The Titer of Citrus Yellow Vein Clearing Virus is Positively Associated with the Severity of Symptoms in Infected Citrus Seedlings

Plant Disease ◽  
2021 ◽  
Author(s):  
Yu Bin ◽  
Jianjian Xu ◽  
Zhimin Ma ◽  
Yu Duan ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Biological Properties ◽  
Yellow Vein ◽  
Citrus Limon ◽  
Cdna Clones ◽  
Symptom Development ◽  
Vein Clearing ◽  
Reverse Transcription Pcr ◽  
The Family ◽  
Citrus Production ◽  
Dialeurodes Citri

Citrus yellow vein clearing virus is a new member of the genus Mandarivirus in the family Alphaflexiviridae. Citrus yellow vein clearing virus (CYVCV) is the causal agent of citrus yellow vein clearing disease and is widely distributed in Pakistan, India, Turkey, and China. CYVCV is transmitted from citrus to citrus by Dialeurodes citri, grafting, and contaminated knife blades, threatening citrus production. In this study, four infectious full-length cDNA clones of CYVCV (namely AY112, AY132, AY212, and AY221) derived from CYVCV isolate AY were obtained through yeast homologous recombination and inoculated to ‘Eureka’ lemon (Citrus limon Burm. f.) by Agrobacterium-mediated vacuum infiltration. Pathogenicity analysis indicated that the clones AY212 and AY221 caused more severe symptoms than AY112 and AY132. Northern blot and quantitative reverse transcription PCR (qRT-PCR) analyses showed that the titers of virulent clones (AY212 and AY221) were significantly higher than those of attenuated clones (AY112 and AY132) in the infected ‘Eureka’ lemon (Citrus limon Burm. f.) seedlings. Subsequent comparative studies of viral infectivity, accumulation, and symptoms induced by AY221 in nine citrus cultivars indicated that (i) the infectivity of AY221 varied from 25% to 100% among different cultivars; (ii) ‘Oota’ ponkan (C. reticulata L.) showed the lowest infection rate with mild symptoms, which might be a useful resource for CYVCY-resistance genes; (iii) CYVCV titer was positively associated with the symptom development in infected citrus seedlings. In general, this report revealed the biological properties of CYVCV, thus laying a foundation for further investigation of pathogenic mechanisms in this virus.

scholarly journals Development of Infectious cDNA Clones of Citrus Yellow Vein Clearing Virus Using a Novel and Rapid Strategy

2018 ◽  
Vol 108 (10) ◽  
pp. 1212-1218 ◽  
Author(s):  
Tiantian Cui ◽  
Yu Bin ◽  
Jianhong Yan ◽  
Pengying Mei ◽  
Zhongan Li ◽  
...  
Keyword(s):  
Causal Agent ◽  
Full Length ◽  
Yellow Vein ◽  
Wild Type Virus ◽  
Economic Losses ◽  
Citrus Limon ◽  
Cdna Clones ◽  
Rt Pcr ◽  
Long Distance ◽  
Vein Clearing

Yellow vein clearing disease (YVCD) causes significant economic losses in lemon and other species of citrus. Usually, citrus yellow vein clearing virus (CYVCV) is considered to be the causal agent of YVCD. However, mixed infection of CYVCV and Indian citrus ringspot virus (ICRSV) or other pathogens is often detected in citrus plants with YVCD. In this study, we re-examined the causal agent of YVCD to fulfill Koch’s postulates. First, the full-length genome of CYVCV isolate AY (CYVCV-AY) was amplified by long-distance RT-PCR from a Eureka lemon (Citrus limon) tree with typical YVCD symptoms. The genomic cDNAs were then cloned into a ternary Yeast-Escherichia coli-Agrobacterium tumefaciens shuttle vector, pCY, using transformation-associated recombination (TAR) strategy, and 15 full-length cDNA clones of CYVCV-AY were obtained. Subsequently, four of these clones were selected randomly and inoculated on Jincheng (C. sinensis) seedlings through Agrobacterium-mediated vacuum-infiltration, and it was found that 80 to 100% of inoculated plants were infected with CYVCV by RT-PCR at 20 to 40 days postinoculation (dpi) and by direct tissue blot immunoassay at 60 dpi. The progeny of CYVCV-AY from cDNA clones caused typical symptoms of YVCD such as yellow vein clearing, leaf distortion, and chlorosis, which were the same as that elicited by wild-type virus. Finally, the regeneration of CYVCV-AY genome was confirmed by long-distance RT-PCR in lemon trees inoculated with the infectious cDNA clone. These results proved that CYVCV was the primary causal agent of YVCD. This is the first report on the development of infectious cDNA clones of CYVCV, which lays the foundation for further studies on viral gene functions and virus−host interactions.

scholarly journals Identification of Dialeurodes citri as a Vector of Citrus yellow vein clearing virus in China

Plant Disease ◽  
2019 ◽  
Vol 103 (1) ◽  
pp. 65-68 ◽  
Author(s):  
Y. H. Zhang ◽  
C. H. Liu ◽  
Q. Wang ◽  
Y. L. Wang ◽  
C. Y. Zhou ◽  
...  
Keyword(s):  
Viral Disease ◽  
Yellow Vein ◽  
Sour Orange ◽  
Rt Pcr ◽  
Panonychus Citri ◽  
Vein Clearing ◽  
Access Period ◽  
Inoculation Access Period ◽  
Dialeurodes Citri ◽  
The Mean

In 2009, a new citrus viral disease caused by Citrus yellow vein clearing virus (CYVCV) was first discovered in China and now CYVCV is widely distributed in the field. CYVCV is transmissible by grafting and is spread by aphids from lemon to bean, and from bean to bean. However, until now, no vector has been shown to transmit CYVCV from citrus to citrus. In this study, after a 24-h acquisition access period (AAP), CYVCV was tested for in Dialeurodes citri (Ashmead), Panonychus citri McGregor, and Aphis citricidus (Kirkaldy) by quantitative RT-PCR. After an AAP of 48 h, groups of adults of D. citri, P. citri, and A. citricidus were given a 48 h inoculation access period on cultivar Daidai sour orange seedlings. Three, 6, and 12 months post-transmission by D. citri, CYVCV was detected in the receptor plants, and the mean incidence of infected trees was 31.9, 39.1, and 39.1%, respectively. CYVCV was not transmitted to citrus by P. citri or A. citricidus. This is the first report of the ability of D. citri to transmit CYVCV from infected to healthy citrus under laboratory conditions.

scholarly journals Biological properties of Beet soil-borne mosaic virus and Beet necrotic yellow vein virus cDNA clones produced by isothermal in vitro recombination: Insights for reassortant appearance

Virology ◽  
2018 ◽  
Vol 518 ◽  
pp. 25-33 ◽  
Author(s):  
Marlene Laufer ◽  
Hamza Mohammad ◽  
Edgar Maiss ◽  
Katja Richert-Pöggeler ◽  
Mattia Dall'Ara ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Biological Properties ◽  
Yellow Vein ◽  
Cdna Clones ◽  
In Vitro Recombination ◽  
Virus Cdna

scholarly journals First Report of Citrus yellow vein clearing virus on Lemon in Yunnan, China

Plant Disease ◽  
2014 ◽  
Vol 98 (12) ◽  
pp. 1747-1747 ◽  
Author(s):  
H. M. Chen ◽  
Z. A. Li ◽  
X. F. Wang ◽  
Y. Zhou ◽  
K. Z. Tang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Yellow Vein ◽  
Yield Reduction ◽  
Mechanical Transmission ◽  
Citrus Limon ◽  
Herbaceous Plant ◽  
Sour Orange ◽  
Leaf Rolling ◽  
Mexican Lime ◽  
Vein Clearing

Eureka lemon (Citrus limon) is an economically important crop in China. In 2009, a new disease was observed on Eureka lemon in an orchard of Ruili city in Yunnan Province. Nearly 10% of the plants were symptomatic with yellow vein clearing on lateral veins, combined with leaf crinkling and warping of young leaves. Water-soaked appearances were also seen on the adaxial leaf veins. The symptoms were present on mature leaves, resulting in almost 20% yield reduction. The symptoms on leaves were more severe in spring and autumn than in summer. This disease was transmitted by grafting from infected Eureka lemon to six lemon (C. limon) varieties (Femminello, Fino, Villafranca, Verna, Kutdiken, and Monachello) and two sour orange (C. aurantium) varieties (Daidai, Morocco), with inoculated plants showing similar symptoms. Variable symptoms were observed on other hosts, including severe yellowing and vein clearing on Dweet tangor (C. reticulata × C. sinensis), slight vein clearing on Tongshui 72-1 sweet orange (C. sinensis) and Marsh grapefruit (C. paradisi), but no symptoms were observed on Etrog citron (C. medica), Mexican lime (C. aurantifolia), or Ponkan (C. reticulata). When infected branches from Etrog citron, Mexican lime, and Ponkan were grafted onto healthy Morocco sour orange, typical yellowing and vein clearing symptoms were observed. In the mechanical transmission trials using eight herbaceous plant species, leaf rolling, shrinking, and pointed necrosis were observed on Vigna unguiculata, slight leaf rolling symptoms were observed on Capsicum frutescens, whereas Lycopersicum esculenum, Nicotiana benthamiana, Momordica charantia, Solanum melongena, Cucumis sativus, and Sesamum indicum remained symptomless. Symptom expression was influenced by temperature, being visible between 18 and 24°C but disappearing over 32°C. Crude extracts from young symptomatic lemon leaves were negatively stained for transmission electron microscopy observation (1) and showed filamentous virus particles of 13 to 15 × 400 to 1,000 nm. The described combination of symptoms in citrus species and herbaceous indicators, and virus morphology revealed by electron microscopy, suggested presence of Citrus yellow vein clearing virus (CYVCV). RNA extractions were made from 52 samples of Eureka lemon with yellow vein clearing symptoms. A one-step RT-PCR using primer pair (sense: 5′-TACCGCAGCTATCCATTTCC-3′ and antisense: 5′-GCAGAAATCCCGAACCACTA-3′), designed from coat protein gene of CYVCV genome (GenBank Accession No. JX040635) (1), was performed. All of the symptomatic samples yielded the expected cDNA fragment and no amplicon was obtained from healthy plants. Three PCR amplicons were selected for cloning and sequencing. BLAST analysis showed that these nucleotide sequences (KJ859679 to 81) had greater than 97% nucleotide identity with the corresponding region of CYVCV reference genome (2), and therefore, this disease was associated with CYVCV. Symptoms resembling CYVCV infection were observed in China before (3); however, to our knowledge, this is the first proven report of CYVCV present in China. The identification of the pathogen now allows for appropriate field management and control measures. References: (1) V. Grimaldi et al. 13th IOCV Conference, 343-345, 1996. (2) G. Loconsole et al. Phytopathology 102:1168, 2012. (3) C. Zhou et al. 18th IOCV Conference, 44, 2010.

PDO Rotonda’s Red Eggplant Extract: In vitro Determination of Biological Properties and Minerals Bioaccessibility

2020 ◽  
Vol 16 (1) ◽  
pp. 65-74
Author(s):  
Ortensia Ilaria Parisi ◽  
Mariarosa Ruffo ◽  
Fabio Amone ◽  
Rocco Malivindi ◽  
Domenico Gorgoglione ◽  
...  
Keyword(s):  
Control Sample ◽  
Specific Area ◽  
Biological Properties ◽  
Ammonium Molybdate ◽  
Antihypertensive Activity ◽  
Protected Designation Of Origin ◽  
The Family ◽  
Oil Red O Staining

Background: The Rotonda’s Red Eggplant belongs to the family of Solanum aethiopicum and it is cultivated in a specific area of Potenza (Basilicata, South of Italy) including villages of Rotonda, Viggianello, Castelluccio Superiore and Castelluccio Inferiore. The Red Eggplant cultivated in this area has gained the PDO, “Protected Designation of Origin”. Objective: The aim of this research was to evaluate the use of PDO Rotonda’s Red Eggplant extract as a possible nutraceutical supplement. The antioxidant, antihypertensive, hypoglycemic, and hypolipidemic properties were in vitro evaluated. Methods: The antioxidant activity was investigated by evaluating the scavenging properties against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals and by performing the Ammonium Molybdate and Folin-Ciocalteu assay. The hypoglycemic and antihypertensive activity was studied by evaluating the α-Amylase, α-Glucosidase and Angiotensin Converting Enzyme, respectively, inhibiting activity. In order to evaluate the hypolipidemic activity, the pancreatic lipase inhibiting property was determined and Oil Red O staining assay was performed. Finally, to evaluate the possible use of this extract as a minerals supplement, Selenium, Potassium and Chrome bioaccessibility was studied. Results: The obtained results underline the good antioxidant, hypoglycemic, antihypertensive and hypolipidemic in vitro properties of the PDO Rotonda’s Red Eggplant extract. Moreover, the obtained data show a higher minerals bioaccessibility and this higher value could be ascribable to the natural phytocomplex of PDO Rotonda’s Red Eggplant, which increases the minerals bioaccessibility if compare it with a control sample. Conclusion: The obtained results show that PDO Rotonda’s Red Eggplant extract, might be used as a possible nutraceutical supplement, along with traditional therapies, both for its biological properties and for its minerals bioaccessibility value.

scholarly journals Traditional, phytochemical, and biological aspects of Rosa alba L.: a systematic review

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Anju Verma ◽  
Rajni Srivastava ◽  
Pankaj Kumar Sonar ◽  
Ramprakash Yadav
Keyword(s):  
Systematic Review ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Research Work ◽  
Biological Properties ◽  
Medicinal And Aromatic Plants ◽  
Research Papers ◽  
Northwest Africa ◽  
The Family ◽  
White Rose

Abstract Background Rosa alba L. belongs to the family Rosaceae. This species is widely cultivated in Europe, Asia, North America, and Northwest Africa due to its fragrance, ornamental, and medicinal values. It is commonly known as white oil-bearing rose, white rose, white rose of York, backyard rose, and sufaid gulab. Main text Rosa alba L. has many biological properties like antioxidant, antimicrobial, antifungal, antifertility, teratogenic, memory enhancing, cytotoxic, and genotoxic activities. The essential oil of Rosa alba L. possesses good antimicrobial activity and consists of many chemical constituents like- citronellol, geraniol, nerol, linalool, citral, carvacrol, eugenol, etc. Conclusion This article briefly reviews the cultivation, traditional uses, phytochemistry, and biological activities of Rosa alba L. Many research papers have been published on the proposed plant and still, there is a very vast scope of research on it. Therefore, this review will be very fruitful for those scientists who are doing or plan to do research work on this plant. All the scientific findings written in this review are explored from Google web, Google Scholar, PubMed, ScienceDirect, Medicinal and Aromatic Plants Abstracts (MAPA), and SciFinder. To date, it is the first systematic review article of such kind, on this plant.

Development of a sensitive and reliable reverse transcription droplet digital PCR assay for the detection of citrus yellow vein clearing virus

2018 ◽  
Vol 164 (3) ◽  
pp. 691-697 ◽  
Author(s):  
Yingjie Liu ◽  
Yingli Wang ◽  
Qin Wang ◽  
Yanhui Zhang ◽  
Wanxia Shen ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Yellow Vein ◽  
Pcr Assay ◽  
Vein Clearing

scholarly journals First Report from Syria of Citrus tristeza virus in Citrus spp.

Plant Disease ◽  
2008 ◽  
Vol 92 (10) ◽  
pp. 1468-1468
Author(s):  
R. Abou Kubaa ◽  
K. Djelouah ◽  
A. M. D'Onghia ◽  
R. Addante ◽  
M. Jamal
Keyword(s):  
Citrus Tristeza Virus ◽  
Sweet Orange ◽  
Citrus Limon ◽  
Potential Threat ◽  
First Report ◽  
Mexican Lime ◽  
Vein Clearing ◽  
Citrus Varieties ◽  
Tristeza Virus ◽  
Citrus Tristeza

During the spring of 2006, the main Syrian citrus-growing areas of Lattakia (Jableh, Aledyye, Eseelya, Siano, and Hresoon provinces) and Tartous (Almintar, Aljammase, Karto, Majdaloonelbahr, Yahmour, Amreet, Althawra, and Safita provinces) were surveyed to assess the presence of Citrus tristeza virus (CTV). Eight nurseries (approximately 130 plants per nursery), two budwood source fields (approximately 230 trees per field), and 19 groves (approximately 60 trees per grove) containing the main citrus varieties were visually inspected and sampled for serological assays. The hierarchical sampling method was carried out in each selected grove (2). Infected samples were collected from two nurseries, two budwood source fields, and six groves. Stems and leaf petioles from nursery trees and flower explants from the groves were collected and analyzed for CTV by direct tissue blot immunoassay (DTBIA) with the commercial kit from Plantprint (Valencia, Spain). Of 2,653 samples tested, 89 (4%) CTV-infected plants were detected. Five citrus varieties were found to be infected and Meyer lemon (Citrus limon ‘Meyer’) had the highest incidence at 16%. Numerous sweet orange varieties (Citrus sinensis L.) were found to be highly infected in the field, but only the Washington navel sweet orange was found to be infected in the nurseries. No clear CTV symptoms were observed during the survey. Samples that were positive for CTV by DTBIA were also positive by biological indexing on Mexican lime (C. aurantifolia) and immunocapture-reverse transcription-PCR as described by Nolasco et al. (3). Coat protein gene sequences obtained from five selected clones of a Syrian CTV isolate (GenBank Accession No. EU626555) showed more than 99 and 98% nucleotide sequence identity to a Jordanian CTV isolate (GenBank Accession No. AY550252) and the VT isolate (GenBank Accession No. U56902), respectively. Almost all infected samples induced moderate vein clearing symptoms when grafted to Mexican lime. Symptoms of vein clearing, leaf cupping, stunting, and stem pitting on Mexican lime were induced by graft transmission of CTV from one Valencia sample from the Tartous area. The viral inoculum is widely and randomly distributed in commercial groves, especially in the southern Tartous area and in some nurseries. To our knowledge, this is the first report of CTV in Syria. However, CTV was reported from the neighboring citrus-growing countries of Lebanon, Turkey, and Jordan (1), and the severe seedling yellows strain is present in this area, which poses a potential threat to Syrian citriculture. References: (1) G. H. Anfoka et al. Phytopathol. Mediterr. 44:17, 2005. (2) G. Hughes and T. R. Gottwald, Phytopathology 88:715, 1998. (3) G. Nolasco et al. Eur. J. Plant Pathol. 108:293, 2002.

Expression of recombinant platelet-derived growth factor A- and B-chain homodimers in rat-1 cells and human fibroblasts reveals differences in protein processing and autocrine effects

1988 ◽  
Vol 8 (7) ◽  
pp. 2753-2762
Author(s):  
M Bywater ◽  
F Rorsman ◽  
E Bongcam-Rudloff ◽  
G Mark ◽  
A Hammacher ◽  
...  
Keyword(s):  
Growth Factor ◽  
Human Fibroblasts ◽  
Soft Agar ◽  
Biological Properties ◽  
Platelet Derived Growth Factor ◽  
Cdna Clones ◽  
Metabolic Labeling ◽  
High Saturation ◽  
Dimeric Protein ◽  
A Chain

The autocrine effects of platelet-derived growth factor (PDGF) A- and B-chain homodimers (PDGF-AA and PDGF-BB) on rat-1 cells and human fibroblasts have been investigated by using human PDGF A- and B-chain cDNA clones expressed in a retroviral vector. Infection with replication-defective virus carrying the B-chain cDNA resulted in a phenotypical transformation resembling that induced by simian sarcoma virus. The resulting cells were focus forming in monolayer cultures, grew to high saturation densities, and formed large colonies in soft agar. The PDGF A-chain transfectants showed no transformed morphology and lacked focus-forming activity but grew to high saturation density in monolayer culture and formed small colonies in soft agar. A similar but weaker effect was obtained with an A-chain cDNA variant containing a 69-base-pair insertion in the 3' end of the protein-coding domain. A- and B-chain transfectants released PDGF receptor-competing activity into the medium, but only the medium conditioned by the B-chain transfectants possessed potent mitogenic activity on human fibroblasts. Both types of transfectants had downregulated levels of PDGF receptors; however, the B-chain transfectants were downregulated to significantly lower levels. Metabolic labeling and immunoprecipitations with PDGF antiserum showed that the PDGF B-chain protein was processed to a 24-kilodalton cell-associated and a 30-kilodalton secreted dimeric protein. The A-chain protein was rapidly secreted as a 31-kilodalton dimeric protein. The present study shows a marked difference in the autocrine effects of PDGF-AA and -BB expressed under the control of a retroviral promoter and suggests that different biological properties may be assigned to these two PDGF isoforms.

scholarly journals Biological Properties of a Citral-Enriched Fraction of Citrus limon Essential Oil

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1290
Author(s):  
Marzia Pucci ◽  
Stefania Raimondo ◽  
Chiara Zichittella ◽  
Vincenza Tinnirello ◽  
Valeria Corleone ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Essential Oil ◽  
Biological Activities ◽  
Protective Action ◽  
Biological Properties ◽  
Citrus Limon ◽  
Tnf Α ◽  
Pre Treatment ◽  
Flavoring Agent ◽  
And Oxidative Stress

Lemon essential oil (LEO) is a well-known flavoring agent with versatile biological activities. In the present study, we have isolated and characterized four citral-enriched fractions of winter LEO. We reported that in murine and human macrophages the pre-treatment with a mix of these fractions (Cfr-LEO) reduces the expression of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 induced by LPS. In addition, Cfr-LEO counteracts LPS-induced oxidative stress, as shown by the increase in the GSH/GSSG ratio in comparison to cells treated with LPS alone. Overall, the results reported here encourage the application of EO fractions, enriched in citral, in the nutraceutical industry, not only for its organoleptic properties but also for its protective action against inflammation and oxidative stress.

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