scholarly journals EXPRESSION OF HUMAN LEUKOCYTE ANTIGEN-E AND NATURAL KILLER CELLS IN INTRA UTERINE FETAL DEATH

2017 ◽  
Vol 53 (4) ◽  
pp. 252
Author(s):  
Sri Sulistyowati ◽  
Muhammad Anggit Nugroho ◽  
Supriyadi Hari Respati ◽  
Soetrisno Soetrisno
Keyword(s):  
Natural Killer Cells ◽  
Human Leukocyte Antigen ◽  
Nk Cells ◽  
Natural Killer ◽  
Fetal Death ◽  
Human Leukocyte ◽  
Normal Pregnancy ◽  
Mean Value ◽  
Leukocyte Antigen ◽  
The Mean

Intra Uterine Fetal Death (IUFD) is one contributor to infant mortality. Human Leukocyte Antigen-E (HLA-E) and Natural Killer Cells (NK cells) are believed to play an important role towards IUFD associated with immune maladaptation. This study aimed to determine the expression of HLA-E and NK cells on trophoblast on IUFD and normal pregnancy. The study used cross sectional approach undertaken at Department of Obstetrics and Gynecology, Dr. Moewardi Hospital, Surakarta, networking hospitals, and Anatomic Pathology Laboratory of Faculty of Medicine, Sebelas Maret University. The number of samples were 32 subjects consisting of 16 subjects with normal pregnancy and 16 subjects with IUFD. The expression of HLA-E and NK cells on trophoblast of subjects who met the inclusion and exclusion criteria were examined using immunohistochemistry method and t-test statistical analysis. The mean value of HLA-E expression in the trophoblast of IUFD group was 17.30±6.69, in normal pregnancy was 57.06±32.04, with p=0.00 (p<0.05). The mean value of NK cell expression in trophoblast in IUFD group was 78.62±36.43, in normal pregnancy was 19.87±6.43, with p=0.00 (p<0.05). This study concluded that the expression of HLA-E was lower and NK cells wass higher in IUFD compared to those in normal pregnancy.

scholarly journals Structural plasticity of KIR2DL2 and KIR2DL3 enables altered docking geometries atop HLA-C

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shoeib Moradi ◽  
Sanda Stankovic ◽  
Geraldine M. O’Connor ◽  
Phillip Pymm ◽  
Bruce J. MacLachlan ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
Human Leukocyte Antigen ◽  
Nk Cells ◽  
Natural Killer ◽  
Crystal Structures ◽  
Killer Cell ◽  
Human Leukocyte ◽  
Leukocyte Antigen ◽  
Functional Differences ◽  
Molecular Bases

AbstractThe closely related inhibitory killer-cell immunoglobulin-like receptors (KIR), KIR2DL2 and KIR2DL3, regulate the activation of natural killer cells (NK) by interacting with the human leukocyte antigen-C1 (HLA-C1) group of molecules. KIR2DL2, KIR2DL3 and HLA-C1 are highly polymorphic, with this variation being associated with differences in the onset and progression of some human diseases. However, the molecular bases underlying these associations remain unresolved. Here, we determined the crystal structures of KIR2DL2 and KIR2DL3 in complex with HLA-C*07:02 presenting a self-epitope. KIR2DL2 differed from KIR2DL3 in docking modality over HLA-C*07:02 that correlates with variabilty of recognition of HLA-C1 allotypes. Mutagenesis assays indicated differences in the mechanism of HLA-C1 allotype recognition by KIR2DL2 and KIR2DL3. Similarly, HLA-C1 allotypes differed markedly in their capacity to inhibit activation of primary NK cells. These functional differences derive, in part, from KIR2DS2 suggesting KIR2DL2 and KIR2DL3 binding geometries combine with other factors to distinguish HLA-C1 functional recognition.

scholarly journals Increased frequency of human leukocyte antigen–E inhibitory receptor CD94/NKG2A–expressing peritoneal natural killer cells in patients with endometriosis

2008 ◽  
Vol 89 (5) ◽  
pp. 1490-1496 ◽  
Author(s):  
Ricciarda Galandrini ◽  
Maria Grazia Porpora ◽  
Antonella Stoppacciaro ◽  
Federica Micucci ◽  
Cristina Capuano ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
Human Leukocyte Antigen ◽  
Natural Killer ◽  
Human Leukocyte ◽  
Inhibitory Receptor ◽  
Killer Cells ◽  
Leukocyte Antigen

scholarly journals Killer Cell Inhibitory Receptor Recognition of Human Leukocyte Antigen (HLA) Class I Blocks Formation of a pp36/PLC-γ Signaling Complex in Human Natural Killer (NK) Cells

1996 ◽  
Vol 184 (6) ◽  
pp. 2243-2250 ◽  
Author(s):  
Nicholas M. Valiante ◽  
Joseph H. Phillips ◽  
Lewis L. Lanier ◽  
Peter Parham
Keyword(s):  
Human Leukocyte Antigen ◽  
Nk Cells ◽  
Natural Killer ◽  
Nk Cell ◽  
Killer Cell ◽  
Human Leukocyte ◽  
Class I ◽  
Cell Cytotoxicity ◽  
Leukocyte Antigen ◽  
Nk Cell Cytotoxicity

The killer cell inhibitory receptors (KIR) of human natural killer (NK) cells recognize human leukocyte antigen class I molecules and inhibit NK cell cytotoxicity through their interaction with protein tyrosine phosphatases (PTP). Here, we report that KIR recognition of class I ligands inhibits distal signaling events and ultimately NK cell cytotoxicity by blocking the association of an adaptor protein (pp36) with phospholipase C-γ in NK cells. In addition, we demonstrate that pp36 can serve as a substrate in vitro for the KIR-associated PTP, PTP-1C (also called SHP-1), and that recognition of class I partially disrupts tyrosine phosphorylation of NK cell proteins, providing evidence for KIR-induced phosphatase activity.

scholarly journals Human Leukocyte Antigen E Contributes to Protect Tumor Cells from Lysis by Natural Killer Cells

Neoplasia ◽  
2011 ◽  
Vol 13 (9) ◽  
pp. 822-IN14 ◽  
Author(s):  
Elisa Lo Monaco ◽  
Elisa Tremante ◽  
Cristina Cerboni ◽  
Elisa Melucci ◽  
Leonardo Sibilio ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
Human Leukocyte Antigen ◽  
Natural Killer ◽  
Tumor Cells ◽  
Human Leukocyte ◽  
Killer Cells ◽  
Leukocyte Antigen

scholarly journals THE DIFFERENCE HUMAN LEUKOCYTE ANTIGEN-E (HLA-E) EXPRESSION AND NATURAL KILLER CELLS (NK CELLS) IN TROPHOBLAST BETWEEN INTRAUTERINE GROWTH RESTRICTION (IUGR) AND NORMAL PREGNANCY

2018 ◽  
Vol 3 (1) ◽  
pp. 15
Author(s):  
Soetrisno Soetrisno ◽  
Sri Sulistyowati ◽  
Supriyadi Hari Respati ◽  
Bambang Triono Cahyadi
Keyword(s):  
Human Leukocyte Antigen ◽  
Nk Cells ◽  
Intrauterine Growth Restriction ◽  
Human Leukocyte ◽  
Normal Pregnancy ◽  
Growth Restriction ◽  
Immunohistochemical Method ◽  
P Value ◽  
Leukocyte Antigen ◽  
Intrauterine Growth

Background: Intrauterine growth restriction (IUGR) is still a major cause of perinatal morbidity and mortality. IUGR incidence ranged from 4.4 to 6.44% of pregnant women. Human Leukocyte Antigen - E and NK cells are thought to play an important role towards the process of IUGR.Objective: To determine aetiopathogenesis HLA-E expression and NK Cell in IUGR.Methods: An analytical observational study conducted through a cross-sectional approach. The research was conducted on March-October 2015 at the Obstetrics and Gynecology Department, Dr. Moewardi Hospital Surakarta and at of Laboratory of Pathology, Faculty of Medicine, Universitas Sebelas Maret Surakarta. Subjects who meet the inclusion and exclusion criteria consisted of 20 patients with IUGR and 20 normal pregnant patient. The researcher examined HLA-E Expression and NK cells in trophoblast using immunohistochemical method and statistical analysis using t test.Results: The average HLA-E expression in the trophoblast in IUGR group 17.80 ± 5.55, compared with normal pregnancy 66.23 ± 19.83, p value = 0.0001 (p <0.05). The average expression of NK cells in trophoblast in IUGR group 137.71 ± 28.09 compared with normal pregnancy 31.75 ± 14.77, p value = 0.0001 (p <0.05).Conclusion: HLA-E expression in normal pregnancy trophoblast is higher than pregnancies with IUGR. Expression of NK cells in pregnancy with IUGR is higher compared with normal pregnancy.

scholarly journals Polymorphism in Human Cytomegalovirus UL40 Impacts on Recognition of Human Leukocyte Antigen-E (HLA-E) by Natural Killer Cells

2013 ◽  
Vol 288 (12) ◽  
pp. 8679-8690 ◽  
Author(s):  
Susan L. Heatley ◽  
Gabriella Pietra ◽  
Jie Lin ◽  
Jacqueline M. L. Widjaja ◽  
Christopher M. Harpur ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
Human Leukocyte Antigen ◽  
Natural Killer ◽  
Human Cytomegalovirus ◽  
Human Leukocyte ◽  
Killer Cells ◽  
Leukocyte Antigen
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