Research on microbial lipid production from potato starch wastewater as culture medium by Lipomyces starkeyi

2013 ◽  
Vol 67 (8) ◽  
pp. 1802-1808 ◽  
Author(s):  
Jun-Xian Liu ◽  
Qin-Yan Yue ◽  
Bao-Yu Gao ◽  
Yan Wang ◽  
Qian Li ◽  
...  
Keyword(s):  
Cell Growth ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Culture Medium ◽  
Potato Starch ◽  
Inoculum Size ◽  
Microbial Lipid ◽  
Lipomyces Starkeyi ◽  
Starch Wastewater ◽  
Source Concentration

In this paper, potato starch wastewater as culture medium was treated by the oleaginous yeast Lipomyces starkeyi to biosynthesize microbial lipid. The result indicated that carbon source types, carbon source concentration, nitrogen source types, nitrogen source concentration, inoculum size, and cultivation time all had a significant effect on cell growth and microbial lipid accumulation in batch cultures. A measure of 120 g/L of glucose concentration, 3.0 g/L of (NH4)2SO4 concentration, 10% inoculum size, and incubation time 96 h cultivated in a shaking flask at 30 °C were found to be the optimal conditions not only for cell growth but also for lipid synthesis. Under this condition, the cellular biomass and lipid content could reach 2.59 g/L and 8.88%, respectively. This work provides a new method for effective utilization of potato starch wastewater, which has particular social and economic benefits for yeast treatment technology.

Cell growth and pigment production in suspension cultures of a mycobiont isolated from the lichen Cladonia cristaiella

1995 ◽  
Vol 73 (S1) ◽  
pp. 590-594 ◽  
Author(s):  
Y. Yamamoto ◽  
Y. Kinoshita ◽  
T. Kurokawa ◽  
I. Yoshimura ◽  
V. Ahmadjian ◽  
...  
Keyword(s):  
Cell Growth ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Liquid Culture ◽  
Pigment Production ◽  
Malt Extract ◽  
Nutritional Factors ◽  
Cultural Conditions ◽  
Acetone Extracts ◽  
Affect Cell Growth

This is the first study on the factors that affect cell growth and the production of secondary metabolites of a lichen mycobiont in liquid culture. An ascospore-derived strain of Cladonia cristatella mycobiont accumulated and excreted red pigments into a liquid medium. Growth of the mycobiont was increased by using liquid Lilly–Barnett medium containing 16% (w/v) sucrose as a carbon source, 0.2% (w/v) L-glutamine as a nitrogen source, and 0.2% (w/v) polypeptone, adjusting pH to 5.0 before autoclaving, and incubating cultures at 20 °C. Pigment production by the mycobiont was increased by using liquid Lilly–Barnett medium containing 4% (w/v) sucrose as a carbon source, 0.2% (w/v) L-asparagine as a nitrogen source, and 0.2% (w/v) malt extract, adjusting pH to 5.0 before autoclaving and incubating cultures at 20 °C. All acetone extracts under any cultural conditions yielded similar HPLC chromatograms. We proved no relationship between cell growth and secondary metabolism based on the nutritional factors in the cultured C. cristatella mycobiont. Key words: lichen, suspension culture, Cladonia cristatella mycobiont, red pigment, production, and growth factor.

Observations préliminaires sur l'accumulation d'hydrocarbures chez Candida steatolytica: relation avec la synthèse des lipides

1985 ◽  
Vol 31 (7) ◽  
pp. 620-624 ◽  
Author(s):  
M. Elisabetta Guerzoni ◽  
Paolo Lambertini ◽  
Agostino Cavazza ◽  
Rosa Marchetti
Keyword(s):  
Carbon Source ◽  
Lipid Composition ◽  
Lipid Accumulation ◽  
Nitrogen Limitation ◽  
Culture Medium ◽  
Lipomyces Starkeyi ◽  
Cell Lipid ◽  
Branched Hydrocarbons ◽  
Lipid Fractions

Different cell lipid accumulation and composition patterns were observed in the yeasts Lipomyces starkeyi and Candida steatolytica by varying the nutritive conditions. In Lipomyces starkeyi 303 which accumulates high levels of lipids as triglycerides, the composition of the different lipid fractions was relatively unaffected by the culture medium. In contrast, the lipid composition of Candida steatolytica 20C was strongly affected by the composition of the medium under conditions of nitrogen limitation and when starch was used as the carbon source, high levels of straight and branched hydrocarbons were observed. [Traduction de l'auteur]

scholarly journals Production of thermostable extracellular α-amylase by a moderate thermophilic Bacillus licheniformis isolated from Qinarje Hot Spring (Ardebil prov. of Iran)

2017 ◽  
Vol 118 (4) ◽  
Author(s):  
Ali Deljou ◽  
Iman Arezi
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Bacillus Licheniformis ◽  
Bacterial Growth ◽  
Enzyme Production ◽  
Hot Spring ◽  
Basal Medium ◽  
Thermophilic Bacterium ◽  
Inoculum Size ◽  
Amylase Production

Background and Purpose: Amylases are most important industrial enzymes that account for about 30% of the world’s food, feed, fermentation, textile, detergent and cellulosic industries. This study aimed at optimum production of thermostable α-amylase via moderate thermophilic bacterium (Bacillus licheniformis) which was recently isolated from Qinarje Hot spring.Material and Methods: Initially, ability of bacterium for amylase activity was determined by starch hydrolysis test using Gram’s iodine staining. Then bacterial growth pattern and amylase production curves in basal production medium were graphically determined at different time intervals. Finally, effect of different temperature, pH, carbon source, nitrogen source, minerals and inoculum size were studied on bacterial growth and amylase production using turbidimetric and DNS method, respectively.Results: Optimum enzyme production achieved after 84 hours of inoculation from cultures growing at 40 ˚C and pH 9.0 in a medium containing 0.03% (w/v) of CaCl2, compared to the basal medium, results showed that the best enzyme production happened with inoculum size of 4% (v/v). The addition of 1% (w/v) rice husk (as a Carbon source) enhanced enzyme productivity up to 160% and substitution of the peptone and yeast extract with 1% (w/v) of tryptone (as a Nitrogen source) increased the α-amylase production up to 160%.Conclusion: Our findings show that B. licheniformis-AZ2 strain has an ability to produce the thermostable α-amylase which is suitable in starch processing and food industries. To be commercialized, further investigation is required for enhancement of the enzyme production.Keywords: Bacillus licheniformis; Optimization; Basal medium; Agricultural by-products.

scholarly journals Improved Laccase Production by Trametes pubescens MB89 in Distillery Wastewaters

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
P. J. Strong
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Culture Medium ◽  
Glucose Repression ◽  
Laccase Production ◽  
Copper Addition ◽  
Single Addition ◽  
Complex Nitrogen Source ◽  
Multiple Factor ◽  
Synergistic Increase

Various culture parameters were optimised for laccase synthesis by Trametes pubescens MB89, including pH, carbon source, nitrogen source, lignocellulosic supplements, and reported inducers. Glucose, in conjunction with a complex nitrogen source at pH 5.0, resulted in the highest laccase yield. Adding ethanol, copper, or 2,5-xylidine prior to inoculation further improved laccase concentrations. The addition of 2,5-xylidine was further investigated with multiple additions applied at varying times. This novel application substantially improved laccase production when applied regularly from inoculation and during the growth phase, and also countered glucose repression of laccase synthesis. Single and multiple factor changes were studied in three distillery wastewaters and a wine lees. A synergistic increase in laccase synthesis was observed with the addition of glucose, copper, and 2,5-xylidine. Single addition of 2,5-xylidine proved most beneficial with distillery wastewaters, while copper addition was most beneficial when using the wine lees as a culture medium.

scholarly journals Screening of Culture Conditions for Production of Xylanase from Landfill Soil Bacteria

2019 ◽  
Vol 19 (2) ◽  
pp. 470 ◽  
Author(s):  
Siti Nor Amira Rosli ◽  
Rohaida Che Man ◽  
Nasratun Masngut
Keyword(s):  
Carbon Source ◽  
Moisture Content ◽  
Nitrogen Source ◽  
Incubation Period ◽  
Xylanase Activity ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Xylanase Production ◽  
Initial Ph

Culture conditions including initial pH media, incubation period, inoculum size, type of carbon source, type of nitrogen source and its concentration, which affect xylanase production were screened via the one-factor-at-a-time approach. The bacteria used in the production of xylanase was isolated from the landfill site at Sg. Ikan, Kuala Terengganu, Malaysia. Three characterizations of the landfill soil were investigated for their moisture content, ash content, and pH. The culture conditions range used in the experimental work were between 6–30 h for the incubation period, with initial pH between 5–9, inoculum size between 1–20% v/v, carbon, nitrogen sources, and nitrogen source concentration between 1–5% w/v. Xylanase activity was estimated using dinitrosalicylic acid (DNS) based on the release of xylose under standard assay conditions. The landfill soil was observed to have pH between pH 3.4–7.2 with a moisture content between 12.4–33.7% and ash ranged between 3.5–4.3%. Results showed that the highest xylanase activity within studied ranges was recorded at 25.91±0.0641 U/mL with 10% (v/v) inoculum size, 1% (w/v) xylose as sole carbon source, mixture of 1% (w/v) peptone and 0.25% (w/v) ammonium sulphate as nitrogen sources, which was carried out at initial pH of 8.0 for 24 h incubation.

scholarly journals Optimisation of Lactic Acid Fermentation from Cassava Peel by Lactobacillus casei (ATCC334)

2021 ◽  
pp. 29-42
Author(s):  
Rahmat Folashade Zakariyah ◽  
Micheal Oluwaseyi Ojo ◽  
Kamoldeen Abiodun Ajijolakewu ◽  
Kudirat Bolanle Saliu ◽  
Risikat Nike Ahmed ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Nitrogen Source ◽  
Substrate Concentration ◽  
Lactobacillus Casei ◽  
Inoculum Size ◽  
Lactic Acid Fermentation ◽  
Partial Purification ◽  
Acid Fermentation ◽  
Cassava Peel ◽  
Source Concentration

The demand for lactic acid is steadily increasing due to the desire of its bioproduction over chemical synthesis. The associated cost, however, is a significant hurdle. This study reports lactic acid fermentation by Lactobacillus casei ATCC334 from cassava peel. It investigates the effect of unhydrolysed cassava peels, acidic, alkali hydrolysates; fermenting pH; substrate concentration; nitrogen source concentration; duration; and inoculum size. An attempt at a cheaper purification and recovery protocol relative to those currently in use was similarly performed. Acidic hydrolysate yielded 10.53%, unhydrolysed substrate gave 4.80% with alkali hydrolysate yielding 4.75%. The highest LA yield was obtained at pH 6.0, 2.0% v/v inoculum size, 25% w/v substrate concentration, 5% nitrogen source concentration. A post-optimisation combination yielded 18.3% LA suggesting that one-factor-at-a-time may be unsuitable for optimisation studies involving cassava peel and L. casei ATCC334. FTIR spectra of product suggests effective partial purification. Hence, an improvement in the optimization strategy for production is recommended for subsequent study.

scholarly journals Phytotoxin produced by Bipolaris euphorbiae in-vitro is effective against the weed Euphorbia heterophylla

2002 ◽  
Vol 45 (2) ◽  
pp. 233-240 ◽  
Author(s):  
Aneli M. Barbosa ◽  
Cristina G. M. Souza ◽  
Robert F. H. Dekker ◽  
Rafael C. Fonseca ◽  
Dalva T. Ferreira
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Sole Carbon Source ◽  
Host Plants ◽  
Culture Medium ◽  
Virulent Strain ◽  
Fungal Strain ◽  
Malt Extract ◽  
Mato Grosso

Four virulent strain isolates of the fungus, Bipolaris euphorbiae (previously identified as a Helminthosporium sp.), isolated from host plants in four states within Brazil were screened for the production of phytotoxins that promoted wilting and defoliation of the Brazilian weed, Euphorbia heterophylla, commonly found growing among soyabean crops. Only one isolate, B. euphorbiae Strain I (EUPH petropar from Mato Grosso state), produced phytotoxin in-vitro when grown in stationary culture for 7 d at 28 ° C on minimum salts medium supplemented with 1.5 % glucose as the sole carbon source. Phytotoxin was also produced when the fungal strain was grown on fructose, galactose, mannose, xylose and sucrose. The addition of nitrogen source (yeast extract, peptone or malt extract) to the culture medium did not influence phytotoxin production. The phytotoxin produced by Strain I was most active at pH 6.0, stable between pH 3-9, and was highly thermostable, remaining fully active when heated at 90 ° C for 1 h.

The influence of the initial concentrations of glucose and yeast extract on the ethanolic fermentation by Pachysolen tannophilus

1990 ◽  
Vol 55 (3) ◽  
pp. 854-866 ◽  
Author(s):  
Rodríguez V. Bravo ◽  
Rubio F. Camacho ◽  
Villasclaras S. Sánchez ◽  
Vico M. Castro
Keyword(s):  
Cell Growth ◽  
Ethanol Production ◽  
Yeast Extract ◽  
Culture Medium ◽  
Ethanolic Fermentation ◽  
Pachysolen Tannophilus ◽  
Significant Component ◽  
Batch Cultures

The ethanolic fermentation in batch cultures of Pachysolen tannophilus was studied experimentally varying the initial concentrations of two of the components in the culture medium: glucose between 0 and 200 g l-1 and yeast extract between 0 and 8 g l-1. The yeast extract appears to be a significant component both in cell growth and for ethanol production.

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