scholarly journals Evaluación del desarrollo y la estabilidad de una emulsión de vainilla (Vanilla planifolia) a partir de un extracto no alcohólico

Biotecnia ◽  
2021 ◽  
Vol 23 (3) ◽  
Author(s):  
Hugo Sergio Garcia Galindo
Keyword(s):  
Vanilla Planifolia

La vainilla (Vainilla planifolia) es considerada como el saborizante de mayor importancia en el mundo. Sin embargo, su aplicación en alimentos está limitada, ya que se comercializa principalmente como extractos etanólicos, debido a su naturaliza lipofílica. El objetivo de este trabajo fue elaborar nanoemulsiones de vainilla libre de alcohol, a partir de un extracto natural mediante homogenización por altas presiones, como estrategia para mejorar su solubilidad y estabilidad. El efecto de la presión y el número de ciclos se evaluaron mediante un diseño factorial de 23*3. Las emulsiones óptimas se almacenaron a 15, 25 y 35 ° C durante 42 días. A las emulsiones obtenidas se les evaluó tamaño de partícula e indice de estabilidad (TSI). La formulación óptima para generar una emulsión estable sin azúcar requirió 5 ciclos a una presión de 40 MPa, mientras que para la emulsión con azúcar fueron necesarios 9 ciclos a una presión de 20 MPa; con una composición de 17 % de glicerol. La emulsión se mantuvo estable a 15 y 25 º C durante 42 días. Los hallazgos de este estudio conducirán a la posibilidad de que esta formulación se utilice como uno de los métodos para mejorar su solubilidad y estabilidad

scholarly journals Shoots multiplication of vanilla (Vanilla planifolia) with benzyl amino purine and kinetin modification

2021 ◽  
Vol 672 (1) ◽  
pp. 012007
Author(s):  
D N Erawati ◽  
I Wardati ◽  
S Humaida ◽  
Y Mawadah ◽  
A Ikanafi’ah ◽  
...  
Keyword(s):  
Vanilla Planifolia ◽  
Benzyl Amino Purine

scholarly journals Asymbiotic germination of Vanilla planifolia in relation to the timing of seed collection and seed pretreatments

2021 ◽  
Vol 62 (1) ◽  
Author(s):  
Chih-Hsin Yeh ◽  
Kai-Yi Chen ◽  
Yung-I. Lee
Keyword(s):  
Seed Germination ◽  
Seed Development ◽  
Seed Coat ◽  
Germination Percentage ◽  
Vanilla Planifolia ◽  
Outermost Layer ◽  
Seed Collection ◽  
Seed Pretreatment ◽  
Immature Seeds ◽  
Mature Seeds

Abstract Background Vanilla planifolia is an important tropical orchid for production of natural vanilla flavor. Traditionally, V. planifolia is propagated by stem cuttings, which produces identical genotype that are sensitive to virulent pathogens. However, propagation with seed germination of V. planifolia is intricate and unstable because the seed coat is extremely hard with strong hydrophobic nature. A better understanding of seed development, especially the formation of impermeable seed coat would provide insights into seed propagation and conservation of genetic resources of Vanilla. Results We found that soaking mature seeds in 4% sodium hypochlorite solution from 75 to 90 min significantly increased germination. For the culture of immature seeds, the seed collection at 45 days after pollination (DAP) had the highest germination percentage. We then investigated the anatomical features during seed development that associated with the effect of seed pretreatment on raising seed germination percentage. The 45-DAP immature seeds have developed globular embryos and the thickened non-lignified cell wall at the outermost layer of the outer seed coat. Seeds at 60 DAP and subsequent stages germinated poorly. As the seed approached maturity, the cell wall of the outermost layer of the outer seed coat became lignified and finally compressed into a thick envelope at maturity. On toluidine blue O staining, the wall of outer seed coat stained greenish blue, indicating the presence of phenolic compounds. As well, on Nile red staining, a cuticular substance was detected in the surface wall of the embryo proper and the innermost wall of the inner seed coat. Conclusion We report a reliable protocol for seed pretreatment of mature seeds and for immature seeds culture based on a defined time schedule of V. plantifolia seed development. The window for successful germination of culturing immature seed was short. The quick accumulation of lignin, phenolics and/or phytomelanins in the seed coat may seriously inhibit seed germination after 45 DAP. As seeds matured, the thickened and lignified seed coat formed an impermeable envelope surrounding the embryo, which may play an important role in inducing dormancy. Further studies covering different maturity of green capsules are required to understand the optimal seed maturity and germination of seeds.

scholarly journals Evaluation of Chemical Variability of Cured Vanilla Beans (Vanilla tahitensis and Vanilla planifolia)

2009 ◽  
Vol 4 (10) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Christel Brunschwig ◽  
François Xavier Collard ◽  
Jean-Pierre Bianchini ◽  
Phila Raharivelomanana
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Dry Matter ◽  
Monounsaturated Fatty Acids ◽  
Vanilla Planifolia ◽  
Chemical Variability ◽  
Total Fatty Acids ◽  
Curing Method ◽  
Fatty Acid Compositions

In order to establish a chemical fingerprint of vanilla diversity, thirty samples of V. planifolia J. W. Moore and V. tahitensis G. Jackson cured beans from seven producing countries were examined for their aroma and fatty acid contents. Both fatty acid and aroma compositions were found to vary between vanilla species and origins. Vanillin was found in higher amounts in V. planifolia (1.7-3.6% of dry matter) than in V. tahitensis (1.0-2.0%), and anisyl compounds were found in lower amounts in V. planifolia (0.05%) than in V. tahitensis (1.4%-2.1%). Ten common and long chain monounsaturated fatty acids (LCFA) were identified and were found to be characteristic of the vanilla origin. LCFA derived from secondary metabolites have discriminating compositions as they reach 5.9% and 15.8% of total fatty acids, respectively in V. tahitensis and V. planifolia. This study highlights the role of the curing method as vanilla cured beans of two different species cultivated in the same country were found to have quite similar fatty acid compositions.

Quantification of the aromatic potential of ripe fruit of Vanilla planifolia (Orchidaceae) and several of its closely and distantly related species and hybrids

2021 ◽  
Author(s):  
Araceli Pérez-Silva ◽  
Mayra Nicolás-García ◽  
Thomas Petit ◽  
Jean Bernard Dijoux ◽  
María de los Ángeles Vivar-Vera ◽  
...  
Keyword(s):  
Related Species ◽  
Vanilla Planifolia ◽  
Ripe Fruit

scholarly journals Action of vanillin (Vanilla planifolia) on the morphology of tibialis anterior and soleus muscles after nerve injury

2017 ◽  
Vol 15 (2) ◽  
pp. 186-191 ◽  
Author(s):  
Ana Luiza Peretti ◽  
Juliana Sobral Antunes ◽  
Keli Lovison ◽  
Regina Inês Kunz ◽  
Lidyane Regina Gomes Castor ◽  
...  
Keyword(s):  
Nerve Injury ◽  
Tibialis Anterior ◽  
Morphological Analysis ◽  
Morphological Changes ◽  
Control Group ◽  
Vanilla Planifolia ◽  
Morphological Alterations ◽  
Injury Group ◽  
Fiber Atrophy ◽  
Oral Doses

ABSTRACT Objective To evaluate the action of vanillin (Vanilla planifolia) on the morphology of tibialis anterior and soleus muscles after peripheral nerve injury. Methods Wistar rats were divided into four groups, with seven animals each: Control Group, Vanillin Group, Injury Group, and Injury + Vanillin Group. The Injury Group and the Injury + Vanillin Group animals were submitted to nerve injury by compression of the sciatic nerve; the Vanillin Group and Injury + Vanillin Group, were treated daily with oral doses of vanillin (150mg/kg) from the 3rd to the 21st day after induction of nerve injury. At the end of the experiment, the tibialis anterior and soleus muscles were dissected and processed for light microscopy and submitted to morphological analysis. Results The nerve compression promoted morphological changes, typical of denervation, and the treatment with vanillin was responsible for different responses in the studied muscles. For the tibialis anterior, there was an increase in the number of satellite cells, central nuclei and fiber atrophy, as well as fascicular disorganization. In the soleus, only increased vascularization was observed, with no exacerbation of the morphological alterations in the fibers. Conclusion The treatment with vanillin promoted increase in intramuscular vascularization for the muscles studied, with pro-inflammatory potential for tibialis anterior, but not for soleus muscle.

135. Proteomic analysis of in vitrovanilla (Vanilla planifolia) apices subjected to cryoprotective treatments following vitrification approach

Cryobiology ◽  
2010 ◽  
Vol 61 (3) ◽  
pp. 403
Author(s):  
Silvia Valdes-Rodriguez ◽  
Maria Teresa Gonzalez-Arnao ◽  
Armando Guerrero ◽  
Claudia E. Lazaro Vallejo
Keyword(s):  
Proteomic Analysis ◽  
Vanilla Planifolia

Chemotypical variation in Vanilla planifolia Jack. (Orchidaceae) from the Puebla-Veracruz Totonacapan region

2011 ◽  
Vol 59 (5) ◽  
pp. 875-887 ◽  
Author(s):  
Víctor Manuel Salazar-Rojas ◽  
B. Edgar Herrera-Cabrera ◽  
Adriana Delgado-Alvarado ◽  
Marcos Soto-Hernández ◽  
Fernando Castillo-González ◽  
...  
Keyword(s):  
Vanilla Planifolia
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