Identification and Detection of Maize Seed Borne Pathogens  using Different Seed Testing Methods

A comparison of the Ulster, New Zealand, and Ottawa methods of seed examination for the presence of Polyspora lini Laff., the cause of stem-break and browning disease of flax, showed great variation in the percentage of infection obtained, depending upon the method used. The Ulster method, in which the seed is not treated before it is plated, does not differentiate deep-seated infection from superficial infection of the seed, but has proved satisfactory in actual practice with seed samples that are not contaminated seriously by saprophytic fungi. The New Zealand method, by which surface contamination is supposedly removed by washing the seed in running water, has not been found reliable. The Ottawa method, by which the seed is surface sterilized before it is plated, does not indicate the degree of superficial infection, but does give the percentage of deep-seated infection, and hence it indicates the suitability of the seed for planting purposes if the seed is treated with a fungicide. It is suggested that a centrifuge test combined with the Ottawa plating method would provide a better index of the health condition of flax seed as far as disease caused by. P. lini is concerned.

Download Full-text

Seed Testing Methods

Encyclopedia of Plant and Crop Science ◽

10.1081/e-epcs-120021918 ◽

2004 ◽

pp. 1-4

Author(s):

Daniel G. Curry

Keyword(s):

Testing Methods ◽

Seed Testing

Download Full-text

A model of a successful utilization of a high genetic potential of maize yield

Genetika ◽

10.2298/gensr0802191p ◽

2008 ◽

Vol 40 (2) ◽

pp. 191-203

Author(s):

Milovan Pavlov ◽

Goran Saratlic ◽

Zivorad Videnovic ◽

Zoran Stanisic

Keyword(s):

Seed Production ◽

Maize Yield ◽

Maize Seed ◽

Maize Breeding ◽

Seed Testing ◽

Seed Processing ◽

Foreign Countries ◽

Processing Plants ◽

Production Organization ◽

Maize Research Institute

The principle of a system, defined as a ZP system, implying corresponding relationship among research, seed production and seed marketing, is that each segment within the system has its tasks and responsibilities, as well as, a clear interest. This system was established at the Maize Research Institute, Zemun Polje, almost half a century ago. The crucial characteristic is that this system encompasses obtained results of scientific accomplishments (patent - a released hybrid), optimal utilisation of the environmental conditions, facilities for seed drying, processing and packing, staff and transport capacities. The ZP system provides the economic interest of all participants in studies and the maize seed production. The fundamental base of the quality seed production within the ZP system is a multidisciplinary programme on maize breeding, as well as, 535 released hybrids with standard and specific traits. According to regulations in foreign countries, approximately 100 ZP maize hybrids have been released abroad. Agroecological conditions in Serbia are favorable for the development of the best genotypes and the production of basic and certified maize seed. There 10 processing plants that apply recent technologies in the maize seed processing procedure. Several generations of experts have been trained and gained experience within the maize seed production. Three seed testing laboratories have been accredited by the International Seed Testing Association. According to regulations in Serbia, monitoring of seed production under field conditions, and further on, during the processing practice is done only by designate authorities. This study presents one of successful systems of the seed production organization applicable in countries with similar conditions.

Download Full-text

An improved method of seed germination testing in Kabuli chickpea

Indian Journal of Agricultural Research ◽

10.18805/ijare.a-4879 ◽

2018 ◽

Author(s):

Basave Gowda ◽

A. Krishna Naik ◽

Rakesh . ◽

C. Mathad ◽

B.S. Ganiger ◽

...

Keyword(s):

Seed Germination ◽

Germination Percentage ◽

Improved Method ◽

Testing Methods ◽

Seedling Vigour ◽

Seed Testing ◽

Testing Method ◽

Vigour Index ◽

Paper Method ◽

Germinated Seeds

An experiment was conducted during 2014-2015 to standardize the seed testing method in Kabuli chickpea MNK-1 by number of seeds and number of germination papers. Among the different testing methods 25 seeds per replication recorded highest seed germination percentage (91.58%), less number of fresh un-germinated seeds (1.91%), abnormal seedlings (4.00%), diseased seeds (2.33%) and significantly higher seedling vigour index-I and II (2215 and 6863), among the number of germination papers used 2+1 as in between paper method(Bottom 2 and one paper above) recorded significantly higher germination percentage (92.12%), less number of fresh un-germinated seeds (1.41%), abnormal seedlings (3.66%), diseased seeds (2.33%) and significantly higher seedling vigour index-I and II (2463 and 6900), when compared to other. Irrespective of number of germination paper and seeds used however, interaction of 25 seeds per replication and 2+1 between paper method of seed germination testing showed significantly germination percentage (96.50%), less number of fresh un-germinated seeds (0.00%), abnormal seedlings (1.00%), diseased seeds (1.00%) and significantly higher seedling vigour index-I and II (2844 and 7792) followed by 50 seeds per replication.

Download Full-text