scholarly journals Characterization of the effects of defined, multidimensional culture conditions on conditionally reprogrammed primary human prostate cells

Oncotarget ◽  
2017 ◽  
Vol 9 (2) ◽  
pp. 2193-2207 ◽  
Author(s):  
Lucas Tricoli ◽  
Aisha Naeem ◽  
Erika Parasido ◽  
John P. Mikhaiel ◽  
Muhammad Umer Choudhry ◽  
...  
Keyword(s):  
Culture Conditions ◽  
Human Prostate ◽  
Prostate Cells

scholarly journals Insights into the Functionality of the Putative Residues Involved in Enterocin AS-48 Maturation

2010 ◽  
Vol 76 (21) ◽  
pp. 7268-7276 ◽  
Author(s):  
Rubén Cebrián ◽  
Mercedes Maqueda ◽  
José Luis Neira ◽  
Eva Valdivia ◽  
Manuel Martínez-Bueno ◽  
...  
Keyword(s):  
Posttranslational Modifications ◽  
High Efficiency ◽  
Specific Protein ◽  
Culture Conditions ◽  
Site Directed Mutagenesis ◽  
Circular Structure ◽  
Cleavage Enzyme ◽  
Site Recognition ◽  
Biosynthetic Machinery

ABSTRACT AS-48 is a 70-residue, α-helical, cationic bacteriocin produced by Enterococcus faecalis and is very singular in its circular structure and its broad antibacterial spectrum. The AS-48 preprotein consists of an N-terminal signal peptide (SP) (35 residues) followed by a proprotein moiety that undergoes posttranslational modifications to yield the mature and active circular protein. For the study of the specificity of the region of AS-48 that is responsible for maturation, three single mutants have been generated by site-directed mutagenesis in the as-48A structural gene. The substitutions were made just in the residues that are thought to constitute a recognition site for the SP cleavage enzyme (His-1, Met1) and in those involved in circularization (Met1, Trp70). Each derivative was expressed in the enterococcal JH2-2 strain containing the necessary native biosynthetic machinery for enterocin production. The importance of these derivatives in AS-48 processing has been evaluated on the basis of the production and structural characterization of the corresponding derivatives. Notably, only two of them (Trp70Ala and Met1Ala derivatives) could be purified in different forms and amounts and are characterized for their bactericidal activity and secondary structure. We could not detect any production of AS-48 in JH2-2(pAM401-81 His-1Ile ) by using the conventional chromatographic techniques, despite the high efficiency of the culture conditions applied to produce this enterocin. Our results underline the different important roles of the mutated residues in (i) the elimination of the SP, (ii) the production levels and antibacterial activity of the mature proteins, and (iii) protein circularization. Moreover, our findings suggest that His-1 is critically involved in cleavage site recognition, its substitution being responsible for the blockage of processing, thereby hampering the production of the specific protein in the cellular culture supernatant.

scholarly journals 1044 Molecular characterization of radiation-induced transformation of human prostate epithelial cells

1995 ◽  
Vol 32 ◽  
pp. 245
Author(s):  
Michael Kuettel ◽  
Peter Thraves ◽  
Sarada Prasad ◽  
Susan Varghese ◽  
Anatoly Dritschilo
Keyword(s):  
Epithelial Cells ◽  
Molecular Characterization ◽  
Human Prostate ◽  
Prostate Epithelial Cells ◽  
Radiation Induced

Establishment and characterization of androgen-independent human prostate cancer cell lines, PcBra1, PcBra2, and PcBra3

2009 ◽  
Vol 46 (2) ◽  
pp. 123-130
Author(s):  
Camila B. Piantino ◽  
Juliana M. Sousa-Canavez ◽  
Marta Bellodi-Privato ◽  
Miguel Srougi ◽  
Luiz Heraldo Camara-Lopes ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Prostate Cancer Cell ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Human Prostate Cancer Cell ◽  
Prostate Cancer Cell Lines ◽  
Androgen Independent

Characterization of a novel Aspergillus niger beta-glucosidase tolerant to saccharification of lignocellulosic biomass products and fermentation inhibitors

2015 ◽  
Vol 69 (8) ◽  
Author(s):  
Alesandra Oriente ◽  
Robson Tramontina ◽  
Diandra de Andrades ◽  
Caroline Henn ◽  
Jose L. C. Silva ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Lignocellulosic Biomass ◽  
Culture Conditions ◽  
Industrial Wastes ◽  
Fermentation Inhibitors ◽  
State Culture ◽  
Solid State Culture ◽  
Atlantic Rainforest Biome ◽  
Beta Glucosidase

AbstractProperties of beta-glucosidase produced by Aspergillus niger URM 6642 recently isolated from the Atlantic rainforest biome and its potential tolerance to saccharification of lignocellulosic biomass products and fermentation inhibitors was evaluated. The fungus was cultivated under solid state culture conditions at 37°C with different agro-industrial wastes. High levels of beta-glucosidase (3778.9 U g

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