Gross Morphological Studies on Portal Vein in  Adult Indian Sheep (Ovis aries)

The main products of intestinal hydrolysis of dietary triglycerides are free fatty acids and monoglycerides. These form micelles from which the lipids are absorbed across the mucosal cell brush border. Biochemical studies have indicated that intestinal mucosal cells possess a triglyceride synthesising system, which uses monoglyceride directly as an acylacceptor as well as the system found in other tissues in which alphaglycerophosphate is the acylacceptor. The former pathway is used preferentially for the resynthesis of triglyceride from absorbed lipid, while the latter is used mainly for phospholipid synthesis. Both lipids are incorporated into chylomicrons. Morphological studies have shown that during fat absorption there is an initial appearance of fat droplets within the cisternae of the smooth endoplasmic reticulum and that these subsequently accumulate in the golgi elements from which they are released at the lateral borders of the cell as chylomicrons.We have recently developed several methods for the fine structural localization of acyltransferases dependent on the precipitation, in an electron dense form, of CoA released during the transfer of the acyl group to an acceptor, and have now applied these methods to a study of the fine structural localization of the enzymes involved in chylomicron lipid biosynthesis. These methods are based on the reduction of ferricyanide ions by the free SH group of CoA.

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Chromic Acid Etching of Polyethylene

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100070801 ◽

1973 ◽

Vol 31 ◽

pp. 72-73

Author(s):

J. D. Muzzy ◽

R. D. Hester ◽

J. L. Hubbard

Keyword(s):

Chromic Acid ◽

Low Cost ◽

Acid Etching ◽

Crystalline Morphology ◽

Bulk Specimen ◽

Morphological Studies ◽

Perfect Form ◽

Molecular Length ◽

Lamellar Texture ◽

Cost Studies

Polyethylene is one of the most important plastics produced today because of its good physical properties, ease of fabrication and low cost. Studies to improve the properties of polyethylene are leading to an understanding of its crystalline morphology. Polyethylene crystallized by evaporation from dilute solutions consists of thin crystals called lamellae. The polyethylene molecules are parallel to the thickness of the lamellae and are folded since the thickness of the lamellae is much less than the molecular length. This lamellar texture persists in less perfect form in polyethylene crystallized from the melt.Morphological studies of melt crystallized polyethylene have been limited due to the difficulty of isolating the microstructure from the bulk specimen without destroying or deforming it.

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Biological and Morphological Studies on Low-Leukemia-Strain Mice with Hodgkin's-Like Neoplasia Induced by Serial Cell-Free Transmission of Leukemic Organs of SJL/J Strain Mice

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100100792 ◽

1968 ◽

Vol 26 ◽

pp. 210-211

Author(s):

S. Fujinaga ◽

K. Maruyama ◽

C.W. Williams ◽

K. Sekhri ◽

L. Dmochowski

Keyword(s):

Tissue Culture ◽

Type A ◽

Reticulum Cell ◽

Type B ◽

Viral Origin ◽

Serial Transfer ◽

Strain Mouse ◽

Morphological Studies ◽

Cell Neoplasm ◽

Free Material

Yumoto and Dmochowski (Cancer Res.27, 2098 (1967)) reported the presence of mature and immature type C leukemia virus particles in leukemic organs and tissues such as lymph nodes, spleen, thymus, liver, and kidneys of SJL/J strain mice with Hodgki's-like disease or reticulum cell neoplasm (type B). In an attempt to ascertain the possibility that this neoplasia may be of viral origin, experiments with induction and transmission of this neoplasm were carried out using cell-free extracts of leukemic organs from an SJL/J strain mouse with spontaneous disease.It has been possible to induce the disease in low-leukemia BALB/c and C3HZB strain mice and serially transfer the neoplasia by cell-free extracts of leukemic organs of these mice. Histological examination revealed the neoplasia to be of either reticulum cell-type A or type B. Serial transfer is now in its fifth passage. In addition leukemic spleen from another SJL/J strain mouse with spontaneous reticulum cell neoplasm (type A) was set up in tissue culture and is now in its 141st serial passage in vitro. Preliminary results indicate that cell-free material of 39th tissue culture passage can reproduce neoplasia in BALB/c mice.

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Morphological studies of linear (AB)n multiblock copolymers and their blends

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100122320 ◽

1992 ◽

Vol 50 (1) ◽

pp. 384-385

Author(s):

Richard J. Spontak ◽

Steven D. Smith ◽

Arman Ashraf

Keyword(s):

Electron Microscopy ◽

Microphase Separation ◽

Multiblock Copolymers ◽

First Order ◽

Morphological Studies ◽

Transmission Electron ◽

First Order Phase Transition ◽

Covalently Bonded ◽

Total Molecular Weight ◽

First Order Phase

Block copolymers are composed of sequences of dissimilar chemical moieties covalently bonded together. If the block lengths of each component are sufficiently long and the blocks are thermodynamically incompatible, these materials are capable of undergoing microphase separation, a weak first-order phase transition which results in the formation of an ordered microstructural network. Most efforts designed to elucidate the phase and configurational behavior in these copolymers have focused on the simple AB and ABA designs. Few studies have thus far targeted the perfectly-alternating multiblock (AB)n architecture. In this work, two series of neat (AB)n copolymers have been synthesized from styrene and isoprene monomers at a composition of 50 wt% polystyrene (PS). In Set I, the total molecular weight is held constant while the number of AB block pairs (n) is increased from one to four (which results in shorter blocks). Set II consists of materials in which the block lengths are held constant and n is varied again from one to four (which results in longer chains). Transmission electron microscopy (TEM) has been employed here to investigate the morphologies and phase behavior of these materials and their blends.

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An ultrastructural study on the shark liver

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010016011x ◽

1990 ◽

Vol 48 (3) ◽

pp. 512-513

Author(s):

Masako Yamada ◽

Yutaka Tanuma

Keyword(s):

Portal Vein ◽

Kupffer Cells ◽

Ultrastructural Study ◽

Lipid Droplets ◽

Microscopic Level ◽

The Other ◽

Fish Stock ◽

Structural Studies ◽

Light Microscopic Level ◽

Perfusion Fixation

Although many fine structural studies on the vertebrate liver have been reported on mammals, avians, reptiles, amphibians, teleosts and cyclostomes, there are no studies on elasmobranchii liver except one by T. Ito etal. (1962) who studied it on light microscopic level. The purpose of the present study was to as certain the ultrastructural details and cytochemical characteristics of normal elasmobranchii liver and was to compare with the other higher vertebrate ones.Seventeen Scyliorhinus torazame, one kind of elasmobranchii, were obtained from the fish stock of the Ueno Zoo aquarium, Ueno, Tokyo. The sharks weighing about 300-600g were anesthetized with MS-222 (Sigma), and the livers were fixed by perfusion fixation via the portal vein according to the procedure of Y. Saito et al. (1980) for 10 min. Then the liver tissues were immersed in the same fixative for 2 hours and postfixed with 1% OsO4-solution in 0.1 Mc acodylate buffer for one hour. In order to make sure a phagocytic activity of Kupffer cells, latex particles (0.8 μm in diameter, 0.05mg/100 g b.w.) were injected through the portal vein for one min before fixation. For preservation of lipid droplets in the cytoplasm, a series of these procedure were performed under ice cold temperature until the end of dehydration.

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Mast cell-nerve fiber interaction: Ultrastructural studies

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100159680 ◽

1990 ◽

Vol 48 (3) ◽

pp. 428-429

Author(s):

E.Y. Chi ◽

M.L. Su ◽

Y.T. Tien ◽

W.R. Henderson

Keyword(s):

Mast Cell ◽

Mast Cells ◽

Substance P ◽

Nerve Fiber ◽

Sensory Nerves ◽

Ultrastructural Studies ◽

Morphological Studies ◽

Granulocyte Infiltration ◽

Intracutaneous Injection ◽

Recent Attention

Recent attention has been directed to the interaction of the nerve and immune systems. The neuropeptide substance P, a tachykinnin which is a neurotransmitter in the central and peripheral nervous systems produces tissue swelling, augemntation of intersitial fibrin deposition and leukocyte infiltration after intracutaneous injection. There is a direct correlation reported between the extent of mast cell degranulation at the sites of injection and the tissue swelling or granulocyte infiltration. It has previously been demonstrated that antidromic electrical stimulation of sensory nerves induces degranulation of cutaneous mast cells, cutaneous vasodilation and augmented vascular permeability. Morphological studies have documented a close anatiomical association between mast cells and nonmyelinated nerves, that contain substance P and other neuropeptides. However, the presence of mast cells within nerve fasicles has not been previously examined ultrastructurally. In this study, we examined ultrastructurally the distribution of mast cells in the nerve fiber bundles located in the muscular connective tissue of rat tongues (n=20).

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Histochemical and Ultrastructural Studies of the Digestive Epithelium in a Gastropod Gametolytic Organ

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s143192760000252x ◽

1980 ◽

Vol 38 ◽

pp. 568-569

Author(s):

R. L. Reeder ◽

S. H. Rogers ◽

W. A. Shannon

Keyword(s):

Acid Phosphatase ◽

Genital Tract ◽

Reproductive Tract ◽

Ultrastructural Level ◽

Conclusive Evidence ◽

Digestive Organ ◽

Limited Information ◽

Ultrastructural Studies ◽

Morphological Studies

Numerous morphological studies have dealt with the spermatheca of pulmonate gastropods. This globular organ, which is attached to the female portion of the reproductive tract by a long duct in these monoecious animals, has had various functions ascribed to it. Recent histochemical demonstrations of deoxyribonuclease, ribonuclease, protease, and acid phosphatase have provided, however, conclusive evidence that it is a digestive organ for the degradation of superfluous sperm and genital tract secretions. Only limited information concerning the spermatheca is available at the ultrastructural level, a fact providing the stimulus for the present study of this organ in Sonorella santaritana, a desert mountain snail from Arizona.

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