scholarly journals Therapeutic potential of bleomycin plus suicide or interferon-β gene transfer combination for spontaneous feline and canine melanoma

Oncoscience ◽  
2017 ◽  
Vol 4 (11-12) ◽  
pp. 199-214 ◽  
Author(s):  
Lucrecia Agnetti ◽  
Chiara Fondello ◽  
Marcela S. Villaverde ◽  
Gerardo C. Glikin ◽  
Liliana M.E. Finocchiaro
Keyword(s):  
Gene Transfer ◽  
Therapeutic Potential ◽  
Interferon Β ◽  
Canine Melanoma

scholarly journals Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation

2020 ◽  
Vol 11 ◽  
Author(s):  
Otto Luiz Dutra Cerqueira ◽  
Maria Alejandra Clavijo-Salomon ◽  
Elaine Cristina Cardoso ◽  
Tharcisio Citrangulo Tortelli Junior ◽  
Samir Andrade Mendonça ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Cell Line ◽  
Melanoma Cell ◽  
Immune Activation ◽  
Melanoma Cell Line ◽  
Human Melanoma ◽  
Human Melanoma Cell Line ◽  
Human Melanoma Cell ◽  
Interferon Β

scholarly journals Therapeutic Potential of Gene Transfer to Testis; Myth or Reality?

10.5772/20488 ◽  
2011 ◽  
Author(s):  
Yoshiyuki Kojima ◽  
Kentaro Mizuno ◽  
Yukihiro Umemoto ◽  
Shoichi Sasaki ◽  
Yutaro Hayashi ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Therapeutic Potential

Abstract 2103: Prevention of Pulmonary Hypertension by ACE2 Gene Transfer to Lungs

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Yoriko Yamazato ◽  
KwonHo Hong ◽  
Dae Song Jang ◽  
Anderson J Ferreira ◽  
Masanobu Yamazato ◽  
...  
Keyword(s):  
Pulmonary Hypertension ◽  
Gene Transfer ◽  
Wall Thickness ◽  
Lentiviral Vector ◽  
Therapeutic Potential ◽  
Systolic Pressure ◽  
Vector Particle ◽  
Mrna Levels ◽  
Alveolar Cells ◽  
Ace2 Gene

Pulmonary hypertension (PH) is characterized by increase in pulmonary vascular resistance, and narrowing and loss of pulmonary microvasculare. There is an indispensable need to develop innovative approaches for its control since PH becomes refractory to current therapies in later stages. Recent discovery of angiotensin converting enzyme 2 (ACE2), its involvement in cardiac remodeling, coupled with the limited success of ACE inhibitors in PH has led us to hypothesize that shifting the balance of renin-angiotensin system (RAS) to vasoprotective ACE2-Ang1–7- mas receptor axis would result the beneficial outcome in PH. We tested this hypothesis with the use of ACE2 overexpression in lungs by lentiviral vector-mediated gene transfer. Lentiviral vector particle(3x10^8 TU) containing murine ACE2 (letni-ACE2) were injected into 6 weeks old C57BL/6 mice prior to induction of PH by administration of weekly 600 mg/kg of monocrotaline (MCT) for 8 weeks for prevention studies. In addition, lenti-ACE2 was delivered following 6 weeks MCT treatment in reversal studies. Right ventricle systolic pressure (RVSP), Real-time RT-PCR, immunohisitochemistory of ACE2 and Ang (1–7) and histology of lungs in control and lent-ACE2 treated mice were carried out to evaluated the outcome on PH. Delivery of lenti-ACE2 resulted in a long-term increase in ACE2 expression in the lungs. A 60% and 100 % increases in protein and mRNA levels for ACE2 were observed. ACE2 and Ang (1–7) immunoreactivity were observed in epithelial and alveolar cells and alveolar macrophages. MCT treatment increased in RVSP (MCT 44.5+/−5.7 mmHg, control 24+/−1.0mmHg), RV hypertrophy (RV/LV+Sp ratio; 0.31+/−0.01), and wall thickness of pulmonary vessels. ACE2 gene transfer prevented increases in RVSP (26.1+/− 1.1mmHg), and RV hypertrophy (0.26+/−0.1), and reduced vessel wall thickness. In addition, ACE2 overexpression resulted in a significant reversal of RVSP (23.5+/−0.6mmHg). Futhermore, ACE2 overexpression in mice exhibited better general appearance and gained weight compared to MCT-treated mice. ACE2 gene transfer to lungs prevents and reverses vascular remodeling and PH in MCT model of PH. These observations suggest that targeting of pulmonary ACE2 holds novel therapeutic potential for PH.

Adrenomedullin: angiogenesis and gene therapy

2005 ◽  
Vol 288 (6) ◽  
pp. R1432-R1437 ◽  
Author(s):  
Noritoshi Nagaya ◽  
Hidezo Mori ◽  
Shinsuke Murakami ◽  
Kenji Kangawa ◽  
Soichiro Kitamura
Keyword(s):  
Gene Transfer ◽  
Cell Biology ◽  
Therapeutic Potential ◽  
Lattice Structure ◽  
Rabbit Model ◽  
Mitogen Activated Protein Kinase ◽  
Tissue Ischemia ◽  
Hypoxic Conditions

Adrenomedullin (AM) is a potent, long-lasting vasodilator peptide that was originally isolated from human pheochromocytoma. AM signaling is of particular significance in endothelial cell biology since the peptide protects cells from apoptosis, promotes angiogenesis, and affects vascular tone and permeability. The angiogenic effect of AM is mediated by activation of Akt, mitogen-activated protein kinase/extracellular signal-regulated kinase 1/2, and focal adhesion kinase in endothelial cells. Both AM and its receptor, calcitonin receptor-like receptor, are upregulated through a hypoxia-inducible factor-1-dependent pathway under hypoxic conditions. Thus AM signaling plays an important role in the regulation of angiogenesis in hypoxic conditions. Recently, we have developed a nonviral vector, gelatin. Positively charged gelatin holds negatively charged plasmid DNA in its lattice structure. DNA-gelatin complexes can delay gene degradation, leading to efficient gene transfer. Administration of AM DNA-gelatin complexes induces potent angiogenic effects in a rabbit model of hindlimb ischemia. Thus gelatin-mediated AM gene transfer may be a new therapeutic strategy for the treatment of tissue ischemia. Endothelial progenitor cells (EPCs) play an important role in endothelial regeneration. Interestingly, EPCs phagocytose ionically linked DNA-gelatin complexes in coculture, which allows nonviral gene transfer into EPCs. AM gene transfer into EPCs inhibits cell apoptosis and induces proliferation and migration, suggesting that AM gene transfer strengthens the therapeutic potential of EPCs. Intravenous administration of AM gene-modified EPCs regenerate pulmonary endothelium, resulting in improvement of pulmonary hypertension. These results suggest that in vivo and in vitro transfer of AM gene using gelatin may be applicable for intractable cardiovascular disease.

scholarly journals A Phase I Trial of Repeated Intrapleural Adenoviral-mediated Interferon-β Gene Transfer for Mesothelioma and Metastatic Pleural Effusions

2010 ◽  
Vol 18 (4) ◽  
pp. 852-860 ◽  
Author(s):  
Daniel H Sterman ◽  
Adri Recio ◽  
Andrew R Haas ◽  
Anil Vachani ◽  
Sharyn I Katz ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Phase I ◽  
Phase I Trial ◽  
Pleural Effusions ◽  
Interferon Β

Interferon-β gene transfer induces a strong cytotoxic bystander effect on melanoma cells

2015 ◽  
Vol 72 ◽  
pp. 44-51 ◽  
Author(s):  
Úrsula A. Rossi ◽  
María L. Gil-Cardeza ◽  
Marcela S. Villaverde ◽  
Liliana M.E. Finocchiaro ◽  
Gerardo C. Glikin
Keyword(s):  
Gene Transfer ◽  
Bystander Effect ◽  
Melanoma Cells ◽  
Interferon Β

scholarly journals Cytokine-Enhanced Vaccine and Interferon-β plus Suicide Gene Therapy as Surgery Adjuvant Treatments for Spontaneous Canine Melanoma

2015 ◽  
Vol 26 (6) ◽  
pp. 367-376 ◽  
Author(s):  
Liliana M.E. Finocchiaro ◽  
Chiara Fondello ◽  
María L. Gil-Cardeza ◽  
Úrsula A. Rossi ◽  
Marcela S. Villaverde ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Suicide Gene ◽  
Suicide Gene Therapy ◽  
Interferon Β ◽  
Canine Melanoma
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