scholarly journals NANOG Plays a Hierarchical Role in the Transcription Network Regulating the Pluripotency and Plasticity of Adipose Tissue-Derived Stem Cells (ASCs)

2017 ◽  
Author(s):  
Maria Pitrone ◽  
Giuseppe Pizzolanti ◽  
Laura Tomasello ◽  
Antonina Coppola ◽  
Lorenzo Morini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Markers ◽  
Transcription Network ◽  
Cell Markers ◽  
Nanog Gene ◽  
Subcutaneous Adipose

The stromal vascular cell fraction (SVF) of visceral and subcutaneous adipose tissue (VAT and SAT) has increasingly come into focus in stem cell research, since these compartments represent a rich source of multipotent adipose-derived stem cells (ASCs). ASCs exhibit a self- renewal potential and differentiation capacity. Our aim was to study the different expression of embryonic stem cell markers NANOG, SOX2 and OCT3/4 and to evaluate if there exists a hierarchal role in this network in ASCs derived from both SAT and VAT. ASCs were isolated from SAT and VAT biopsies of 72 consenting patients (23 men, 47 women; age 45 ± 10; BMI between 25 and 30 range) undergoing elective open-abdominal surgery. Sphere-forming capability was evaluated by plating cells in low adhesion plastic. Stem cell markers CD90 and CD105 were analyzed by flow cytometry and stem cell transcription factors NANOG, SOX2 and OCT3/4 were detected by immunoblotting and Real-Time PCR. NANOG, SOX2 and OCT3/4 interplay was explored by gene silencing. ASCs from VAT and SAT confirmed their mesenchymal stem cell (MSC) phenotype expressing the specific MSC markers CD90, CD105, NANOG, SOX2 and OCT3/4. NANOG silencing induced a significant OCT 3/4 (70% ± 0.05) and SOX2 (75% ± 0.03) down-regulation whereas SOX2 silencing did not affect NANOG gene expression. Adipose tissue is an important source of MSC, and siRNA experiments endorse a hierarchical role of NANOG in the complex transcription network that regulates pluripotency and plasticity.

scholarly journals NANOG Plays a Hierarchical Role in the Transcription Network Regulating the Pluripotency and Plasticity of Adipose Tissue-Derived Stem Cells (ASCs)

2017 ◽  
Author(s):  
Maria Pitrone ◽  
Giuseppe Pizzolanti ◽  
Laura Tomasello ◽  
Antonina Coppola ◽  
Lorenzo Morini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Markers ◽  
Transcription Network ◽  
Cell Markers ◽  
Nanog Gene ◽  
Subcutaneous Adipose

The stromal vascular cell fraction (SVF) of visceral and subcutaneous adipose tissue (VAT and SAT) has increasingly come into focus in stem cell research, since these compartments represent a rich source of multipotent adipose-derived stem cells (ASCs). ASCs exhibit a self- renewal potential and differentiation capacity. Our aim was to study the different expression of embryonic stem cell markers NANOG, SOX2 and OCT3/4 and to evaluate if there exists a hierarchal role in this network in ASCs derived from both SAT and VAT. ASCs were isolated from SAT and VAT biopsies of 72 consenting patients (23 men, 47 women; age 45 ± 10; BMI between 25 and 30 range) undergoing elective open-abdominal surgery. Sphere-forming capability was evaluated by plating cells in low adhesion plastic. Stem cell markers CD90 and CD105 were analyzed by flow cytometry and stem cell transcription factors NANOG, SOX2 and OCT3/4 were detected by immunoblotting and Real-Time PCR. NANOG, SOX2 and OCT3/4 interplay was explored by gene silencing. ASCs from VAT and SAT confirmed their mesenchymal stem cell (MSC) phenotype expressing the specific MSC markers CD90 and CD105 and NANOG, SOX2 and OCT3/4. NANOG silencing induced a significant OCT 3/4 (70% ± 0.05) and SOX2 (75% ± 0.03) down-regulation whereas SOX2 silencing did not affect NANOG gene expression. Adipose tissue is an important source of MSC, and siRNA experiments endorse a hierarchical role of NANOG in the complex transcription network that regulates pluripotency and plasticity.

scholarly journals Detection of embryonic stem cell markers in adult human adipose tissue-derived stem cells

2011 ◽  
Vol 54 (3) ◽  
pp. 501 ◽  
Author(s):  
SarasaBharati Arumugam ◽  
OmanaA Trentz ◽  
Devi Arikketh ◽  
Vijayalakshmi Senthinathan ◽  
Barry Rosario ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Human Adipose Tissue ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Adult Human ◽  
Embryonic Stem Cell Markers

scholarly journals Characterization of adipose tissue macrophages and adipose-derived stem cells in critical wounds

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e2824 ◽  
Author(s):  
Bong-Sung Kim ◽  
Pathricia V. Tilstam ◽  
Katrin Springenberg-Jung ◽  
Arne Hendrick Boecker ◽  
Corinna Schmitz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Subcutaneous Adipose Tissue ◽  
Stem Cell Markers ◽  
Adipose Derived Stem Cells ◽  
Cell Markers ◽  
Adipose Tissue Macrophages ◽  
Subcutaneous Adipose

Background Subcutaneous adipose tissue is a rich source of adipose tissue macrophages and adipose-derived stem cells which both play a key role in wound repair. While macrophages can be divided into the classically-activated M1 and the alternatively-activated M2 phenotype, ASCs are characterized by the expression of specific stem cell markers. Methods In the present study, we have investigated the expression of common macrophage polarization and stem cell markers in acutely inflamed adipose tissue. Subcutaneous adipose tissue adjacent to acutely inflamed wounds of 20 patients and 20 healthy subjects were harvested and underwent qPCR and flow cytometry analysis. Results Expression levels of the M1-specific markers CD80, iNOS, and IL-1b were significantly elevated in inflammatory adipose tissue when compared to healthy adipose tissue, whereas the M2-specific markers CD163 and TGF-β were decreased. By flow cytometry, a significant shift of adipose tissue macrophage populations towards the M1 phenotype was confirmed. Furthermore, a decrease in the mesenchymal stem cell markers CD29, CD34, and CD105 was observed whereas CD73 and CD90 remained unchanged. Discussion This is the first report describing the predominance of M1 adipose tissue macrophages and the reduction of stem cell marker expression in acutely inflamed, non-healing wounds.

Stress resistant human embryonic stem cells as a potential source for the identification of novel cancer stem cell markers

2010 ◽  
Vol 289 (2) ◽  
pp. 208-216 ◽  
Author(s):  
Shaker A. Mousa ◽  
Thangirala Sudha ◽  
Evgeny Dyskin ◽  
Usawadee Dier ◽  
Christine Gallati ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Cancer Stem Cell ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Cancer Stem Cell Markers ◽  
Potential Source

scholarly journals Stem Cell and Markers

2019 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Adult Stem Cells ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Wide Range

Stem cells have the ability to go through various cell divisions and also maintain undifferentiated state. Stem cells are Embryonic (Pluripotent) and adult stem cells. Pluripotent stem cells give rise to all tissues such as ectoderm, mesoderm and endoderm. Embryonic stem cells isolated from inner cell mass of embryo blastocyst. Adult stem cells are also undifferentiated cells present in adult organisms and repair the tissue when damaged occurs but number in less. Adult stem cells are present in bone marrow, adipose tissue, blood and juvenile state umbilical cord and tissue of specific origin like liver, heart, intestine and neural tissue. Embryonic stem cells from blastocyst have the ethical problems and tumorogenecity. These can be identified by flow cytometry. There are wide range of stem cell markers which are useful in identifying them. Most of the pluripotent cell markers are common with tumor cell markers which throws a challenge for certainty.

scholarly journals Assessment of Stem Cell Markers During Long-Term Culture of Mouse Embryonic Stem Cells

2004 ◽  
Vol 44 (1/2) ◽  
pp. 77-91 ◽  
Author(s):  
A. Berrill ◽  
H.L. Tan ◽  
S.C. Wuang ◽  
W.J. Fong ◽  
Andre B.H. Choo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Long Term Culture

scholarly journals Comparison of Immuno-Phenotypes of Stem Cells from Human Dental Pulp and Periodontal Ligament

2012 ◽  
Vol 25 (1) ◽  
pp. 127-134 ◽  
Author(s):  
D. Ponnaiyan ◽  
K.M. Bhat ◽  
G.S. Bhat
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Population ◽  
Dental Pulp ◽  
Periodontal Ligament ◽  
Embryonic Stem ◽  
Stem Cell Population ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Human Dental Pulp

It has been established that human dental pulp and periodontal ligament contain a population of mesenchymal stem cells (MSCs). However, the phenotypic analysis in terms of putative stem cell markers expressed by these stem cell populations is incomplete. It is relevant to understand whether stem cells derived from closely related tissues are programmed differently. The aim of the present study is to analyze whether these stem cells depict distinct characteristics by gaining insight into differences in their immunophenotype. Dental pulp and periodontal ligament tissue samples were obtained from extracted impacted wisdom teeth. Cell cultures were analyzed for surface and intracellular markers by indirect immunoflourescence. Detailed immunophenotype analysis was carried out by flow cytometry using relevant markers. The present study data shows dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) expressed embryonic stem (ES) cell markers Oct-4, Nanog and mesodermal marker Vimentin by indirect immunoflourescence. PDLSCs, however, had a weak expression of Nanog. Immunophenotyping revealed strong expression of MSC markers (CD73, CD90) in DPSCs and PDLSCs. Differences were observed in expression of sternness-related markers. DPSCs displayed increased percentages of SSEA4, CD13 and CD166 and decreased CD9 expression compared to PDLSCs. Both stem cells express common MSC markers, different levels of expression suggests there might be more than one stem cell population existing within these tissues which differ in their embryonic status, and DPSCs are a more primitive stem cell population in comparison to PDLSCs.

scholarly journals Stem cell markers expression evaluation in Oral Squamous Cell Carcinoma

2021 ◽  
Vol 10 (13) ◽  
pp. e314101320840
Author(s):  
Julio Cesar Ramos Cadilho ◽  
Silvia Maria de Carvalho Lyra ◽  
Ana Beatriz Machado Lima ◽  
Carina Maciel da Silva Boghossian ◽  
Cláudia Maria Pereira
Keyword(s):  
Stem Cells ◽  
Squamous Cell Carcinoma ◽  
Stem Cell ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Embryonic Stem ◽  
Tobacco Consumption ◽  
Stem Cell Markers ◽  
Cell Markers

Oral carcinogenesis is a highly complex process. Molecular studies demonstrate that some oral cancers progress from pre-neoplastic lesions. Cancer stem cells (CSCs), a small cellular group within the tumor, have currently received much attention by its self-renewal and tumor differentiation ability. It is considered that CSCs may be responsible for the relapse and metastasis of cancer. CSCs have been reported to express common embryonic stem cells markers such as Nestin and Nanog. The aim of the present study was to identify the presence of Nestin and Nanog markers in patients with oral squamous cell carcinoma (OSCC). Nine samples were collected from patients diagnosed with OSCC and two healthy oral mucosa were used as control. The markers expression in nine OSCC was determined by end-point PCR. The OSCC population analyzed was mostly composed of black and white males, with a mean age of 70.6 years, that reported alcohol and tobacco consumption. Nestin and Nanog stem cell markers were expressed in all OSCCs and normal samples analyzed. As these two markers have already been related to the mechanism of metastasis and relapses in cancer, its expression determination could help to understand the aggressive and invasive nature of OSCC.

Isolation and Characterization of a Population of Immature Dental Pulp Stem Cells Expressing OCT-4 and Other Embryonic Stem Cell Markers

2006 ◽  
Vol 184 (3-4) ◽  
pp. 105-116 ◽  
Author(s):  
Irina Kerkis ◽  
Alexandre Kerkis ◽  
Dmitri Dozortsev ◽  
Gaëlle Chopin Stukart-Parsons ◽  
Sílvia Maria Gomes Massironi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Dental Pulp ◽  
Embryonic Stem ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Isolation And Characterization ◽  
Embryonic Stem Cell Markers

scholarly journals Upregulation of Pluripotency Markers in Adipose Tissue-Derived Stem Cells by miR-302 and Leukemia Inhibitory Factor

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Masoumeh Fakhr Taha ◽  
Arash Javeri ◽  
Sara Rohban ◽  
Seyed Javad Mowla
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Leukemia Inhibitory Factor ◽  
Culture Media ◽  
Embryonic Stem ◽  
Inhibitory Factor ◽  
Stem Cell Markers ◽  
Pluripotency Markers

The expression pattern of pluripotency markers in adipose tissue-derived stem cells (ADSCs) is a subject of controversy. Moreover, there is no data about the signaling molecules that regulate these markers in ADSCs. In the present study, we studied the roles of leukemia inhibitory factor (LIF) and miR-302 in this regard. Freshly isolated mouse ADSCs expressed hematopoietic, mesenchymal, and pluripotency markers. One day after plating, ADSCs expressed OCT4 and Sox2 proteins. After three passages, the expression of hematopoietic and pluripotency markers decreased, while the expression of mesenchymal stem cell markers exhibited a striking rise. Both supplementation of culture media with LIF and transfection of the ADSCs with miR-302 family upregulated the expression levels ofOCT4,Nanog, andSox2mRNAs. These findings showed that mouse adipose tissue contains a population of cells with molecular resemblance to embryonic stem cells, and LIF and miR-302 family positively affect the expression of pluripotency markers.

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