Formulation of Alternative and Cost Effective Growth Medium for Beneficial Microorganisms using Post Biomethanated Distillery Spentwash

AbstractThe bacteriumZymomonas mobilisnaturally produces ethanol at near theoretical maximum yields, making it of interest for industrial ethanol production.Z. mobilisrequires the vitamin pantothenate for growth. Here we characterized the genetic basis for theZ. mobilispantothenate auxotrophy. We found that this auxotrophy is due to the absence of a single gene,panD, encoding aspartate-decarboxylase. Heterologous expression ofEscherichia coliPanD inZ. mobilisor supplementation of the growth medium with the product of PanD activity, β-alanine, eliminated the need for exogenous pantothenate. We also determined that IlvC, an enzyme better known for branched-chain amino acid synthesis, is required for pantothenate synthesis inZ. mobilis, as it compensates for the absence of PanE, another pantothenate synthesis pathway enzyme. In addition to contributing to an understanding of the nutritional requirements ofZ. mobilis, our results have led to the design of a more cost-effective growth medium.

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Adaptation of Mycobacterium smegmatis to an Industrial Scale Medium and Isolation of the Mycobacterial PorinMspA

The Open Microbiology Journal ◽

10.2174/1874285801307010092 ◽

2013 ◽

Vol 7 (1) ◽

pp. 92-98 ◽

Cited By ~ 3

Author(s):

Sebastian O Wendel ◽

Ayomi S Perera ◽

Peter H Pfromm ◽

Peter Czermak ◽

Stefan H Bossmann

Keyword(s):

Growth Medium ◽

Mycobacterium Smegmatis ◽

Large Scale ◽

Culture Medium ◽

Cell Mass ◽

Cost Effective ◽

Scale Production ◽

Large Scale Production ◽

Simple Growth ◽

Effective Growth

The adaptation of the organism to a simple and cost-effective growth medium is mandatory in developing a process for large scale production of the octamericporinMspA, which is isolated fromMycobacterium smegmatis. A fermentation optimization with the minimal nutrients required for growth has been performed. During the fermentation, the iron- and ammonium chloride concentrations in the medium were varied to determine their impact on the observed growth rates and cell mass yields. Common antibiotics to control contamination were eliminated in favor of copper sulfate to reduce costs. MspA has been successfully isolated from the harvestedM. smegmatisusingaqueous nOPOE (n-octyloligooxyethylene) at 65°C. Because of the extraordinary stability of MspA, it is possible to denature and precipitate virtually all other proteins and contaminants by following this approach. To further purify the product, acetone is used for precipitation. Gel electrophoresis confirmed the presence and purity of MspA. A maximum of 840µg (via Bradford assay) of pure MspA per liter of the optimized simple growth medium has been obtained. This is a 40% increase with respect to the previously reported culture medium for MspA.

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EVALUATION OF SOIL PARAMETERS AND OPTIMIZATION OF A COST EFFECTIVE GROWTH CONDITIONS FOR ARABIDOPSIS THALIANA.

International Journal of Advanced Research ◽

10.21474/ijar01/5489 ◽

2017 ◽

Vol 5 (9) ◽

pp. 1642-1651 ◽

Cited By ~ 1

Author(s):

Abhishek Kumar ◽

◽

Madhusmita Panigrahy ◽

KishoreCS Panigrahi ◽

◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Cost Effective ◽

Growth Conditions ◽

Soil Parameters ◽

Effective Growth

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COST EFFECTIVE ALTERNATIVES FOR DISPOSAL OF DISTILLERY SPENTWASH WITH A VIEW TO ACHIEVE ZERO DISCHARGE

Proceedings of the Water Environment Federation ◽

10.2175/193864705783865901 ◽

2005 ◽

Vol 2005 (12) ◽

pp. 4093-4101

Author(s):

Mantha Nagaraj ◽

Arvind Kumar

Keyword(s):

Cost Effective ◽

Zero Discharge ◽

Distillery Spentwash

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Training Agency Staff through Peer Group Supervision

Social Casework ◽

10.1177/104438948606700705 ◽

1986 ◽

Vol 67 (7) ◽

pp. 418-423 ◽

Cited By ~ 5

Author(s):

Jerry L. Marks ◽

Donna F. Hixon

Keyword(s):

Quality Of Service ◽

Child Welfare ◽

Peer Group ◽

Cost Effective ◽

Group Supervision ◽

Training Method ◽

Protective Services ◽

Agency Staff ◽

Effective Growth

Peer group supervision implemented in a public child welfare agency enhanced the training of staff in the management and treatment of protective services cases in a cost-effective, growth-producing way. The agency wanted a training method that would upgrade the overall quality of service.

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Facile and cost-effective growth of MoS2 on 3D porous graphene-coated Ni foam for robust and stable hydrogen evolution reaction

Journal of Alloys and Compounds ◽

10.1016/j.jallcom.2019.02.192 ◽

2019 ◽

Vol 788 ◽

pp. 267-276 ◽

Cited By ~ 13

Author(s):

Sajjad Hussain ◽

Dhanasekaran Vikraman ◽

Linh Truong ◽

Kamran Akbar ◽

Iqra Rabani ◽

...

Keyword(s):

Hydrogen Evolution ◽

Hydrogen Evolution Reaction ◽

Cost Effective ◽

Ni Foam ◽

Porous Graphene ◽

Effective Growth

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Optimization of an effective growth medium for biomass production of Bacillus cereus

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/869/1/012009 ◽

2021 ◽

Vol 869 (1) ◽

pp. 012009

Author(s):

F Feliatra ◽

U M Batubara ◽

I Effendi ◽

A Adelina

Keyword(s):

Bacillus Cereus ◽

Growth Medium ◽

Biomass Production ◽

Cell Mass ◽

Liquid Waste ◽

Skim Milk ◽

Plate Count ◽

Cell Protein ◽

Fish Feed ◽

Effective Growth

Abstract Protein is the main source of nutrition in fish feed. It is necessary to use bacterial single-cell protein (SCPs) as a substitute for conventional protein. This study aims to determine the effectiveness of tofu liquid waste as a growth medium for biomass production of Bacillus cereus as an SCPs. The isolates used were B. cereus SN7 and B. cereus consortium (combination of 5 isolates of B. cereus with different strains). The method used was experimental where isolates were cultured in a medium containing tofu liquid waste with different concentrations (8%, 10%, and 12%) enriched with 10% skim milk. Furthermore, the analysis of an effective growth medium was carried out by three methods, namely total plate count, optical density, and cell-dry weight. Cell biomass measurements were carried out for 24 hours with an observation time of every 6 hours. The results showed the best growth medium used in the culture of B. cereus SN7 and B. cereus consortium was tofu liquid waste at a concentration of 12% enriched with 10% skim milk. In conclusion, the cell mass of B. cereus SN7 and B. cereus consortium significantly increased at 6 to 12 hours.

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Utilization of inland saline water for Spirulina cultivation

Journal of Water Reuse and Desalination ◽

10.2166/wrd.2013.102 ◽

2013 ◽

Vol 3 (4) ◽

pp. 346-356 ◽

Cited By ~ 4

Author(s):

K. P. Sandeep ◽

S. P. Shukla ◽

V. Harikrishna ◽

A. P. Muralidhar ◽

A. Vennila ◽

...

Keyword(s):

Chlorophyll A ◽

Growth Medium ◽

Spirulina Platensis ◽

Saline Water ◽

Value Added ◽

Cost Effective ◽

Downstream Processing ◽

Step Process ◽

Indoor And Outdoor

An attempt was made to assess the suitability of the inland saline water of Rohtak (Haryana) for mass cultivation of Spirulina platensis, a salt loving cyanobacterium. Cultivation of S. platensis was performed in indoor and outdoor culture units. The investigation revealed that the yield of biomass in de-calcified inland saline water was comparable to the yield obtained in synthetic chemical-based prescribed growth medium. Further, the quality of biomass in terms of protein, chlorophyll a, carotenoids and phycocyanin contents was also comparable to the prescribed medium. The downstream processing of the biomass through a three-step process resulted in an appreciable quantity of a highly valuable pigment, phycocyanin (purity ratio: A620/A280 = 3.13–3.39). The overall observations of the study suggest that inland saline water can be used for cost-effective production of Spirulina biomass and value-added chemicals. The removal of calcium and salts from inland saline water by S. platensis cultivation also offers an added advantage for the reuse of the spent medium for agricultural and aquacultural purposes.

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