Assessment of the Genetic Diversity of Black Gram [Vigna Mungo (L.) Hepper] Collections for Yellow Mosaic Virus Resistance Using Simple Sequence Repeat Markers

2019 ◽  
Author(s):  
N Sathees ◽  
D Shoba ◽  
S Saravanan ◽  
S Merina Prem Kumari ◽  
M Arumugam Pillai
Keyword(s):  
Mosaic Virus ◽  
Ssr Markers ◽  
Ssr Marker ◽  
Resistance Breeding ◽  
Yellow Mosaic Virus ◽  
Black Gram ◽  
Simple Sequence Repeat Markers ◽  
Complete Resistance ◽  
Resistant Genotypes ◽  
Simple Sequence

A total of nine black gram genotypes were tested for yellow mosaic virus (YMV) resistance along with other nine biometrical traits. The genotypes VBN 4, KKB-14-015, KKB-14-45 and KKB-14-022 exhibited complete resistance to YMV in field screening. From the molecular characterization, out of 42 SSR markers studied, 15 SSR markers expressed polymorphism. The PIC values ranged from 0.37 (for SSR marker CEDG 024) to 0.79 (CEDG 154) with an average of 0.63. From the cluster analysis, YMV resistant genotypes KKB-14-045 and KKB-14-015 were located on clusters 6 and 7 and the susceptible but agronomic desirable genotypes IC 343943 and IC 436656 were located on cluster 3 which showed the diverse nature of these genotypes. Hence combinations viz., IC 343943 x KKB-14-015, IC 343943 x KKB-14-045, IC 436656 x KKB-14-015 and IC 436656 x KKB-14-045 would be ideal to produce desirable recombinants for YMV resistance breeding in black gram.

scholarly journals Identification of simple sequence repeats (SSR) markers linked to yellow mosaic virus (YMV) resistance in blackgram [Vigna mungo (L). Hepper]

2017 ◽  
Vol 12 (Special-3) ◽  
pp. 812-819
Author(s):  
B. JEEVULA NAIK ◽  
CH. ANURADHA ◽  
P. ANANDA KUMAR ◽  
V. SREEDHAR ◽  
SREENIVASA CHARY
Keyword(s):  
Mosaic Virus ◽  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Vigna Mungo ◽  
Yellow Mosaic Virus ◽  
Simple Sequence

scholarly journals ANALISIS KERAGAMAN GENETIK PLASMA NUTFAH KAKAO (Theobroma cacaoL.) BERDASARKANMARKA SSR / Analysis of Genetic Variability Germplasm of Cacao (Theobroma cacaoL.)Basedon SSR Marker

2020 ◽  
Vol 17 (4) ◽  
pp. 156
Author(s):  
Surti Kurniasih ◽  
Rubiyo Rubiyo ◽  
Asep Setiawan ◽  
Agus Purwantara ◽  
Sudarsono Sudarsono
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Ssr Markers ◽  
Theobroma Cacao ◽  
Simple Sequence Repeat ◽  
Ssr Marker ◽  
Gene Diversity ◽  
Sequence Repeat ◽  
Theobroma Cacao L ◽  
Simple Sequence

<p>Microsatellite or simple sequence repeat (SSR) markers have proven to be an excellent tool for cultivar identification, pedigree analysis, and genetic distance evaluations among organisms. The objectives of this research were to characterize cacao collection of Indonesian Coffee and Cacao Research Institute (ICCRI) and to analyze their genetic diversity using SSR markers. In this research, 39 SSR primer pairs were used to amplify genomic DNA of 29 cacao clones. Amplified SSR fragments for each primer pair were scored as individual band and used to determine genetic distance among evaluated cacao clones. Results of the experiment indicated that all SSR primer pairs evaluated were able to produce SSR markers for 29 cacao clones. The results also indicated that 34 out of 39 microsatellite loci evaluated were polymorphic, while 5 others were monomorphic. The total number of observed alleles among 29 clones was 132. Number of alleles per locus ranged from 4-8, with an average of 5.5 alelles per locus. Results of data analysis indicated that the PIC value was 0.665, the observed heterozigosity (Ho) was 0.651, and the gene diversity (He) was 0.720. The PIC, Ho, and He values were considered high. Genetic distances were evaluated using NTSys version 2.1 and dendrogram was constructed. Results of analysis indicated that 12 cacao clones evaluated were clustered in the first group with diversity coefficient of &lt; 3.75. Nine cacao clones were in the second group but with the same value of diversity coefficient (&lt;7.50). The rest of the cacao clones were in the third group with diversity coefficient of&gt;7.50. Based on those finding, all SSR primer pairs evaluated could be used to analyze cacao genome and be useful for genetic diversity analysis of cacao germplasm. The SSR marker analysis in ICCRI cacao collections resulted in high PIC, high observed heterozygosity, and high genetic diversity.</p><p>Key words: Theobroma cacao L, microsatelite, molecular marker, genetic diversity, heterozygosity</p><p> </p><p><strong>Abstrak</strong></p><p>Marka mikrosatelit atau sekuens sederhana berulang (simple sequence repeat = SSR) terbukti merupakan alat yang bagus untuk identifikasi kultivar, analisis pedigree, dan evaluasi jarak genetik berbagai organisme. Penelitian ini bertujuan untuk:1) karakterisasi kakao koleksi Pusat penelitian Kopi dan Kakao Indonesia menggunakan marka SSR dan 2) analisis keragaman genetik klon-klon kakao koleksi dengan menggunakan marka SSR. Dalam penelitian ini, 39 pasangan primer SSR telah digunakan untuk amplifikasi DNA genomik dari 29 klon kakao. Skoring pita SSR hasil amplifikasi menggunakan masing-masing pasangan primer dilakukan secara terpisah dan digunakan untuk menentukan jarak genetik di antara klon kakao yang dievaluasi. Hasil percobaan menunjukkan bahwa semua pasangan primer SSR yang digunakan mampu menghasilkan pita DNA hasil amplifikasi (marka SSR) untuk 29 klon kakao yang diuji. Hasil penelitian juga menunjukkan bahwa 34 dari 39 lokus SSR yang dianalisis bersifat polimorfik sedangkan lima primer yang lain bersifat monomorfik. Dari 29 klon kakao yang dievaluasi, telah berhasil diamplifikasi sebanyak 132 alel, dengan kisaran antara 4-8 alel/lokus. Rataan jumlah alel per lokus sebanyak 5,50. Hasil analisis data yang dilakukan juga menunjukkan nilai PIC untuk marka SSR yang digunakan sebesar 0,665. Untuk populasi klon kakao yang dievaluasi, diperoleh nilai rataan heterosigositas pengamatan (Ho) sebesar 0,651 dan rataan diversitas gen (He) sebesar 0,720. Nilai PIC Ho dan He yang didapat tergolong tinggi. Berdasarkan analisis keragaman dengan menggunakan program NTSys, diperoleh hasil 12 klon kakao berada dalam grup pertama (koefisien keragaman&lt;3,75) dan9 klon berada dalam grup kedua, dengan koefisien keragaman &lt; 7,50. Sedangkan klon-klon lainnya mempunyai koefisien keragaman &gt; 7,50. Berdasarkan hasil penelitian dan analisis data disimpulkan bahwa marka SSR dapat digunakan untuk menganalisis keragaman genetik plasma nutfah kakao. Tingkat polimorfisme yang dihasilkan marka SSR relatif tinggi. Tingkat heterosigositas plasma nutfah kakao koleksi Puslit Kopi dan Kakao Indonesiarelatif tinggi, dan keragaman genetiknyacukup tinggi.</p><p>Kata kunci : Theobroma cacao L, mikrosatelit, marka molekuler, keragaman genetik, heterosigositas</p>

Validation of CYR-1 marker linked with yellow mosaic virus resistance in black gram (Vigna mungoL. Hepper)

2016 ◽  
Vol 76 (1) ◽  
pp. 104
Author(s):  
K. K. Panigrahi ◽  
T. R. Das ◽  
B. Baisakh ◽  
A. Mohanty ◽  
J. Pradhan
Keyword(s):  
Mosaic Virus ◽  
Virus Resistance ◽  
Yellow Mosaic Virus ◽  
Black Gram

Analysis of sugarcane mosaic virus resistance in maize in an isogenic dihybrid crossing scheme and implications for breeding potyvirus-resistant maize hybrids

Genome ◽  
2006 ◽  
Vol 49 (10) ◽  
pp. 1274-1282 ◽  
Author(s):  
Yongzhong Xing ◽  
Christina Ingvardsen ◽  
Raphael Salomon ◽  
Thomas Lübberstedt
Keyword(s):  
Mosaic Virus ◽  
Gene Action ◽  
Dominant Gene ◽  
Sugarcane Mosaic Virus ◽  
Maize Dwarf Mosaic Virus ◽  
Maize Hybrids ◽  
Complete Resistance ◽  
Additive Gene ◽  
High Level ◽  
Simple Sequence

The gene action of 2 sugarcane mosaic virus (SCMV) resistance loci in maize, Scmv1 and Scmv2, was evaluated for potyvirus resistance in an isogenic background. All 4 homozygous and 5 heterozygous isogenic genotypes were produced for introgressions of the resistant donor (FAP1360A) alleles at both loci into the susceptible parent (F7) genetic background using simple sequence repeat markers. For SCMV and maize dwarf mosaic virus (MDMV), virus symptoms appeared rapidly in the 3 homozygous genotypes, with susceptibility alleles fixed at 1 or both loci. Although the 9 isogenic genotypes revealed a high level of resistance to Zea mosaic virus (ZeMV), the same 3 homozygous genotypes were only partially resistant. This indicates that 1 resistance gene alone is not sufficient for complete resistance against SCMV, MDMV, and ZeMV. Scmv1 showed strong early and complete dominant gene action to SCMV, but it gradually became partially dominant. Scmv2 was not detected at the beginning, showing dominant gene action initially and additive gene action at later stages. Both genes interacted epistatically (for a high level of resistance, at least 1 resistance allele at each of both loci is required). This implies that double heterozygotes at the 2 loci are promising for producing SCMVresistant hybrids. Results are discussed with respect to prospects for isolation of SCMV and MDMV resistance genes.

scholarly journals Development of SSR Marker Set to Identify Fourty Two Indonesian Soybean Varieties

2016 ◽  
Vol 11 (2) ◽  
pp. 49
Author(s):  
Andari Risliawati ◽  
Eny I. Riyanti ◽  
Puji Lestari ◽  
Dwinita W. Utami ◽  
Tiur S. Silitonga
Keyword(s):  
Ssr Markers ◽  
Ssr Marker ◽  
Variety Identification ◽  
Polymorphism Analysis ◽  
Dna Fingerprints ◽  
Soybean Variety ◽  
Genetic Purity ◽  
Electrophoresis System ◽  
Simple Sequence ◽  
Genetic Analyzer

<p>Profile of molecular marker can be used for variety identification, genetic purity monitoring of germplasm and additional<br />requirement in proposing intellectual property protection. DNA fingerprinting of soybean had been applied at the ICABIOGRADIAARD<br />since 2004 using simple sequence repeat (SSR) markers which were run automatically by CEQ 8000 Genetic Analyzer<br />platform based on capillary electrophoresis system. This method had produced unique DNA fingerprints of the varieties tested,<br />but the marker set to efficiently identify the varieties had not yet been developed. This study aimed to develop a set of SSR<br />markers as a tool to identify the Indonesian soybean varieties. Fourty two soybean varieties were analyzed using 14 random SSR<br />markersA total of 168 alleles that were obtained from the polymorphism analysis. The average of polymorphic information<br />content (PIC) value observed was 0.7337 per SSR locus. Based on marker reproducibility rate, PIC value, number of rare alleles,<br />frequency of dominant alleles, and percentage of SSR fragment detected by genetic analyzer, we identified five SSR markers i.e.<br />Satt414, Satt147, Satt308, Satt009, and Satt516 as a SSR marker set to be used for soybean variety identification purposes. This<br />marker set was used to develop the identity (ID) of the 42 Indonesian soybean varieties.</p>

scholarly journals Development of Yellow Mosaic Virus Resistant Genotypes in Urdbean TNAU blackgram VBN 6: A high yielding blackgram variety with resistant to Mungbean Yellow Mosaic Virus

2019 ◽  
pp. 180-186
Author(s):  
Pandiyan M ◽  
Senthil N ◽  
Manivannan N ◽  
Karthikeyan A ◽  
Sivakumar C ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Tamil Nadu ◽  
Yellow Mosaic Virus ◽  
Yield Potential ◽  
Average Yield ◽  
High Protein Content ◽  
Pod Borer ◽  
Mungbean Yellow Mosaic Virus ◽  
Leaf Curl Virus ◽  
Resistant Genotypes

The Blackgram culture VBG04-014 is a cross derivative of Vamban 1 x Vigna mungo var. silvestris 1 released as variety TNAU Blackgram VBN(Bg)6, it is maturing in 65-70 days and suited for cultivation under both rainfed and irrigated conditions. It has an average yield potential of 871 Kg per hectare. This culture is resistant to Yellow Mosaic Virus, Leaf Curl Virus and less damage of pod borer. It possesses desirable characters like high protein content (21.1%). Grains are medium sized with black in colour. It is recommended for cultivation in Tamil Nadu. Keywords: VBG04-014; Blackgram; Yellow Mosaic Virus; Rainfed; Irrigated

scholarly journals Circumscription of the Sibbaldia procumbens complex (Potentilleae: Rosaceae) in China based on evidence from simple sequence repeat markers and morphology

2019 ◽  
Vol 191 (3) ◽  
pp. 305-314 ◽  
Author(s):  
Huajie Zhang ◽  
Tao Feng ◽  
Jacob B Landis ◽  
Xu Zhang ◽  
Aiping Meng ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Species Complex ◽  
Population Level ◽  
Molecular Data ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Stable Variation ◽  
Simple Sequence

Abstract In this study we aimed to delimit the boundaries of the Sibbaldia procumbens species complex, integrating morphological, geographical and molecular data at the population level. In total, 246 individuals were tested with simple sequence repeat (SSR) markers, and individuals were classified into three clusters. Stable variation in the morphology of leaflets, episepal and achenes was found between individuals from eastern and western Tibet, and this corresponds to the observed genetic structure when K = 2. The combined analysis of morphology and SSRs suggests three species should be recognized in the complex in China (S. procumbens, S. cuneata and S. aphanopetala). The ranges of these three species are mostly geographically separate from each other. Sibbaldia aphanopetala has often been treated as a variety of S. procumbens, but here we restore it to species rank.

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