scholarly journals Antibacterial Activity of Intracellular Greenly Fabricated Selenium Nanoparticle of Lactobacillus pentosus ADET MW861694 against Selected Food Pathogens

2021 ◽  
Vol 10 (1) ◽  
pp. 39-51
Author(s):  
Bukola Christianah Adebayo-Tayo ◽  
Bola Olawunmi Yusuf ◽  
Solomon Omoniyi Alao
2016 ◽  
Vol 64 (3) ◽  
pp. 289-300 ◽  
Author(s):  
Yigang Xu ◽  
Xiaolin Zong ◽  
Bing Han ◽  
Yijing Li ◽  
Lijie Tang

In this study, Lactobacillus pentosus expressing porcine lactoferrin (pLF) was tested for in vitro antibacterial activity and for its ability to enhance immunity induced by an orally administered Aujeszky’s disease virus (ADV) vaccine. The cDNA encoding N-terminus of pLF was cloned into a Lactobacillus-specific plasmid to produce L. pentosus pLF expressing transformants (pPG612.1-pLFN/ L. pentosus). The antimicrobial activity of the recombinant pLF protein inhibited bacterial growth in vitro. The supernatant of pPG612.1-pLF-N/L. pentosus had an inhibitory effect on Staphylococcus aureus strain CVCC26003, Bacillus subtilis strain CVCC63501, Escherichia coli strain CVCC10141 and Salmonella enterica ssp. entericaCholeraesuis strain CVCC79102, while it did not inhibit the growth of Lactobacillus casei strain ATCC393. A mouse model was established to test the effectiveness of the orally administered probiotic L. pentosus recombinant strain in the gastrointestinal tract. Mice were immunised with an attenuated porcine Aujeszky’s disease virus (ADV) vaccine. Serum antibody levels determined using a mouse Aujeszky’s disease IgG ELISA showed that IgG levels were significantly higher in the pPG612.1-pLFN/L. pentosus group than in the PBS and Lactobacillus pentosus groups at days 7 and 21 (P < 0.01) and at day 14 (P < 0.05), indicating that this oral recombinant strain can improve the effectiveness of the vaccine and play a role in immune enhancement through humoral immunity. These results suggest that the recombinant Lactobacillus pentosus not only has the beneficial characteristics of lactic acid bacteria but also produces biologically functional lactoferrin.


2018 ◽  
Vol 40 (1) ◽  
pp. 36674
Author(s):  
Marcela Mona Sá Santos ◽  
Filipe Miguel Pereira da Silva ◽  
Juliana Fonseca Moreira da Silva ◽  
Raphael Sanzio Pimenta

2021 ◽  
pp. 1-9
Author(s):  
Roya Saddat Ghaderi ◽  
Fatemeh Adibian ◽  
Zahra Sabouri ◽  
Javid Davoodi ◽  
Monireh Kazemi ◽  
...  

Author(s):  
Anushya P ◽  
Preetha S ◽  
Lavanya Prathap ◽  
Jeevitha M

Nanotechnology has become one among the promising approaches for innovations which fulfil the human needs. Nanoparticles even have many applications in several fields like nanocomposites, medical imaging, nanobiocomposite filters, targeted drug delivery and hyperthermia of tumours. In which Selenium is an important micronutrient for living organisms. These nanoparticles are safe, eco friendly, inexpensive and nontoxic. Enterococcus faecalis  is an emergent gram - positive opportunistic pathogen that is the causative agent of several nosocomial infections and surgical wound infections. Therefore, it is becoming increasingly necessary to find other alternative treatments than commonly utilized drugs. The purpose of this study is to assess the antibacterial activity of Capparis decidua  fruit mediated selenium nanoparticles( cds-se Nps ) against Enterococcus faecalis. In this experimental study Se Nps were prepared by the reaction of 30mM sodium selenite solution and extracts of Capparis decidua. Antibacterial activity of SeNPs was assessed by using a disc diffusion method against Enterococcus faecalis. The SeNPs were characterized by UV-visible spectrophotometers. In the present study, the zone of inhibition shows 32mm, 35 mm, 37mm and 30 mm at concentration of 50 microliter, 100 microliter, 150 microliter and antibody respectively. The Capparis decidua  fruit mediated SeNp showed a good antibacterial activity against the pathogen Enterococcus faecalis 


2013 ◽  
Vol 97 (24) ◽  
pp. 10321-10328 ◽  
Author(s):  
Layal Karam ◽  
Charafeddine Jama ◽  
Anne-Sophie Mamede ◽  
Samir Boukla ◽  
Pascal Dhulster ◽  
...  

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