EXTRACTION METHODS AND ADSORPTION KINETICS OF POTASSIUM IN CALCAREOUS SOILS OF MULTAN

Understanding the dynamics of potassium (K) and its availability is fundamental in its efficient use as an important nutrient. The objectives of present study were to compare standard K extraction methods for extractable K and kinetics of K adsorption on five different textured soils. The soils were analyzed for physico-chemical properties, extractable K contents and kinetics of K adsorption. The soils had variable contents of soluble (8-52mg kg-1), exchangeable (13-215mg kg-1), fixed (488-1836mg kg-1), structural (2936-26362mg kg-1) and total K (3438-28517mg kg-1). Except soluble K, the amount of K in different forms was significantly (P?0.05) correlated with clay contents, electrical conductivity (EC), cation exchange capacity (CEC) and calcium carbonate in soils. Different methods variated the extracted K in different textured soils. Mehlich-III, ammonium acetate, AB-DTPA, and calcium chloride extracted K was maximum in sandy clay loam (298mg kg-1, 267mg kg?1, 226mg kg-1, and 113mg kg-1) and lowest was in loamy sand (33mg kg-1, 24mg kg-1, 30mg kg-1, and 24mg kg-1) respectively. Elovich and power function models best explained the K kinetics adsorption, as rate of adsorption constants were negatively correlated with sand contents in the soil type. Pearson correlation showed that the extractability of K by different methods and kinetics of K adsorption were dependent on soil texture EC, CEC and calcium carbonate contents. Hence, soil physico-chemical properties should be considered in recommending rate of K fertilization along with crop K demand and soil extractable K.

IMPACT OF DIFFERENT LEVELS OF BULK DENSITIES COMBINATION ON YIELD AND YIELD COMPONENT OF WHEAT (TRITICUM AESTIVUM L.)

An experiment was conducted on the impact of different levels of bulk densities combination on yield and yield components of wheat. Three bulk density levels 1.00-1.30 (A) g cm-3, 1.30-1.60 (B) g cm-3 and 1.60-1.90 (C) g cm-3 with three different depths (0-15, 16-30 and 31-45 cm) were used in the experiment using silt loam soil (Pedocals). The total treatments were 27 in the experiment with three replications. The experimental design was completely randomized design (CRD). The treatment means were checked at the confidence level of 95% of probability. The soil 0-45 cm deep was shifted from the field (no-till) to the net house and was act as control. The results showed that the number of tillers plant-1, number of leaves plant-1 and thousand grain yield were non-significantly affected by all the treatments while number of seed emerged, root length, dry root weight, plant height, grain yield , biological yield and harvest index were significantly influenced by various treatments. The highest number of seed germinated (8.00) was found in the treatment BBB (1.30-1.60 g cm-3 in 0-15 cm, 15-30 cm, 30-45 cm soil depth), BAB (1.30-1.60 g cm-3 in 0-15 cm, 1.00-1.30 g cm-3 in 15-30 cm and 1.30-1.60 g cm-3 in 30-45 cm soil depth) and BCB (1.30-1.60 g cm-3 in 0-15 cm, 1.60-1.90 g cm-3 in 15-30 cm and 1.30-1.60 g cm-3 in 30-45 cm) while the lowest number of seed emerged (3.6) was found in AAA (1.00-1.30 g cm-3 in 0-15 cm, 15-30 cm and 30-45 cm soil depth), ACA (1.00-1.30 g cm-3 in 0-15 cm, 1.60-1.90 g cm-3 in 15-30 cm and 1.00-1.30 g cm-3 in 30-45 cm soil depth) and CAC (1.60-1.90 g cm -3 in 0-15 cm, 1.00-1.30 g cm-3 in 15-30 cm and 1.60-1.90 g cm-3 in 30-45 cm soil depth). The highest root length (13.12 cm) was found in the treatment AAA while the lowest root length (10.05 cm) was found in CAC. The highest dry root weight (5.67g) was found in the treatment ABA while the lowest dry root weight (4.79g) was found in control treatment. The highest plant height (42.67 cm) was found in the treatment of BBA while the lowest plant height (29.67 cm) was found in control. The highest biological yield (12.1 metric ton ha-1) was found in the treatment ABA while the lowest biological yield (6.8 metric ton ha-1) was found in control. The highest grain yield (4.4 metric ton ha-1) was found by ABB while the lowest (2.7 metric ton ha-1) was found in the control. The highest harvest index (39.79%) was found in the control while the lowest harvest index (34.54%) was found in CCA. The experiment showed that increasing bulk density above 1.60 g cm-3 resulted in decreasing the yield and yield component of the winter wheat. The number of seed emerged, root length, dry root weight, plant height, grain yield, biological yield and harvest index of wheat were significantly affected by soil bulk density in various depths.

AVIAN ESCHERICHIA COLI INFECTION IN MEAT-TYPE CHICKEN: PREVALENCE AND PATHOLOGY

Avian colibacillosis is considered the major bacterial disease in the poultry industry worldwide. This study was conducted to determine prevalence and pathology by avian pathogenic Escherichia coli (APEC) in broiler chicken. Various organs of affected broilers were checked for the E. coli infection. One hundred and forty commercial poultry farms were divided into seven age groups (A, B, C, D, E, F, and G). Group A (1st week), B (2nd week), C (3rd week), D (4th week), E (5th week), F (6th week), G (7th to 9th week). Investigations were conducted from day 1 until the marketing of birds based on clinical findings. A total of 2491 (23.71% of sick birds) dead birds were observed positive for E. coli infection. Three forms of infection were observed i.e. omphalitis, colisepticaemia, and colibacillosis at the rate of 1.55, 17 and 56.65%, respectively. Omphalitis was confirmed by microbial culture and revealed in only groups A and B with 76.24 and 23.76 respectively. While, Colisepticaemia was observed at the rate of 3.11, 6.74, 20.18, 29.63, 19.16, and 22.18 in groups B, C, D, E, F, and G respectively. Whereas Colibacillosis was noticed as 0.075, 3.35, 5.99, 19.20, 26.70, 16.11 and 28.55% in groups A, B, C, D, E, F, and G respectively. Petechiation of heart, kidney, and gizzard besides hemorrhagic enteritis, hemorrhagic tracheitis, and necrotic foci on the liver and caseous exudates in air sacs were the prime gross lesions noticed in colibacillosis. Microscopically, no lesion was observed in serosa, muscularis externa, and submucosa. However, loss of epithelial tissue and breaches in the mucosal layer of the small intestine were observed. Furthermore, characteristic pink and grayish colonies were observed in E. coli cultures on McConkey’s and Blood agar respectively.

ASSESSMENT OF PHENOLIC COMPOUNDS, ANTIMICROBIAL ACTIVITY AND FREE RADICAL SCAVENGING POTENCY OF THREE SELECTED VEGETABLES

Phytochemicals are compounds derived from plants that are assumed to have defensive role against certain disease. They have antioxidants, anticancer, antimicrobial, antifungal, antiviral, antithrombic and anti-inflammatory properties. They have a high specificity to boost the immune system and play important role in the metabolism of hormones. The current study is based on qualitative and quantitative evaluation of total phenolics contents, phenolic compounds, antioxidant potential, free and bound phenolic acids in selected vegetables available at the local market of Hyderabad, Sindh, Pakistan. Two different extraction procedures ultrasonic-assisted base hydrolysis extraction and sonication extraction were used. Total 13 phenolic compounds were found and quantified by high-performance liquid chromatography (HPLC), in which Ferulic acid was quantified in a higher amount of 16.71 mg/g in bitter gourd. Total phenolic contents were determined by using Perklin-Elmer lambda UV/Visible spectrophotometer and higher concentration was found in Bitter gourd 92.56 mg 100/g as compared to Luffa and Brinjal with 79.03 and 66.56 mg 100/g, respectively. The antioxidant activity (DPPH assay) was measured at ?max of 517 nm, results revealed that Bitter gourd possessed the higher antioxidant activity with 182.61 µMol/g followed by Luffa and Brinjal with 112.94 and 82.96 µMol/g. The total Flavonoid contents were higher in Brinjal with 44.32 mg g-1 whereas Luffa and Bitter gourd possess the Flavonoid concentration in the range 38.02 and 34.64mg g-1 respectively, the total tannin contents also higher in Brinjal 31.40 mg/g follwed by in Luffa and Bitter gourd with 25.17 and 21.19 mg/g respectively. Antimicrobial activity showed that, all the extracts are the highly effective against S. aureus as compared to E. coli. Finally, it is concluded that all the selected vegetables are very good sources of Phenolic compounds as well as phytochemicals and should be included in the daily human diet for good health. On the basis of obtained results, it is also suggested that these samples will be further investigated for the determination of fatty acids by GC-MS and liquid chromatography-mass spectrum (LC-MS).

FACTORS AFFECTING WHEAT YIELD VARIATION OF SMALLHOLDER FARMERS IN PUNJAB PROVINCE: APPLICATION OF ORDERED LOGISTIC MODEL

Many socioeconomic, institutional and biophysical factors are causing high wheat yield variation among wheat growers in the country in general and in the Punjab province in specific. Ultimate purpose of present study was to determine factors affecting probability of wheat yield being in low, medium or high ordinals given the set of yield changing inputs. Cross-sectional data collected from randomly selected 320 wheat growers with 80 respondents from each of four agro-ecological regions of Punjab Province was analyzed through proportional odds model to obtain the study objectives. The study found out some socio-economic and agro-ecology related variables such as age, tractor ownership, role of income diversification through part-time farming, the contribution of smallholder tenants and owner-cum-tenants and chemical fertilizers that can significantly affect wheat yield categories of low, medium or high. Based on findings, it is imperative to support young innovative farmers having their own farm machinery, generate off-farm/on-farm income generating avenues for part-time farmers, and provide more facilities to smallholder farmers of both tenants as well as owner-cum tenant class in enhancing their wheat production of higher level. Moreover, agriculture advisory services should focus more on the cotton-wheat zone accompanied by appropriate use of seed rate, chemical fertilizers, and plant protection measures to enhance wheat yield in Punjab Province.

EFFICIENCY OF ORGANIC AND INORGANIC PESTICIDES AGAINST STORED GRAIN INSECT PESTS

The insect pests cause substantial loss of grains in the field and during storage of food grains that worth millions of rupees. Hence, in order to manage stored grain insect pests a combination of organic and inorganic pesticide control materials was selected to check the efficiency against red flour beetle (Tribolium castaneum) and pulse beetle (Callosobruchus chinensis). Total 10 treatments were used, eight different organic bio-pesticides (neem seed, neem leaves, naswar, tobacco, eucalyptus, citrus, dhatura and mint) and one inorganic (naphthalene balls) and control used at 32 ± 2°C and 70 ± 5% RH. The results indicated that three doses of all treatments were used as 5, 10 and 20gm in 50gm grains. Overall, maximum (4.33 ± 1.00%) mortality of red flour beetle was observed using neem seed powder and no (0.0 0 ± 00%) mortality was observed in minte leaves powder. In case of pulse beetle, the maximum (5.07 ± 0.87%) mortality was observed using neem seed powder and minimum (0.66±0.22%) using citrus leaves powder. Maximum (16.26 ± 0.03) antifeedant efficiency of red flour beetle was observed in control group and minimum (6.46±0.65) antifeedant efficiency of red flour beetle was found in dhatura leaves powder at all tested concentrations. Maximum (14.23 ± 0.13) antifeedant efficiency of pulse beetle was determined in control group and and minimum (1.00 ± 0.04) was observed for naphthalene balls at all tested concentrations. Maximum (94.67%) repellency against red flour beetle was observed using neem seed powder and minimum (62.00%) was found in naphathalene balls as compared with their controls. Maximum (81.32%) repellency against pulse beetle was observed using naswar powder and minimum (63.34%) repellency was determined using neem leaves powder as compared with their control group. We concluded that 20% of neem seed powder concentration proved effective to suppress the population of both red flour beetle and pulse beetle.

ONION PRODUCTION AND MARKETING SYSTEM IN BALOCHISTAN PROVINCE

In Pakistan, onion is one of the most grown commercial crops in the various parts of the country. The study was carried out through a primary data collection from various stakeholders to examine the status of onion production in Balochistan province. The average per acre cost of production, productivity, profitability, input output ratio were analyzed. Furthermore, find out the issues and constraints faced by onion growers and recommend policy guidelines for sustainable onion production and marketing in the study area. Costs have been broken down in a variable, fixed and opportunity costs (depreciation and opportunity) costs for production factors that are owned by the onion growers. While the variable cost was high from other costs, among them seed rate cost was high in the variable cost and in the lowest costs was recorded in chemical weedicide from other variable costs and fixed cost was nil due to relief from Balochistan government, whereas total cost of production and net income was Rs.82737 and Rs.75665 per acre respectively found in the study area. Majority 67% seed dealers were reported that due to high ratio of population, more consumable, good seed quality, more benefit, short duration of time and more production of onion as the principal reason for increase seed suppliers over the past 5 years. Onion farmers must follow best methods in management and strive to adopt scientific guidance from local and other available sources, for example research stations, progressive producers and extension workers.

CONTROLLING OF FRUIT SPLITTING IN POMEGRANATE (PUNICA GRANATUM L.) BY FOLIAR APPLICATION OF GROWTH REGULATOR AND NUTRIENTS

Various growth regulators have been used to improve the quality of different fruit crops. Foliar spray of macro and micronutrients play an important role in vegetative growth, yield and fruit quality. In the present study, the influence of foliar application of growth regulators and nutrients on fruit splitting and fruit quality was evaluated. For that purpose, Naphthaleneacetic acid (NAA), Potassium nitrate (KNO3) and Boric acid (H3BO3) at the rate of 40 ppm, 1% and 0.3% were used respectively. Spray of chemicals were applied in 2nd and 8th week from full bloom to yield in pomegranate cultivar ‘Golden pearl’. The experiment was designed under Randomized Complete Block Design (RCBD) comprised with seven treatments and replicated thrice. Fruit splitting was reduced (48.68%) significantly with application of KNO3 + Boric acid, while maximum fruit size (60.26 cm2), fruit weight (84gm), fruit grain weight (136.38gm), total soluble solid (TSS) 12.52% and yield (21.9kg/plant) were observed in KNO3 + Boric acid. Moreover, peel weight was increased in control (60.66 gm) and minimum was observed in 48.62 gm in KNO3 + Boric acid. Finally, it is concluded KNO3 %+ Boric acid reveled best results against fruit splitting % and other fruit quality parameters. These findings show that application of KNO3+ Boric acid significantly influences fruit quality of pomegranate when fruit are in the beginning stages of growth and development.

IMPACT OF VARIOUS PRESERVATIVE SOLUTIONS ON VASE LIFE AND POST-HARVEST QUALITY OF CUT ROSES

Short vase life and post-harvest losses of cut flowers are major threat to floriculture industry. There are different preservative solutions that have been used to extend the post-harvest life of cut flowers. Hence, this study was executed at Floriculture laboratory of Ayub Agricultural Research Institute, Faisalabad during 2018-2020 to explore the efficacy of different preservative solutions on vase life and post-harvest quality of cut roses. There were 6 treatments viz, (T1= Distilled water, T2= Silver nitrate @ 100 ppm, T3= 8-Hydroxyquiroline citrate @ 100 ppm, T4= Sodium thiosulphate @100 ppm, T5= Sodium benzoate @100 ppm, T6= Sucrose @ 40 g/L) and two rose cultivars Kardinal and Gold medal. The experiment was arranged according to complete randomized design (CRD). Results designated that longest vase life (15 days), maximum soluble solid contents (9.3 Brix) and longest opening period (7.4 days) were acquired with silver nitrate @100 ppm solution while maximum flower size (6.77 cm2) and largest head diameter (8.6 cm) were achieved with sodium thiosulphate when applied at the rate of 100 ppm in cultivar Kardinal. All other chemical solutions also displayed positive effects. Keeping in view the remarkable impact of Silver nitrate on vase life and quality of cut roses, it is recommended for commercial growers and cut flower industry for preservation of cut roses for longer time.

ACHIEVING SUSTAINABLE RICE PRODUCTION WITH THE APPLICATION OF SUGARCANE INDUSTRIAL BY-PRODUCTS

Sugarcane industries generate a variety of by-products, which create disposal and environmental problems. The aim of current study was to find out the ways for utilization of these by-products in rice production. To elucidate this, a field trial was conducted at the research area (30.2°E; 71.5N°) of Bahauddin Zakariya University Multan, Pakistan. The calcareous soil with high pH (8.6) and low organic matter (0.5%) was used with following treatments: no input control (CK), only chemical fertilizer, nitrogen, phosphorus and potassium (NPK; CF), NPK+ Sugarcane Bagasse (SB), NPK+ Press Mud (PM), NPK+ Sugarcane Vinasse (SV), NPK+SB+PM (SB+PM), NPK+SB+SV (SB+SV), NPK+PM+SV (PM+SV), NPK+SB+PM+SV (SB+PM+SV). The results showed that SB significantly increased the plant height (16.3%), panicle length (9.3%), paddy yield (213.1%), and straw yield (189. 9%). Nutrient uptake was also improved, SB enhanced TN (324.6%), while the SB+PM increased the total phosphorus (318.2%), and total potassium (163.7%) contents in grains. Likewise, agronomic nutrient use efficiency was enhanced in SB treatment for N (274.2%), P (81.4%), and K (378.5%). Maximum net profit and benefit-cost ratio was observed in SB (93925 rupees; 0.54), followed by PM (91150; 0.53), SB+PM (85338; 0.51), and minimum was in control (12400; 0.22), respectively. The study concludes, application of organic by-products (SB+PM) can increase rice productivity and farm income with environmental safety.