scholarly journals Brain Derived Neurotrophic Factor Levels in Aged Rats Post-Systemic Human Mesenchymal Stem Cell Administration

2019 ◽  
Vol 2 (2) ◽  
Author(s):  
Adisti Dwijayanti
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Intravenous Injection ◽  
Neurotrophic Factor ◽  
Human Mesenchymal Stem Cell ◽  
Brain Derived Neurotrophic Factor ◽  
Female Rats ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Aged Rats

Brain-Derived Neurotrophic Factor (BDNF) levels were affected by aging. Brain BDNF levels were known to decrease along with advanced age thus correlated with any diseases such as cognitive impairment and Alzheimer. Mesenchymal Stem Cell (MSC) is one of the potential modalities actively investigated against age-related diseases. This study evaluated the effect of human MSC administration to brain BDNF levels in aged rats. Intravenous injection of 10 million per body weight human MSC were given four times in 3 months interval to 22-24 months old female and male Spraque–Dawley rats. As control group, aged rats were injected by normal saline at the same volume and frequencies. Moreover, young 3-6 months rats also examined as negative control.  By the end of the experiment, we analyzed three rats from each group. Brain BDNF levels were measured by enzyme-linked immunosorbent assay and normalize to the protein levels. One-way ANOVA and LSD post hoc analysis was performed to compare the differences between groups. BDNF levels in male appeared similar between young, aged, and MSC treated groups. Meanwhile, control aged female groups had significantly lower BDNF levels compared to young (p = 0.019) and MSC-treated aged rats (p = 0.001). There was no difference of BDNF levels between young and MSC-treated aged in female rats (p = 0,068). Both sex had similar BDNF levels (p = 0.249) in control-aged groups. In contrast, female young and MSC-treated aged rats achieved significantly higher BDNF levels (p = 0.009 and p <0.001) compared to the male groups, respectively. These results suggest that human mesenchymal stem cell intravenous injection can increase brain BDNF levels in female aged rats.

Exercise training increases cardiac, hepatic and circulating levels of brain-derived neurotrophic factor and irisin in young and aged rats

2018 ◽  
Vol 36 (3) ◽  
Author(s):  
Muaz Belviranlı ◽  
Nilsel Okudan
Keyword(s):  
Exercise Training ◽  
Neurotrophic Factor ◽  
Wheel Running ◽  
Brain Derived Neurotrophic Factor ◽  
Female Rats ◽  
Enzyme Linked Immunosorbent Assay ◽  
Regular Exercise ◽  
Aged Rats ◽  
Sedentary Group ◽  
Circulating Levels

Abstract Background The objective of study was to examine the impacts of exercise training on cardiac, hepatic and plasma brain-derived neurotrophic factor (BDNF) and irisin levels in young and aged rats. Materials and methods Four-month-old (young) and 20-month-old (aged) female rats performed exercise training consisting of voluntary wheel running for 12 weeks. BDNF and irisin levels were analyzed in the heart, liver and plasma samples by using commercially available enzyme-linked immunosorbent assay (ELISA) kits. Results Cardiac, hepatic and plasma BDNF levels were lower in the aged sedentary rats, than in the young exercised and aged exercised rats (p < 0.05). Heart, liver and plasma irisin concentrations were lower in the aged sedentary group than in the young sedentary, young exercised and aged exercised groups (p < 0.05) and regular exercise increased irisin levels in all the analyzed tissues when compared to the sedentary counterparts (p < 0.05). Conclusions The current results show that regular exercise improves aging-induced decrease in the cardiac, hepatic and plasma BNDF and irisin levels.

scholarly journals Alginate-encapsulated brain-derived neurotrophic factor–overexpressing mesenchymal stem cells are a promising drug delivery system for protection of auditory neurons

2020 ◽  
Vol 11 ◽  
pp. 204173142091131 ◽  
Author(s):  
Jana Schwieger ◽  
Anika Hamm ◽  
Michael M. Gepp ◽  
André Schulz ◽  
Andrea Hoffmann ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cochlear Implant ◽  
Neurotrophic Factor ◽  
Spiral Ganglion ◽  
Brain Derived Neurotrophic Factor ◽  
Spiral Ganglion Neuron ◽  
Stem Cell Survival

The cochlear implant outcome is possibly improved by brain-derived neurotrophic factor treatment protecting spiral ganglion neurons. Implantation of genetically modified mesenchymal stem cells may enable the required long-term brain-derived neurotrophic factor administration. Encapsulation of mesenchymal stem cells in ultra-high viscous alginate may protect the mesenchymal stem cells from the recipient’s immune system and prevent their uncontrolled migration. Alginate stability and survival of mesenchymal stem cells in alginate were evaluated. Brain-derived neurotrophic factor production was measured and its protective effect was analyzed in dissociated rat spiral ganglion neuron co-culture. Since the cochlear implant is an active electrode, alginate–mesenchymal stem cell samples were electrically stimulated and alginate stability and mesenchymal stem cell survival were investigated. Stability of ultra-high viscous-alginate and alginate–mesenchymal stem cells was proven. Brain-derived neurotrophic factor production was detectable and spiral ganglion neuron survival, bipolar morphology, and neurite outgrowth were increased. Moderate electrical stimulation did not affect the mesenchymal stem cell survival and their viability was good within the investigated time frame. Local drug delivery by ultra-high viscous-alginate-encapsulated brain-derived neurotrophic factor–overexpressing mesenchymal stem cells is a promising strategy to improve the cochlear implant outcome.

Serum and Aqueous Humor Levels of Brain-Derived Neurotrophic factor in Patients with Primary Open-Angle Glaucoma and Normal Tension Glaucoma

2021 ◽  
Author(s):  
Yu Wan Cha ◽  
Seong Taeck Kim
Keyword(s):  
Aqueous Humor ◽  
Neurotrophic Factor ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Brain Derived Neurotrophic Factor ◽  
Normal Tension Glaucoma ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Open Angle ◽  
Serum Bdnf

Abstract PurposeThis study designed to compare the levels of brain-derived neurotrophic factor (BDNF) in the serum and aqueous humor (AH) of patients with primary open-angle glaucoma (POAG) and normal-tension glaucoma (NTG).MethodsThis prospective, observational study consists of 30 patients with POAG, 30 patients with NTG, and 30 healthy controls. The serum and AH BDNF levels were assessed using an enzyme-linked immunosorbent assay.ResultsBDNF levels in serum and AH were markedly lower in the glaucoma groups (POAG and NTG) than in the control group (p < 0.05). When comparing the NTG and POAG groups, the average serum BDNF level was significantly lower in the NTG group than in the POAG group (p < 0.05). The difference in the mean BDNF levels in AH between the POAG and NTG groups was not statistically significant. (p = 0.538).ConclusionWe confirmed that serum BDNF levels were lower in patients with NTG than in those with POAG. BDNF could be a causative systemic biomarker in NTG.

Brain-derived neurotrophic factor facilitates maturation of mesenchymal stem cell-derived dopamine progenitors to functional neurons

2009 ◽  
Vol 110 (3) ◽  
pp. 1058-1069 ◽  
Author(s):  
Katarzyna A. Trzaska ◽  
Cecile C. King ◽  
Ke-Yong Li ◽  
Eldo V. Kuzhikandathil ◽  
Martha C. Nowycky ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Neurotrophic Factor ◽  
Brain Derived Neurotrophic Factor

Brain-derived neurotrophic factor in non-proliferative diabetic retinopathy with diabetic macular edema

2020 ◽  
pp. 112067212094480
Author(s):  
Yong Hyun Jun ◽  
Seong Taeck Kim
Keyword(s):  
Diabetic Retinopathy ◽  
Macular Edema ◽  
Diabetic Macular Edema ◽  
Proliferative Diabetic Retinopathy ◽  
Neurotrophic Factor ◽  
Serum Levels ◽  
Brain Derived Neurotrophic Factor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
The Mean

Purpose: To investigate aqueous humor (AH) and serum levels of brain-derived neurotrophic factor (BDNF) in non-proliferative diabetic retinopathy (NPDR) patients with diabetic macular edema (DME). Methods: The prospective study consists of 20 patients with DME NPDR, 20 patients with no-DME NPDR, and 20 healthy control subjects. Serum and AH samples were obtained during cataract surgery and intravitreal injection. Serum and AH levels of BDNF were measured by enzyme-linked immunosorbent assay. Results: The mean serum levels of BDNF were lower in both NPDR groups compared to the control group (DME NPDR group, p = 0.015; no-DME NPDR group, p = 0.024). Furthermore, the mean serum level of BDNF was lower in the DME NPDR group compared to the no-DME NPDR group ( p = 0.041). The mean AH levels of BDNF were significantly reduced in both NPDR groups compared to the control group (DME NPDR group, p < 0.001; no-DME NPDR group, p = 0.006). Further, the mean AH level of BDNF was significantly lower in the DME NPDR group compared to the no-DME NPDR group ( p = 0.037). Conclusion: Serum and AH levels of BDNF were reduced in NPDR patients with DME than without DME.

scholarly journals Physical Exercise Increased Brain-Derived Neurotrophic Factor in Elderly Population with Depression

2019 ◽  
Vol 7 (13) ◽  
pp. 2057-2061 ◽  
Author(s):  
Fauziah Nuraini Kurdi ◽  
Rostika Flora
Keyword(s):  
Physical Exercise ◽  
Neurotrophic Factor ◽  
Elderly Population ◽  
Elderly Women ◽  
Brain Derived Neurotrophic Factor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Bdnf Level ◽  
Depression Group ◽  
Depressive Episodes

BACKGROUND: Depression is very common in the elderly population. Physical exercise is one of the non-pharmacological procedures that promise to be a solution to improve the severity of depression. Brain-Derived Neurotrophic Factor (BDNF) plays a role in maintaining the survival of neuronal cells and in the regulation of synapse plasticity, affecting serotonin production in the hippocampus and thus the depressive symptoms. AIM: This study aimed to assess the role of physical exercise in affecting BDNF levels in elderly with depression. METHODS: Thirty-five elderly women (age ≥ 50 years) with depressive episodes based on Diagnostic and Statistical Manual of Mental Disorders (DSM)-V criteria were enrolled as treatment group, and 35 elderly women without depressive episodes were enrolled as control group, and underwent physical exercise in the form of treadmill with a speed of 6 km/h for 15 minutes. Physical exercise was carried out once a day for 28 days. As much as 1 ml of blood from the study, subjects were obtained from the cubital vein before the exercise commenced. Brain-Derived Neurotrophic Factor (BDNF) serum level was assessed by Enzyme-Linked Immunosorbent Assay (ELISA). Data were presented in the form of mean ± SD. An independent T-test was used to test levels after exercise in the depression group compared to the non-depression group. RESULTS: Pre-exercise BDNF levels in the depression group were lower than the group of elderly without depression. Physical exercise increased BDNF production in both elderly groups with and without depression. In the depression group, the increasing percentage of BDNF level was higher compared to non-depressive elderly. CONCLUSION: The increasing percentage of BDNF level was found to be higher in depressive elderly performing physical exercise. Physical exercise may be beneficial in supporting the therapy of elderly with depression.

Sign in / Sign up

Export Citation Format

Share Document