scholarly journals Biocompatibility of electrospinning polycaprolactone, polylactic acid, their blends and copolymers scaffolds in in vitro tests if mesenchyme stem cells

2021 ◽  
Vol 8 (5) ◽  
pp. 38-49
Author(s):  
A. I. Mishanin ◽  
A. N. Panina ◽  
E. N. Bolbasov ◽  
S. I. Tverdokhlebov ◽  
A. S. Golovkin
Keyword(s):  
Stem Cells ◽  
Polylactic Acid ◽  
Biodegradable Polymers ◽  
In Vitro Study ◽  
Polyglycolic Acid ◽  
In Vitro Tests ◽  
Adhesion Properties ◽  
Acid Copolymer ◽  
Mesenchyme Stem Cells

Background. Biodegradable polymers are one of the most promising groups of materials suitable for creating tissue-engineered scaffolds. The high interest in biopolymers is associated with the possibility of creating scaffolds with desired properties, through the use of mixtures and copolymers. The determination of the key parameters of biocompatibility is the basic purpose for testing created materials.Objective. To perform the comparative in vitro study of biocompatibility properties of biopolymer scaffolds produced using polycaprolactone, polylactic acid, their mixtures and copolymers by electrospinning technology.Design and methods. The adhesion properties and cytotoxicity of scaffolds made from polycaprolactone, polylactic acid, copolymer of L- and D-isoforms of lactic acid, their mixtures and co-polymers with the addition of polyglycolic acid were investigated after scaffolds co-cultivation with human mesenchyme stem cells (MSC).Results. The largest number of spread spindle-shaped MSCs was on the surface of polymers containing polyglycolic acid. Besides, the cells on the surface of the copolymer with polyglycolic acid had the morphology closest to the control. The lowest number of living cells was found on the surface of polylactic acid scaffolds, and the highest on the surface of samples from of polycaprolactone and polylactic acid blend.Conclusion. Thus, all tested polymers had good adhesion properties in experiments with human mesenchyme stem cells were possessed by biodegradable polymers with the addition of polyglycolic acid. 

Comparing the osteogenic potential of schneiderian membrane and dental pulp mesenchymal stem cells: an in vitro study

2021 ◽  
Author(s):  
Antoine Berbéri ◽  
Joseph Sabbagh ◽  
Rita Bou Assaf ◽  
Michella Ghassibe-Sabbagh ◽  
Fatima Al-Nemer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dental Pulp ◽  
In Vitro Study ◽  
Vitro Study ◽  
Osteogenic Potential ◽  
Schneiderian Membrane

Isolation, Characterization, and Differentiation of Stem Cells From Various Dental Sources: An In Vitro Study

2021 ◽  
pp. 232020682110107
Author(s):  
Sandeep S. Katti ◽  
Kishore Bhat ◽  
Chetana Bogar
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Statistical Analysis ◽  
Specific Growth ◽  
Culture Media ◽  
In Vitro Study ◽  
Central Research ◽  
Cell Lineages ◽  
Apical Papilla

Aim: The aim of the current study was to isolate stem cells from various dental sources such as dental pulp, periodontal ligament (PDL), and apical papilla, and to characterize stem cells by staining for the presence/absence of specific surface markers and also to differentiate stem cells into osteogenic, chondrogenic, and adipogenic cell lineages by exposing them to specific growth factors under the ideal conditions. Materials and Methods: A total of 117 samples were included in the study, consisting of 30 pulp, 50 gingival, 35 PDL, and 2 apical papilla samples. The pulp was extirpated and transported to the Central Research Laboratory. Gingival connective tissue was collected from the participants undergoing any crown lengthening procedure or any gingivectomy procedure from the Department of Periodontology. A similar procedure was also followed for apical papilla and PDL. Isolation was done followed by the identification of the cells by immunocytochemistry using different markers. Once the identity of cells was confirmed, these cells were treated with different culture media to attain 70% to 100% confluency. Then the medium was replaced with a conditioning medium containing specific growth factors for differentiation into osteogenic, chondrogenic, and adipogenic cell lineages. Result: In our study, the number of samples collected and processed was 117. The isolation rate of stem cells from the above-collected samples was 70%. Statistical analysis—no statistical analysis was done as there was no variability expected. Conclusion: Our study showed that stem cells could be isolated, differentiated, and characterized from different dental sources.

scholarly journals Similar Features, Different Behaviors: A Comparative In Vitro Study of the Adipogenic Potential of Stem Cells from Human Follicle, Dental Pulp, and Periodontal Ligament

2021 ◽  
Vol 11 (8) ◽  
pp. 738
Author(s):  
Melissa D. Mercado-Rubio ◽  
Erick Pérez-Argueta ◽  
Alejandro Zepeda-Pedreguera ◽  
Fernando J. Aguilar-Ayala ◽  
Ricardo Peñaloza-Cuevas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Periodontal Ligament ◽  
Adipogenic Differentiation ◽  
In Vitro Study ◽  
Mrna Levels ◽  
Dental Tissue ◽  
Periodontal Ligament Stem Cells ◽  
Differentiation Potential

Dental tissue-derived mesenchymal stem cells (DT-MSCs) are a promising resource for tissue regeneration due to their multilineage potential. Despite accumulating data regarding the biology and differentiation potential of DT-MSCs, few studies have investigated their adipogenic capacity. In this study, we have investigated the mesenchymal features of dental pulp stem cells (DPSCs), as well as the in vitro effects of different adipogenic media on these cells, and compared them to those of periodontal ligament stem cells (PLSCs) and dental follicle stem cells (DFSCs). DFSC, PLSCs, and DPSCs exhibit similar morphology and proliferation capacity, but they differ in their self-renewal ability and expression of stemness markers (e.g OCT4 and c-MYC). Interestingly, DFSCs and PLSCs exhibited more lipid accumulation than DPSCs when induced to adipogenic differentiation. In addition, the mRNA levels of adipogenic markers (PPAR, LPL, and ADIPOQ) were significantly higher in DFSCs and PLSCs than in DPSCs, which could be related to the differences in the adipogenic commitment in those cells. These findings reveal that the adipogenic capacity differ among DT-MSCs, features that might be advantageous to increasing our understanding about the developmental origins and regulation of adipogenic commitment.

In vivo and in vitro study of osteogenic potency of endothelin-1 on bone marrow-derived mesenchymal stem cells

2017 ◽  
Vol 357 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Long-Wei Hu ◽  
Xiao Wang ◽  
Xin-Qun Jiang ◽  
Li-Qun Xu ◽  
Hong-Ya Pan
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Endothelin 1

An In Vitro Study of the Effects of Crocin on the Modulation of DSPP, VEGF-A, HLA-G5, STAT3 and CD200 Expression in Human Dental Pulp Stem Cells

2021 ◽  
Author(s):  
Mansoore Saharkhiz ◽  
Fariba Emadian Razavi ◽  
Seyed Mohammad Riahi ◽  
Malaksima Ayadilord ◽  
Zeinab Rostami ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
In Vitro Study ◽  
Dental Pulp Stem Cells ◽  
Vitro Study ◽  
Human Dental Pulp

scholarly journals Erratum: Number of Dental Stem Cells on Root Canal Dentin after Application of Triple Antibiotic Paste or Calcium Hydroxide: An In Vitro Study

2019 ◽  
Vol 13 (02) ◽  
pp. e1-e1
Author(s):  
Konstantinos Gougousis ◽  
Dimitrios G. Giannakoulas ◽  
Vasiliki Taraslia ◽  
Anastasia Agrafioti ◽  
Ema Anastasiadou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
In Vitro Study ◽  
Vitro Study ◽  
Dental Stem Cells ◽  
Triple Antibiotic Paste ◽  
Root Canal Dentin

scholarly journals In Vitro Study of Benzimidazole Derivatives, Tetrahydropyrimidines and Macrocyclic Lactones Therapeutic Efficacy in Dog Hookworms

2018 ◽  
Vol 75 (2) ◽  
pp. 199
Author(s):  
Laura CĂTANĂ ◽  
Raul CĂTANĂ ◽  
Roxana CORA ◽  
Ştefan RĂILEANU ◽  
Mihai CERNEA
Keyword(s):  
Lower Risk ◽  
Regression Line ◽  
In Vitro Study ◽  
Benzimidazole Derivatives ◽  
In Vitro Tests ◽  
Minimal Risk ◽  
Egg Hatch ◽  
Faecal Samples ◽  
Inducing Resistance

The study was conducted using faecal samples from 62 dogs. We tested the ovicidal and larvicidal effects of albendazole (ABZ), mebendazole (MBZ), fenbendazole (FBZ) and flubendazole (FLU) by Egg hatch assay (EHA) and larval development assay (LDA). For pyrantel (PYR) and selamectin (SEL) we tested the larvicidal effects by LDA. In all in vitro tests, benzimidazoles efficacy was low, with a high risk of inducing resistance phenomena. In EHA more than 50% of the hookworm eggs hatched, revealing a low efficacy of all tested benzimidazoles. The regression line was positive for all benzimidazoles, FBZ having the smallest value of the Y parameter (62.62), and lower risk of resistance. When testing the larvicidal effects, a superior efficacy of benzimidazoles was observed. The lowest MIC was for MBZ (0.8672μg/ml). ABZ had a very poor effect (8.46750 μg/ml). The Y parameter showed a lower risk of inducing resistance for MBZ (Y= -64.14) and FBZ (Y= -27.89). Pyrantel and Selamectin were very effective, presenting also a very low risk of inducing resistance phenomena. For PYR and SEL, MIC was 0.2131 μg/ml and 2.7921 μg/ml, respectively. The Y parameter was -448.37 for PYR and -62.74 for SEL, with minimal risk of inducing the adaptive phenomena.

The responses of human adipose-derived mesenchymal stem cells on polycaprolactone-based scaffolds: an in vitro study

2014 ◽  
Vol 11 (3) ◽  
pp. 239-246 ◽  
Author(s):  
Thanaphum Osathanon ◽  
Boontharika Chuenjitkuntaworn ◽  
Nunthawan Nowwarote ◽  
Pitt Supaphol ◽  
Panunn Sastravaha ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Study ◽  
Vitro Study
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