scholarly journals Strain Selection and Optimization of Mixed Culture Conditions for Lactobacillus pentosus K1-23 with Antibacterial Activity and Aureobasidium pullulans NRRL 58012 Producing Immune-Enhancing ��-Glucan

2018 ◽  
Vol 28 (5) ◽  
pp. 697-706 ◽  
Author(s):  
Ashokkumar Sekar ◽  
Myoungjin Kim ◽  
Hyeong Chul Jeong ◽  
Keun Kim
Keyword(s):  
Antibacterial Activity ◽  
Mixed Culture ◽  
Aureobasidium Pullulans ◽  
Culture Conditions ◽  
Strain Selection ◽  
Lactobacillus Pentosus

scholarly journals Streptomyces: isolation, optimization of culture conditions and extraction of secondary metabolites

2016 ◽  
Vol 5 (3) ◽  
pp. 27-32 ◽  
Author(s):  
Ahmed I. Khattab ◽  
Eltahir H. Babiker ◽  
Humodi A. Saeed
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Pharmaceutical Journal ◽  
Growth Conditions ◽  
Culture Conditions ◽  
Physical Culture ◽  
Indicator Organisms ◽  
Streptomyces Spp ◽  
Promising Source ◽  
Soil Sediments

The objectives of this study were to isolate and identify Streptomyces from soil sediments as well as to optimize cultural growth conditions for maximum antibacterial productivity. A total of fifty soil sediments were collected from Red Sea, Sudan. The soil sediments were pretreated and cultivated on agar medium. Promising Streptomyces spp. were isolated by agar overlay method using indicator organisms. Optimization of chemical and physical culture conditions was carried out. The later was judged by assessment of antibacterial activity. Ethyl acetate was used to extract the secondary metabolite compounds. The separation of the active ingredients was performed using both thin layer chromatography (TLC) and gas chromatography-mass spectrometer (GC-MS). The results revealed nine strains of Streptomyces. Of them two (PS1 and PS28) isolates exhibited high activity against pathogenic bacteria. The optimum growth conditions were pH 7.5, temperature at 30°C, soyabean concentration 2.5 g/l, incubation period in 7 days, MgSO4.7H2O conc. 1g/l and K2HPO4 conc. 2.5g/l. TLC test showed three and two fragments from metabolites of PS1 and PS28 respectively, while the GC-MS analysis revealed eight and eleven compounds with antibacterial activity of PS1 and PS28 respectively. It is concluded that marine is promising source of secondary metabolites.Khattab et al., International Current Pharmaceutical Journal, February 2016, 5(3): 27-32

scholarly journals Effects of Culture Conditions on the Antibacterial Activity of Streptomyces Spp. against Erwinia Spp. Causing Soft Rot Disease on Asparagus Officinalis

2021 ◽  
Vol 4 (2) ◽  
pp. 1077-1086
Author(s):  
Nguyen Thanh Trung ◽  
Nguyen Thi Van Anh ◽  
Tran Thi Dao ◽  
Nguyen Thanh Huyen ◽  
Pham Le Anh Minh ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ornamental Plants ◽  
Soft Rot ◽  
Antibacterial Activities ◽  
Culture Conditions ◽  
Asparagus Officinalis ◽  
Effective Control ◽  
Soft Rot Disease ◽  
Streptomyces Spp ◽  
Rot Disease

Erwinia is a genus of Enterobacteriacea containing mostly pathogens, which cause soft rot disease in many ornamental plants and crops, including Asparagus officinalis. Chemical treatments to control Erwinia have lost their attractiveness because of the development of resistant strains and the negative impacts on the environment and human health. Therefore, the study of biological controls of soft rot disease has gained great importance. There are several types of microorganisms that show activity against Erwinia spp. such as Pseudomonas fluorescence, Bacillus subtilis, and Streptomyces spp. Among them, Streptomyces spp. are found to be the most effective control agents. In this study, 64 isolates of Streptomyces were screened for their antibacterial activity against Erwinia spp. The results indicated that 18 isolates showed an antagonistic reaction against Erwinia spp. Among them, isolate D5.1 showed the highest inhibition activity. In addition, the morphological and antibacterial activities of isolate D5.1 grown in different conditions were also characterized. 

scholarly journals Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed Culture and Elicitation Conditions for the Production of Antimicrobial Compounds

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 673
Author(s):  
Kattia Núñez-Montero ◽  
Damián Quezada-Solís ◽  
Zeinab G. Khalil ◽  
Robert J. Capon ◽  
Fernando D. Andreote ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Culture Media ◽  
Genome Mining ◽  
Gene Clusters ◽  
Culture Conditions ◽  
Bioactive Metabolites ◽  
Bacterial Strains ◽  
Antarctic Bacteria ◽  
New Antibiotics

Concern about finding new antibiotics against drug-resistant pathogens is increasing every year. Antarctic bacteria have been proposed as an unexplored source of bioactive metabolites; however, most biosynthetic gene clusters (BGCs) producing secondary metabolites remain silent under common culture conditions. Our work aimed to characterize elicitation conditions for the production of antibacterial secondary metabolites from 34 Antarctic bacterial strains based on MS/MS metabolomics and genome mining approaches. Bacterial strains were cultivated under different nutrient and elicitation conditions, including the addition of lipopolysaccharide (LPS), sodium nitroprusside (SNP), and coculture. Metabolomes were obtained by HPLC-QTOF-MS/MS and analyzed through molecular networking. Antibacterial activity was determined, and seven strains were selected for genome sequencing and analysis. Biosynthesis pathways were activated by all the elicitation treatments, which varies among strains and dependents of culture media. Increased antibacterial activity was observed for a few strains and addition of LPS was related with inhibition of Gram-negative pathogens. Antibiotic BGCs were found for all selected strains and the expressions of putative actinomycin, carotenoids, and bacillibactin were characterized by comparison of genomic and metabolomic data. This work established the use of promising new elicitors for bioprospection of Antarctic bacteria and highlights the importance of new “-omics” comparative approaches for drug discovery.

Chemical and biological characterization of siderophore produced by the marine-derived Aureobasidium pullulans HN6.2 and its antibacterial activity

BioMetals ◽  
2009 ◽  
Vol 22 (6) ◽  
pp. 965-972 ◽  
Author(s):  
Weilin Wang ◽  
Zhe Chi ◽  
Guanglei Liu ◽  
Muhammad Aslam Buzdar ◽  
Zhenming Chi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Aureobasidium Pullulans ◽  
Biological Characterization

scholarly journals Lactobacillus pentosus expressing porcine lactoferrin elevates antibacterial activity and improves the efficacy of vaccination against Aujeszky’s disease

2016 ◽  
Vol 64 (3) ◽  
pp. 289-300 ◽  
Author(s):  
Yigang Xu ◽  
Xiaolin Zong ◽  
Bing Han ◽  
Yijing Li ◽  
Lijie Tang
Keyword(s):  
Antibacterial Activity ◽  
Disease Virus ◽  
Recombinant Strain ◽  
Serum Antibody ◽  
Subtilis Strain ◽  
Lactobacillus Pentosus ◽  
Aujeszky's Disease ◽  
Aujeszky’S Disease ◽  
Aujeszky’S Disease Virus

In this study, Lactobacillus pentosus expressing porcine lactoferrin (pLF) was tested for in vitro antibacterial activity and for its ability to enhance immunity induced by an orally administered Aujeszky’s disease virus (ADV) vaccine. The cDNA encoding N-terminus of pLF was cloned into a Lactobacillus-specific plasmid to produce L. pentosus pLF expressing transformants (pPG612.1-pLFN/ L. pentosus). The antimicrobial activity of the recombinant pLF protein inhibited bacterial growth in vitro. The supernatant of pPG612.1-pLF-N/L. pentosus had an inhibitory effect on Staphylococcus aureus strain CVCC26003, Bacillus subtilis strain CVCC63501, Escherichia coli strain CVCC10141 and Salmonella enterica ssp. entericaCholeraesuis strain CVCC79102, while it did not inhibit the growth of Lactobacillus casei strain ATCC393. A mouse model was established to test the effectiveness of the orally administered probiotic L. pentosus recombinant strain in the gastrointestinal tract. Mice were immunised with an attenuated porcine Aujeszky’s disease virus (ADV) vaccine. Serum antibody levels determined using a mouse Aujeszky’s disease IgG ELISA showed that IgG levels were significantly higher in the pPG612.1-pLFN/L. pentosus group than in the PBS and Lactobacillus pentosus groups at days 7 and 21 (P < 0.01) and at day 14 (P < 0.05), indicating that this oral recombinant strain can improve the effectiveness of the vaccine and play a role in immune enhancement through humoral immunity. These results suggest that the recombinant Lactobacillus pentosus not only has the beneficial characteristics of lactic acid bacteria but also produces biologically functional lactoferrin.

scholarly journals Liamocin oil from Aureobasidium pullulans has antibacterial activity with specificity for species of Streptococcus

2015 ◽  
Vol 68 (10) ◽  
pp. 642-645 ◽  
Author(s):  
Kenneth M Bischoff ◽  
Timothy D Leathers ◽  
Neil PJ Price ◽  
Pennapa Manitchotpisit
Keyword(s):  
Antibacterial Activity ◽  
Aureobasidium Pullulans
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