scholarly journals Genetic Study of Phenylthiocarbamide (PTC) Taste Sensitivity In Population of The Osing in Kemiren Village-Banyuwangi

2021 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Fenny Indriani ◽  
Rike Oktarianti ◽  
Syubbanul Wathon
Keyword(s):  
Genetic Study ◽  
Gene Frequency ◽  
Serial Dilution ◽  
Allele Frequencies ◽  
Genotype Frequency ◽  
Taste Sensitivity ◽  
Dilution Method ◽  
Serial Dilution Method ◽  
Different Populations ◽  
Age Range

The ability to taste phenylthiocarbamide (PTC), is autosomal trait inherited in a simple Mendelian recessive pattern. The frequency of Taster and non-Taster allele is varies in different populations. The purpose of the research is to investigate the prevalence, gene frequency and genotype frequency of taster (T) and non taster (ts of Osing population in Kemiren-Banyuwangi. PTC serial dilution method was used to assess the PTC Taster and non-Taster phenotypes. The Hardy–Weinberg method was used to determine allele frequencies. The total of samples were 227 people, male were 117 and female were 110 with age range of 15–30 years were randomly selected. The result showed that the Osing population as Taster were 210 (92,52%) and non Taster were 17samples (7,48%) . The allele frecuency of Taster (T) was 0,73 and non Taster (t) was 0,27 respectively. The genotype frequency of dominant Taster (TT) was 0,54, heterozygosity Taster (Tt) was 0,39, and genotype of non Taster (tt) was 0,07.

scholarly journals ANTIBIOTICS RESISTANCE OF CORYNEBACTERIUM NON DIPHTHERIAE STRAINS

2017 ◽  
pp. 3-8
Author(s):  
G. G. Kharseeva ◽  
N. A. Voronina ◽  
T. D. Gasretova ◽  
O. I. Sylka ◽  
S. Yu. Tyukavkina
Keyword(s):  
Serial Dilution ◽  
Urogenital Tract ◽  
Antibiotics Resistance ◽  
Dilution Method ◽  
Frequency Of Occurrence ◽  
Serial Dilution Method ◽  
Resistant Strains

Aim. Study the frequency of occurrence of antibiotics resistant strains of various species of Corynebacterium non diptheriae. Materials and methods. C.pseudodiphtheriticum, C.pseudotuberculosis, C.xerosis, C.amycolatum, C.striatum, C.ulcerans strains isolated from patients with pathologies of respiratory and urogenital tract, as well as individuals taking prophylaxis examination were used. Sensitivity to antibacterial preparations was determined by the serial dilution method. Results. The highest number of Corynebacterium non diptheriae strains displayed resistance to benzylpenicillin (54.8%) and lincomycin (50.7%), and lowest - to cefotaxime, cefazolin (6.8%) and vancomycin (13.7%). The highest number of antibiotics resistant strains were detected among members of C.pseudotuberculosis {100%), C.xerosis (96.0%) and C. pseudodiphtheriticum (81.0%) species. Polyresistant strains were detected most frequently among C.xerosis, C.amycolatum and C.striatum species. Strains of Corynebacterium non diptheriae most frequently displayed resistance to 1 or 2 antibacterial preparations (24.7%), less frequently - to 3 (20.5%), 4 (13.7%), 5 (4.1%) and 6 (1.4%) preparations. Conclusion. The amount of antibiotics resistant strains of Corynebacterium non diptheriae is large (89.0%) and non-similar in various species.

Research on Detecting to Bacterial Concentration by Cultivation-Microscopy Method in the Oil Field Sewage

2013 ◽  
Vol 726-731 ◽  
pp. 2837-2844
Author(s):  
Shu Xia Guan ◽  
Hai Chun Ye ◽  
Xiao Xin Wang ◽  
Ying Ying Fan
Keyword(s):  
Serial Dilution ◽  
Oil Field ◽  
Dilution Method ◽  
Clear Water ◽  
Bacterial Concentration ◽  
Order Of Magnitude ◽  
Serial Dilution Method ◽  
Microscopy Method

Bacterial concentration is detected by the cultivation-microscopy method and the serial dilution method in the different oil field sewage. The results indicate that the results are the same when the bacterial concentration in the clear water, simulated water and waterflooded sewage were detected by the cultivation- microscopy method and the serial dilution method. The order of magnitude are the same and quotient are different when the bacterial concentration in the polymer- flooded sewage were detected by those. The bacterial concentration by the cultivation-microscopy method detected is more than by the serial dilution method detected when the waterflooded sewage and the polymer-flooded sewage were joined the biocide. They are used to detect the bacterial concentration in the different concentration scop of polymers, the results are the same in the concentration scop of polyacrylamide under 100mg/L, while the results are very different in the concentration scop of polyacrylamide above 200mg/L.

Direct Plating Versus Dilution Plating in Qualitatively Determining the Mold Flora of Dried Beans and Soybeans

1977 ◽  
Vol 60 (3) ◽  
pp. 741-743 ◽  
Author(s):  
Philip B Mislivec ◽  
Verneal R Bruce
Keyword(s):  
Relative Effectiveness ◽  
Serial Dilution ◽  
Individual Species ◽  
Dilution Method ◽  
Detection Rates ◽  
Direct Plating ◽  
Plating Method ◽  
Serial Dilution Method ◽  
Quantitative Results ◽  
Ground Soybean

Abstract Two methods presently used for examining whole foods and feeds for viable molds were evaluated for their relative effectiveness in the qualitative determination of the total number of mold species present in soybeans and dried beans: the direct plating method and the serial dilution method. Sixty-nine soybean samples and 40 dried bean samples were examined. Although the quantitative results of this study were inconclusive, the qualitative results show that the direct plating method was substantially more effective in detecting individual mold species. An average of 12.9 and 10.9 species was detected by the direct plating method in whole soybean and dried bean samples, respectively. An average of 4.4 and 2.8 species was detected by the dilution method in ground soybean and dried bean samples, respectively. A total of at least 37 mold species were found in the study, including 10 toxicogenic species. With few exceptions, detection rates of the 37 individual species were substantially greater among the samples examined by direct plating than those examined by serial dilution.

scholarly journals ON THE TITRATION OF BACTERIOPHAGE AND THE PARTICULATE HYPOTHESIS

1927 ◽  
Vol 11 (1) ◽  
pp. 71-81 ◽  
Author(s):  
Harry Clark
Keyword(s):  
Serial Dilution ◽  
Dilution Method ◽  
End Point ◽  
Serial Dilution Method

1. The theory of the serial dilution method of titration of bacterio-phage has been worked out on the basis of the simple particulate hypothesis. 2. It has been shown that, if the dilution constant is .1, only about 60 per cent of parallel runs on the same solution should give the same end-point, the average being taken over a great number of titrations of each of a great variety of solutions. 3. The discrepancy between this figure, 60 per cent, and Dr. Bronfenbrenner's estimate, 85 per cent, is considerable. 4. Inasmuch as the particulate hypothesis is well founded, no explanation of the discrepancy is suggested.

scholarly journals Lactate dehydrogenase of Mugil sp. (Mugilidae, Perciformes). Lack of electrokinetic, thermostability and kinetic differences among individuals with different number of scales

2000 ◽  
Vol 23 (1) ◽  
pp. 139-144
Author(s):  
Marcelo dos Santos ◽  
Maria Luiza B. Schwantes ◽  
Arno Rudi Schwantes
Keyword(s):  
Lactate Dehydrogenase ◽  
Thermal Inactivation ◽  
Electrophoretic Pattern ◽  
Competitive Inhibitor ◽  
Dilution Method ◽  
Substrate Affinity ◽  
Enzyme Substrate ◽  
Serial Dilution Method ◽  
Different Populations ◽  
Estuarine Region

The scale number in lateral sets (SNS) of Mugil sp. (Mugilidae, Perciformes) collected in the lagoon-estuarine region of Cananéia, State of São Paulo ranges from 33 to 39. Electrokinetic, kinetic and thermostability properties of lactate dehydrogenase (LDH) were tested to determine if individuals with different SNS correspond to different species or populations of mullet. As in many other teleosts, LDH-A*, LDH-B*, and LDH-C* loci were detected. Through a two-fold serial dilution method applied to 10 different tissues of Mugil sp., a bidirectionally divergent expression of these loci was suggested. No association among LDH electrophoretic pattern, thermal inactivation, kinetic responses and different SNS was observed. The apparent Km (pyr) values obtained here were similar to Km values obtained by other authors for muscle and heart LDH or their purified isoforms. The effect of NaCl on Km and Vmax values of Mugil sp. (35 and 39 SNS individuals) indicates that this salt behaves as a competitive inhibitor, since it decreases enzyme-substrate affinity. Thus, electrokinetic and thermostability behavior, Km and Vmax values and the effect of NaCl do not permit us to consider these mullets, with SNS ranging from 33 to 39, as belonging to different populations or species.

scholarly journals A serial dilution method for assessment of microplastic toxicity in suspension

2018 ◽  
Author(s):  
Zandra Gerdes ◽  
Markus Hermann ◽  
Martin Ogonowski ◽  
Elena Gorokhova
Keyword(s):  
Risk Assessment ◽  
Reference Material ◽  
Suspended Solids ◽  
Particulate Material ◽  
Serial Dilution ◽  
Dilution Method ◽  
Particle Exposure ◽  
Novel Approach ◽  
Serial Dilution Method ◽  
Global Concern

The occurrence of microplastic (MP) in the environment is of global concern. MP risk assessment, however, is currently hampered by lacking ecotoxicological methods due to conceptual and practical problems with particle exposure. Natural particles of similar size as MP, e.g., clay and cellulose, occur abundantly in the environment. For MP risk assessment and regulation it must be established whether the addition of MP to these particles represents an additional hazard. We present a novel approach employing a serial dilution of MP and reference particles, in mixtures, which allows the differentiation of MP effects from other particulates. We demonstrate the applicability of the method using an immobilisation test with Daphnia magna exposed to polyethylene terephthalate (MP) and kaolin clay (reference material). In the concentration range of 0.1 to 10000 mg L-1 of total suspended solids (TSS), with MP contributing 0-100 %, the LC50 values for MP-kaolin mixtures were significantly lower compared to the pure kaolin suspension. MP particles were thus more harmful to daphnids than the reference material. The estimated threshold for %MP contribution above which higher mortality was observed was 1 % MP at 36 mg TSS L-1. This approach has a potential for standardisation of MP ecotoxicological testing as well as other particulate material of anthropogenic origin.

scholarly journals POTENSI RIZOBAKTERI INDIGENUS SEBAGAI AGENS BIOKONTROL JAMUR FUSARIUM OXYSPORUM F. SP. CUBENSE PENYEBAB PENYAKIT LAYU TANAMAN PISANG

2020 ◽  
Vol 1 (1) ◽  
pp. 8-16
Author(s):  
Selviana Anggraini ◽  
Jumsu Trisno ◽  
Tizelia Tizelia
Keyword(s):  
Fusarium Oxysporum ◽  
Plant Disease ◽  
Culture Method ◽  
Serial Dilution ◽  
Dilution Method ◽  
Dual Culture ◽  
Test Results ◽  
Alternative Control ◽  
Serial Dilution Method ◽  
Physiological Characters

The use of rhizobacteria is an alternative control in suppressing plant disease attacks. The purpose of this study was to obtain indigenous rhizobacteria which have the potential as antagonist agents of Fusarium oxysporum f. sp. cubense. Sampling was carried out randomly selected on endemic lands. Isolation of rhizobacteria using serial dilution method and its antagonist test against Fusarium oxysporum f. sp. cubense uses the dual culture method. The isolation results from 3 banana cultivars (kepok, raja sere, and mas) obtained 24 rhizobacterial isolates with different morphological and physiological characters. The antagonist test results showed that 8 rhizobacterial isolates were able to suppress the growth of Fusarium oxysporum f. sp. cubense and is not plant pathogenic.

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