scholarly journals Comparative Morphometric Analysis of Experimental Plague Infection Caused by Yersinia pestis Strains with Different Genetic Characteristics

2008 ◽  
pp. 49-53 ◽  
Author(s):  
S. A. Bugorkova ◽  
V. E. Kouklev ◽  
T. V. Bugorkova ◽  
Z. V. Malykhina ◽  
V. V. Kutyrev
Keyword(s):  
Yersinia Pestis ◽  
Morphometric Analysis ◽  
Pulmonary Tissue ◽  
Genetic Characteristics ◽  
Infectious Process ◽  
Functional Systems ◽  
Process Formation ◽  
Immunocompetent Organs ◽  
Compensatory Process

Data on adaptive-compensatory process formation in detoxication and adaptation functional systems of test animals during the plague infection modeling were obtained by means of morphometric analysis. The latter included characterization of apudocytes condition in the number of organs. Changes of apudocytes activity and quantity in immunocompetent organs and pulmonary tissue of biomodels were determined. Morphometric indices selected for registration were shown to allow characterizing the severity of experimental infectious process.

scholarly journals Genetic characterization of skull base chondrosarcomas

2015 ◽  
Vol 123 (4) ◽  
pp. 1036-1041 ◽  
Author(s):  
Hiroki Kanamori ◽  
Yohei Kitamura ◽  
Tokuhiro Kimura ◽  
Kazunari Yoshida ◽  
Hikaru Sasaki
Keyword(s):  
Skull Base ◽  
Case Series ◽  
Bone Neoplasms ◽  
Comparative Genomic ◽  
Whole Genome ◽  
Whole Genome Analysis ◽  
Genetic Characteristics ◽  
Genome Study ◽  
Skull Base Chordomas

OBJECT Although chondrosarcomas rarely arise in the skull base, chondrosarcomas and chordomas are the 2 major malignant bone neoplasms occurring at this location. The distinction of these 2 tumors is important, but this distinction is occasionally problematic because of radiological and histological overlap. Unlike chordoma and extracranial chondrosarcoma, no case series presenting a whole-genome analysis of skull base chondrosarcomas (SBCSs) has been reported. The goal of this study is to clarify the genetic characteristics of SBCSs and contrast them with those of chordomas. METHODS The authors analyzed 7 SBCS specimens for chromosomal copy number alterations (CNAs) using comparative genomic hybridization (CGH). They also examined IDH1 and IDH2 mutations and brachyury expression. RESULTS In CGH analyses, the authors detected CNAs in 6 of the 7 cases, including chromosomal gains of 8q21.1, 19, 2q22-q32, 5qcen-q14, 8q21-q22, and 15qcen-q14. Mutation of IDH1 was found with a high frequency (5 of 7 cases, 71.4%), of which R132S was most frequently mutated. No IDH2 mutations were found, and immunohistochemical staining for brachyury was negative in all cases. CONCLUSIONS To the best of the authors' knowledge, this is the first whole-genome study of an SBSC case series. Their findings suggest that these tumors are molecularly consistent with a subset of conventional central chondrosarcomas and different from skull base chordomas.

scholarly journals Characterization of pulmonary cysts in Birt–Hogg–Dubé syndrome: histopathological and morphometric analysis of 229 pulmonary cysts from 50 unrelated patients

2014 ◽  
Vol 65 (1) ◽  
pp. 100-110 ◽  
Author(s):  
Toshio Kumasaka ◽  
Takuo Hayashi ◽  
Keiko Mitani ◽  
Hideyuki Kataoka ◽  
Mika Kikkawa ◽  
...  
Keyword(s):  
Morphometric Analysis ◽  
Pulmonary Cysts

scholarly journals Genotype Characterization of Human Hydatid Cyst Isolates From Patients in Karaj, Iran, Using COX1 Gene Sequence

2019 ◽  
Vol 7 (1) ◽  
pp. 27-29
Author(s):  
Maryam Salimi ◽  
Abolfazl Miahipour ◽  
Mohammad Zibaei ◽  
Sasan Rezaie
Keyword(s):  
Hydatid Cyst ◽  
Gene Sequence ◽  
Cox1 Gene ◽  
Zoonotic Infection ◽  
Pcr Assay ◽  
Genetic Characteristics ◽  
Control And Prevention ◽  
Polymerase Chain ◽  
Clinical Problems

Background: Cystic echinococcosis is a main zoonotic infection. It can cause serious clinical problems for human health around the world. Genotypic specification of Echinococcus granulosus in human is important due to control and prevention programs. Objective: In this investigation, genetic characteristics of human isolates of E. granulosus in Karaj, Iran, were studied. Materials and Methods: In this review, 3 isolates of surgically removed hydatid cysts were obtained from patients in Shahid Madani hospital, Karaj, Iran in 2014. DNA was extracted from the protoscolex of the cyst, and polymerase chain reaction (PCR) assay was done on the COX1 gene. Results: DNA fragments were sequenced and the results were aligned and analyzed. Among the isolates, 3 (100%) were E. granulosus (G1) strain. Conclusion: The G1 genotype was the most superior strain from human isolates of hydatid cyst in Karaj.

scholarly journals Identification and characterization of antigenic proteins potentially expressed during the infectious process of Paracoccidioides brasiliensis

2009 ◽  
Vol 11 (10-11) ◽  
pp. 895-903 ◽  
Author(s):  
Sabrina Fonseca Ingênito Moreira Dantas ◽  
Tereza Cristina Vieira de Rezende ◽  
Alexandre Melo Bailão ◽  
Carlos Pelleschi Taborda ◽  
Rodrigo da Silva Santos ◽  
...  
Keyword(s):  
Paracoccidioides Brasiliensis ◽  
Infectious Process ◽  
Antigenic Proteins ◽  
Identification And Characterization

First isolation and characterization of Brucella suis from yak

Genome ◽  
2020 ◽  
Vol 63 (8) ◽  
pp. 397-405
Author(s):  
Xiaowen Yang ◽  
Ning Wang ◽  
Xiaofang Cao ◽  
Pengfei Bie ◽  
Zhifeng Xing ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Health And Safety ◽  
Host Cells ◽  
Whole Genome ◽  
Genetic Characteristics ◽  
Qinghai Province ◽  
Isolation And Characterization ◽  
Smooth Strain

Brucella spp., facultative intracellular pathogens that can persistently colonize animal host cells and cause zoonosis, affect public health and safety. A Brucella strain was isolated from yak in Qinghai Province. To detect whether this isolate could cause an outbreak of brucellosis and to reveal its genetic characteristics, several typing and whole-genome sequencing methods were applied to identify its species and genetic characteristics. Phylogenetic analysis based on MLVA and whole-genome sequencing revealed the genetic characteristics of the isolated strain. The results showed that the isolated strain is a B. suis biovar 1 smooth strain, and this isolate was named B. suis QH05. The results of comparative genomics and MLVA showed that B. suis QH05 is not a vaccine strain. Comparison with other B. suis strains isolated from humans and animals indicated that B. suis QH05 may be linked to specific animal and human sources. In conclusion, B. suis QH05 does not belong to the Brucella epidemic species in China, and as the first isolation of B. suis from yak, this strain expands the host range of B. suis.

Real-time characterization of virulence factor expression in Yersinia pestis using a GFP reporter system

2004 ◽  
Vol 324 (2) ◽  
pp. 795-800 ◽  
Author(s):  
Cameron E. Forde ◽  
Jennifer M. Rocco ◽  
J. Patrick Fitch ◽  
Sandra L. McCutchen-Maloney
Keyword(s):  
Yersinia Pestis ◽  
Real Time ◽  
Virulence Factor ◽  
Reporter System ◽  
Gfp Reporter ◽  
Factor Expression
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