scholarly journals ANTIBIOTICS RESISTANCE OF CORYNEBACTERIUM NON DIPHTHERIAE STRAINS

2017 ◽  
pp. 3-8
Author(s):  
G. G. Kharseeva ◽  
N. A. Voronina ◽  
T. D. Gasretova ◽  
O. I. Sylka ◽  
S. Yu. Tyukavkina
Keyword(s):  
Serial Dilution ◽  
Urogenital Tract ◽  
Antibiotics Resistance ◽  
Dilution Method ◽  
Frequency Of Occurrence ◽  
Serial Dilution Method ◽  
Resistant Strains

Aim. Study the frequency of occurrence of antibiotics resistant strains of various species of Corynebacterium non diptheriae. Materials and methods. C.pseudodiphtheriticum, C.pseudotuberculosis, C.xerosis, C.amycolatum, C.striatum, C.ulcerans strains isolated from patients with pathologies of respiratory and urogenital tract, as well as individuals taking prophylaxis examination were used. Sensitivity to antibacterial preparations was determined by the serial dilution method. Results. The highest number of Corynebacterium non diptheriae strains displayed resistance to benzylpenicillin (54.8%) and lincomycin (50.7%), and lowest - to cefotaxime, cefazolin (6.8%) and vancomycin (13.7%). The highest number of antibiotics resistant strains were detected among members of C.pseudotuberculosis {100%), C.xerosis (96.0%) and C. pseudodiphtheriticum (81.0%) species. Polyresistant strains were detected most frequently among C.xerosis, C.amycolatum and C.striatum species. Strains of Corynebacterium non diptheriae most frequently displayed resistance to 1 or 2 antibacterial preparations (24.7%), less frequently - to 3 (20.5%), 4 (13.7%), 5 (4.1%) and 6 (1.4%) preparations. Conclusion. The amount of antibiotics resistant strains of Corynebacterium non diptheriae is large (89.0%) and non-similar in various species.

Research on Detecting to Bacterial Concentration by Cultivation-Microscopy Method in the Oil Field Sewage

2013 ◽  
Vol 726-731 ◽  
pp. 2837-2844
Author(s):  
Shu Xia Guan ◽  
Hai Chun Ye ◽  
Xiao Xin Wang ◽  
Ying Ying Fan
Keyword(s):  
Serial Dilution ◽  
Oil Field ◽  
Dilution Method ◽  
Clear Water ◽  
Bacterial Concentration ◽  
Order Of Magnitude ◽  
Serial Dilution Method ◽  
Microscopy Method

Bacterial concentration is detected by the cultivation-microscopy method and the serial dilution method in the different oil field sewage. The results indicate that the results are the same when the bacterial concentration in the clear water, simulated water and waterflooded sewage were detected by the cultivation- microscopy method and the serial dilution method. The order of magnitude are the same and quotient are different when the bacterial concentration in the polymer- flooded sewage were detected by those. The bacterial concentration by the cultivation-microscopy method detected is more than by the serial dilution method detected when the waterflooded sewage and the polymer-flooded sewage were joined the biocide. They are used to detect the bacterial concentration in the different concentration scop of polymers, the results are the same in the concentration scop of polyacrylamide under 100mg/L, while the results are very different in the concentration scop of polyacrylamide above 200mg/L.

Direct Plating Versus Dilution Plating in Qualitatively Determining the Mold Flora of Dried Beans and Soybeans

1977 ◽  
Vol 60 (3) ◽  
pp. 741-743 ◽  
Author(s):  
Philip B Mislivec ◽  
Verneal R Bruce
Keyword(s):  
Relative Effectiveness ◽  
Serial Dilution ◽  
Individual Species ◽  
Dilution Method ◽  
Detection Rates ◽  
Direct Plating ◽  
Plating Method ◽  
Serial Dilution Method ◽  
Quantitative Results ◽  
Ground Soybean

Abstract Two methods presently used for examining whole foods and feeds for viable molds were evaluated for their relative effectiveness in the qualitative determination of the total number of mold species present in soybeans and dried beans: the direct plating method and the serial dilution method. Sixty-nine soybean samples and 40 dried bean samples were examined. Although the quantitative results of this study were inconclusive, the qualitative results show that the direct plating method was substantially more effective in detecting individual mold species. An average of 12.9 and 10.9 species was detected by the direct plating method in whole soybean and dried bean samples, respectively. An average of 4.4 and 2.8 species was detected by the dilution method in ground soybean and dried bean samples, respectively. A total of at least 37 mold species were found in the study, including 10 toxicogenic species. With few exceptions, detection rates of the 37 individual species were substantially greater among the samples examined by direct plating than those examined by serial dilution.

scholarly journals TYPING OF VIBRIO CHOLERAE NON O1/NON O139 STRAINS, ISOLATED IN ROSTOV REGION IN 2014

2016 ◽  
pp. 3-9 ◽  
Author(s):  
N. A. Selyanskaya ◽  
I. V. Arkhangelskaya ◽  
A. S. Vodopianov ◽  
S. O. Vodopianov ◽  
V. D. Kruglikov ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Multiple Drug Resistance ◽  
Antibiotics Resistance ◽  
Dilution Method ◽  
Multiple Drug ◽  
Genetic Characteristics ◽  
Rostov Region ◽  
Serial Dilution Method ◽  
Water Transmission ◽  
Resistance Data

Aim. Comparative study of antibiotics resistance and VNTR-typing of Vibrio cholerae non O1/ non O139 strains, isolated on the territory of Rostov region in 2014. Materials and methods. Antibioticogramms of strains were determined by serial dilution method in dense nutrient medium according to MG 4.2.2495-09 (2009). Pheno-, sero- and VNTR-typing was carried out by conventional methods. Results. The studied strains belonged to V. cholerae species, did not agglutinate with O1 and O139 sera, were atoxigenic, hemolysis-positive, did not contain genes of cholera toxin and toxin-coregulating pili ofadhesion, contained genes ofhemagglutinin/protease, protease PrtV, collagenase, cytotonic factor Cef, outer membrane protein OmpW, tol- and vps-clusters, regulatory genes toxR and hapR. Antibioticogramms of the strains have shown the presence of cultures, resistant to ampicillin, ceftazidime, furazolidone, trimethoprim/sulfamethoxazole with intermediate resistance to streptomycin, kanamycin, gentamycin, amikacin, netilmicin. Approximately 20% of isolates had multiple drug resistance. Data of VNTR- and geno-typing confirmed a possibility of water transmission route of the infection. Conclusion. Execution of monitoring of cultures from environmental samples is necessary for timely detection of genetic characteristics, antibiotics resistance.

scholarly journals ON THE TITRATION OF BACTERIOPHAGE AND THE PARTICULATE HYPOTHESIS

1927 ◽  
Vol 11 (1) ◽  
pp. 71-81 ◽  
Author(s):  
Harry Clark
Keyword(s):  
Serial Dilution ◽  
Dilution Method ◽  
End Point ◽  
Serial Dilution Method

1. The theory of the serial dilution method of titration of bacterio-phage has been worked out on the basis of the simple particulate hypothesis. 2. It has been shown that, if the dilution constant is .1, only about 60 per cent of parallel runs on the same solution should give the same end-point, the average being taken over a great number of titrations of each of a great variety of solutions. 3. The discrepancy between this figure, 60 per cent, and Dr. Bronfenbrenner's estimate, 85 per cent, is considerable. 4. Inasmuch as the particulate hypothesis is well founded, no explanation of the discrepancy is suggested.

scholarly journals Genetic Study of Phenylthiocarbamide (PTC) Taste Sensitivity In Population of The Osing in Kemiren Village-Banyuwangi

2021 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Fenny Indriani ◽  
Rike Oktarianti ◽  
Syubbanul Wathon
Keyword(s):  
Genetic Study ◽  
Gene Frequency ◽  
Serial Dilution ◽  
Allele Frequencies ◽  
Genotype Frequency ◽  
Taste Sensitivity ◽  
Dilution Method ◽  
Serial Dilution Method ◽  
Different Populations ◽  
Age Range

The ability to taste phenylthiocarbamide (PTC), is autosomal trait inherited in a simple Mendelian recessive pattern. The frequency of Taster and non-Taster allele is varies in different populations. The purpose of the research is to investigate the prevalence, gene frequency and genotype frequency of taster (T) and non taster (ts of Osing population in Kemiren-Banyuwangi. PTC serial dilution method was used to assess the PTC Taster and non-Taster phenotypes. The Hardy–Weinberg method was used to determine allele frequencies. The total of samples were 227 people, male were 117 and female were 110 with age range of 15–30 years were randomly selected. The result showed that the Osing population as Taster were 210 (92,52%) and non Taster were 17samples (7,48%) . The allele frecuency of Taster (T) was 0,73 and non Taster (t) was 0,27 respectively. The genotype frequency of dominant Taster (TT) was 0,54, heterozygosity Taster (Tt) was 0,39, and genotype of non Taster (tt) was 0,07.

scholarly journals BIODIVERSITY AND ANTIBIOTICS RESISTANCE OF OPPORTUNISTIC PATHOGENIC ENTEROBACTERIA ISOLATED FROM SURFACE RESERVOIRS OF ROSTOV-ON-DON

2017 ◽  
pp. 17-23 ◽  
Author(s):  
A. V. Trishina ◽  
E. A. Bereznyak ◽  
I. R. Simonova ◽  
L. M. Verkina ◽  
A. Yu. Bereznyak ◽  
...  
Keyword(s):  
High Activity ◽  
Antibiotics Resistance ◽  
Dilution Method ◽  
Biochemical Tests ◽  
Maldi Biotyper ◽  
Serial Dilution Method ◽  
Antibacterial Preparation ◽  
Species Membership ◽  
Opportunistic Pathogenic

Aim. Study biodiversity, sensitivity/resistance to antibacterial preparation (ABP) of opportunistic microorganisms of Enterobacteriaceae family isolated from surface reservoirs of Rostov-on-Don in 2014 - 2016. Materials and methods. Determination of genus and species membership of enterobacteria was carried out by the results of a complex of biochemical tests using MALDI Biotyper program-apparatus unit. Sensitivity to ABP was determined by serial dilution method. Results. 468 strains of opportunistic and pathogenic enterobacteria were isolated during the study. 20 genera, 33 species of microorganisms were identified. (37.0%), Klebsiella (13.2%), Citrobacter (8.0%) predominated. 8.5% of the strains isolated in 2014 were sensitive to ABP, 5.2% - in 2015; strains sensitive to ABP were not detected in 2016. The number of polyresistant isolates has increased during the last 3 years: 40.7% strains in 2014,60.1 % in 2015. 80,0% in 2016. Ceftriaxone, gentamycin and ciprofloxacin had high activity against the isolated enterobacteria. The highest resistance was documented against ampicillin, nalidixic acid, nitro-furane, co-trimoxazole. Conclusion. The data obtained allow to evaluate the dynamics of increase of polyresistant strains of enterobacteria isolated from surface reservoirs.

scholarly journals A serial dilution method for assessment of microplastic toxicity in suspension

2018 ◽  
Author(s):  
Zandra Gerdes ◽  
Markus Hermann ◽  
Martin Ogonowski ◽  
Elena Gorokhova
Keyword(s):  
Risk Assessment ◽  
Reference Material ◽  
Suspended Solids ◽  
Particulate Material ◽  
Serial Dilution ◽  
Dilution Method ◽  
Particle Exposure ◽  
Novel Approach ◽  
Serial Dilution Method ◽  
Global Concern

The occurrence of microplastic (MP) in the environment is of global concern. MP risk assessment, however, is currently hampered by lacking ecotoxicological methods due to conceptual and practical problems with particle exposure. Natural particles of similar size as MP, e.g., clay and cellulose, occur abundantly in the environment. For MP risk assessment and regulation it must be established whether the addition of MP to these particles represents an additional hazard. We present a novel approach employing a serial dilution of MP and reference particles, in mixtures, which allows the differentiation of MP effects from other particulates. We demonstrate the applicability of the method using an immobilisation test with Daphnia magna exposed to polyethylene terephthalate (MP) and kaolin clay (reference material). In the concentration range of 0.1 to 10000 mg L-1 of total suspended solids (TSS), with MP contributing 0-100 %, the LC50 values for MP-kaolin mixtures were significantly lower compared to the pure kaolin suspension. MP particles were thus more harmful to daphnids than the reference material. The estimated threshold for %MP contribution above which higher mortality was observed was 1 % MP at 36 mg TSS L-1. This approach has a potential for standardisation of MP ecotoxicological testing as well as other particulate material of anthropogenic origin.

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