Inhibition of Cyclooxygenase-2 Alters Cranial Neural Crest Cell Migration in the Developing chick

A recent study from our lab revealed that inhibition of cyclooxygenase-2 exclusively reduces the level of PGE2 amongst the prostanoids and hamper the normal development of several structures, strikingly the cranial vault, in chick embryos. In order to unearth the mechanism behind the deviant development of cranial features, the expression pattern of various factors that are known to influence the cranial neural crest cell (CNCC) migration were checked in chick embryo after inhibiting the COX-2 activity using etoricoxib. The compromised level of cell adhesion molecules and their upstream regulators, namely CDH1, CDH2, MSX1, and TGF-β, observed in the etoricoxib treated embryos indicate that COX-2, through its downstream effector PGE2, regulates the expression of these factors perhaps to aid the migration of CNCC. The histological features and levels of FoxD3 as well as PCNA further consolidates the role of COX-2 in migration and survival of CNCC in developing embryo. The results of the current study indicate that the COX-2 plays a pivotal role in orchestrating the proliferation and migration of CNCC during embryonic development of chick.

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Faculty Opinions recommendation of Leader cells define directionality of trunk, but not cranial, neural crest cell migration.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726375426.793519028 ◽

2016 ◽

Author(s):

Eric Theveneau

Keyword(s):

Cell Migration ◽

Neural Crest ◽

Neural Crest Cell ◽

Cranial Neural Crest ◽

Cranial Neural Crest Cell ◽

Neural Crest Cell Migration ◽

Crest Cell

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Proliferation dynamics associated with cranial neural crest cell migration

Developmental Biology ◽

10.1016/j.ydbio.2011.05.266 ◽

2011 ◽

Vol 356 (1) ◽

pp. 197

Author(s):

Dennis A. Ridenour ◽

Rebecca McLennan ◽

Jessica M. Teddy ◽

Katherine W. Prather ◽

Craig L. Semerad ◽

...

Keyword(s):

Cell Migration ◽

Neural Crest ◽

Neural Crest Cell ◽

Cranial Neural Crest ◽

Cranial Neural Crest Cell ◽

Neural Crest Cell Migration ◽

Crest Cell

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Inhibition of cranial neural crest cell development by vitamin A in the cultured chick embryo

Development ◽

10.1242/dev.39.1.267 ◽

1977 ◽

Vol 39 (1) ◽

pp. 267-271

Author(s):

John R. Hassell ◽

Judith H. Greenberg ◽

Malcolm C. Johnston

Keyword(s):

Vitamin A ◽

Neural Crest ◽

Neural Crest Cell ◽

Cell Development ◽

Cranial Neural Crest ◽

Cranial Neural Crest Cell ◽

Neural Crest Cell Migration ◽

Cranial Neural Crest Cells ◽

Crest Cell ◽

Whole Egg

Chick embryos at stage 8, prior to neural crest cell migration, were explanted on whole egg medium with or without vitamin A and cultured for 3 days. Sections through the head regions showed that the cranial neural crest cells had migrated into the first visceral arch in the controls but were absent from this structure in the treated embryos. These observations suggest that vitamin A inhibits neural crest cell development or migration, an effect which may in part account for the facial malformations produced by excess vitamin A.

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The Wnt Co-Receptor Lrp5 Is Required for Cranial Neural Crest Cell Migration in Zebrafish

PLoS ONE ◽

10.1371/journal.pone.0131768 ◽

2015 ◽

Vol 10 (6) ◽

pp. e0131768 ◽

Cited By ~ 20

Author(s):

Bernd Willems ◽

Shijie Tao ◽

Tingsheng Yu ◽

Ann Huysseune ◽

Paul Eckhard Witten ◽

...

Keyword(s):

Cell Migration ◽

Neural Crest ◽

Neural Crest Cell ◽

Cranial Neural Crest ◽

Cranial Neural Crest Cell ◽

Neural Crest Cell Migration ◽

Crest Cell

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Signalling between the hindbrain and paraxial tissues dictates neural crest migration pathways

Development ◽

10.1242/dev.129.2.433 ◽

2002 ◽

Vol 129 (2) ◽

pp. 433-442 ◽

Cited By ~ 3

Author(s):

Paul A. Trainor ◽

Dorothy Sobieszczuk ◽

David Wilkinson ◽

Robb Krumlauf

Keyword(s):

Cell Migration ◽

Neural Crest ◽

Neural Crest Cell ◽

Neural Crest Cells ◽

Cranial Neural Crest ◽

Branchial Arches ◽

Neural Crest Cell Migration ◽

And Migration ◽

Migration Pathways ◽

Crest Cell

Cranial neural crest cells are a pluripotent population of cells derived from the neural tube that migrate into the branchial arches to generate the distinctive bone, connective tissue and peripheral nervous system components characteristic of the vertebrate head. The highly conserved segmental organisation of the vertebrate hindbrain plays an important role in pattering the pathways of neural crest cell migration and in generating the distinct or separate streams of crest cells that form unique structures in each arch. We have used focal injections of DiI into the developing mouse hindbrain in combination with in vitro whole embryo culture to map the patterns of cranial neural crest cell migration into the developing branchial arches. Our results show that mouse hindbrain-derived neural crest cells migrate in three segregated streams adjacent to the even-numbered rhombomeres into the branchial arches, and each stream contains contributions of cells from three rhombomeres in a pattern very similar to that observed in the chick embryo. There are clear neural crest-free zones adjacent to r3 and r5. Furthermore, using grafting and lineage-tracing techniques in cultured mouse embryos to investigate the differential ability of odd and even-numbered segments to generate neural crest cells, we find that odd and even segments have an intrinsic ability to produce equivalent numbers of neural crest cells. This implies that inter-rhombomeric signalling is less important than combinatorial interactions between the hindbrain and the adjacent arch environment in specific regions, in the process of restricting the generation and migration of neural crest cells. This creates crest-free territories and suggests that tissue interactions established during development and patterning of the branchial arches may set up signals that the neural plate is primed to interpret during the progressive events leading to the delamination and migration of neural crest cells. Using interspecies grafting experiments between mouse and chick embryos, we have shown that this process forms part of a conserved mechanism for generating neural crest-free zones and contributing to the separation of migrating crest populations with distinct Hox expression during vertebrate head development.

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