Glycosmis pentaphylla (Retz.) DC leaf extract mediated synthesis of selenium nanoparticle and investigation of its antibacterial activity against urinary tract pathogens

2021 ◽  
pp. 100894
Author(s):  
Rajesh Dev Sarkar ◽  
Priyanka Lahkar ◽  
Mohan Chandra Kalita
Keyword(s):  
Antibacterial Activity ◽  
Urinary Tract ◽  
Leaf Extract ◽  
Selenium Nanoparticle

scholarly journals IN VITRO ANTIBACTERIAL EFFICACY OF PLECTRANTHUS AMBOINICUS MEDIATED SILVER NANOPARTICLES AGAINST URINARY TRACT PATHOGENS

2019 ◽  
pp. 153-159
Author(s):  
SREELAKSHMY S ◽  
THANGAPANDIYAN S
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Urinary Tract ◽  
Leaf Extract ◽  
Spherical Shape ◽  
Plate Count ◽  
Dilution Method ◽  
Klebsiella Pneumonia ◽  
Plectranthus Amboinicus

Objective: The objective of the present study is to synthesize green mediated silver nanoparticles (AgNPs) using Plectranthus amboinicus leaf extract and to evaluate its antibacterial activity against selected urinary tract infection (UTI) causing bacteria. Methods: Phytoconstituents present in leaf extract of P. amboinicus (Lour.) Spreng were analyzed by standard qualitative tests. The synthesized AgNPs were characterized by visual inspection, ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and energy dispersive spectroscopy. The antibacterial activity of synthesized AgNPs was examined against the UTI pathogens such as Pseudomonas aeruginosa, Enterobacter sp., Klebsiella pneumonia, and Escherichia coli. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgNPs were carried out by broth dilution method and standard plate count method, respectively. Results: Synthesized AgNPs exhibited a spherical shape with an average size of 17.3 nm. The antibacterial activity of AgNPs against tested bacterial pathogens showed a maximum inhibition zone of 22.00±1.00 mm against P. aeruginosa. The bacterial growth inhibition was confirmed by MIC and MBC. Conclusion: P. amboinicus leaf extract mediated AgNPs could be used as an effective antibacterial agent for the management of UTIs. Surface modifications of urinary catheters with AgNPs may prevent the risk of contamination and the associated infections.

Evaluation of antioxidant enzyme content, phenolic content, and antibacterial activity of Commiphora gileadensis grown in Saudi Arabia

2021 ◽  
Vol 19 (4) ◽  
pp. 329-343
Author(s):  
Yaaser Q. Almulaiky ◽  
Ammar AL-Farga
Keyword(s):  
Antibacterial Activity ◽  
Saudi Arabia ◽  
Oral Hygiene ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Reference Drug ◽  
Wide Range ◽  
Peel Extract

Commiphora gileadensis is commonly used in Saudi Arabia for oral hygiene. A lack of data about its biological activity encouraged us to evaluate the antioxidant and antibacterial activities of its leaf and stem extracts. Ethanol, methanol, acetone and deionized water were tested as extraction solvents. 80% methanol gave the highest extracted concentrations of phenolic and flavonoid substances. The leaf and stem extracts were respectively evaluated for their radical scavenging activity with DPPH (EC50 = 3.39, and 1.06), ABTS (EC50 = 0.690, and 0.55), and peroxide scavenging activity (EC50 = 2.43, and 1.28). GC-MS identified a wide range of compounds that may be responsible for these activities of the results observed. The highest levels of chlorophyll, carotenoids, and lycopene were found in the leaf extract while level of proanthocyanidins was found in the stem peels extract. The peroxidase and catalase activities of stem peel extract were higher than those of the leaf extract. The findings showed that the leaf and stem peel extracts of C. gileadensis exhibited significant antibacterial activity against the test organisms. The minimum inhibitory concentrations for the plant extracts were compared with the standard reference drug Augmentin but the time–kill curves for the C. gileadensis extracts showed that they were less effective than Augmentin. Moreover, the stem peel extract exhibited stronger antibacterial activity than the leaf extract. In conclusion, C. gileadensis can be an important source of natural antioxidants, used as a healthy chewing stick for teeth brushing and oral hygiene purposes.

scholarly journals The Antibacterial Activity of Human Amniotic Membrane against Multidrug-Resistant Bacteria Associated with Urinary Tract Infections: New Insights from Normal and Cancerous Urothelial Models

Biomedicines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 218
Author(s):  
Taja Železnik Ramuta ◽  
Larisa Tratnjek ◽  
Aleksandar Janev ◽  
Katja Seme ◽  
Marjanca Starčič Erjavec ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Amniotic Membrane ◽  
Multidrug Resistant ◽  
Basic Knowledge ◽  
Resistant Bacteria ◽  
Human Amniotic Membrane ◽  
Multidrug Resistant Bacteria ◽  
Tract Infections

Urinary tract infections (UTIs) represent a serious global health issue, especially due to emerging multidrug-resistant UTI-causing bacteria. Recently, we showed that the human amniotic membrane (hAM) could be a candidate for treatments and prevention of UPEC and Staphylococcus aureus infections. However, its role against multidrug-resistant bacteria, namely methicillin-resistant S. aureus (MRSA), extended-spectrum beta-lactamases (ESBL) producing Escherichia coli and Klebsiella pneumoniae, vancomycin-resistant Enterococci (VRE), carbapenem-resistant Acinetobacter baumannii, and Pseudomonas aeruginosa has not yet been thoroughly explored. Here, we demonstrate for the first time that the hAM homogenate had antibacterial activity against 7 out of 11 tested multidrug-resistant strains, the greatest effect was on MRSA. Using novel approaches, its activity against MRSA was further evaluated in a complex microenvironment of normal and cancerous urinary bladder urothelia. Even short-term incubation in hAM homogenate significantly decreased the number of bacteria in MRSA-infected urothelial models, while it did not affect the viability, number, and ultrastructure of urothelial cells. The hAM patches had no antibacterial activity against any of the tested strains, which further exposes the importance of the hAM preparation. Our study substantially contributes to basic knowledge on the antibacterial activity of hAM and reveals its potential to be used as an antibacterial agent against multidrug-resistant bacteria.

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