scholarly journals Report on Incidence of Malvastrum coromandelianum to Vein Clearing Virus Disease (Malvastrum Yellow Vein Virus Disease) in Madurai

2020 ◽  
Vol 9 (11) ◽  
pp. 3743-3746
Author(s):  
K. Kalaichelvi
Keyword(s):  
Virus Disease ◽  
Yellow Vein ◽  
Vein Clearing ◽  
Malvastrum Coromandelianum

Development of a sensitive and reliable reverse transcription droplet digital PCR assay for the detection of citrus yellow vein clearing virus

2018 ◽  
Vol 164 (3) ◽  
pp. 691-697 ◽  
Author(s):  
Yingjie Liu ◽  
Yingli Wang ◽  
Qin Wang ◽  
Yanhui Zhang ◽  
Wanxia Shen ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Yellow Vein ◽  
Pcr Assay ◽  
Vein Clearing

The Titer of Citrus Yellow Vein Clearing Virus is Positively Associated with the Severity of Symptoms in Infected Citrus Seedlings

Plant Disease ◽  
2021 ◽  
Author(s):  
Yu Bin ◽  
Jianjian Xu ◽  
Zhimin Ma ◽  
Yu Duan ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Biological Properties ◽  
Yellow Vein ◽  
Citrus Limon ◽  
Cdna Clones ◽  
Symptom Development ◽  
Vein Clearing ◽  
Reverse Transcription Pcr ◽  
The Family ◽  
Citrus Production ◽  
Dialeurodes Citri

Citrus yellow vein clearing virus is a new member of the genus Mandarivirus in the family Alphaflexiviridae. Citrus yellow vein clearing virus (CYVCV) is the causal agent of citrus yellow vein clearing disease and is widely distributed in Pakistan, India, Turkey, and China. CYVCV is transmitted from citrus to citrus by Dialeurodes citri, grafting, and contaminated knife blades, threatening citrus production. In this study, four infectious full-length cDNA clones of CYVCV (namely AY112, AY132, AY212, and AY221) derived from CYVCV isolate AY were obtained through yeast homologous recombination and inoculated to ‘Eureka’ lemon (Citrus limon Burm. f.) by Agrobacterium-mediated vacuum infiltration. Pathogenicity analysis indicated that the clones AY212 and AY221 caused more severe symptoms than AY112 and AY132. Northern blot and quantitative reverse transcription PCR (qRT-PCR) analyses showed that the titers of virulent clones (AY212 and AY221) were significantly higher than those of attenuated clones (AY112 and AY132) in the infected ‘Eureka’ lemon (Citrus limon Burm. f.) seedlings. Subsequent comparative studies of viral infectivity, accumulation, and symptoms induced by AY221 in nine citrus cultivars indicated that (i) the infectivity of AY221 varied from 25% to 100% among different cultivars; (ii) ‘Oota’ ponkan (C. reticulata L.) showed the lowest infection rate with mild symptoms, which might be a useful resource for CYVCY-resistance genes; (iii) CYVCV titer was positively associated with the symptom development in infected citrus seedlings. In general, this report revealed the biological properties of CYVCV, thus laying a foundation for further investigation of pathogenic mechanisms in this virus.

Genetic analysis of Okra Yellow Vein Mosaic Virus disease resistance in wild relative of okra Abelmoschus angulosus Wall. ex Wight & Arn

2019 ◽  
Vol 17 (04) ◽  
pp. 346-351
Author(s):  
SamanthiKumari Wasala ◽  
Sumudu I. Senevirathne ◽  
Jayantha Bandara Senanayake ◽  
Anuradini Navoditha
Keyword(s):  
Disease Resistance ◽  
Mosaic Virus ◽  
Disease Severity ◽  
Virus Disease ◽  
Disease Incidence ◽  
Severity Index ◽  
Yellow Vein ◽  
Wild Relative ◽  
Yellow Vein Mosaic Virus ◽  
Progress Curve

AbstractWild relative of okra, Abelmoschus angulosus Wall. ex Wight & Arn. was identified as a resistant germplasm for Okra Yellow Vein Mosaic Virus (OYVMV) which is the devastating disease for okra cultivation in Sri Lanka. The mode of resistance of OYVMV in A. angulosus was studied with the aim of tagging responsible genes for the disease resistance. Wide hybridization was performed between A. angulosus and highly virus susceptible A. esculentus variety, MI-7. Very poor seed setting was observed in F1 and F2 generations due to post zygotic abortion. Disease screening was carried out using F1 and F2:3 populations along with parents in the field under induced disease pressure. Disease severity index and area under disease progress curve were calculated to measure disease severity. Number of genes segregating for OYVMV disease resistance was calculated for the F2:3 generation. Modified Wright's formula was used to estimate the effective gene number and mode of inheritance by a quantitative method. A χ2 test was performed for qualitative analysis. Plants of A. angulosus were totally free of virus incidence while 100% disease incidence was observed in the variety MI-7. F2:3 population showed between and within progeny segregation for disease incidence. Results indicated that the disease susceptibility was dominant over resistance. χ2 analysis revealed that the segregation of disease severity was significantly fit to the gene model of 9:6:1 (χ2 = 0.1757 at P ≤ 0.05) suggesting the disease resistance in A. angulosus is governing by two recessive genes in an additive manner. Result was confirmed by the quantitative analysis.

scholarly journals First Report of Beet necrotic yellow vein virus Infecting Spinach in California

Plant Disease ◽  
2010 ◽  
Vol 94 (5) ◽  
pp. 640-640 ◽  
Author(s):  
H.-Y. Liu ◽  
B. Mou ◽  
K. Richardson ◽  
S. T. Koike
Keyword(s):  
Spinacia Oleracea ◽  
Sandwich Elisa ◽  
Chenopodium Quinoa ◽  
Yellow Vein ◽  
Mechanical Transmission ◽  
Polymyxa Betae ◽  
Rt Pcr ◽  
First Report ◽  
Vein Clearing ◽  
Rigid Rod

In 2009, plants from two spinach (Spinacia oleracea) experimental fields in Monterey County and one commercial spinach field in Ventura County of California exhibited vein-clearing, mottling, interveinal yellowing, and stunting symptoms. For experimental fields, up to 44% of spinach plants have symptoms. With a transmission electron microscope, rigid rod-shaped particles with central canals were observed from plant sap of the symptomatic spinach. Analysis with a double-antibody sandwich-ELISA assay for Beet necrotic yellow vein virus (BNYVV) showed that all 10 symptomatic plants we tested were positive and 5 asymptomatic plants were negative. Symptomatic spinach from both counties was used for mechanical transmission experiments. Chenopodium quinoa, Tetragonia expansa, and Beta vulgaris (sugar beet) showed chlorotic local lesions and B. macrocarpa and spinach showed vein-clearing, mottling, and systemic infections. To further confirm the presence of BNYVV, reverse transcription (RT)-PCR was conducted. Total RNA was extracted from field- and mechanically inoculated symptomatic spinach plants using an RNeasy Plant Kit (Qiagen Inc., Valencia, CA) and used as a template in RT-PCR. Forward and reverse primers specific to the BNYVV RNA-3 P25 protein gene from the beet isolate were used (2). Amplicons of the expected size (approximately 860 bp) were obtained. Four RT-PCR products were sequenced and the sequences were identical (GenBank Accession No. GU135626). Sequences from the spinach plants had 97 to 99% nucleotide and 94 to 100% amino acid identity with BNYVV RNA-3 P25 protein sequences available in the GenBank. On the basis of the data from electron microscopy, indicator plants, serology, and cDNA sequencing, the virus was identified as BNYVV. BNYVV has been reported from spinach fields in Italy (1). To our knowledge, this is the first report of BNYVV occurring naturally on spinach in California. Since BNYVV is transmitted by the zoospores of the soil-inhabiting plasmodiophorid Polymyxa betae, it could be a new threat to spinach production in the state. References: (1) C. R. Autonell et al. Inf. Fitopatol. 45:43, 1995. (2) H.-Y. Liu and R. T. Lewellen, Plant Dis. 91:847, 2007.

scholarly journals Identification of Dialeurodes citri as a Vector of Citrus yellow vein clearing virus in China

Plant Disease ◽  
2019 ◽  
Vol 103 (1) ◽  
pp. 65-68 ◽  
Author(s):  
Y. H. Zhang ◽  
C. H. Liu ◽  
Q. Wang ◽  
Y. L. Wang ◽  
C. Y. Zhou ◽  
...  
Keyword(s):  
Viral Disease ◽  
Yellow Vein ◽  
Sour Orange ◽  
Rt Pcr ◽  
Panonychus Citri ◽  
Vein Clearing ◽  
Access Period ◽  
Inoculation Access Period ◽  
Dialeurodes Citri ◽  
The Mean

In 2009, a new citrus viral disease caused by Citrus yellow vein clearing virus (CYVCV) was first discovered in China and now CYVCV is widely distributed in the field. CYVCV is transmissible by grafting and is spread by aphids from lemon to bean, and from bean to bean. However, until now, no vector has been shown to transmit CYVCV from citrus to citrus. In this study, after a 24-h acquisition access period (AAP), CYVCV was tested for in Dialeurodes citri (Ashmead), Panonychus citri McGregor, and Aphis citricidus (Kirkaldy) by quantitative RT-PCR. After an AAP of 48 h, groups of adults of D. citri, P. citri, and A. citricidus were given a 48 h inoculation access period on cultivar Daidai sour orange seedlings. Three, 6, and 12 months post-transmission by D. citri, CYVCV was detected in the receptor plants, and the mean incidence of infected trees was 31.9, 39.1, and 39.1%, respectively. CYVCV was not transmitted to citrus by P. citri or A. citricidus. This is the first report of the ability of D. citri to transmit CYVCV from infected to healthy citrus under laboratory conditions.

scholarly journals Molecular Variation of Satellite DNAβ Molecules Associated with Malvastrum yellow vein virus and Their Role in Pathogenicity

2008 ◽  
Vol 74 (6) ◽  
pp. 1909-1913 ◽  
Author(s):  
Wei Guo ◽  
Tong Jiang ◽  
Xian Zhang ◽  
Guixin Li ◽  
Xueping Zhou
Keyword(s):  
Petunia Hybrida ◽  
Yunnan Province ◽  
Geographical Origin ◽  
Geographical Location ◽  
Yellow Vein ◽  
Molecular Variation ◽  
Infectious Clones ◽  
Malvastrum Coromandelianum ◽  
Geographical Locations ◽  
Limited Sequence

ABSTRACT Previous studies have found that the diversity of begomovirus-associated DNAβ satellites is related to host and geographical origin. In this study, we have cloned and sequenced 20 different isolates of DNAβ molecules associated with Malvastrum yellow vein virus (MYVV) isolated from Malvastrum coromandelianum plants in different geographical locations of Yunnan Province, China. Analyses of their molecular variation indicate that the satellites are clustered together according to their geographical location but that they have only limited sequence diversity. Infectivity tests using infectious clones of MYVV and its associated DNAβ molecule indicate that MYVV DNAβ is indispensable for symptom induction in Nicotiana benthamiana, N. glutinosa, Petunia hybrida, and M. coromandelianum plants. Furthermore, we showed that MYVV interacts functionally with heterologous DNAβ molecules in N. benthamiana plants.

scholarly journals EVALUATION OF ORGANIC AMENDMENTS AND INSECTICIDES AGAINST OKRA YELLOW VEIN MOSAIC VIRUS AND ITS VECTOR

2020 ◽  
Vol 36 (1) ◽  
pp. 13-20
Author(s):  
A. Munir ◽  
S. Ali ◽  
M. A. Zeshan ◽  
M. U. Ghani ◽  
A. A. Khan
Keyword(s):  
Organic Matter ◽  
Mosaic Virus ◽  
Disease Severity ◽  
Virus Disease ◽  
Block Design ◽  
Poultry Manure ◽  
Yellow Vein ◽  
Effective Control ◽  
Management Approach ◽  
Yellow Vein Mosaic Virus

Okra (Abelmoschus esculentus L.) is susceptible to number of diseases like Yellow Vein Mosaic Virus disease, Damping Off, Fusarium wilt, Powdery Mildew and Enation Leaf Curl. The Okra is favorite host of whitefly which transmits the okra Yellow Vein Mosaic Virus (OYVMV). OYVMV disease causes considerable yield losses in Okra. Experiment was conducted to check the efficacy of organic matter (poultry manure, leaves) and insecticides (Acetamiprid, Diafenthiuron, Pyridine Carboxamide) against OYVMV and its whitefly vector. In this experiment, ten okra varieties were sown in randomized complete block design (RCBD). Organic matter and insecticides were applied at recommended doses (@5ml/L) on weekly intervals. Among insecticides, Acetamiprid showed the best result to manage whitefly infestation with 60.01% mortality and OYVMV disease severity with 53.98% efficiency. Polo (Diafenthiuron) also showed good results to manage whitefly (56.36%) and OYVMVD (46.34%), but Ulala (Pyridine Carboxamide) insecticide was least effective to control whitefly population (47.27%) and to manage the infection of OYVMV disease (43.91%). Leaf manure was more effective with 61.16% efficiency as compared to poultry manure (56.01%) against OYVMV disease severity. New chemistry insecticide (acetamiprid) gave the most effective control of whitefly and transmission of OYVMV and could be used in place of conventional insecticides. Leaf manure proved significant defense activator in okra plants with effective control of disease and could be used as eco-friendly management approach.

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