scholarly journals Proteomic analysis of subarachnoid hemorrhage - liquid phase isoelectric focusing in complex protein sample

2016 ◽  
Vol 85 (3) ◽  
pp. 161
Author(s):  
Joanna Hajduk ◽  
Bartosz Sokół ◽  
Agata Swiatly ◽  
Jan Matysiak ◽  
Piotr Nowicki ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Subarachnoid Hemorrhage ◽  
Liquid Phase ◽  
Isoelectric Focusing ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Proteomic Approach ◽  
Intersection Analysis ◽  
New Perspective ◽  
Tof Ms

Background: The aim of this study was to present the proteomic approach based on liquid phase isoelectric focusing fractionation coupled to nLC-MALDI-TOF/TOF-MS/MS analysis to characterize cerebrospinal fluid from control patients and those suffering from subarachnoid hemorrhage. The new perspective in characterization of this brain neuropathology are in constant demand to point a valuable panel of indicators which could improve the treatment outcome.Methods: The cerebrospinal fluid samples were applied to a commercial liquid phase isoelectric focusing apparatus and separated into 10 fractions by pI. Further, the untargeted mass spectrometry investigations were performed with data dependent acquisition mode for full-scan MS analysis with subsequently MS/MS fragmentation by using nLC-MALDI-TOF/TOF-MS/MS.Results: In total, the detection of 1664 and 2187 unique tryptic peptides provided biological evidence for 134 and 271 proteins in control and subarachnoid hemorrhage sample, respectively. The interpretation of liquid phase separation was performed by intersection analysis of two items between groups of ten fractions. The cumulative intersection exploration revealed the highest concentration of the detected components in the middle fractions of the focusing chamber, whereas the gradual dilution appeared on its extreme.Conclusions: The employed strategy ensured overall screening of investigated material presenting the proteins abundance in the current state of analysis. Few proteins such as proenkephalin A, peroxiredoxin-6, cathepsin B, thrombospondin-1, glial fibrillary acidic protein and α – spectrin were recognized as potential indicators, according to literature, pointing the possibility for its monitoring in further studies as panel of valuable biomarkers. 

The suitability of different DHB isomers as matrices for the MALDI-TOF MS analysis of phospholipids: which isomer for what purpose?

2006 ◽  
Vol 36 (4-5) ◽  
pp. 517-527 ◽  
Author(s):  
Jürgen Schiller ◽  
Rosmarie Süß ◽  
Beate Fuchs ◽  
Matthias Müller ◽  
Marijana Petković ◽  
...  
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

NMR, HPLC-ESI-MS, and MALDI-TOF MS Analysis of Condensed Tannins from Delonix regia (Bojer ex Hook.) Raf. and Their Bioactivities

2012 ◽  
Vol 60 (19) ◽  
pp. 5013-5022 ◽  
Author(s):  
Wei-Ming Chai ◽  
Yan Shi ◽  
Hui-Ling Feng ◽  
Ling Qiu ◽  
Hai-Chao Zhou ◽  
...  
Keyword(s):  
Condensed Tannins ◽  
Maldi Tof Ms ◽  
Esi Ms ◽  
Delonix Regia ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

Identification of Lice Stored in Alcohol Using MALDI-TOF MS

2020 ◽  
Author(s):  
Hanene Benyahia ◽  
Basma Ouarti ◽  
Adama Zan Diarra ◽  
Mehdi Boucheikhchoukh ◽  
Mohamed Nadir Meguini ◽  
...  
Keyword(s):  
Economic Consequences ◽  
Correct Identification ◽  
Protein Profiles ◽  
Innovative Strategy ◽  
Maldi Tof Ms ◽  
Pediculus Humanus ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Storage Methods ◽  
Tof Ms

Abstract Lice pose major public and veterinary health problems with economic consequences. Their identification is essential and requires the development of an innovative strategy. MALDI-TOF MS has recently been proposed as a quick, inexpensive, and accurate tool for the identification of arthropods. Alcohol is one of the most frequently used storage methods and makes it possible to store samples for long periods at room temperature. Several recent studies have reported that alcohol alters protein profiles resulting from MS analysis. After preliminary studies on frozen lice, the purpose of this research was to evaluate the influence of alcohol preservation on the accuracy of lice identification by MALDI-TOF MS. To this end, lice stored in alcohol for variable periods were submitted for MS analysis and sample preparation protocols were optimized. The reproducibility and specificity of the MS spectra obtained on both these arthropod families allowed us to implement the reference MS spectra database (DB) with protein profiles of seven lice species stored in alcohol. Blind tests revealed a correct identification of 93.9% of Pediculus humanus corporis (Linnaeus, 1758) and 98.4% of the other lice species collected in the field. This study demonstrated that MALDI-TOF MS could be successfully used for the identification of lice stored in alcohol for different lengths of time.

Identification of Arcanobacterium hippocoleae by MALDI-TOF MS analysis and by various genotypical properties

2017 ◽  
Vol 115 ◽  
pp. 10-12 ◽  
Author(s):  
J.-P. Wickhorst ◽  
O. Sammra ◽  
A.A. Hassan ◽  
M. Alssashen ◽  
C. Lämmler ◽  
...  
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

Development of magnetic LuPO4 microspheres for highly selective enrichment and identification of phosphopeptides for MALDI-TOF MS analysis

2015 ◽  
Vol 3 (48) ◽  
pp. 9330-9339 ◽  
Author(s):  
Xing-yu Long ◽  
Qun Song ◽  
Hong-zhen Lian
Keyword(s):  
Core Shell ◽  
Maldi Tof Ms ◽  
Selective Enrichment ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

Lichee-like core–shell structured magnetic lutetium phosphate (Fe3O4@LuPO4) affinity microspheres were synthesized, characterized and successfully applied to enrich phosphopeptides.

Finding diabetic nephropathy biomarkers in the plasma peptidome by high-throughput magnetic bead processing and MALDI-TOF-MS analysis

2010 ◽  
Vol 4 (8-9) ◽  
pp. 697-705 ◽  
Author(s):  
Henning G. Hansen ◽  
Julie Overgaard ◽  
Maria Lajer ◽  
Frantisek Hubalek ◽  
Peter Højrup ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
High Throughput ◽  
Magnetic Bead ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

scholarly journals N-(1-Naphthyl) Ethylenediamine Dinitrate: A New Matrix for Negative Ion MALDI-TOF MS Analysis of Small Molecules

2012 ◽  
Vol 23 (9) ◽  
pp. 1454-1460 ◽  
Author(s):  
Rui Chen ◽  
Suming Chen ◽  
Caiqiao Xiong ◽  
Xunlei Ding ◽  
Chih-Che Wu ◽  
...  
Keyword(s):  
Small Molecules ◽  
Negative Ion ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms
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