BMPR2 promotes invasion and metastasis via the RhoA-ROCK-LIMK2 pathway in human osteosarcoma cells

Background/Aims: Osteosarcoma (OS) is the most common primary malignant tumor of bone tissue. Although treatment effectiveness has improved, the OS survival rate has fluctuated in recent years. Andrographolide (AG) has been reported to have antitumor activity against a variety of tumors. Our aim was to investigate the effects and potential mechanisms of AG in human osteosarcoma. Methods: Cell viability and morphological changes were assessed by MTT and live/dead assays. Apoptosis was detected using Annexin V-FITC/PI double staining, DAPI, and caspase-3 assays. Autophagy was detected with mRFP-GFP-LC3 adenovirus transfection and western blot. Cell migration and invasion were detected by wound healing assay and Transwell® experiments. Results: AG dose-dependently reduced the viability of osteosarcoma cells. No increase in apoptosis was detected in AG-treated human OS MG-63 and U-2OS cells, and the pan-caspase inhibitor z-VAD did not attenuate AG-induced cell death. However, AG induced autophagy by suppressing PI3K/Akt/mTOR and enhancing JNK signaling pathways. 3-MA and Beclin-1 siRNA could reverse the cytotoxic effects of AG. In addition, AG inhibited the invasion and metastasis of OS, and this effect could be reversed with Beclin-1 siRNA. Conclusion: AG inhibits viability and induces autophagic death in OS cells. AG-induced autophagy inhibits the invasion and metastasis of OS.

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Relationship between the expression of MTA1 gene and invasion and metastasis of human osteosarcoma cells

The Chinese-German Journal of Clinical Oncology ◽

10.1007/s10330-005-0416-3 ◽

2006 ◽

Vol 5 (3) ◽

pp. 200-203

Author(s):

Xinzhi Li ◽

Anmin Chen ◽

Chengla Yi ◽

Fengjin Guo ◽

Zhenqiang Luo ◽

...

Keyword(s):

Osteosarcoma Cells ◽

Invasion And Metastasis ◽

Human Osteosarcoma ◽

Human Osteosarcoma Cells

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Transfection and overexpression of 11-ß-hydroxysteroid kinase type 1 in human osteosarcoma cells (HOS 58) and primary human osteoblasts

Experimental and Clinical Endocrinology & Diabetes ◽

10.1055/s-2006-932987 ◽

2006 ◽

Vol 114 (S 1) ◽

Author(s):

PJ Braun ◽

V Ritz ◽

V Bähr ◽

S Diederich ◽

M Hüfner ◽

...

Keyword(s):

Osteosarcoma Cells ◽

Human Osteoblasts ◽

Human Osteosarcoma ◽

Human Osteosarcoma Cells ◽

Primary Human Osteoblasts

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Antiproliferative properties of oleuropein in human osteosarcoma cells

Bone Abstracts ◽

10.1530/boneabs.5.p109 ◽

2016 ◽

Author(s):

Jose M Moran ◽

Olga Leal-Hernandez ◽

Maria L Canal-Macias ◽

Jesus M Lavado-Garcia ◽

Raul Roncero-Martin ◽

...

Keyword(s):

Osteosarcoma Cells ◽

Human Osteosarcoma ◽

Human Osteosarcoma Cells

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Copper(II) Complexes with Saccharinate and Glutamine as Antitumor Agents: Cytoand Genotoxicity in Human Osteosarcoma Cells

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520616666160513130204 ◽

2017 ◽

Vol 17 (3) ◽

pp. 424-433 ◽

Cited By ~ 3

Author(s):

Juan Cadavid-Vargas ◽

Ignacio Leon ◽

Susana Etcheverry ◽

Eduardo Santi ◽

Maria Torre ◽

...

Keyword(s):

Antitumor Agents ◽

Osteosarcoma Cells ◽

Human Osteosarcoma ◽

Human Osteosarcoma Cells

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Assessment of the Nutraceutical Effects of Oleuropein and the Cytotoxic Effects of Adriamycin, When Administered Alone and in Combination, in MG-63 Human Osteosarcoma Cells

Nutrients ◽

10.3390/nu13020354 ◽

2021 ◽

Vol 13 (2) ◽

pp. 354

Author(s):

Katerina Gioti ◽

Anastasia Papachristodoulou ◽

Dimitra Benaki ◽

Nektarios Aligiannis ◽

Alexios-Leandros Skaltsounis ◽

...

Keyword(s):

Chemotherapeutic Agent ◽

Molecular Techniques ◽

Osteosarcoma Cells ◽

Elisa Method ◽

Chain Reaction ◽

Cytotoxic Effects ◽

Human Osteosarcoma ◽

Human Osteosarcoma Cells ◽

Anti Cancer ◽

Polymerase Chain

Oleuropein (OLEU) is the most distinguished phenolic compound found in olive fruit and the leaves of Olea europaea L., with several pharmacological properties, including anti-cancer actions. Adriamycin (ADR) is an anthracycline widely used as a chemotherapeutic agent, although it presents significant side effects. The aim of the present study was to investigate the effect of oleuropein alone (20 μg/mL) and in co-treatment with ADR (50 nM), in MG-63 human osteosarcoma cells. Therefore, cellular and molecular techniques, such as MTT assay, flow cytometry, real-time Polymerase Chain Reaction (PCR), western blot and Elisa method, as well as Nuclear Magnetic Resonance (NMR) spectroscopy, were applied to unveil changes in the signal transduction pathways involved in osteosarcoma cells survival. The observed alterations in gene, protein and metabolite levels denote that OLEU not only inhibits MG-63 cells proliferation and potentiates ADR’s cytotoxicity, but also exerts its action, at least in part, through the induction of autophagy.

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