scholarly journals Piezoelectric Immunosensor for the Determination of Immunoglobulin G

2018 ◽  
pp. 8784-8791 ◽  
Author(s):  
Miroslav Pohanka ◽  
Keyword(s):  
Immunoglobulin G ◽  
Piezoelectric Immunosensor

Determination of immunoglobulin G in mare colostrum by high-performance gel permeation chromatography

2011 ◽  
Vol 29 (3) ◽  
pp. 265-268
Author(s):  
Yuewen LU ◽  
Hongjuan WANG ◽  
Jie YANG
Keyword(s):  
Immunoglobulin G ◽  
High Performance ◽  
Gel Permeation Chromatography ◽  
Gel Permeation

Microparticle enzyme immunoassay for determination of immunoglobulin G antibodies to human cytomegalovirus.

1988 ◽  
Vol 26 (10) ◽  
pp. 2229-2230 ◽  
Author(s):  
G Duverlie ◽  
C Roussel ◽  
M Driencourt ◽  
J Orfila
Keyword(s):  
Enzyme Immunoassay ◽  
Human Cytomegalovirus ◽  
Immunoglobulin G ◽  
Microparticle Enzyme Immunoassay

Automated particle-counting immunoassay for alpha-fetoprotein.

1981 ◽  
Vol 27 (1) ◽  
pp. 64-67 ◽  
Author(s):  
D Collet-Cassart ◽  
C G Magnusson ◽  
J G Ratcliffe ◽  
C L Cambiaso ◽  
P L Masson
Keyword(s):  
Correlation Coefficient ◽  
Immunoglobulin G ◽  
Incubation Time ◽  
Blood Cells ◽  
Sampling Rate ◽  
Alpha Fetoprotein ◽  
Optical Cell ◽  
Standard Curve ◽  
Cell Counter

Abstract PACIA, a homogeneous non-radioimmunoassay, has been adapted to the determination of serum alpha 1-fetoprotein. This technique is based on the agglutination of latex particles coated with antibodies to the antigen to be determined. The agglutination is measured by using an optical cell counter designed to count blood cells, to determine the reduction in the number of non-agglutinated particles. Interferences by serum constituents are avoided by coating the particles with the F(ab')2-fragments of th immunoglobulin G fraction of the antiserum. The system is automated, with a sampling rate of 50/h and an incubation time of 26 min. Concentrations used in preparing the standard curve ranged from 1 to 50 microgram/L; analytical recoveries were 93.5 to 98.4%; the correlation coefficient of PACIA with radioimmunoassay, calculated from results on 127 samples, was 0.98; maximum within- and between-assay CVs were 7.4% and 9.6%, respectively.

scholarly journals Allelic Diversity of the Two Transferrin Binding Protein B Gene Isotypes among a Collection of Neisseria meningitidisStrains Representative of Serogroup B Disease: Implication for the Composition of a Recombinant TbpB-Based Vaccine

2000 ◽  
Vol 68 (9) ◽  
pp. 4938-4947 ◽  
Author(s):  
Bachra Rokbi ◽  
Geneviève Renauld-Mongenie ◽  
Michèle Mignon ◽  
B. Danve ◽  
David Poncet ◽  
...  
Keyword(s):  
Amino Acids ◽  
Bactericidal Activity ◽  
Immunoglobulin G ◽  
Binding Protein ◽  
Restriction Analysis ◽  
Allelic Diversity ◽  
Gene Variants ◽  
C Terminus ◽  
Specific Antisera

ABSTRACT The distribution of the two isotypes of tbpB in a collection of 108 serogroup B meningococcal strains belonging to the four major clonal groups associated with epidemic and hyperendemic disease (the ET-37 complex, the ET-5 complex, lineage III, and cluster A4) was determined. Isotype I strains (with a 1.8-kbtbpB gene) was less represented than isotype II strains (19.4 versus 80.6%). Isotype I was restricted to the ET-37 complex strains, while isotype II was found in all four clonal complexes. The extent of the allelic diversity of tbpB in these two groups was studied by PCR restriction analysis and sequencing of 10 newtbpB genes. Four major tbpB gene variants were characterized: B16B6 (representative of isotype I) and M982, BZ83, and 8680 (representative of isotype II). The relevance of these variants was assessed at the antigenic level by the determination of cross-bactericidal activity of purified immunoglobulin G preparations raised to the corresponding recombinant TbpB (rTbpB) protein against a panel of 27 strains (5 of isotype I and 22 of isotype II). The results indicated that rTbpB corresponding to each variant was able to induce cross-bactericidal antibodies. However, the number of strains killed with an anti-rTbpB serum was slightly lower than that obtained with an anti-TbpA+B complex. None of the sera tested raised against an isotype I strain was able to kill an isotype II strain and vice versa. None of the specific antisera tested (anti-rTbpB or anti-TbpA+B complex) was able to kill all of the 22 isotype II strains tested. Moreover, using sera raised against the C-terminus domain of TbpB M982 (amino acids 352 to 691) or BZ83 (amino acids 329 to 669) fused to the maltose-binding protein, cross-bactericidal activity was detected against 12 and 7 isotype II strains, respectively, of the 22 tested. These results suggest surface accessibility of the C-terminal end of TbpB. Altogether, these results show that although more than one rTbpB will be required in the composition of a TbpB-based vaccine to achieve a fully cross-bactericidal activity, rTbpB and its C terminus were able by themselves to induce cross-bactericidal antibodies.

Determination of anti–Toxoplasma gondii immunoglobulin G avidity: adaptation to the vidas system (bioMérieux)

1998 ◽  
Vol 32 (2) ◽  
pp. 69-73 ◽  
Author(s):  
Hervé Pelloux ◽  
Emmanuelle Brun ◽  
Guy Vernet ◽  
Suzanne Marcillat ◽  
Michel Jolivet ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunoglobulin G

scholarly journals Development of a Rapid and Convenient Method for Determination of Rubella Virus-Specific Immunoglobulin G Avidity

2007 ◽  
Vol 14 (11) ◽  
pp. 1416-1419 ◽  
Author(s):  
Christelle Vauloup-Fellous ◽  
Jessica Ursulet-Diser ◽  
Liliane Grangeot-Keros
Keyword(s):  
Immunoglobulin G ◽  
Rubella Virus ◽  
Primary Infection ◽  
Virus Infections ◽  
Serum Samples ◽  
Igg Avidity ◽  
Specific Immunoglobulin ◽  
Specific Igg ◽  
Semiautomated Method

ABSTRACT We describe here a rapid and semiautomated method for the determination of rubella virus immunoglobulin G (IgG) avidity with the VIDAS instrument. A total of 153 serum samples from persons with naturally acquired rubella virus infections (n = 98), from vaccinated persons (n = 44), and from patients with autoantibodies (n = 11) were included in this study. The rubella virus-specific IgG avidity assay we developed for the VIDAS instrument was evaluated by comparison with an in-house method. Results obtained with the VIDAS instrument allow considering this method valuable to help confirm or exclude acute primary infection or recent vaccination.

scholarly journals Development of a Fluorescent-Bead-Based Multiplex Immunoassay To Determine Immunoglobulin G Subclass Responses to Neisseria meningitidis Serogroup A and C Polysaccharides

2008 ◽  
Vol 15 (8) ◽  
pp. 1188-1193 ◽  
Author(s):  
Richarda M. de Voer ◽  
Fiona R. M. van der Klis ◽  
Carla W. A. M. Engels ◽  
Ger T. Rijkers ◽  
Elisabeth A. Sanders ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
Immunoglobulin G ◽  
Serum Immunoglobulin ◽  
Igg Subclass ◽  
Fluorescent Particle ◽  
Multiplex Immunoassay ◽  
Specific Assay ◽  
Flow Cytometric ◽  
Immunoglobulin G Subclass

ABSTRACT A fluorescent-particle-based multiplex flow cytometric immunoassay (MIA) for the detection of serum immunoglobulin G (IgG) and two IgG subclasses, IgG1 and IgG2, specific for Neisseria meningitidis serogroup A (MenA) and C (MenC) polysaccharides (PS) was developed. The assay comprised three separate duplex assays, one for the detection of the IgG response to MenA and MenC PS, another for the detection of the IgG1 response to MenA and MenC PS, and a third for the detection of the IgG2 response to MenA and MenC PS. Next, the three separate duplex assays were combined and analyzed as a hexaplex assay. No interference between monoplex, duplex, and hexaplex assays was observed, and the assay was found to have low intra- and interassay variation (<9.0% and <27%, respectively). Comparison of the meningococcal subclass MIA to the in-house enzyme-linked inmmunosorbent assays showed a good correlation (R ≥ 0.85) for each of the subclasses. We conclude that the hexaplex meningococcal subclass MIA is an easy and specific assay for the determination of anti-MenA and anti-MenC PS subclass IgG, requiring minimal amounts of serum to study IgG subclass responses to vaccines.

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