Evaluation of Root Extract of Cyphostemma Adenocaule (Vitaceae) for Antioxidant Activity, Brine Shrimp Toxicity, and Antiproliferative Activity against Hela Cervical Cancer Cells

Aim: To determine the phytochemicals in Ocimum gratissimum leaves, their phenolic content, antioxidant potential and antiproliferative activity against human prostate (DU145), colon (CT26) and cervical (HeLa 229) cancer cells. Place and Duration of the Study: Leaves of O. gratissimum were collected from cultivated plants in Wakiso district of Uganda. The samples were analyzed at Directorate of Government Analytical Laboratory, Kampala (Uganda) and Kenya Medical Research Institute, Centre for Traditional Medicine and Drug Research, Nairobi (Kenya) between August 2019 and January 2020. Methodology: The leaves were separately extracted by maceration using hexane, dichloromethane, ethyl acetate and methanol. The methanolic extract was further fractionated and subjected to solid phase extraction. Antiproliferative assay was done using dimethylthiazol-2,5-diphenyl-tetrazolium bromide assay while total phenolic content and antioxidant activity were determined by Folin-Ciocalteu method and 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay respectively. Compounds were identified by Fourier transform infrared spectroscopy and liquid chromatography-tandem mass spectrometry. Results: A total of 34 compounds were identified in the fractions. The highest mean total phenolic content was 401.07 ± 6.47 µg/ml for the methanolic extract which also had the highest antioxidant activity with minimum inhibitory concentration of 5.79 ± 0.13 mg/ml. There was a positive correlation between the antioxidant activity of the extracts and antiproliferative activity of the extracts on prostate and cervical cancer cell lines. The extracts exhibited the highest toxicity against prostate cancer cells and the least against cervical cancer cells. Conclusion: The results of this study support the traditional use of this plant in cancer therapy in Uganda. Further research should isolate pure anticancer compounds from this plant which could act as lead candidates in the development of anticancer drugs.

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Apoptotic, necrotic, and antiproliferative activity of diosgenin and diosgenin glycosides on cervical cancer cells

European Journal of Pharmacology ◽

10.1016/j.ejphar.2020.172942 ◽

2020 ◽

Vol 871 ◽

pp. 172942 ◽

Cited By ~ 2

Author(s):

José Misael Vicente Hernández-Vázquez ◽

Hugo López-Muñoz ◽

María Luisa Escobar-Sánchez ◽

Fernando Flores-Guzmán ◽

Benny Weiss-Steider ◽

...

Keyword(s):

Cervical Cancer ◽

Cancer Cells ◽

Antiproliferative Activity ◽

Cervical Cancer Cells

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Sideritis perfoliata inhibits cell proliferation, induces apoptosis and exhibits cellular antioxidant activity in cervical cancer cells

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas ◽

10.37360/blacpma.21.20.4.29 ◽

2021 ◽

Vol 20 (4) ◽

pp. 394-405

Author(s):

Gizem Cocelli ◽

◽

Mustafa Pehlivan ◽

Onder Yumrutas ◽

◽

...

Keyword(s):

Cervical Cancer ◽

Cell Proliferation ◽

Antioxidant Activity ◽

Cancer Cells ◽

Hela Cells ◽

Staining Method ◽

Antioxidant Activities ◽

Anticancer Activities ◽

Cellular Antioxidant Activity ◽

Cervical Cancer Cells

In this study, it was aimed to determine the antioxidant and anticancer activities of Sideritis perfoliata methanolic extract (SPE) on cervical cancer cells (HeLa). Different doses (25, 50, 100 and 200 µg/mL) of SPE were used to determine proliferation of HeLa cells by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) staining method. Induction of apoptosis was determined by Annexine-V and propidium iodide staining method. Interleukin (IL) 6-8 levels were measured by ELISA method. Antioxidant activities of SPE were determined by DPPH, DNA (plasmid pBR322) protecting and cellular antioxidant activity tests. Some phytochemicals of SPE were also screened by LC-MS-MS. It was determined that SPE reduced the proliferation of HeLa cells and also induced apoptosis. IL6-8 levels importantly decreased at 200 µg/mL. SPE exhibited moderately antioxidant activities in tests used. Among the phenolics identified, vanillic acid had the highest amount. As a result, it was determined to have the anticancer activity of SPE by decreasing cell proliferation, inducing apoptosis and decreasing IL6-8 in HeLa cells.

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