Biosolubilization of rock phosphates in a bioreactor using a microbial consortium from rhizospheric soils: An analysis

2018 ◽  
Vol 35 (4) ◽  
pp. 184-191
Author(s):  
C. Xiao ◽  
Q. Wang ◽  
B. Feng ◽  
G. Xu ◽  
R. Chi
Author(s):  
P.W. Shannon

Increasing material, processing, and distribution costs have raised superphosphate prices to a point where many farms cannot support the costs of meeting maintenance phosphate requires men& Alternatives to superphosphate, particularly those that have lower processing costs and contain more P, may offer a solution to the problem provided they are agronomically as effective. Phosphate rock may indeed be such an alternative. Preliminary results from a series of five trials in Northland show that on soils of moderate P fertility, with low phosphate retention (PR) and high pH (5.9.6.0), initial pasture growth responses to rock phosphates are smaller than those from single or triple superphosphate. On one soil of higher PR and lower pH, the differences in yield between the rock-phosphates and the super. phosphates were smaller. Of the rock phosphates tested, Sechura and North Carolina (unground and ungranulated) tended to be more effective than ground and granulated Chatham Rise phosphorite. The effect on production of applying fertilisers once every three years, as opposed to annual applications is being investigated using triple superphosphate and Sechura phosphate rock. After two years, production levels appear largely unaffected by differences in application frequency. A comparison of locally-produced superphosphate with a reference standard showed that both performed similarly, indicating that the local product was of satisfactory quality.


Agrotek ◽  
2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Antonius Suparno ◽  
Dwiana Wasgito Purnomo ◽  
Karyoto Sardi Amat

The research was conducted at Soroan, Ayamaru District, South Sorong, Papua. �The objective of the study was to observe the diversity of Arbuscular Mycorrhiza Fungi (AMF) that symbiosis with cultivated plants at the Ayamaru rock phosphates deposit. Based on the observation, there were four AMF associated with nine cultivated plants at the Ayamaru rock phosphates deposit, namely genus Glomus, Acaulospora, Sclerocystis and Gigaspora. Genus Glomus had the greatest diversity (13 types) followed by Acaulospora which comprised of seven types.� On the other hand, the diversity of genus Sclerocystis and Gigaspora only consisted of two types and one type, respectively.


Author(s):  
Li-Li Jiang ◽  
Feng-Yi Liu ◽  
Wei Yang ◽  
Chang-Li Li ◽  
Bao-Wei Zhu ◽  
...  

Author(s):  
Pradeep Kumar Dash ◽  
Soumya Ranjan Padhy ◽  
Pratap Bhattacharyya ◽  
Ankita Pattanayak ◽  
Soumya Routray ◽  
...  

2021 ◽  
pp. 2100713
Author(s):  
Lin Liu ◽  
Maedeh Mohammadifar ◽  
Anwar Elhadad ◽  
Mehdi Tahernia ◽  
Yunxiang Zhang ◽  
...  

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Chaofeng Li ◽  
Xiaofeng Lin ◽  
Xing Ling ◽  
Shuo Li ◽  
Hao Fang

Abstract Background The biomanufacturing of d-glucaric acid has attracted increasing interest because it is one of the top value-added chemicals produced from biomass. Saccharomyces cerevisiae is regarded as an excellent host for d-glucaric acid production. Results The opi1 gene was knocked out because of its negative regulation on myo-inositol synthesis, which is the limiting step of d-glucaric acid production by S. cerevisiae. We then constructed the biosynthesis pathway of d-glucaric acid in S. cerevisiae INVSc1 opi1Δ and obtained two engineered strains, LGA-1 and LGA-C, producing record-breaking titers of d-glucaric acid: 9.53 ± 0.46 g/L and 11.21 ± 0.63 g/L d-glucaric acid from 30 g/L glucose and 10.8 g/L myo-inositol in fed-batch fermentation mode, respectively. However, LGA-1 was preferable because of its genetic stability and its superior performance in practical applications. There have been no reports on d-glucaric acid production from lignocellulose. Therefore, the biorefinery processes, including separated hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF) and consolidated bioprocessing (CBP) were investigated and compared. CBP using an artificial microbial consortium composed of Trichoderma reesei (T. reesei) Rut-C30 and S. cerevisiae LGA-1 was found to have relatively high d-glucaric acid titers and yields after 7 d of fermentation, 0.54 ± 0.12 g/L d-glucaric acid from 15 g/L Avicel and 0.45 ± 0.06 g/L d-glucaric acid from 15 g/L steam-exploded corn stover (SECS), respectively. In an attempt to design the microbial consortium for more efficient CBP, the team consisting of T. reesei Rut-C30 and S. cerevisiae LGA-1 was found to be the best, with excellent work distribution and collaboration. Conclusions Two engineered S. cerevisiae strains, LGA-1 and LGA-C, with high titers of d-glucaric acid were obtained. This indicated that S. cerevisiae INVSc1 is an excellent host for d-glucaric acid production. Lignocellulose is a preferable substrate over myo-inositol. SHF, SSF, and CBP were studied, and CBP using an artificial microbial consortium of T. reesei Rut-C30 and S. cerevisiae LGA-1 was found to be promising because of its relatively high titer and yield. T. reesei Rut-C30 and S. cerevisiae LGA-1were proven to be the best teammates for CBP. Further work should be done to improve the efficiency of this microbial consortium for d-glucaric acid production from lignocellulose.


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