Comparison of Laminoplasty vs. Laminectomy for Cervical Spondylotic Myelopathy: A Systematic Review and Meta-Analysis

ObjectivesLaminoplasty (LP) and laminectomy (LC) with or without fusion are recommended as treatment procedures for cervical spondylotic myelopathy (CSM). The purpose of this study is to conduct a meta-analysis to analyze the results of CSM patients undergoing LP or LC surgery.MethodsWe systematically and comprehensively searched Web of Science, Cochrane Library, PubMed, EMBASE, OVID, VIP database, Google Scholar, Chinese Bio-medicine Literature database, and China Scientific Journal Full-text database to July 2021 for randomized controlled trials (RCTs) and observational case series that compared LP and LC in patients with CSM. The main endpoints were the surgical process, radiographic outcomes, clinical outcomes, and surgical complications.ResultsA total of 19 were included the inclusion criteria in this meta-analysis (n = 4,348 patients). There was no significant difference in range of motion (ROM), sagittal vertical axis (SVA), Japanese Orthopedic Association (JOA), Cobb angle, visual analog scale (VAS), cervical curvature index (CCI), Nurick score, Neck Dysfunction Index (NDI), and complications. LP was found to be superior than LC in terms of complications of C5 radiculopathy and surperficial infection.ConclusionOur results indicate that LP can achieve better results in C5 radiculopathy and superficial infection in surgical treatment of CSM compared with LC. Further high-quality research is warranted to further verify our findings.Systematic Review RegistrationPRISMA: CRD42018107070.

An Assessment of 3.5mm Reconstruction Plate Fixation in Olecranon Fractures

Background: Olecranon process is a large, curved eminence comprising of the proximal and posterior part of the ulna. It lies subcutaneously which makes it more vulnerable to injury. Due to intra-articular extension of fractures, anatomical reduction and early mobilization should be achieved in every case and usually managed surgically. Aims and Objectives: To access the results of reconstruction plate in fracture olecranon.Materials &Methods: This was a prospective study consisted of 25 cases of olecranon fractures which were managed by open reduction and internal fixation using 3.5mm reconstruction plate. Patients were followed up every month till 6 months. At each follow up visit clinical and radiological parameters were assessed: Final assessment was done at 6 months using the Mayo Elbow Performance Score.Result: According to the AO classification, Type A-1 – 7 cases, A-3 – 1case, B-1 – 13 cases, B-3 – 1 case, C-1 – 1 case, C-2 – 1 case, C-3 – 1 case. An adequate reduction was maintained in all fractured olecranon until union. Average radiological union time was 12 weeks in 72% cases, 15 weeks in 16% cases, 18 weeks in 8% cases and > 18 weeks in 4% cases. The results were graded as per the criteria laid by Rogers et al as excellent in 84% cases, good in 12% and unsatisfactory in 4% cases. 2 cases developed superficial infection and 1 deep infection and 1 delayed union.Conclusion: Open reduction and internal fixation of fracture of olecranon with 3.5mm reconstruction plate is based on sound biomechanical principle with a good functional outcome and a low incidence of complications.

First report of tomato fruit blotch virus infecting tomatoes in Brazil

Recently, a new blunervirus was reported in tomatoes showing fruit chlorotic lesions. This virus, named tomato fruit blotch virus (ToFBV), was found associated with the tomato fruit blotch disease in Italy and Australia, even though Koch’s postulates were not fulfilled and no viral particles were seen in leaf dips observed with an electron microscope (Ciuffo et al. 2020). In December 2019, symptoms of circular or irregular chlorotic blotches were observed in tomato fruits in an organic farm in Distrito Federal, Brazil. Five different tomato cultivars (2100 plants of cv. Sweet grape, 1700 of Giacomo, 560 of Grazianni, 160 of Tropical, and 160 of DRC 5640) were being grown in two greenhouses and all of them presented the symptoms in at least one fruit, particularly in older fruits. No virus-like symptoms were observed in young and middle leaves, but older leaves could not be examined because they were removed as a routine activity of the farm; and also due to the moderate infestation of the tomato russet mite Aculops lycopersici, associated with leaf and stem necrosis. No viral particles were observed in an electron microscope analysis of symptomatic fruit tissues, and sap inoculation and grafting of stems did not produce any symptom in indicator plants. Two young and asymptomatic plants with the first fruits still in development were removed from another greenhouse of the farm and transported to our greenhouse, but the typical blotch symptoms neither appeared in the fruits nor the necrosis symptoms in the leaves. Serological tests performed for all collected leaf and fruit samples using antibodies produced in-house against common tomato-infecting tospoviruses and potyviruses were negative, as well as a polymerase chain reaction (PCR) detection test for begomoviruses (Rojas et al. 1993). Total RNA from newly collected samples consisting of one symptomatic fruit sample and five asymptomatic leaf samples from distinct plants were individually extracted using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and pooled for next generation sequencing (NGS). The library was constructed using TruSeq Stranded Total RNA with Ribo-Zero Plant (Illumina, San Diego, USA) and sequenced at Macrogen, Inc. (Seoul, South Korea) in an Illumina Novaseq6000 platform. The 4,621,977,958 reads obtained were trimmed using Trimommatic 0.35 (Bolger et al. 2014) and contigs were assembled using Velvet (Zerbino and Birney 2008). Following tblastx analysis on Geneious 9.1.8 (Biomatters Ltd.) and BLAST on the NCBI platform (Altschul et al. 1990), seven contigs matching tomato chlorosis virus (ToCV) and five contigs matching ToFBV were identified. Sequences for each of the four genome components of ToFBV (MK517477-MK517480) already present in databases were used as reference using the Map to Reference function in Geneious. A total of 338,402, 78,039, 555,302 and 461,474 reads mapped to virus genome components 1 to 4, respectively, with >99% coverage for each. Four final consensus sequences were used for BLAST analyses on NCBI and presented 97 to 99.7 % nucleotide identity with those used for mapping. These sequences were deposited in GenBank as isolate MAL under accession numbers MW546267 (RNA 1, 5770 nt), MW546268 (RNA 2, 3612 nt), MW546269 (RNA 3, 2826 nt) and MW546270 (RNA 4, 1950 nt). The primer pair Bluner1F (5’-ATTCCTGTTCCTTCGGATAAACTCGT-3’) and Bluner1R (5’-CACACGTGCAGGAAATGGAAAGA-3’) directed to RNA 1 was used to specifically detect the virus. Three leaf samples and two fruit samples, each from a different plant with typical symptoms, were tested positive for ToFBV and negative using ToCV-specific primers in RT-PCR (Dovas et al. 2002). This confirmed that although some plants pooled in the HTS library were infected with ToCV, the chlorotic blotch symptom was clearly associated with the presence of ToFBV. Furthermore, the ~0.5 kbp amplicon for ToFBV-specific primers from one randomly selected sample was sequenced with both primers and the resulting sequence shared 100% nt identity with the RNA 1 of ToFBV isolate Fondi2018 from Italy (MK517477). Then, the virus was detected in the tissue from the surface of another fruit, but not from its internal part, suggesting a superficial infection. The findings presented here are of high phytosanitary significance, given the strong symptoms associated with tomato fruit blotch disease and the identification of ToFBV in the tomato samples from Brazil.

Staples versus sutures for skin closure in hip arthroplasty: a meta-analysis and systematic review

Objective The purpose of the present study was to estimate complications and other outcomes associated with staple and suture closure after hip arthroplasty through meta-analysis techniques and a systematic review. Methods We searched for articles in EMBASE, PubMed, Medline, Web of Science and the Cochrane Library. To determine the eligibility of the searched trials, Cochrane Collaboration's Review Manager software was used to perform the meta-analysis. Results Five randomized controlled trials and one retrospective cohort trial were included in our study. Our study indicated that for skin closure after hip arthroplasty, the risks of superficial infection and prolonged discharge were higher with staples than with sutures. There was no significant difference between the two groups in terms of allergic reaction, dehiscence, inflammation, abscess formation, the Hollander Wound Evaluation Scale or patient's satisfaction with skin closure methods. However, suturing required a longer operating time. Conclusions Closure with sutures is associated with lower risks of superficial infection and prolonged discharge than closure with staples following hip arthroplasty, but it may take more time.

Management of subtrochanteric femur fractures: is open reduction associated with poor outcomes?

Purpose The aim of this study was to identify factors associated with the need for open reduction in subtrochanteric femoral fractures and investigate the effect of cerclage wiring compared to open reduction alone, on the development of complications, especially infection and non-union. Methods All consecutive patients with a fracture involving the subtrochanteric region were retrospectively identified, over an 8-year period. Data documented and analysed included patient demographics, fracture characteristics, patient comorbidities, time to fracture union and development of complications. Results A total of 512 patients met the inclusion criteria (523 fractures). Open reduction was performed in 48% (247) of the fractures. Following matching and regression analysis, we identified diaphyseal extension of the fracture to be associated with an open reduction (OR: 2.30; 95% CI 1.45–3.65; p < 0.001). Open reduction was also associated with an increased risk of superficial infection (OR: 7.88; 95% CI 1.63–38.16; p = 0.010), transfusion within 48 h following surgery (OR: 2.44; 95% CI 1.96–4.87; p < 0.001) and a prolonged surgical time (OR: 3.09; 95% CI 1.96–4.87; p < 0.001). The risk of non-union, deep infection and overall mortality was not increased with open reduction. The use of cerclage wires [50 out of 201 fractures (24.9%) treated with an open reduction] to achieve anatomical reduction as compared to open reduction alone significantly reduced the risk of non-union (OR: 0.20; 95% CI 0.06–0.74; p = 0.015). Conclusion Open reduction of subtrochanteric fractures is not associated with an increased risk of deep infection and non-union, even though it is associated with an increased risk of superficial infection, prolonged surgical time and transfusion. The use of cerclage wire is associated with reduced risk of non-union with little evidence of an increase in complications. Level of evidence III.

Ankle fusion with tibiotalocalcaneal retrograde nail for fragility ankle fractures: outcomes at a major trauma centre

Purpose Fragility ankles fractures in the geriatric population are challenging to manage, due to fracture instability, soft tissue compromise, and patient co-morbidities. Traditional management options include open reduction internal fixation, or conservative treatment, both of which are fraught with high complication rates. We aimed to present functional outcomes of elderly patients with fragility ankle fractures treated with retrograde ankle fusion nails. Methods A retrospective observational study was performed on patients who underwent intramedullary nailing with a tibiotalocalcaneal nail. Twenty patients met the inclusion criteria of being over sixty and having multiple co-morbidities. Patient demographics, AO/OTA fracture classification, intra-operative and post-operative complications, time to mobilisation and union, AOFAS and Olerud-Molander scores, and patient mobility were recorded. Results There were seven males and thirteen females, with a mean age of 77.82 years old, five of whom are type 2 diabetics. Thirteen patients returned to their pre-operative mobility state, and the average Charlson Co-morbidity Index (CCI) was 5.05. Patients with a low CCI are more likely to return to pre-operative mobility status (p = 0.16; OR = 4.00). All patients achieved radiographical union, taking on average between 92.5 days and 144.6 days. The mean post-operative AOFAS and Olerud-Molander scores were 53.0 and 50.9, respectively. There were four cases of superficial infection, four cases of broken or loose distal locking screws. There were no deep infections, periprosthetic fractures, nail breakages, or non-unions. Conclusion Tibiotalocalcaneal nailing is an effective and safe option for managing unstable ankle fractures in the elderly. This technique leads to lower complication rates and earlier mobilisation than traditional fixation methods.

Evaluation of Antimicrobial Activity of Conyza bonariensis Leaf Extracts against Clinically Isolated Fungi Causing Superficial Infection

Plants have been used since long time ago to treat infectious diseases and are considered as important sources of new antimicrobial agents. In this study, crude extracts from leaves of Conyza bonariensis were prepared using methanol, ethyl acetate, hexane, and chloroform. Antimicrobial activity of the extracts was evaluated against pathogenic fungi causing superficial infection (Candida albicans, Malassezia globosa, and Malassezia furfur). Results demonstrated that all extracts had different effects against all the tested fungi with the exception of crude extract using hexane which did not show any effect against M. furfur. A strong effect was observed with chloroform and hexane extracts on C. albicans (32.60 ± 4.69 mm and 27.00 ± 1.00 mm), respectively. While, ethyl acetate and methanol extracts showed the best effect against M. furfur (30.80 ± 1.71 mm and 27.00 ± 1.00 mm), respectively. Moreover, the ethyl acetate showed a considerable effect on M. globosa (25.03 ± 1.05 mm). Minimum inhibitory concentration (MIC) of the fractions was also determined by the microbroth dilution method. The results recorded as the MIC values of the tested extracts against fungi varied from 0.19 ± 0.00 to 66.66 ± 2.86 mg/mL. Ethyl acetate was the best and powerful extract with the lowest MIC value of 0.190.19 ± 0.00 mg/mL for all tested fungi followed by chloroform and methanol extracts with the MIC values ranging from 0.19 ± 0.00 to 0.78 ± 0.00 mg/mL and 0.84 ± 0.68 to 1.56 mg/mL, respectively. Concerning minimum bactericidal concentration (MFC), ethyl acetate was the most potent extract with a MFC value of 0.190.19 ± 0.00 mg/mL for C. albicans and M. furfur. Higher (0.39 mg/mL) MFC was recorded against M. globosa by this extract. In conclusion, solvent extracts of some C. bonariensis can be used to treat infections with pathogenic fungi such as C. albicans, M. furfur, and M. globosa. Further studies should consider this plant as one of the best candidates for the discovery of potent antimicrobial compounds that treat superficial infections.

Lateral Trans-Malleolar Approach for Posterior Malleolar Fractures of the Ankle for Articular and Syndesmosis Reduction : A Retrospective Study

Background The lateral transmalleolar approach (LTA) is a surgical approach for posterior malleolar fractures (PMFs), providing direct visualization of the articular surface of the posterior malleolus, though temporarily disrupting the syndesmosis. However, it is still unclear whether this fibrous joint can be effectively reconstructed afterward to prevent complications. We report the clinical results and computed tomography (CT) findings for articular and syndesmosis reduction status after surgery with LTA for PMF. Methods Sixteen patients who underwent PMF reduction with LTA and were followed up for at least 1 year were evaluated retrospectively. According to the AO/OTA classification, the fracture type was type 44B in 13 patients and 44C in three patients. The Haraguchi classifications of PMF were type 1 in six cases and type 2 in 10 cases. The American Orthopedic Foot and Ankle Society Ankle-Hindfoot Scale (AOFAS) score, post-surgical complications, and reduction status of PMF and syndesmosis on CT were investigated. Results The mean AOFAS score was 93.0 ± 5.2 points. Post-operative complications were observed in one case of superficial infection and one case of delayed fibular union. The articular step-off seen on CT improved significantly after surgery (5.9 ± 2.9 mm preoperatively vs. 0.6 ± 0.8 mm post-operatively). Syndesmosis malreduction was seen in three cases, including two high fibular fractures and one comminuted fibular fracture with osteoporosis. Conclusions The LTA provided favourable clinical results and effective reduction of intra-articular PMFs. However, surgeons should be aware of the possibility of syndesmosis malreduction in cases of high fibular fractures or comminuted fibular fractures.

Study to Determine the Outcomes of Closed Tibial Shaft Fractures Treated with Reamed Inter Locking Nails

Aim: The aim of the study is to evaluate the results of treatment of a closed reamed interlocking in the treatment of closed fractures of the tibial shaft. Study design: A Descriptive observational study Venue and Duration: This study was conducted in the Orthopedic department of Ayub Teaching Hospital, Abbottabad for six months duration i.e from September 2020 to February 2021. Patients and methods: The study included 43 patients over 19 years of age. The patients were taken to the emergency and Orthopedic department for surgery of Ayub Teaching Hospital, Abbottabad for six months duration i.e from September 2020 to February 2021. Written approvals were granted from all selectees. Detailed history was asked and each patient was assessed clinically and radiographically. The reamed intramedullary nailing performed according to the protocol. Patients were followed for 1 year and assessed for infection, union, knee and ankle range of motion, and implant problems. 3 patients were excluded from follow-up, and the study was held in 40 patients. Results: All fractures were having complete union without the need for bone grafting. Simple fractures have union at mean of 14 weeks, comminuted and segmented fractures have union for longer than five weeks, and a mean duration of 18.5 weeks. Superficial infection at the screw site occurred in two patients, and the drainage was done, antibiotics were given and distal screw were removed, respectively. While all patients had a full range of knee motion, 37 patients had a full range of ankle movement. The other three patients had a 15-to-20-degree dorsiflexion loss at the ankle joint. No nail fracture, proximal screw end fracture in one patients and distal screw fracture in two patients were observed. Conclusion: We came to the conclusion that closed intramedullary nailing in the case of a closed shaft fracture of the tibia is a safe and satisfactory technique with a high rate of union and a relatively low complication rate and early return to activity. Keywords: Fracture, Internal, Fixation, Interlocking nail.

Management Algorithm of External Fixation in Lower Leg Arterial Injury for Limb Salvages

Purpose: The purpose of this study was to review the roles of using external fixation to rescue the patients who sustained arterial injuries in the lower legs.Methods: Demographics, surgical treatment and outcomes in 88 patients with lower leg arterial injuries treated by external fixation at two trauma centers from 2009 to 2018 were reviewed. The primary outcome was the rate of successful lower leg salvage, while secondary outcomes were complications and functional recovery.Results:80 patients (90 legs) maintained a successful lower leg salvage. The patients were followed up for an average of 15.5±5.5 months. 6 patients (8 pins) experienced pin-tract infection, pins loosening happened in 2 patients (4 pins), 7 patients (7 legs) developed wound superficial infection, 3 patients (3 legs) with a deep infection developed osteomyelitis, 16 patients (17 legs) suffered the bone nonunion or bone defect. The average healing time of fracture was 5.6±4.3months. The maintain of external fixation average time was 5.8±3.6 months. Conclusion: With correctly judging the condition of limb ischemia, mastering reasonably the operation indications, and preventing complications, good clinical effects can be achieved when external fixation is used.Level of evidence: Retrospective cohort, level IV.