Hepatic transcriptome analysis identifies genes, polymorphisms and pathways involved in the fatty acids metabolism in sheep

Fatty acids (FA) in ruminants, especially unsaturated FA (USFA) have important impact in meat quality, nutritional value, and flavour quality of meat, and on consumer’s health. Identification of the genetic factors controlling the FA composition and metabolism is pivotal to select sheep that produce higher USFA and lower saturated (SFA) for the benefit of sheep industry and consumers. Therefore, this study was aimed to investigate the transcriptome profiling in the liver tissues collected from sheep with divergent USFA content in longissimus muscle using RNA deep-sequencing. From sheep (n = 100) population, liver tissues with higher (n = 3) and lower (n = 3) USFA content were analysed using Illumina HiSeq 2500. The total number of reads produced for each liver sample were ranged from 21.28 to 28.51 million with a median of 23.90 million. Approximately, 198 genes were differentially regulated with significance level of p-adjusted value <0.05. Among them, 100 genes were up-regulated, and 98 were down-regulated (p<0.01, FC>1.5) in the higher USFA group. A large proportion of key genes involved in FA biosynthesis, adipogenesis, fat deposition, and lipid metabolism were identified, such as APOA5, SLC25A30, GFPT1, LEPR, TGFBR2, FABP7, GSTCD, and CYP17A. Pathway analysis revealed that glycosaminoglycan biosynthesis- keratan sulfate, adipokine signaling, galactose metabolism, endocrine and other factors-regulating calcium metabolism, mineral metabolism, and PPAR signaling pathway were playing important regulatory roles in FA metabolism. Importantly, polymorphism and association analyses showed that mutation in APOA5, CFHR5, TGFBR2 and LEPR genes could be potential markers for the FA composition in sheep. These polymorphisms and transcriptome networks controlling the FA variation could be used as genetic markers for FA composition-related traits improvement. However, functional validation is required to confirm the effect of these SNPs in other sheep population in order to incorporate them in the sheep breeding program.

The Discontinuous Elevational Distribution of an Ungulate at the Regional Scale: Implications for Speciation and Conservation

The elevational range where montane species live is a key factor of spatial niche partitioning, because the limits of such ranges are influenced by interspecies interaction, abiotic stress, and dispersal barriers. At the regional scale, unimodal distributions of single species along the elevation gradient have often been reported, while discontinuous patterns, such as bimodal distributions, and potential ecological implications have been rarely discussed. Here, we used extensive camera trap records to reveal the elevation distribution of Himalaya blue sheep (Pseudois nayaur) and its co-existence with other ground animal communities along a slope of Baima Snow Mountain, southwest China. The results show that Himalaya blue sheep exhibited a distinctive bimodal distribution along the elevation gradient contrasting the unimodal distributions found for the other ungulates in Baima snow mountain. A first distributional peak was represented by a population habituating in scree habitat around 4100 m, and a second peak was found in the dry-hot valley around 2600 m. The two distinct populations co-existed with disparate animal communities and these assemblages were similar both in the dry and rainy seasons. The extremely low abundance of blue sheep observed in the densely forested belt at mid-elevation indicates that vegetation rather than temperature is responsible for such segregation. The low-elevation population relied highly on Opuntia ficus-indica, an invasive cactus species that colonized the region six hundred years ago, as food resource. Being the only animal that developed a strategy to feed on this spiky plant, we suggest invasive species may have formed new foraging niche to support blue sheep population in lower elevation hot-dry river valleys, resulting in the geographic separation from the original population and a potential morphological differentiation, as recorded. These findings emphasize the important conservation values of role of ecological functions to identify different taxa, and conservation values of apparent similar species of different ecological functions.

Study of Body Conformation of Carpet Wool Type Chitarangi Sheep of India using Principal Component Analysis

Background: The principal component analysis is applied to identify minimum number of combined variables that account for maximum portion of the variance existing in all variables studied. Chitarangi is a lesser known carpet type wool sheep distributed in Fazilka and Muktsar districts of Punjab, Sri Ganganagar district of Rajasthan and the adjoining areas. The information on body biometry is a prerequisite to characterize the lesser known sheep population available in the country. Hence, it is important to describe the body conformation by recording minimum number of biometric traits. Methods: Body biometry traits of Chitarangi sheep, a lesser known carpet quality wool producing sheep population were studied using Principal Component Analysis. The traits studied were body length (BL), height at wither (HW), chest girth (CG), paunch girth (PG), ear length (EL), face length (FL), face width (FW), tail length (TL) and adult body weight (BW). The data were collected on 297 ewes in the breeding tract of Chitarangi sheep. The descriptive statistics were determined for all the traits. The phenotypic correlations between different body biometric traits were estimated using partial correlations. Principal components were estimated using correlation matrix. Principal component analysis (PCA), a multivariate approach, is used when the recorded traits are highly correlated. Rotation of principal components was through the transformation of the components to approximate a simple structure. Factor analysis using oblique (promax) rotation was used. All the analysis was carried out using the SPSS statistical package. Result: The averages for body weight and biometry traits confirmed large size of Chitarangi animals. Most of the phenotypic correlations amongst the studied traits were positive and significant (p less than 0.01). The three components extracted from nine principal components accounted for 69.06% of the total variance. The first component, which described body size of ewes, accounted for 43.68% of the total variation with high loading for BW, CG, PG, HW, BL and FL. The components two and three explained 13.54 and 11.83% of total variance, respectively. The communalities ranged from 0.490 (FL) to 0.888 (PG). The lower communalities for face length indicated lower contribution of the trait to explain the total variation than others. The study indicates that principal components provided a means of reduction in number of biometric traits to explain body confirmation of adult female Chitarangi sheep.

Scientific Management Practices Followed by Sheep Rearing Farmers in Raichur District of Karnataka, India

Livestock is one of the most remunerative enterprises among the farming community the present study was undertaken to know the scientific sheep management practices by sheep owners in Raichur and Lingasugur taluks of Raichur district. For this purpose, 6 villages from each taluks were selected on the basis of highest sheep population. Ten respondents drawn from each selected village with 120 sample size. The data was collected through structured schedule and the data were analyzed by using appropriate statistical methods. The findings of the study indicated that a large majority of the sheep owners (85.00%) administer the drugs for de worming in lamb management, followed by providing of fresh and clean water (83.33%) and proper bedding material (80.00%) in winter season. With regards feeding management of the sheep, a large majority of the respondents (85.00%) allowed their sheep for grazing in community land Nearly one fifth of the respondents provided green fodder throughout the year as their land comes under the canal irrigation provision, in addition to bore well facilities. Further, 100% of the respondents had proper floor space for the sheep size with cleaning padlock regularly in housing management practices. It is worth to note that 83.00 per cent of them monitor heat detection in sheep and provided treatment to the animals which were suffering from reproductive disorder (87.50%).This is mainly due to the availability of veterinary services in the locality. With regards health management practices, cent percent of the respondents check up the health of the sheep regularly and administer vaccination as scheduled (4/annum) and disinfection of the padlock in order to prevent the disease.

Constraints as Perceived and Suggestions as Expressed by Sheep Rearing Farmers in Raichur District of Karnataka, India

The study was conducted in purposively selected Raichur district of Karnataka. Highest number of sheep population criteria were used in selection of taluks and villages. A total of 120 respondents were selected from 06 selected villages of 2 taluks namely Lingasuguru and Raichur The quantitative and qualitative data were used to collected the information through interview schedule, The present study is highlighted different constraints and suggestion perceived by sheep rearing farmers during management practices simple statistical tools frequency and percentage are used in the study. In socio economic constraints the result found that majority (71.67%) poor knowledge and Ignorance on management practices followed literacy rate (62.05 %), It was found that a high majority (92.50%) unaware insurance services, poor credit facilities (87.50%), shrinkage of grazing land (76.67%) and cost of medicine (61.67%). Suggestion expressed by 69.17 per cent suggested on vaccination followed by keeping quality of buck (65.00%), increase number of veterinary hospital and staff (60.00%) and increase the community grazing land (37.50%), more than three-fourth of sheep farmers suggested discourage the middle man in market, strengthening the existing the animal market (72.50%) and develop new marketing channel (63.33%).

Morphological variability within the indigenous sheep population of Benin

Knowledge of both the genetic diversity and geographical distribution of animal genetic resources is a prerequisite for their sustainable utilization, improvement and conservation. The present study was undertaken to explore the current morphological variability within the sheep population in Benin as a prelude for their molecular characterization. From November 2018 to February 2020, 25 quantitative linear body measurements and 5 qualitative physical traits were recorded on 1240 adult ewes from the 10 phytogeographic zones that comprise the three vegetation zones of Benin. Fourteen morphological indices were calculated based on the linear body measurements. The collected data were first analyzed using multiple comparisons of least-square means (LSmeans), followed by generalized linear model (GLM) procedures, to explore the relationships among the measured morphometric traits and the 10 phytogeographic zones. Next, the presence of any genetic sub-populations was examined using multivariate analytical methods, including canonical discriminant analysis (CDA) and ascending hierarchical clustering (AHC). Univariate analyses indicated that all quantitative linear body measurements varied significantly (P<0.05) across the phytogeographic zones. The highest values (LSmean± standard error) of withers height (68.3±0.47 cm), sternum height (46.0±0.35 cm), and rump height (68.8±0.47 cm) were recorded in the Mekrou-Pendjari zone, the drier phytogeographic zone in the North, whereas the lowest values, 49.2±0.34, 25.9±0.26, and 52.0±0.35 cm, respectively, were recorded in the Pobe zone in the South. Multivariate analyses revealed the prevalence of four distinct sheep sub-populations in Benin. The sub-population from the South could be assimilated to the short-legged and that from the North to the West African long-legged sheep. The two other sub-populations were intermediate and closer to the crossbreeds or another short-legged sub-breed. The proportion of individuals correctly classified in their group of origin was approximately 74%. These results uncovered a spatial morphological variation in the Beninese sheep population along a South-North phytogeographic gradient.

Anaplasma phagocytophilum and Anaplasma ovis–Emerging Pathogens in the German Sheep Population

Knowledge on the occurrence of pathogenic tick-borne bacteria Anaplasma phagocytophilum and Anaplasma ovis is scarce in sheep from Germany. In 2020, owners from five flocks reported ill thrift lambs and ewes with tick infestation. Out of 67 affected sheep, 55 animals were clinically examined and hematological values, blood chemistry and fecal examinations were performed to investigate the underlying disease causes. Serological tests (cELISA, IFAT) and qPCR were applied to all affected sheep to rule out A. phagocytophilum and A. ovis as a differential diagnosis. Ticks were collected from selected pastures and tested by qPCR. Most animals (n = 43) suffered from selenium deficiency and endoparasites were detected in each flock. Anaplasma spp. antibodies were determined in 59% of examined sheep. Seventeen animals tested positive for A. phagocytophilum by qPCR from all flocks and A. phagocytophilum was also detected in eight pools of Ixodes ricinus. Anaplasma phagocytophilum isolates from sheep and ticks were genotyped using three genes (16S rRNA, msp4 and groEL). Anaplasma ovis DNA was identified in six animals from one flock. Clinical, hematological and biochemical changes were not significantly associated with Anaplasma spp. infection. The 16S rRNA analysis revealed known variants of A. phagocytophilum, whereas the msp4 and groEL showed new genotypes. Further investigations are necessary to evaluate the dissemination and health impact of both pathogens in the German sheep population particularly in case of comorbidities.