A CRITIQUE OF THE SERIAL DILUTION METHOD FOR QUANTITATIVE DETERMINATION OF BACTERIOPHAGE

Science ◽  
1932 ◽  
Vol 75 (1949) ◽  
pp. 494-495 ◽  
Author(s):  
A. P. Krueger
Keyword(s):  
Quantitative Determination ◽  
Serial Dilution ◽  
Dilution Method ◽  
Serial Dilution Method

scholarly journals ANTIBIOTICS RESISTANCE OF CORYNEBACTERIUM NON DIPHTHERIAE STRAINS

2017 ◽  
pp. 3-8
Author(s):  
G. G. Kharseeva ◽  
N. A. Voronina ◽  
T. D. Gasretova ◽  
O. I. Sylka ◽  
S. Yu. Tyukavkina
Keyword(s):  
Serial Dilution ◽  
Urogenital Tract ◽  
Antibiotics Resistance ◽  
Dilution Method ◽  
Frequency Of Occurrence ◽  
Serial Dilution Method ◽  
Resistant Strains

Aim. Study the frequency of occurrence of antibiotics resistant strains of various species of Corynebacterium non diptheriae. Materials and methods. C.pseudodiphtheriticum, C.pseudotuberculosis, C.xerosis, C.amycolatum, C.striatum, C.ulcerans strains isolated from patients with pathologies of respiratory and urogenital tract, as well as individuals taking prophylaxis examination were used. Sensitivity to antibacterial preparations was determined by the serial dilution method. Results. The highest number of Corynebacterium non diptheriae strains displayed resistance to benzylpenicillin (54.8%) and lincomycin (50.7%), and lowest - to cefotaxime, cefazolin (6.8%) and vancomycin (13.7%). The highest number of antibiotics resistant strains were detected among members of C.pseudotuberculosis {100%), C.xerosis (96.0%) and C. pseudodiphtheriticum (81.0%) species. Polyresistant strains were detected most frequently among C.xerosis, C.amycolatum and C.striatum species. Strains of Corynebacterium non diptheriae most frequently displayed resistance to 1 or 2 antibacterial preparations (24.7%), less frequently - to 3 (20.5%), 4 (13.7%), 5 (4.1%) and 6 (1.4%) preparations. Conclusion. The amount of antibiotics resistant strains of Corynebacterium non diptheriae is large (89.0%) and non-similar in various species.

Research on Detecting to Bacterial Concentration by Cultivation-Microscopy Method in the Oil Field Sewage

2013 ◽  
Vol 726-731 ◽  
pp. 2837-2844
Author(s):  
Shu Xia Guan ◽  
Hai Chun Ye ◽  
Xiao Xin Wang ◽  
Ying Ying Fan
Keyword(s):  
Serial Dilution ◽  
Oil Field ◽  
Dilution Method ◽  
Clear Water ◽  
Bacterial Concentration ◽  
Order Of Magnitude ◽  
Serial Dilution Method ◽  
Microscopy Method

Bacterial concentration is detected by the cultivation-microscopy method and the serial dilution method in the different oil field sewage. The results indicate that the results are the same when the bacterial concentration in the clear water, simulated water and waterflooded sewage were detected by the cultivation- microscopy method and the serial dilution method. The order of magnitude are the same and quotient are different when the bacterial concentration in the polymer- flooded sewage were detected by those. The bacterial concentration by the cultivation-microscopy method detected is more than by the serial dilution method detected when the waterflooded sewage and the polymer-flooded sewage were joined the biocide. They are used to detect the bacterial concentration in the different concentration scop of polymers, the results are the same in the concentration scop of polyacrylamide under 100mg/L, while the results are very different in the concentration scop of polyacrylamide above 200mg/L.

Direct Plating Versus Dilution Plating in Qualitatively Determining the Mold Flora of Dried Beans and Soybeans

1977 ◽  
Vol 60 (3) ◽  
pp. 741-743 ◽  
Author(s):  
Philip B Mislivec ◽  
Verneal R Bruce
Keyword(s):  
Relative Effectiveness ◽  
Serial Dilution ◽  
Individual Species ◽  
Dilution Method ◽  
Detection Rates ◽  
Direct Plating ◽  
Plating Method ◽  
Serial Dilution Method ◽  
Quantitative Results ◽  
Ground Soybean

Abstract Two methods presently used for examining whole foods and feeds for viable molds were evaluated for their relative effectiveness in the qualitative determination of the total number of mold species present in soybeans and dried beans: the direct plating method and the serial dilution method. Sixty-nine soybean samples and 40 dried bean samples were examined. Although the quantitative results of this study were inconclusive, the qualitative results show that the direct plating method was substantially more effective in detecting individual mold species. An average of 12.9 and 10.9 species was detected by the direct plating method in whole soybean and dried bean samples, respectively. An average of 4.4 and 2.8 species was detected by the dilution method in ground soybean and dried bean samples, respectively. A total of at least 37 mold species were found in the study, including 10 toxicogenic species. With few exceptions, detection rates of the 37 individual species were substantially greater among the samples examined by direct plating than those examined by serial dilution.

scholarly journals Quantitative and Qualitative Composition of Bacterial Communities of Malting Barley Grain and Malt during Long-Term Storage

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1301
Author(s):  
Soňa Felšöciová ◽  
Przemysław Łukasz Kowalczewski ◽  
Tomáš Krajčovič ◽  
Štefan Dráb ◽  
Miroslava Kačániová
Keyword(s):  
Quantitative Determination ◽  
Storage Period ◽  
Barley Grain ◽  
Coliform Bacteria ◽  
Dilution Method ◽  
Malting Barley ◽  
Long Term Storage ◽  
Plate Counts ◽  
Barley Grains

In the grain samples of three malting barley varieties harvested in 2016, and in the malt made from them, we observed microbial changes during storage. Analyses were performed after 0, 3, 6, and 9 months of storage in floor warehouses and silos. We monitored microbial changes based on the quantitative determination of microorganisms using a plate dilution method and qualitative determination of microorganisms, where we identified a particular species of bacteria using the MALDI-TOF MS Biotyper instrument (Bruker Daltonics, Bremen, Germany). Based on the quantitative determination of microorganisms, we found that the total plate counts of microorganisms was higher in malts than in barley grains. The storage period had the greatest impact on the number of coliform bacteria, which gradually increased with the growing months in storage. The number of lactic acid bacteria in barley grains with the growing months in storage and malt produced from them decreased slightly, and the content of the sporulating bacteria in all the samples fluctuated slightly. Differences in the numbers of observed microorganisms were negligible during storage between floor warehouses and silos. In the samples of barleys, the most commonly identified species were Pantoea agglomerans, which was also present in malt samples but did not belong to the dominant species. A diverse species representation appeared in the samples, whereas predominating species belonged to the Enterobacteriaceae family.

scholarly journals ON THE TITRATION OF BACTERIOPHAGE AND THE PARTICULATE HYPOTHESIS

1927 ◽  
Vol 11 (1) ◽  
pp. 71-81 ◽  
Author(s):  
Harry Clark
Keyword(s):  
Serial Dilution ◽  
Dilution Method ◽  
End Point ◽  
Serial Dilution Method

1. The theory of the serial dilution method of titration of bacterio-phage has been worked out on the basis of the simple particulate hypothesis. 2. It has been shown that, if the dilution constant is .1, only about 60 per cent of parallel runs on the same solution should give the same end-point, the average being taken over a great number of titrations of each of a great variety of solutions. 3. The discrepancy between this figure, 60 per cent, and Dr. Bronfenbrenner's estimate, 85 per cent, is considerable. 4. Inasmuch as the particulate hypothesis is well founded, no explanation of the discrepancy is suggested.

scholarly journals Genetic Study of Phenylthiocarbamide (PTC) Taste Sensitivity In Population of The Osing in Kemiren Village-Banyuwangi

2021 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Fenny Indriani ◽  
Rike Oktarianti ◽  
Syubbanul Wathon
Keyword(s):  
Genetic Study ◽  
Gene Frequency ◽  
Serial Dilution ◽  
Allele Frequencies ◽  
Genotype Frequency ◽  
Taste Sensitivity ◽  
Dilution Method ◽  
Serial Dilution Method ◽  
Different Populations ◽  
Age Range

The ability to taste phenylthiocarbamide (PTC), is autosomal trait inherited in a simple Mendelian recessive pattern. The frequency of Taster and non-Taster allele is varies in different populations. The purpose of the research is to investigate the prevalence, gene frequency and genotype frequency of taster (T) and non taster (ts of Osing population in Kemiren-Banyuwangi. PTC serial dilution method was used to assess the PTC Taster and non-Taster phenotypes. The Hardy–Weinberg method was used to determine allele frequencies. The total of samples were 227 people, male were 117 and female were 110 with age range of 15–30 years were randomly selected. The result showed that the Osing population as Taster were 210 (92,52%) and non Taster were 17samples (7,48%) . The allele frecuency of Taster (T) was 0,73 and non Taster (t) was 0,27 respectively. The genotype frequency of dominant Taster (TT) was 0,54, heterozygosity Taster (Tt) was 0,39, and genotype of non Taster (tt) was 0,07.

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