Inheritance Pattern of Mungbean Yellow Mosaic Virus (MYMV) Disease Resistance in Blackgram [Vigna mungo (L.) Hepper]

2021 ◽  
Author(s):  
K. Vadivel ◽  
N. Manivannan ◽  
A. Mahalingam ◽  
V.K. Satya ◽  
S. Ragul
Keyword(s):  
Disease Resistance ◽  
Mosaic Virus ◽  
Tamil Nadu ◽  
Gene Action ◽  
Disease Incidence ◽  
Yellow Mosaic Virus ◽  
Inheritance Pattern ◽  
Complementary Gene ◽  
Mungbean Yellow Mosaic Virus ◽  
Complementary Gene Action

Background: Mungbean yellow mosaic virus (MYMV) disease is the most destructive disease in blackgram. Development of MYMV resistant varieties is one of the best possible solutions to avoid the yield reduction in blackgram. There are conflicting reports on the genetics of resistance to MYMV disease claiming that it is controlled by both dominant and recessive genes. Hence the present study was aimed to understand the inheritance pattern of the MYMV disease resistance in eight crosses of blackgram.Methods: Parents, F1 and F2 generation of eight cross combinations were raised during July - Sep, 2018 at National Pulses Research Centre, Tamil Nadu Agricultural University, Vamban, Tamil Nadu. An infector row of CO 5 was raised to intensify the MYMV disease pressure after every eight rows. Based on disease incidence on 60th day after sowing, two phenotypic classes were formed among F2 plants with the scales of (1 to 3) as resistant phenotype and (4 to 9) as susceptible phenotype. The goodness of fit to Mendelian segregation ratio for MYMV disease resistance in the segregating population was tested by Chi square test (Stansfield, 1991).Result: The MYMV disease incidence was tri-genically controlled with inhibitory gene action in four crosses viz., MDU 1 x Mash 114, CO5 x Mash 114, MDU 1 x VBN 6 and CO 5 x VBN 6. Complementary gene action with two genes was observed in four crosses viz., MDU 1 x Mash 1008, CO 5 x Mash 1008, MDU 1 x VBN 8 and CO 5 x VBN 8. Differences in number of genes were observed due to the presence of recessive inhibitory gene in both male and female parents of the crosses which had complementary gene action for MYMV disease. The putative gene symbols assigned for the six genotypes viz., S1S1S2S2ii (MDU 1 and CO 5), s1s1s2s2II (Mash 114 and VBN 6) and s1s1s2s2ii (Mash 1008 and VBN 8), respectively. 

scholarly journals Inheritance and Gene Effect of Resistance to Mungbean Yellow Mosaic Virus (MYMV) in Mungbean [Vigna radiata (L.) Wilczek]

2020 ◽  
Author(s):  
K.S. Win ◽  
S. Win ◽  
T.M. Htun ◽  
N.K.K. Win ◽  
K.S. Oo
Keyword(s):  
Disease Resistance ◽  
Mosaic Virus ◽  
Recessive Gene ◽  
Yellow Mosaic Virus ◽  
Inheritance Pattern ◽  
Gene Effects ◽  
Mungbean Yellow Mosaic Virus ◽  
Production Knowledge ◽  
Resistant Genotypes ◽  
Mode Of Inheritance

Background: Mungbean Yellow Mosaic Virus is one of the major constraints in mungbean production. Knowledge of mode of inheritance and gene effects of MYMV resistance is very useful and effective for the development of genotypes resistant to disease or incorporation of resistance into the desirable promising genotypes whichlack of disease resistance. Methods: In order to estimate inheritance pattern of MYMV disease resistance in mungbean, the study was conducted in summer season (2019) under natural condition. Six generations such as Pl, P2, Fl, BC1, BC2 and F2 of six combinations [two resistant genotypes (7639 and 10266) and three susceptible genotypes (7621, 10257 and R-021018)] were studied inheritance pattern of resistance to MYMV in segregation population. Result: Based on the result from mode of inheritance pattern of MYMV resistance, it can be concluded that single recessive gene is controlled the resistance of MYMV and susceptible behavior indicated as dominant over resistant. Additive gene action was the major role for the selection of MYMV resistance. Some differences in the expression of gene contributing for MYMV resistance from others findings might be attributed due to the sources of resistant genotypes which have different nature of resistant gene used in this investigation. 

Identification and Validation of Quantitative Trait Loci of Mungbean Yellow Mosaic Virus Disease Resistance in Blackgram [Vigna mungo (L). Hepper]

2021 ◽  
Author(s):  
K. Vadivel ◽  
N. Manivannan ◽  
A. Mahalingam ◽  
V.K. Satya ◽  
C. Vanniarajan ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Quantitative Trait Loci ◽  
Mosaic Virus ◽  
Phenotypic Variation ◽  
Quantitative Trait ◽  
Tamil Nadu ◽  
Virus Disease ◽  
Yellow Mosaic Virus ◽  
Disease Score ◽  
Mungbean Yellow Mosaic Virus

Background: Blackgram [Vigna mungo (L.) Hepper] is an important food legume crop of India. Mungbean yellow mosaic virus (MYMV) disease is the major problem in blackgram. The disease can reduce seed yield upto 100% or even kill a plant infected at an early vegetative stage. The most effective way to prevent the occurrence of this disease is to develop genetically resistant cultivars of blackgram. However, a major difficulty in breeding MYMV disease resistant in blackgram is field screening for the virus disease. Hence identification of QTL followed by Marker-assisted selection (MAS) is highly useful for genetic improvement of crops. With this background, a study was made for identification as well as validation of quantitative trait loci (QTL) for MYMV disease resistance in blackgram.Methods: A total of 112 F2:3 lines were evaluated for MYMV disease resistance along with parents viz., MDU 1 (MYMV disease susceptible) and Mash 1008 (MYMV disease resistant) at the National Pulses Research Centre, Tamil Nadu Agricultural University, Vamban, Tamil Nadu during July-September 2018 under Augmented Design in the field. Each line was sown in one row of 3 m in length with a spacing of 30 cm as between row and 10 cm as within row. Susceptible genotypes CO 5 and MDU 1 were sown as disease spreader rows after every eight rows and also around the plots. The MYMV disease score was recorded on 60 DAS, by using phenotype rating scale from 1 (resistant) to 9 (highly susceptible), as suggested by Singh et al. (1995). The mean of each progeny was calculated and used as phenotypic data. A total of 525 SSR primers were used to test polymorphism between parents MDU 1 and Mash 1008. Genotyping was carried out for 112 F2:3 RILs of the cross MDU 1 x Mash 1008 with 35 polymorphic SSR markers. Linkage and QTL analyses were performed using QTL IciMapping (version 4.1.0.0) (Wang et al. 2016) and QGene 4.4.0 (Joehanes and Nelson 2008) respectively. Two mapping populations MDU 1 x Mash 114 and CO 5 x VBN 6 in F2:3 and F2 generations respectively were used in this study to validate the identified QTL regions.Result: QTL study indicated the presence of two major QTLs for MYMV disease score in LG 2 and LG 10 at 60 DAS with 20.90 and 24.90% of phenotypic variation respectively. Validation of these QTLs in two other mapping population indicated that QTL on LG 10 was validated with high phenotypic variation of 45.40-46.00%. Hence it may conclude that qmymv10_60 may be utilized for MAS/MABC with assured improvement on MYMV disease resistance in blackgram.

scholarly journals Development of high yielding with durable resistance against Mungbean yellow mosaic virus genotypes in Blackgram

2019 ◽  
pp. 187-193
Author(s):  
Pandiyan M ◽  
Senthil N ◽  
Krishnaveni A ◽  
Sivakumar C ◽  
Singh BB ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Mosaic Virus ◽  
Tamil Nadu ◽  
Durable Resistance ◽  
Yellow Mosaic Virus ◽  
Yield Potential ◽  
Average Height ◽  
Crude Fibre ◽  
Mungbean Yellow Mosaic Virus ◽  
Virus Genotypes

The Blackgram culture VBG04-008 is a cross derivative of blackgram Vamban 3 x Vigna mungo var. silvestris 8 is released as TNAU blackgram VBN (Bg) 7 maturing in 65-70 days with an average height of 17 cm and suited for cultivation under both under rainfed and irrigated conditions. It has a yield potential of 981 Kg per hectare. This culture is resistant to Yellow Mosaic Virus, Powdery mildew and Leaf Curl Virus and less damage of pod borer. It possesses desirable characters like high protein content (21.05%), crude fibre (5.90g/100g) and iron (3.76 mg/100g). Grains are medium sized with black in colour. It is recommended for cultivation in Tamil Nadu, Andhra Pradesh, Karnataka and Orissa. Keywords: VBG04-008; Blackgram; VBN 7 Mung Bean Yellow Mosaic Virus; Powdery mildew-Rainfed; Irrigated

scholarly journals Management of Yellow Mosaic Disease of Mungbean Against Mungbean Yellow Mosaic Virus (MYMV) in Bangladesh

2018 ◽  
Vol 1 (2) ◽  
pp. p100
Author(s):  
Md. S. Islam ◽  
Md. B. Hossain ◽  
Saleh A. Shahriar ◽  
Fatema Begum ◽  
Md. N. H. Sani
Keyword(s):  
Mosaic Virus ◽  
Disease Severity ◽  
Agricultural Research ◽  
Disease Incidence ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Economic Damage ◽  
Yellow Mosaic Disease ◽  
Yield Attributes ◽  
Mungbean Yellow Mosaic Virus

The prime aim of the study was to manage of Yellow mosaic disease of mungbean against Mungbean yellow mosaic virus (MYMV) by using one newly release botanical nutrient and through three selected insecticides. BARI (Bangladesh Agricultural Research Institute) released variety BARI mung-5, three insecticides (Imidacloprid, Acmix and Sobicron) and one botanical nutrient PPN (Peak performance nutrients) were used in the experiment. The plants were grown for pulse production and natural inoculums were relied upon for the infection of MYMV. Growth parameters, yield attributes and physiological features were significantly influenced by the application of selected insecticides and PPN combinations. Disease incidence and disease severity of MYMV were significantly varied among the treatments. Application of Imidacloprid with PPN combination gave the lowest disease incidence (3.13, 5.24 and 6.24% per plot and 14.33, 15.49 and 21.87% per plant) at 30, 40 and 50 DAS, respectively while the highest disease incidence (7.77, 13.70 and 19.24% per plot and 39.33, 48.20 and 56.63% per plant) were found in control at 30, 40 and 50 DAS, respectively. Application of Imidacloprid with PPN also gave the lowest disease severity (5.00, 6.00 and 13.33% at 30, 40 and 50 DAS, respectively while the highest disease severity (27.33, 35.00 and 45.00%) at 30, 40 and 50 DAS, respectively were measured in control treatment when no insecticides and PPN was used. If the disease is established once in the field then it is difficult to manage. As the disease is transmitted by vector (whitefly), the growers are suggested to control the vector populations before reaching economic damage and severe disease infection.

scholarly journals Development of Yellow Mosaic Virus Resistant Genotypes in Urdbean TNAU blackgram VBN 6: A high yielding blackgram variety with resistant to Mungbean Yellow Mosaic Virus

2019 ◽  
pp. 180-186
Author(s):  
Pandiyan M ◽  
Senthil N ◽  
Manivannan N ◽  
Karthikeyan A ◽  
Sivakumar C ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Tamil Nadu ◽  
Yellow Mosaic Virus ◽  
Yield Potential ◽  
Average Yield ◽  
High Protein Content ◽  
Pod Borer ◽  
Mungbean Yellow Mosaic Virus ◽  
Leaf Curl Virus ◽  
Resistant Genotypes

The Blackgram culture VBG04-014 is a cross derivative of Vamban 1 x Vigna mungo var. silvestris 1 released as variety TNAU Blackgram VBN(Bg)6, it is maturing in 65-70 days and suited for cultivation under both rainfed and irrigated conditions. It has an average yield potential of 871 Kg per hectare. This culture is resistant to Yellow Mosaic Virus, Leaf Curl Virus and less damage of pod borer. It possesses desirable characters like high protein content (21.1%). Grains are medium sized with black in colour. It is recommended for cultivation in Tamil Nadu. Keywords: VBG04-014; Blackgram; Yellow Mosaic Virus; Rainfed; Irrigated

scholarly journals Seed Borne Nature of Mungbean yellow mosaic virus (MYMV) in Blackgram in Tamil Nadu

2021 ◽  
pp. 58-67
Author(s):  
K. Kamesh Krishnamoorthy ◽  
V. G. Malathi ◽  
P. Renukadevi ◽  
S. Mohan Kumar ◽  
M. Raveendran ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Tamil Nadu ◽  
Infected Plant ◽  
Susceptible Variety ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Resistant Varieties ◽  
Mungbean Yellow Mosaic Virus ◽  
Cropping Seasons ◽  
Agroclimatic Zones

The yellow mosaic disease of blackgram caused by Mungbean yellow mosaic virus has emerged as a serious threat to pulses production especially in the South Eastern Asia.  Seed borne nature of MYMV in blackgram seeds was determined using the seeds harvested from a MYMV resistant (either VBN-6 or VBN-8) and susceptible blackgram (CO-5) varieties grown in three different agroclimatic zones of Tamil Nadu in India for three consecutive cropping seasons namely, Rabi 2018 (October- December), Summer 2019 (March-May) and Kharif 2019 (June- August) at three different time intervals viz., 20, 40 and 60 days after sowing (DAS). Seed borne nature of MYMV was observed only in the susceptible variety CO-5 and was absent in the   resistant varieties. Transmission of MYMV from infected plant to seeds was observed in all the three parts of the seeds viz., seed coat, cotyledon and embryo. Seeds from infected plants also showed abnormalities like shrinking, discolouration, ill filling inside pods and misshapen appearance.

scholarly journals Screening of Urdbean Germplasm for High Seed Yield Coupled with Resistance to Mungbean yellow mosaic virus (MYMV)

2020 ◽  
pp. 100-106 ◽  
Author(s):  
Sunita Kumari ◽  
Sangita Sahni ◽  
Bishun Deo Prasad
Keyword(s):  
Mosaic Virus ◽  
Seed Yield ◽  
Disease Incidence ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Sources Of Resistance ◽  
Production Constraints ◽  
Mungbean Yellow Mosaic Virus ◽  
Disease Free ◽  
High Seed Yield

Yellow mosaic disease (YMD) caused by Mungbean yellow mosaic virus (MYMV) is one of the most destructive biotic production constraints in urdbean. Development and introduction of resistant cultivars with high seed yield are considered as the most economical and eco-friendly option to manage YMD, for which availability of stable sources of resistance with high seed yield is a pre-requisite. A set of one hundred twenty eight genotypes of urdbean including a susceptible check were evaluated against MYMV in the field for two consecutive years during summer and kharif 2015-2016 under natural condition of disease incidence. There was considerable variation among the genotypes with respect to disease reaction. Out of 128 genotypes tested, only five genotype namely KU 96-3, NDU 12-1,  NIRB 002,  NIRB 003 & NIRB 004 were found to be disease free, nineteen genotypes (IPU 10-23,  IPU 11-01, KPU 34, KUG 540,  KUG 586, Mash-338, NDU 12-2, NDU 12-300, NDUZ 14-21,OBG 35,  PU 09-35, Shekhar 3,  UH 07-06, Uttara, VBG 10-008, VBG 11-053,VBN (BG) 3, VBN 6 & Vijay) found to be highly resistant and twenty two genotypes (IGKU 02-1, Kopergaon,  KPU 12, KPU 13, KPU 14, KPU 16, KPU 33, KPU 7, KPU 8, KU 363,  NDU 11-01,Palampur 93, Pant U 19,  PU 08-05, PU 20, PU 22, RUG-44, Sekhar 2 , TU 67, UG 218, VBG 09-005 & WBU 108) showed highly resistance or resistance consistently in both the seasons. However, eight genotypes i.e. Uttara, PU-31, KU-363, KUG 540, UH 07-06, KUG 503, WBU 108 & Shekhar 33 were found to be superior for seed yield as well as resistant to MYMV. Among these lines, UH 07-06 and KUG 503 give highest seed yield in Kharif and summer.

Identification and Confirmation of Resistance in Mungbean [Vigna radiata (L.) Wilczek] Derivatives to Mungbean Yellow Mosaic Virus (MYMV)

2020 ◽  
Author(s):  
B. Madhumitha ◽  
K. Eraivan Arutkani Aiyanathan ◽  
M. Raveendran ◽  
M. Sudha
Keyword(s):  
Mosaic Virus ◽  
Mung Bean ◽  
Tamil Nadu ◽  
Environmental Changes ◽  
Infectious Clone ◽  
Yellow Mosaic Virus ◽  
Whitefly Transmission ◽  
Access Period ◽  
Mungbean Yellow Mosaic Virus ◽  
Interspecific Derivatives

Background: Mung bean Yellow Mosaic Virus (MYMV) is found to be one of the prime viral diseases of mungbean in Tamil Nadu state. Screening for MYMV resistance in field condition always remains a hassle for breeding society. The peculiar MYMV symptoms often failed in the field due to some factors such as environmental changes, whitefly genotypes, host factors etc. With the above perspective, the present study aimed to screen the mung bean derivatives against MYMV through a novel in vitro agroinoculation technique and further substantiation through whitefly transmission.Methods: Four interspecific derivatives (VGGRU 1, VGGRU 2, VGGRU 3 and VGGRU 4) generated by making crosses between mungbean VRM (Gg) 1 and rice bean (TNAU RED) along with the susceptible check VRM (Gg) 1 were agroinoculated with the MYMV infectious clone VA 239 (KA30 DNA A + KA27 DNA) and are further substantiated through whitefly transmission studies from the artificially reared whiteflies. Result: The agroinoculation results revealed that among the four interspecific derivatives, VGGRU 1 was found to be completely resistant to MYMV. The substantiation of the obtained result through whitefly transmission also revealed that 24 h Acquisition Access Period (AAP) and 24 h Inoculation Access Period (IAP) with Bemisia tabaci able to cause 65% infectivity in susceptible plant VRM (Gg) 1 and zero infectivity in VGGRU 1 and the results were PCR confirmed for the presence of viral DNA.

scholarly journals Screening of Mungbean (Vignaradiata L. Wilczek) Genotypes for Resistance to Mungbean Yellow Mosaic Virus under Field Condition

2021 ◽  
pp. 39-45
Author(s):  
Yugandhar Gokidi ◽  
M. N. Singh ◽  
Ashok Singamsetti ◽  
Swathi Lekkala
Keyword(s):  
Mosaic Virus ◽  
Field Condition ◽  
Seed Quality ◽  
Disease Incidence ◽  
Pigeon Pea ◽  
Yellow Mosaic Virus ◽  
Grain Legume ◽  
Legume Crop ◽  
Mungbean Yellow Mosaic Virus ◽  
The Difference

Greengram [Vignaradiata(L.) Wilczek] is an economically important grain legume crop next to chickpea, pigeon pea and urad gram. Among the biotic factors, Mungbean Yellow Mosaic Virus (MYMV) is reported to be the most destructive viral diseases, which may reduce the seed quality as well as the yield losses up to 100 per cent. It is transmitted through whitefly (Bemisiatabaci) in a persistent manner throughout Asia, including India. A set of forty-two diverse mungbean genotypes were sown in two replications using a Randomized Block Desigh (RBD) during the summer season of 2015. The infector row method was adopted to evaluate a set of mungbean genotypes to know the difference in the level of resistance against MYMV infection under field condition during summer, 2015. Percent Disease Incidence (PDI) was calculated at 30 DAS and 50 DAS respectively. It varied from 2.18 to 64.77% and 5.38 to 76.87% at 30 DAS and 50 DAS respectively in summer, 2015. On the basis of disease severity recorded, the mungbean genotypes were classified in to five disease infection categories. Out of the forty-two mungbean genotypes, thirteen genotypes viz., Pusa 0672, IPM 205-7, HUM 8, KM 2245, IPM-2-03, ML 1464, KM 2241, PDM-139, TARM-1, HUM 26, Meha, HUM 16 and IPM 409-4 were found to be resistant and may provide the source of resistance against MYMV to develop mapping population for molecular breeding, development of molecular markers, QTL identification for MYMV resistance, as well as development of MYMV resistant varieties.

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