scholarly journals Antibiosis Effects of Rice Carrying Bph14 and Bph15 on the Brown Planthopper, Nilaparvata lugens

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 109
Author(s):  
Liangmiao Qiu ◽  
Wuqi Wang ◽  
Longqing Shi ◽  
Qiquan Liu ◽  
Zhixiong Zhan
Keyword(s):  
Insect Pest ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Test Method ◽  
Host Feeding ◽  
Developmental Duration ◽  
Resistance Level ◽  
Female Ratio ◽  
Emergence Rate ◽  
Honeydew Excretion

The brown planthopper(BPH), Nilaparvata lugens, is the most destructive insect pest in rice production worldwide. The development and cultivation of BPH-resistant varieties is the most economical and efficient strategy to overcome the destruction caused by BPH. The modified bulk seedling test method was used to identify the BPH resistance level and host feeding choice of rice lines of Liangyou8676(Bph14/Bph15), Bph68S(Bph14/Bph15), RHT(Bph3), Fuhui676, and TN1 on BPH. In the meantime, the population, survival and emergence rate, developmental duration, honeydew excretion, female ratio and brachypterous ratio of adults were used as indicators to detect the antibiosis effects of the different rice lines. The results showed that the resistance levels of RHT, Bph68S, Liangyou8676, Fuhui676, and TN1 to BPH were HR, R, MR, S and HS, respectively. The host choice implied that BPH was more inclined to feeding on rice plants with a lower resistance. An analysis of the antibiosis activity of rice lines RHT, BPh68S, and Liangyou8676 carrying resistance genes indicated a significant reduction in the population growth rate, survival and emergence rate of BPH nymphs, significant delay in the developmental duration of nymphs, reduced honeydew excretion of females, decreased female ratio, and a decreased brachypterous ratio of females and males, when compared with rice carrying no BPH-resistant genes.

scholarly journals Antibiosis effects of rice carrying Bph14 and Bph15 on the brown planthopper, Nilaparvata lugens

2019 ◽  
Author(s):  
Liangmiao Qiu ◽  
Wuqi Wang ◽  
Longqing Shi ◽  
Qiquan Liu ◽  
Zhixiong Zhan
Keyword(s):  
Insect Pest ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Test Method ◽  
Host Feeding ◽  
Developmental Duration ◽  
Resistance Level ◽  
Female Ratio ◽  
Emergence Rate ◽  
Honeydew Excretion

The brown planthopper (BPH), Nilaparvata lugens, is the most destructive insect pest in rice production worldwide. The development and cultivation of BPH-resistant varieties is the most economical and efficient strategy to overcome the destruction caused by BPH. In this study, the modified bulk seedling test method was used to identify the BPH resistance level and host feeding choice of rice lines of Liangyou8676 (Bph14/Bph15), Bph68S (Bph14/Bph15), RHT (Bph3), Fuhui676, and TN1 on BPH. Meanwhile, the population, survival and emergence rate, developmental duration, honeydew excretion, female ratio and brachyptery ratio of adults were used as indicators to detect the antibiosis effects of the different rice lines. The results showed that the resistance levels of Rathu Heenati (RHT), Bph68S, Liangyou8676, Fuhui676, and TN1 to BPH were HR, R, MR, S and HS, respectively. The host choice implied that BPH was more inclined to feeding on rice plants with a lower resistance. An analysis of the antibiosis activity of rice lines RHT, BPh68S, and Liangyou8676 carrying resistance genes indicated a significant reduction in the population growth rate, survival and emergence rate of BPH nymphs, significant delay in the developmental duration of nymphs, reduced honeydew excretion of females, decreased female ratio, and a decreased brachyptery ratio of females and males, when compared with rice carrying no BPH-resistant genes.

scholarly journals Antibiosis effects of rice carrying Bph14 and Bph15 on the brown planthopper, Nilaparvata lugens

2019 ◽  
Author(s):  
Liangmiao Qiu ◽  
Wuqi Wang ◽  
Longqing Shi ◽  
Qiquan Liu ◽  
Zhixiong Zhan
Keyword(s):  
Insect Pest ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Test Method ◽  
Host Feeding ◽  
Developmental Duration ◽  
Resistance Level ◽  
Female Ratio ◽  
Emergence Rate ◽  
Honeydew Excretion

The brown planthopper (BPH), Nilaparvata lugens, is the most destructive insect pest in rice production worldwide. The development and cultivation of BPH-resistant varieties is the most economical and efficient strategy to overcome the destruction caused by BPH. In this study, the modified bulk seedling test method was used to identify the BPH resistance level and host feeding choice of rice lines of Liangyou8676 (Bph14/Bph15), Bph68S (Bph14/Bph15), RHT (Bph3), Fuhui676, and TN1 on BPH. Meanwhile, the population, survival and emergence rate, developmental duration, honeydew excretion, female ratio and brachyptery ratio of adults were used as indicators to detect the antibiosis effects of the different rice lines. The results showed that the resistance levels of Rathu Heenati (RHT), Bph68S, Liangyou8676, Fuhui676, and TN1 to BPH were HR, R, MR, S and HS, respectively. The host choice implied that BPH was more inclined to feeding on rice plants with a lower resistance. An analysis of the antibiosis activity of rice lines RHT, BPh68S, and Liangyou8676 carrying resistance genes indicated a significant reduction in the population growth rate, survival and emergence rate of BPH nymphs, significant delay in the developmental duration of nymphs, reduced honeydew excretion of females, decreased female ratio, and a decreased brachyptery ratio of females and males, when compared with rice carrying no BPH-resistant genes.

scholarly journals Resistance of DB1 Transgenic Rice Line and others against Nilaparvata lugens, Brown Planthopper

2021 ◽  
Vol 26 (01) ◽  
pp. 125-130
Author(s):  
Nono Carsono
Keyword(s):  
Transgenic Rice ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Crop Damage ◽  
Resistance Level ◽  
Rice Line ◽  
Transgenic Rice Line ◽  
Honeydew Excretion ◽  
Nilaparvata Lugens Stål ◽  
Moderately Resistant

Nilaparvata lugens (Stål), brown planthopper (BPH) is as a major pest of rice crop. Developing rice resistant to BPH is an economically and environmentally friendly approach. A transgenic rice line with DB1 (Dioscorea batatas tuber lectin 1) transgene has been engineered to overcome the problem. The objective of the experiment was to obtain the level of resistance of a DB1 rice line (DB1-inserted cv. Taichung 65) and some rice genotypes against two colonies of BPH. Resistance study was performed in BPH’s preference, honeydew excretion, crop damage, and population development. The resistant test was carried out using two biotypes BPH i.e., biotype 2 (Sukamandi) and biotype 3 (North Sumatera). The result showed that DB1 transgenic rice was moderately resistant to biotype 2, while to be susceptible when invested to biotype 3, indicating that the DB1 gene increases the resistance level, from susceptible to moderately resistant. All tested genotypes were chosen by biotype 2 for laying eggs, while for biotype 3 preferred five genotypes (DB1 transgenic line, PTB-33, Babawee, IR-64 dan IR-42). Genotype significantly differed on number of BPH and was considerably lower on PTB-33 than the others. Honeydew excretion of genotypes showed equal on biotype 2 and 3, except on biotype 3 of IR 42 (susceptible to BPH) which showing high amount of feces. PTB 33 had lower BPH population as compared to others, indicating high resistance to BPH of biotype 2 and 3 from Indonesia. © 2021 Friends Science Publishers

Quantification of antibiosis levels in nine different rice genotypes against Nilaparvata lugens (Homoptera: Delphacidae)

2018 ◽  
Vol 38 (04) ◽  
pp. 330-339
Author(s):  
P. S. Sarao ◽  
J. S. Bentur
Keyword(s):  
Brown Planthopper ◽  
Growth Index ◽  
Resistance Breeding ◽  
Nilaparvata Lugens ◽  
Female Population ◽  
Damage Score ◽  
Survival Percentage ◽  
Honeydew Excretion ◽  
Rice Genotypes ◽  
Better Than

AbstractStudies on different parameters of antibiosis to simultaneously compare diverse genotypes with different resistance levels are lacking. Such studies are necessary to identify the genetic variability among resistant genotypes, which, ultimately, can lead to the identification of resistance genes with diverse mechanisms. The current study was undertaken to quantify antibiosis levels in nine rice genotypes against the brown planthopper (BPH), Nilaparvata lugens (Stål), under glasshouse conditions using nine parameters. Among these genotypes, RP2068-18-3-5 and Ptb33 displayed significantly better performance as compared to other genotypes in most of the parameters studied and did not differ from each other. Rathu Heenati was the best in unfertilised eggs laid and similar to the above two genotypes regarding percent of nymphal survival. All the test genotypes performed significantly better than the susceptible check TN1, though INRC3021 did not differentiate from the former in some tests. The overall damage score was positively and significantly correlated with the amount of honeydew excretion, percent nymphal survival and emergence, the proportion of brachypterous females, female population, and growth index, and were negatively and significantly correlated with percent unfertilised eggs laid and nymphal development period. Regression analysis revealed a significant linear relationship between damage score and nymphal emergence, nymphal survival percentage, and proportion of brachypterous females. RP2068-18-3-5 is the new source of BPH resistance identified through this study, which breeders can further use in resistance breeding.

scholarly journals Recent Strategies for Detection and Improvement of Brown Planthopper Resistance Genes in Rice: A Review

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1202
Author(s):  
Bello Sani Haliru ◽  
Mohd Y. Rafii ◽  
Norida Mazlan ◽  
Shairul Izan Ramlee ◽  
Isma’ila Muhammad ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Large Scale ◽  
Insect Pest ◽  
Durable Resistance ◽  
Brown Planthopper ◽  
Gene Clusters ◽  
Nilaparvata Lugens ◽  
Scale Production ◽  
Rice Varieties ◽  
Resistant Genes

Brown planthopper (BPH; Nilaparvata lugens Stal) is considered the main rice insect pest in Asia. Several BPH-resistant varieties of rice have been bred previously and released for large-scale production in various rice-growing regions. However, the frequent surfacing of new BPH biotypes necessitates the evolution of new rice varieties that have a wide genetic base to overcome BPH attacks. Nowadays, with the introduction of molecular approaches in varietal development, it is possible to combine multiple genes from diverse sources into a single genetic background for durable resistance. At present, above 37 BPH-resistant genes/polygenes have been detected from wild species and indica varieties, which are situated on chromosomes 1, 3, 4, 6, 7, 8, 9, 10, 11 and 12. Five BPH gene clusters have been identified from chromosomes 3, 4, 6, and 12. In addition, eight BPH-resistant genes have been successfully cloned. It is hoped that many more resistance genes will be explored through screening of additional domesticated and undomesticated species in due course.

scholarly journals Characterization of gustatory receptor 7 in the brown planthopper reveals functional versatility

2020 ◽  
Author(s):  
Abhishek Ojha ◽  
Wenqing Zhang
Keyword(s):  
Insect Pests ◽  
Insect Pest ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Receptor Protein ◽  
Gustatory Receptor ◽  
Protein Coding ◽  
Nutrient Sources ◽  
Functional Studies ◽  
Protein Ligands

AbstractInsect pests consume tastants as their necessary energy and nutrient sources. Gustatory receptors play important roles in insect life and can form within an extremely complicated regulatory network. However, there are still many gustatory genes that have a significant impact on insect physiology, but their functional mechanism is still unknown. Here, we purified and characterized a gustatory receptor (protein) coding gene, NlGr7, from the brown planthopper (BPH) Nilaparvata lugens, which is an important insect pest of rice. Our results revealed that NlGr7 has an active association with various ligands, such as lectins, lipids (phospho- and sphingolipid) and copper. The mass-spectrometry result showed that NlGr7 is a sugar receptor, and NlGr7 is validated by different types of insoluble polysaccharides and a varied range of tastants. Furthermore, we observed that NlGr7-bound ATP hydrolysed on the ATPase activity assay, which indicated that NlGr7 may be associated with important biological functions in the BPH. The important NlGr7 for chemoreception has now been characterized in the BPH. We showed that NlGr7 in the BPH is required for various protein-ligands, as well as protein-sugars interactions, to play crucial roles in this pest. This study will provide valuable information for further functional studies of chemoreception mechanisms in this important agricultural pest.

scholarly journals NADPH Oxidase 5 Is Essential for Molting and Oviposition in a Rice Planthopper Nilaparvata lugens

Insects ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 642
Author(s):  
Lu-Yao Peng ◽  
Zhen-Wei Dai ◽  
Rui-Rui Yang ◽  
Zhen Zhu ◽  
Wei Wang ◽  
...  
Keyword(s):  
Rna Interference ◽  
Nadph Oxidase ◽  
Insect Pest ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Leaf Sheath ◽  
Whole Genome Sequence ◽  
First Choice ◽  
Nadph Oxidase 5

The brown planthopper Nilaparvata lugens is a typical monophagous insect herbivore that feeds exclusively on rice sap. This insect pest causes serious damage to rice crops throughout East Asian countries. Chemical control remains the first choice for managing N. lugens populations; however, the use of insecticides has given rise to planthopper resurgence and additional environmental risks. Nilaparvata lugens is a model insect of Hemiptera because its whole genome sequence has been elucidated and is susceptible to RNA interference. In this study, our findings revealed that a superoxide-generating gene, NADPH oxidase 5 (Nox5), is essential for molting and oviposition in a Hemipteran insect Nilaparvata lugens. Knockdown of Nox5 transcript levels by RNA interference in 2nd–5th-instar nymphs results in significantly lethal deficits in the molting transitions from nymph–nymph and nymph–adult. Nox5 knockdown leads to a reduction of hydrogen peroxide in female ovaries and failure of oviposition from the insect ovipositor into the rice leaf sheath. Here, we provide in vivo evidence demonstrating that Nox5 is a key enzyme for regulating molting and oviposition in this insect species.

scholarly journals Uji Ekstrak Daun Tithonia diversifolia sebagai Penghambat Daya Makan Nilaparvata lugens Stal. pada Oryza sativa L. (Evaluation of Tithonia diversifolia Leaf Extract as Feeding Capacity Inhibitor of Nilaparvata lugens in Oryza sativa L.)

2013 ◽  
Vol 3 (2) ◽  
Author(s):  
Tri A. Mokodompit ◽  
Roni Koneri ◽  
Parluhutan Siahaan ◽  
Agustina M Tangapo
Keyword(s):  
Oryza Sativa ◽  
Leaf Extract ◽  
Oryza Sativa L ◽  
Feeding Activity ◽  
Insect Pest ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Tithonia Diversifolia ◽  
Feeding Inhibition ◽  
Nilaparvata Lugens Stål

AbstrakWereng Batang Coklat (WBC) (Nilaparvata lugens Stal.) merupakan serangga hama yang dapat merusak tanaman padi (Oryza sativa L.). Tanaman kipait (Tithonia diversifolia) berpotensi sebagai insektisida nabati karena memiliki senyawa toksik terhadap serangga. Penelitian ini bertujuan untuk mengkaji aktifitas makan akibat pemberian ekstrak daun kipait. Konsentrasi ekstrak daun kipait yang digunakan adalah 0% (kontrol), 1%, 3%,5% dan 7% dalam RAL (Rancangan Acak Lengkap) dengan 5 kali ulangan. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun kipait berpengaruh terhadap penghambatan daya makan WBC. Penghambatan makan tertinggi terjadi pada konsentrasi 7% setelah 24 jam.Kata kunci : penghambatan daya makan, Nilaparvata lugens Stal., Tithonia diversifoliaAbstractBrown planthopper (BPH) (Nilaparvata lugens Stal.) Is an insect pest that can damage rice plants (Oryza sativa L.). Kipait (Tithonia diversifolia) is potential as a bioinsecticide because it is toxic to insects. This study aimed to evaluate the feeding activity that was influenced by kipait leaf extract. The concentration of kipait leaf extract were 0% (control), 1%, 3%, 5% and 7%. The experiment design was CRD (completely randomized design) with 5 replications. The results showed that the kipait leaf extract influenced the feeding inhibition of BHP. The highest inhibition occurred in the concentration of 7% after 24 hours treatment.Keywords : brown planthopper, feeding inhibition, Tithonia diversifolia

scholarly journals Biological Traits of the Pincer Wasp Gonatopus flavifemur (Esaki & Hashimoto) Associated with Different Stages of Its Host, the Brown Planthopper, Nilaparvata lugens (Stål)

Insects ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 279
Author(s):  
Jiachun He ◽  
Yuting He ◽  
Fengxiang Lai ◽  
Xiangsheng Chen ◽  
Qiang Fu
Keyword(s):  
Brown Planthopper ◽  
Female Offspring ◽  
Nilaparvata Lugens ◽  
Biological Traits ◽  
Host Feeding ◽  
Feeding Rates ◽  
Functional Responses ◽  
Male Adult ◽  
Offspring Development ◽  
Nilaparvata Lugens Stål

Gonatopus flavifemur (Esaki & Hashimoto) is a common parasitoid of the most important rice pest, the brown planthopper (BPH) Nilaparvata lugens (Stål), in eastern and southeastern Asia. We investigated the parasitism rates, feeding rates, and offspring development of G. flavifemur in association with five instars of BPH nymphs and male and female adults under laboratory conditions (27 ± 1 °C and 70 ± 5% Relative Humidity). The results showed that the life stage of the host significantly affects parasitism, host feeding, and offspring development by G. flavifemur. The parasitism rate was highest on 4th instar nymphs, and the feeding rate was highest on 1st instar nymphs. The cocooning rate on male adult BPHs was significantly lower than that on other stages; however, emergence rates did not significantly differ among the BPH stages. The rate of female offspring upon emergence from 5th instars was higher than other stages. Both the parasitism and host-feeding functional responses of G. flavifemur to different BPH stages fit well with Holling type II models, supporting the results of parasitism and feeding rates and indicating that G. flavifemur would be a good agent for BPH control. In conclusion, G. flavifemur prefers to feed on young nymphs but prefers to parasitize older nymphs. In addition, 5th instar nymphs are favorable to female offspring of the pincer wasp.

Variety-related feeding patterns in the brown planthopper, Nilaparvata lugens (stål) (Hemiptera: Delphacidae), on its host, the rice plant

1988 ◽  
Vol 78 (2) ◽  
pp. 339-349 ◽  
Author(s):  
D. E. Padgham ◽  
S. Woodhead
Keyword(s):  
Rice Variety ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Time Of Day ◽  
Rice Varieties ◽  
Sources Of Variation ◽  
Insect Populations ◽  
Honeydew Excretion ◽  
Nilaparvata Lugens Stål ◽  
Moderately Resistant

AbstractMajor potential sources of variation in the feeding of Nilaparvata lugens (Stål) which are not host-dependant are those related to time, i.e. the age of the insect, the length of time the insect is in association with the plant and the time of day. These parameters were investigated with a susceptible, a moderately resistant and a resistant rice variety using honeydew clocks for the collection of honeydew excretion data. It is concluded that there are non-circadian patterns of honeydew excretion which are related to the degree of resistance of the variety and that such feeding and excretion patterns can be used to characterize the relationship between insect populations and rice varieties.

Sign in / Sign up

Export Citation Format

Share Document