scholarly journals Biochemical Differentiation of Pseudomonas fluorescens of Assam Soil and their Utility in Management of Bacterial Wilt of Solanaceous Crops

2017 ◽  
Vol 6 (6) ◽  
pp. 2796-2806
Author(s):  
Thalhun L. Kipgen ◽  
L.C. Bora
Keyword(s):  
Pseudomonas Fluorescens ◽  
Bacterial Wilt ◽  
Biochemical Differentiation

scholarly journals Pemanfaatan Bacillus subtilis dan Pseudomonas fluorescens dalam pengendalian hayati Ralstonia solanacearum penyebab penyakit layu bakteri pada tomat

Jurnal Agro ◽  
10.15575/2305 ◽  
2018 ◽  
Vol 5 (1) ◽  
pp. 1-12
Author(s):  
Istiqomah Istiqomah ◽  
Dian Eka Kusumawati
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Wilt Disease ◽  
Total Phenol ◽  
Tomato Production ◽  
Bacterial Wilt Disease ◽  
Type Test ◽  
Important Disease

Salah satu penyakit penting pada produksi tomat di Indonesia adalah layu bakteri yang disebabkan oleh Ralstonia solanacearum. Alternatif untuk mengendalikan penyakit layu bakteri adalah dengan menggunakan Bacillus subtilis dan Pseudomonas fluorescens. Tujuan penelitian ini untuk mengetahui kemampuan B. subtilis dan P. fluorescens dalam mengendalikan penyakit layu bakteri yang disebabkan R. solanacearum serta mekanisme penghambatannya. Penelitian ini terdiri dari 5 tahap, yaitu perbanyakan inokulum R. solanacearum, uji virulensi dan uji hipersensitif  R. solanacearum, uji antagonis B. subtilis dan P. fluorescens terhadap R. solanacearum pada media agar, uji jenis antibiosis, penelitian di rumah kaca, dan analisis total fenol. Hasil penelitian uji antagonis menunjukkan bahwa semua isolat B. subtilis dan P. fluorescens memiliki potensi menghambat R. solanacearum dengan tipe antibiosis bakteriostatik. Hasil analisis kadar fenol menunjukkan bahwa terjadi peningkatan total fenol secara signifikan pada tanaman tomat yang diaplikasikan isolat B. subtilis UB-ABS6, P. fluorescens UB-PF5 dan P. fluorescens UB-PF6. Penelitian di rumah kaca menunjukkan bahwa semua tanaman tomat yang diaplikasikan agens hayati mengalami penundaan masa inkubasi dibandingkan dengan kontrol. Isolat B. subtilis UB-ABS2, B. subtilis UB-ABS6, P. fluorescens UB-PF5 dan P. fluorescens UB-PF6 secara signifikan menekan kejadian penyakit layu bakteri berturut-turut 50%, 30%, 60%, dan 60%. B. subtilis dan P. fluorescens dapat dimanfaatkan untuk mengendalikan layu bakteri pada tomat yang disebabkan oleh Ralstonia solanacearum. One of important disease that infects tomato production in Indonesia is bacterial wilt disease caused by Ralstonia solanacearum. Alternative on controlling bacterial wilt is using Bacillus subtilis and Pseudomonas fluorescens. Goal of the research was to find out ability of B. subtilis and P. fluorescens to control R. Solanacearum and mechanism of the inhibition. This research divided into 5 stages, i.e. propagation of R. solanacearum, virulence and hypersensitive tests of R. Solanacearum, antagonist test of B. subtilis and P. fluorescens against R. solanacearum on agar medium, antibiosis type test, research in greenhouse, and total phenol analysis. The result showed that all isolates of B. subtilis and P. fluorescens have potential to inhibite R. solanacearum by bacteriostatic antibiosis type. The total phenol level showed significant increase of phenol on tomato along with the application of isolates B. subtilis UB-ABS6, P. fluorescens UB-PF5 and P. fluorescens UB-PF6. Research in the greenhouse showed that all tomatoes, which had been given bioagent, did delay on the incubation than the control. Isolates of B. subtilis UB-ABS2, B. subtilis UB-ABS6, P. fluorescens UB-PF5, and P. fluorescens UB-PF6 had significantly inhibited the bacterial wilt disease 50%, 30%, 60%, and 60%, respectively. Therefore, B. subtilis and P. fluorescens can be used to control bacterial wilt diseases on tomato caused by Ralstonia solanacearum.

Bacterial wilt of tomato in Karnataka and its management by Pseudomonas fluorescens

BioControl ◽  
2009 ◽  
Vol 54 (5) ◽  
pp. 685-695 ◽  
Author(s):  
S. C. Vanitha ◽  
S. R. Niranjana ◽  
C. N. Mortensen ◽  
S. Umesha
Keyword(s):  
Pseudomonas Fluorescens ◽  
Bacterial Wilt

scholarly journals Evaluation of Trichoderma spp., Pseudomonas fluorescens and Bacillus subtilis for biological control of Ralstonia wilt of tomato

F1000Research ◽  
2018 ◽  
Vol 6 ◽  
pp. 2028 ◽  
Author(s):  
Shiva Yendyo ◽  
Ramesh G.C. ◽  
Binayak Raj Pandey
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Bacterial Wilt ◽  
Defense Mechanism ◽  
Plant Root ◽  
Infected Plant ◽  
Biocontrol Agents ◽  
Digital Microscopy ◽  
Trichoderma Spp ◽  
Combined Application

Background:Ralstoniaspp. is a major pathogenic microbe for tomato, which invades the roots of diverse plant hosts and colonizes xylem vessels causing wilt, especially in tropical, subtropical and warm-temperate regions.Ralstoniaspp.produces several virulence factors helping it to invade the plant’s natural defense mechanism. Native isolates ofTrichoderma spp., Pseudomonas fluorescensandBacillus subtiliscan be used as biocontrol agents to control the bacterial wilt and combined application of these beneficial microbes can give better results.Methods:Bacterial wilt infection in the field was identified by field experts and the infected plant part was used to isolateRalstoniaspp.in CPG media and was positively identified. Subsequently, the efficacy of the biocontrol agents was tested and documented using agar well diffusion technique and digital microscopy. 2ml of the microbial concentrate (109cells/ml) was mixed in one liter of water and was applied in the plant root at the rate of 100 ml per plant as a treatment method.Results:It was observed that the isolatedTrichodermaspp. AA2 andPseudomonas fluorescensPFS were most potent in inhibiting the growth ofRalstoniaspp.,showing ZOI 20.67 mm and 22.33 mm, respectively. Digital microscopy showed distinct inhibitory effect on the growth and survival ofRalstoniaspp.The results from the field data indicated thatTrichodermaspp. andPseudomonas fluorescensalone were able to prevent 92% and 96% of the infection and combination of both were more effective, preventing 97% of infection. Chemical control methods prevented 94% of infection.Bacillus subtiliscould only prevent 84 % of the infection.  Conclusions:Antagonistic effect againstRalstonia spp.shown by native isolates ofTrichodermaspp. andP. fluorescensmanifested the promising potential as biocontrol agents. Combined application gave better results. Results shown byBacillus subtiliswere not significant.

Effect of Pseudomonas fluorescens in Controlling Bacterial Wilt of Tomato

2002 ◽  
Vol 1 (2) ◽  
pp. 71-73 ◽  
Author(s):  
K.M. Khalequzza ◽  
M.A. Jinnah . ◽  
M.A.A.M. Rashid . ◽  
M.N.A. Chowdhury . ◽  
Md. Masud Alam .
Keyword(s):  
Pseudomonas Fluorescens ◽  
Bacterial Wilt

scholarly journals Evaluation of Trichoderma spp., Pseudomonas fluorescens and Bacillus subtilis for biological control of Ralstonia wilt of tomato

F1000Research ◽  
2018 ◽  
Vol 6 ◽  
pp. 2028 ◽  
Author(s):  
Shiva Yendyo ◽  
Ramesh G.C. ◽  
Binayak Raj Pandey
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Bacterial Wilt ◽  
Defense Mechanism ◽  
Plant Root ◽  
Infected Plant ◽  
Biocontrol Agents ◽  
Digital Microscopy ◽  
Trichoderma Spp ◽  
Combined Application

Background:Ralstoniaspp. is a major pathogenic microbe for tomato, which invades the roots of diverse plant hosts and colonizes xylem vessels causing wilt, especially in tropical, subtropical and warm-temperate regions.Ralstoniaspp.produces several virulence factors helping it to invade the plant’s natural defense mechanism. Native isolates ofTrichoderma spp., Pseudomonas fluorescensandBacillus subtiliscan be used as biocontrol agents to control the bacterial wilt and combined application of these beneficial microbes can give better results.Methods:Bacterial wilt infection in the field was identified by field experts and the infected plant part was used to isolateRalstoniaspp.in CPG media and was positively identified. Subsequently, the efficacy of the biocontrol agents was tested and documented using agar well diffusion technique and digital microscopy. 2ml of the microbial concentrate (109cells/ml) was mixed in one liter of water and was applied in the plant root at the rate of 100 ml per plant as a treatment method.Results:It was observed that the isolatedTrichodermaspp. AA2 andPseudomonas fluorescensPFS were most potent in inhibiting the growth ofRalstoniaspp.,showing ZOI 20.67 mm and 22.33 mm, respectively. Digital microscopy showed distinct inhibitory effect on the growth and survival ofRalstoniaspp.The results from the field data indicated thatTrichodermaspp. andPseudomonas fluorescensalone were able to prevent 92% and 96% of the infection and combination of both were more effective, preventing 97% of infection. Chemical control methods prevented 94% of infection.Bacillus subtiliscould only prevent 84 % of the infection.  Conclusions:Antagonistic effect againstRalstonia spp.shown by native isolates ofTrichodermaspp. andP. fluorescensmanifested the promising potential as biocontrol agents. Combined application gave better results. Results shown byBacillus subtiliswere not significant.

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