HOW COMMON IN POLISH POPULATION ARE GENOTYPES OF APOLIPOPROTEIN E WHICH PREDISPOSE TO ALZHEIMER’S DISEASE DEVELOPMENT?

Introduction Apolipoprotein E (ApoE) is a glycoprotein secreted mainly by hepatocytes. It’s involved in cell proliferation and cholesterol transport. ApoE is occurring primarily in 3 significant variants ε2, ε3 and ε4. It is coded by two genes what means the combination of two polymorphic alleles determines its genotype. ApoE-ε3/ε4 and -ε4/ε4 increase the risk of Alzheimer’s disease (AD) development several times compared to the general population. Early prevention can significantly slow down AD development and shorten its course. Aim The aim of this study was to check the distribution of ApoE gene among population from South Poland and compared results with previous study indicating allelic discrimination among random patients in research group. Material and methods In general we carried out genotyping polimorphism of ApoE. The process consisted of: 1. Colecting blood samples from patients, 2. Isolating DNA, 3. Preparating concentrations of DNA and checking it with spectrophotometer, 4. Allelic discrimination with fluorescent – labelled probes, 5. Genotyping of the ApoE polymorphism using Roche Lightcycler96 device. Study group consisted 830 subjects. 1660 determinations has been conducted. Results After expanding the research group by 81 patients it is shown that amount of carriers ApoE-ε2/ε4, ApoE-ε3/ε4 and ApoE-ε4/ε4 increases. The most numerous genotypes in research group are ApoE-ε2/ε2 and ApoE-ε3/ε3. Conclusions An upward trend in the relative numbers of carriers of genotype associated with a higher risk of developing AD has been noticed with the extension of research. Further research should be performed and the rese

Download Full-text

Apolipoprotein E gene and Alzheimer's disease: is tau the link?

Biochemical Society Symposium ◽

10.1042/bss0670111 ◽

2001 ◽

Vol 67 ◽

pp. 111-120 ◽

Cited By ~ 5

Author(s):

Simon Lovestone ◽

Brian Anderton ◽

Joanna Betts ◽

Rejith Dayanandan ◽

Graham Gibb ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Apolipoprotein E ◽

Significant Advance ◽

Cholesterol Transport ◽

Vascular Risk ◽

Polymorphic Variation ◽

Gene Encoding ◽

Single Mechanism ◽

Apolipoprotein E Gene

The finding that APOE (the gene encoding apolipoprotein E) polymorphic variation was associated with an altered risk of developing Alzheimer's disease (AD) was a significant advance and immediately prompted a search for the mechanisms responsible for this alteration. Some 6 years later, a number of different hypotheses remain that might account for this influence on pathogenesis with no single mechanism being unequivocally accepted. The different approaches to understanding these mechanisms can be broadly categorized as: those suggesting a remote effect, such as different rates of vascular risk factors in those with the different APOE alleles; those proposing altered neuronal vulnerability, perhaps due to apolipoprotein E (ApoE)-isoform-specific differences in local cholesterol transport; and those hypotheses postulating an ApoE interaction with the two key lesions of AD, plaques and tangles. In this chapter we will review the evidence for and against an interaction between ApoE and the neuronal cytoskeleton, in particular with the microtubule-associated protein tau.

Download Full-text