Altered exosomal microRNA profiles in bronchoalveolar lavage fluid can mediate metabolism in patients with Acinetobacter baumannii ventilator-associated pneumonia

ABSTRACTAcinetobacter baumanniiis one of the main pathogens that cause ventilator-associated pneumonia (VAP) and is associated with a high rate of mortality. Little is known about the efficacy of macrolides againstA. baumannii. In order to confirm the efficacy of azithromycin (AZM) against VAP caused by multidrug-resistantA. baumannii(MDRAB), we used a mouse model that mimics VAP by placement of a plastic tube in the bronchus. AZM (10 and 100 mg/kg of body weight) was administered subcutaneously every 24 h beginning at 3 h after inoculation. Phosphate-buffered saline was administered as the control. Survival was evaluated over 7 days. At 48 h postinfection, mice were sacrificed and the numbers of viable bacteria in lungs and bronchoalveolar lavage fluid were compared. Histopathological analysis of lung specimens was also performed. The treatment groups displayed significantly longer survival than the control group (P< 0.05). AZM did not have an antimicrobial effect. Histopathological examination of lung specimens indicated that the progression of lung inflammation was prevented in the AZM-treated groups. Furthermore, total cell and neutrophil counts, as well as cytokine levels, in bronchoalveolar lavage fluid were significantly decreased (P< 0.05) in the AZM-treated groups. AZM may have a role for the treatment of VAP with MDRAB because of its anti-inflammatory effects.

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Plasminogen activation inhibitor concentrations in bronchoalveolar lavage fluid distinguishes ventilator-associated pneumonia from colonization in mechanically ventilated pediatric patients

Pediatric Critical Care Medicine ◽

10.1097/pcc.0b013e3181e2a352 ◽

2011 ◽

Vol 12 (1) ◽

pp. 21-27 ◽

Cited By ~ 14

Author(s):

Ramya Srinivasan ◽

Yuanlin Song ◽

Jeanine Wiener-Kronish ◽

Heidi R. Flori

Keyword(s):

Bronchoalveolar Lavage ◽

Bronchoalveolar Lavage Fluid ◽

Pediatric Patients ◽

Lavage Fluid ◽

Ventilator Associated Pneumonia ◽

Plasminogen Activation ◽

Mechanically Ventilated

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Ventilator-associated pneumonia and atelectasis: evaluation through bronchoalveolar lavage fluid analysis

Intensive Care Medicine ◽

10.1007/s00134-003-1680-8 ◽

2003 ◽

Vol 29 (4) ◽

pp. 555-563 ◽

Cited By ~ 33

Author(s):

G. Nakos ◽

H. Tsangaris ◽

S. Liokatis ◽

E. Kitsiouli ◽

M. E. Lekka

Keyword(s):

Bronchoalveolar Lavage ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid ◽

Ventilator Associated Pneumonia ◽

Fluid Analysis

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Evaluation of a Loop-Mediated Isothermal Amplification assay to detect carbapenemases from Acinetobacter spp. directly from bronchoalveolar lavage fluid

10.1101/2020.05.11.089904 ◽

2020 ◽

Author(s):

J. Moreno-Morales ◽

A. Vergara ◽

T. Kostyanev ◽

J. Rodriguez-Baño ◽

H. Goossens ◽

...

Keyword(s):

Bronchoalveolar Lavage ◽

Acinetobacter Baumannii ◽

Nosocomial Infections ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid ◽

Isothermal Amplification ◽

Loop Mediated Isothermal Amplification ◽

Carbapenem Resistant ◽

Amplification Assay ◽

Acinetobacter Spp

AbstractCarbapenem-resistant Acinetobacter spp. mainly Acinetobacter baumannii are frequently causing nosocomial infections with high mortality. In this study, the efficacy of the Eazyplex® SuperBug Complete A system (Amplex Diagnostics GmbH, Gars-Bahnhof, Germany), based on loop-mediated isothermal amplification (LAMP), to detect the presence of carbapenemases in Acinetobacter spp. directly from bronchoalveolar lavage samples was assessed, detecting all tested carbapenemases in less than 30 minutes with a sensitivity of 103 CFU/ml.

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