorize all the test entries based on their level of resistance against BPH. Study Design: Completely Randomized Design. Place and Duration of Study: Poly-house, Department of Entomology, Rice Research Centre, Agriculture Research Institute (ARI), Rajendranagar, Hyderabad, India, between June 2016 and July 2017. Methodology: A total of 61 elite rice genotypes selected including resistant (PTB33) and susceptible check (TN1). All these test entries were screened against Brown Planthopper (BPH) using Standard Seedbox Screening Technique (SSST) inside poly-house conditions. Based on the Damage Score (DS) achieved during study, all entries were categorized into resistant (R), moderately resistant (MR), moderately susceptible (MS), susceptible (S) and highly susceptible (HS). Results: Among 61 cultures, the resistant check PTB33 and BM71 exhibited R reaction to BPH with a DS of 3.0, while twelve cultures viz. Milyang 63, IET 23993, HHZ 5 DT-1 DT-1, HHZ 25 SAL DT-1 DT-1, Bobhu Kongbu, BPT 2671, BPT 2611, MTU 1121, MTU 1001, MTU 1010, RNR 23079 and GSR 234 exhibited MR reaction to BPH with a DS ranging between 3.1 to 5.0. The rest of the cultures showed MS and S reactions while the susceptible check, TN1 along with other 12 cultures exhibited HS reaction to BPH with a DS of 9.0. Conclusion: Resistant and moderately resistant rice genotypes have been identified for development of BPH resistant lines. Further detailed studies are required to understand the underlying mechanisms of resistance among the R and MR genotypes.

scholarly journals Resistance Evaluation of Some Rice Varieties by Feeding Activity of Brown Planthopper Population in Java

2021 ◽  
Vol 5 (2) ◽  
pp. 79-87
Author(s):  
Willing Bagariang ◽  
Wayan Murdita ◽  
Suwarman ◽  
Ahmad Imroni
Keyword(s):  
Feeding Activity ◽  
Brown Planthopper ◽  
Similarity Index ◽  
Nilaparvata Lugens ◽  
Rice Varieties ◽  
Resistance Level ◽  
Central Java ◽  
Important Pest ◽  
And Cluster Analysis ◽  
Nilaparvata Lugens Stål

Brown Planthopper (BPH), Nilaparvata lugens Stal (Hemiptera: Delpachidae) is the important pest of rice. The infestation of BPH reduced the rice yield on rice producer countries in Asia. In Indonesia, the area of BPH infestation frequently fluctuated over the year. BPH directly caused hopper burn and also vector of rice grassy stunt virus (RGSV) and rice ragged stunt virus (RRSV). This study aimed to evaluate the resistance of some rice varieties to different BPH population in Java. Three BPH populations collected from Karawang (West Java), Pekalongan (Central Java) and Kendal (Central Java) were used in this study. The rice resistance was evaluated by conducting honeydew test. There were eight rice varieties tested i.e Sembada 168, Ciherang, Inpari 32, Inpari 33, IR 64, TN1, Ratu Heenati and PTB 33 with five replications. A number of 200 female adults (five females per plot) were starved for 2 hours before inserting to pot containing one seedling.  The BPH adults were allowed to feed 40-day-old plants for 48 hours. The area of honeydew excreted by BPH was measured using software Image J. We used analysis of variance (ANOVA) and cluster analysis with ward method and similarity index of Euclidean distance to analyze the rice resistance to BPH. The study indicated three groups of rice resistance level. The variety of Sembada 168 and TN1 were susceptible to BPH. Variety of Ciherang, Inpari 32, Inpari 33 and IR 64 were moderately resistant to BPH. However, PTB 33 and Ratu Heenati were strongly resistant to BPH population of Java.

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