Characterization of a novel Aspergillus niger beta-glucosidase tolerant to saccharification of lignocellulosic biomass products and fermentation inhibitors

2015 ◽  
Vol 69 (8) ◽  
Author(s):  
Alesandra Oriente ◽  
Robson Tramontina ◽  
Diandra de Andrades ◽  
Caroline Henn ◽  
Jose L. C. Silva ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Lignocellulosic Biomass ◽  
Culture Conditions ◽  
Industrial Wastes ◽  
Fermentation Inhibitors ◽  
State Culture ◽  
Solid State Culture ◽  
Atlantic Rainforest Biome ◽  
Beta Glucosidase

AbstractProperties of beta-glucosidase produced by Aspergillus niger URM 6642 recently isolated from the Atlantic rainforest biome and its potential tolerance to saccharification of lignocellulosic biomass products and fermentation inhibitors was evaluated. The fungus was cultivated under solid state culture conditions at 37°C with different agro-industrial wastes. High levels of beta-glucosidase (3778.9 U g

scholarly journals Ellagic Acid Recovery by Solid State Fermentation of Pomegranate Wastes by Aspergillus niger and Saccharomyces cerevisiae: A Comparison

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3689 ◽  
Author(s):  
Federica Moccia ◽  
Adriana C. Flores-Gallegos ◽  
Mónica L. Chávez-González ◽  
Leonardo Sepúlveda ◽  
Stefania Marzorati ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Solid State ◽  
Aspergillus Niger ◽  
Ellagic Acid ◽  
Fold Increase ◽  
Industrial Applications ◽  
Solvent Ratio ◽  
State Culture ◽  
Acid Recovery ◽  
Solid State Culture

Fermentation in solid state culture (SSC) has been the focus of increasing interest because of its potential for industrial applications. In previous studies SSC of pomegranate wastes by Aspergillus niger has been extensively developed and optimized for the recovery of ellagic acid (EA), a high value bioactive. In this study we comparatively investigated the SSC of powdered pomegranate husks by A. niger and Saccharomyces cerevisiae and evaluated the recovery yields of EA by an ultrasound and microwave-assisted 7:3 water/ethanol extraction. Surprisingly enough, the yields obtained by S. cerevisiae fermentation (4% w/w) were found 5-fold higher than those of the A. niger fermented material, with a 10-fold increase with respect to the unfermented material. The EA origin was traced by HPLC analysis that showed a significant decrease in the levels of punicalagin isomers and granatin B and formation of punicalin following fermentation. Other extraction conditions that could warrant a complete solubilization of EA were evaluated. Using a 1:100 solid to solvent ratio and DMSO as the solvent, EA was obtained in 4% yields from S. cerevisiae fermented husks at a high purity degree. Hydrolytic treatment of S. cerevisiae fermented pomegranate husks afforded a material freed of the polysaccharides components that gave recovery yields of EA up to 12% w/w.

scholarly journals Optimization and characterization of a new lipopeptide biosurfactant produced by marine Brevibacterium aureum MSA13 in solid state culture

2010 ◽  
Vol 101 (7) ◽  
pp. 2389-2396 ◽  
Author(s):  
G. Seghal Kiran ◽  
T. Anto Thomas ◽  
Joseph Selvin ◽  
B. Sabarathnam ◽  
A.P. Lipton
Keyword(s):  
Solid State ◽  
State Culture ◽  
Solid State Culture ◽  
Lipopeptide Biosurfactant

Optimization of ellagic acid accumulation by Aspergillus niger GH1 in solid state culture using pomegranate shell powder as a support

2012 ◽  
Vol 47 (12) ◽  
pp. 2199-2203 ◽  
Author(s):  
L. Sepúlveda ◽  
A. Aguilera-Carbó ◽  
J.A. Ascacio-Valdés ◽  
R. Rodríguez-Herrera ◽  
J.L. Martínez-Hernández ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Ellagic Acid ◽  
State Culture ◽  
Solid State Culture ◽  
Acid Accumulation ◽  
Aspergillus Niger Gh1 ◽  
Shell Powder

scholarly journals Production and characterization of cellulases and hemicellulases from a consortium between Pleurotus ostreatus and Aspergillus niger cultured in agro-industrial wastes

2021 ◽  
Vol 10 (10) ◽  
pp. e396101019020
Author(s):  
Igor Magno Nicurgo Borges Rosa Martins ◽  
Luanna Stefanny Vieira Oliveira Gomes ◽  
Daniel Pasquini ◽  
Milla Alves Baffi
Keyword(s):  
Aspergillus Niger ◽  
Sugarcane Bagasse ◽  
Wheat Bran ◽  
Pleurotus Ostreatus ◽  
Temperature Effects ◽  
Microbial Growth ◽  
Industrial Wastes ◽  
Biomass Hydrolysis ◽  
Filter Paper Cellulase

The enzyme biosynthesis using agricultural wastes by solid state fermentation (SSF) and the study of their physicochemical properties are meaningful approaches to improve the biomass hydrolysis. Among them, β-glucosidases and β-xylosidases are key enzymes at the lignocellulose depolymerization, which act in the cleavage of oligosaccharides in monosaccharides. In this study, the production of hemicellulases and cellulases by Pleurotus ostreatus and Aspergillus niger monocultures or in consortium was investigated, using raw sugarcane bagasse (SB) and wheat bran (WB) as substrates. The highest enzymatic activities were observed in the crude extract produced by P. ostreatus PLO6 and A. niger SCBM4 consortium with 98.5, 62.9, 3.8, 12.4, 13.3 and 20.2 U/g for β-glucosidase (β-glu), β-xylosidase (Bxyl), filter paper cellulase (FPase), xylanase (Xyl), exoglucanase (Exgl) and endoglucanase (Engl), respectively. The pH and temperature effects on β-glu and β-xyl were characterized. Optimal activities were obtained at pH 4.0 and 45 °C for β-glu and 3.5 and 55 °C for β-xyl. Both enzymes were stable at acid pH and presented thermostability. The results indicated that the enzymatic cocktail demonstrated potential characteristics for future applications in saccharifications. The use of sugarcane bagasse and wheat bran for microbial growth contributed to aggregate value to these byproducts.

Recovery of pectolytic enzymes produced by solid state culture of Aspergillus niger

1999 ◽  
Vol 34 (2) ◽  
pp. 181-186 ◽  
Author(s):  
Leda R. Castilho ◽  
Tito L.M. Alves ◽  
Ricardo A. Medronho
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
State Culture ◽  
Solid State Culture ◽  
Pectolytic Enzymes
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