Qualitative Indicators of Microbial Cells of Yersinia pestis EV Strain Depending on Their Morphological Traits in Different Temperature Conditions of Manufacturing of Plague Vaccine Preparation

The preference of the (21±1) °C temperature for cultivation of the vaccine strain Yersinia pestis EV in the live plague vaccine biomass production to (27±1) °C is experimentally confirmed. An original interpretation of the mechanism of small cells stability that are formed at (21±1) °C has been proposed. This interpretation stipulates a smaller damaging effect of steam molecules during sublimation in the process of lyophilization.

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Optimisation of the Microbial Cell Concentration Procedure in Plague Vaccine Production

BIOpreparations Prevention Diagnosis Treatment ◽

10.30895/2221-996x-2019-19-1-50-55 ◽

2019 ◽

Vol 19 (1) ◽

pp. 50-55

Author(s):

D. A. Sharov ◽

A. A. Leshchenko ◽

S. V. Bagin ◽

D. A. Mokhov ◽

S. V. Logvinov ◽

...

Keyword(s):

Comparative Study ◽

Yersinia Pestis ◽

Membrane Filtration ◽

Cell Concentration ◽

Microbial Cell ◽

Vaccine Production ◽

Microbial Cells ◽

Native Culture ◽

Physiological Properties ◽

Plague Vaccine

To date, the technology of live plague vaccine production uses a combined method of concentrating microbial cells which consists of three operations with a total duration of 18 hours. The procedure of obtaining concentrate, which is used in the current vaccine production technology, has a number of disadvantages, namely: multiple operations, high energy consumption, long duration, and, as a consequence, low yield of concentrated suspension (0.04 l from 1 l of native culture). The aim of the study was to optimise the procedure of Yersinia pestis ЕV microbial cell concentration using the system for tangential flow microfiltration with the ASF-020 filter support unit. Materials and methods: the vaccine strain used in the study was Yersinia pestis ЕV derived from NIIEG cell line (the strain of the Research Institute of Epidemiology and Hygiene). Submerged cultivation of the native culture was performed using the BIOR-0.25 reactor. The content of live microbial cells was determined by cytorefractometry. Oxidative metabolism was assessed using the chronoamperometric method. Physico-chemical and immunobiological properties of the dry live plague vaccine were assessed according to the monograph FS.3.3.1.0022.15 of the State Pharmacopoeia of the Russian Federation, 14 edition. Results: the equipment’s design features made it possible to carry out membrane filtration of the microbial suspension using the BIOR-0.25 reactor as an intermediate storage unit, thereby excluding three technological stages. The total concentration of microbes in the suspension obtained by the routine and the optimised methods was not less than 120 billion microbial cells/ml. A comparative study of the effect of various hydrodynamic regimes in the working cavities of ASF-009 and ASF020 filter units did not significantly affect the morphometric and physiological properties of microbial cultures. Experimental data helped to determine the process mass balance of membrane filtration. The optimised technology gave 0.17 l yield of the concentrate from 1 l of native culture, and the process duration was reduced to 4 hours. Conclusions: the process of concentrating Y. pestis EV microbial cells during production of plague vaccines was optimised. A comparative study of morphometric and physiological properties of plague microbe cultures that was carried out during their concentration using the optimised technology did not reveal any significant differences as compared to the routine one.

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Assessment of Comparative Genomic Hybridization Experiment by an in situ Synthesized CombiMatrix Microarray with Yersinia pestis Vaccine Strain EV76 DNA

Biomedical and Environmental Sciences ◽

10.1016/s0895-3988(10)60080-3 ◽

2010 ◽

Vol 23 (5) ◽

pp. 384-390 ◽

Cited By ~ 2

Author(s):

Yuan-Hai YOU ◽

Peng WANG ◽

Yan-Hua WANG ◽

Hai-Bin WANG ◽

Dong-Zheng YU ◽

...

Keyword(s):

Yersinia Pestis ◽

Comparative Genomic Hybridization ◽

Vaccine Strain ◽

Hybridization Experiment ◽

Comparative Genomic ◽

Genomic Hybridization ◽

Comparative Genomic Hybridization Experiment

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Construction of a Live-Attenuated Vaccine Strain of Yersinia pestis EV76-B-SHUΔpla and Evaluation of Its Protection Efficacy in a Mouse Model by Aerosolized Intratracheal Inoculation

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2020.00473 ◽

2020 ◽

Vol 10 ◽

Cited By ~ 1

Author(s):

Junxia Feng ◽

Yingying Deng ◽

Mengjiao Fu ◽

Xueyuan Hu ◽

Wenbo Luo ◽

...

Keyword(s):

Mouse Model ◽

Yersinia Pestis ◽

Vaccine Strain ◽

Live Attenuated Vaccine ◽

Attenuated Vaccine ◽

Protection Efficacy

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Yersinia pestis caf1 Variants and the Limits of Plague Vaccine Protection

Infection and Immunity ◽

10.1128/iai.00105-08 ◽

2008 ◽

Vol 76 (5) ◽

pp. 2025-2036 ◽

Cited By ~ 50

Author(s):

Lauriane E. Quenee ◽

Claire A. Cornelius ◽

Nancy A. Ciletti ◽

Derek Elli ◽

Olaf Schneewind

Keyword(s):

Immune Responses ◽

Yersinia Pestis ◽

Protective Immunity ◽

Wild Type ◽

Vaccine Protection ◽

Subunit Vaccines ◽

Humoral Immune ◽

Humoral Immune Responses ◽

Plague Vaccine ◽

Highly Virulent

ABSTRACT Yersinia pestis, the highly virulent agent of plague, is a biological weapon. Strategies that prevent plague have been sought for centuries, and immunization with live, attenuated (nonpigmented) strains or subunit vaccines with F1 (Caf1) antigen is considered effective. We show here that immunization with live, attenuated strains generates plague-protective immunity and humoral immune responses against F1 pilus antigen and LcrV. Y. pestis variants lacking caf1 (F1 pili) are not only fully virulent in animal models of bubonic and pneumonic plague but also break through immune responses generated with live, attenuated strains or F1 subunit vaccines. In contrast, immunization with purified LcrV, a protein at the tip of type III needles, generates protective immunity against the wild-type and the fully virulent caf1 mutant strain, in agreement with the notion that LcrV can elicit vaccine protection against both types of virulent plague strains.

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Determinants of Shoot Biomass Production in Mulberry: Combined Selection with Leaf Morphological and Physiological Traits

Plants ◽

10.3390/plants8050118 ◽

2019 ◽

Vol 8 (5) ◽

pp. 118 ◽

Cited By ~ 3

Author(s):

Xu Cao ◽

Qiudi Shen ◽

Chunqiong Shang ◽

Honglei Yang ◽

Li Liu ◽

...

Keyword(s):

Leaf Area ◽

Total Nitrogen ◽

Biomass Production ◽

Morphological Traits ◽

Nitrogen Concentration ◽

Leaf Expansion ◽

Physiological Traits ◽

Shoot Biomass ◽

Shoot Height ◽

Total Nitrogen Concentration

Physiological and morphological traits have a considerable impact on the biomass production of fast-growing trees. To compare cultivar difference in shoot biomass and investigate its relationships with leaf functional traits in mulberry, agronomic traits and 20 physiological and morphological attributes of 3-year-old mulberry trees from eight cultivars growing in a common garden were analyzed. The cultivars Xiang7920, Yu711, and Yunsang2 had higher shoot fresh biomass (SFB), which was closely associated with their rapid leaf expansion rate, large leaf area, and high stable carbon isotope composition (δ13C). Conversely, the cultivars 7307, Husang32, Wupu, Yunguo1, and Liaolu11 were less productive, and this was primarily the result of slower leaf expansion and smaller leaf size. Growth performance was negatively correlated with leaf δ13C and positively correlated with the total nitrogen concentration, indicating that a compromise exists in mulberry between water use efficiency (WUE) (low δ13C) and high nitrogen consumption for rapid growth. Several morphological traits, including the maximum leaf area (LAmax), leaf width and length, petiole width and length, leaf number per shoot, and final shoot height were correlated with SFB. The physiological traits that were also influential factors of shoot biomass were the leaf δ13C, the total nitrogen concentration, and the water content. Among the studied leaf traits, LAmax, leaf δ13C, and concentrations of chlorophyll a and b were identified as the most representative predictor variables for SFB, accounting for 73% of the variability in SFB. In conclusion, a combination of LAmax, leaf δ13C, and chlorophyll should be considered in selection programs for high-yield mulberry cultivars.

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Yersinia pestis CO92ΔyopH Is a Potent Live, Attenuated Plague Vaccine

Clinical and Vaccine Immunology ◽

10.1128/cvi.00137-07 ◽

2007 ◽

Vol 14 (9) ◽

pp. 1235-1238 ◽

Cited By ~ 30

Author(s):

Sarah S. Bubeck ◽

Peter H. Dube

Keyword(s):

Yersinia Pestis ◽

Live Attenuated Vaccine ◽

Pneumonic Plague ◽

Degree Of Protection ◽

Attenuated Vaccine ◽

Respiratory Challenge ◽

High Degree ◽

Plague Vaccine

ABSTRACT An in-frame deletion of the yopH gene in Yersinia pestis CO92 attenuates virulence in both bubonic and pneumonic plague models. When it is used as a live, attenuated vaccine, CO92ΔyopH provides a high degree of protection from parental and respiratory challenge with Y. pestis CO92.

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