scholarly journals Use of commercial probiotics for the improvement of water quality and rotifer density in outdoor mass culture tanks

2016 ◽  
Vol 63 (4) ◽  
Author(s):  
Jayasree Loka ◽  
S. M. Sonali ◽  
Purbali Saha ◽  
K. Devaraj ◽  
K. K. Philipose
Keyword(s):  
Mass Culture ◽  
Brachionus Plicatilis ◽  
Nitrifying Bacteria ◽  
Control Group ◽  
Probiotic Treatment ◽  
Rotifer Brachionus Plicatilis ◽  
Bacillus Spp ◽  
Outdoor Mass Culture ◽  
Commercial Probiotics ◽  
Total Elimination

An experimental study was carried out to evaluate the efficacy of three commercially available probiotics P1, P2 and P3 consisting of mainly Bacillus spp. and nitrifying bacteria against Vibrio loads in mass culture tanks of the rotifer Brachionus plicatilis. Triplicate tanks were maintained for each of the probiotic treatment as well as for control group. All the tanks were inoculated with 50 rotifers ml-1 and were fed with Nannochloropsis oculata at a density of 1 x 107 cells ml-1. Every alternate day, all the experimental tanks were treated with probiotics at a concentration of 1 x 104 cfu ml-1 and the experiment was carried out for one week. The study showed a significant increase in rotifer density (p<0.05) in all the tanks treated with the probiotics and a maximum density of 400 nos. ml-1 was observed in the tanks treated with P3. After 5th and 6th day of culture, total elimination of Vibrios was also recorded in the tanks treated with P3 and P2 respectively. The study revealed that P3, with a combination of Bacillus, Thiobacillus, Acetobacter and Paracoccus supplemented with enzymes, was found to be most effective in the enhancement of rotifer density and also in the elimination of Vibrios in rotifer mass culture tanks.

scholarly journals Probiotics Modulate Tilapia Resistance and Immune Response against Tilapia Lake Virus Infection

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 919
Author(s):  
Pitchaporn Waiyamitra ◽  
Mehmet Arif Zoral ◽  
Aksorn Saengtienchai ◽  
Amorn Luengnaruemitchai ◽  
Olivier Decamp ◽  
...  
Keyword(s):  
Immune Response ◽  
Viral Load ◽  
Control Diet ◽  
Expression Patterns ◽  
Economic Damage ◽  
Control Group ◽  
Probiotic Treatment ◽  
Significant Difference ◽  
Bacillus Spp ◽  
Immune Related Genes

Tilapia lake virus (TiLV) causes an emerging viral disease associated with high mortality and economic damage in tilapia farming around the world. The use of probiotics in aquaculture has been suggested as an alternative to antibiotics and drugs to reduce the negative impact of bacterial and viral infections. In this study, we investigate the effect of probiotic Bacillus spp. supplementation on mortality, viral load, and expression of immune-related genes in red hybrid tilapia (Oreochromis spp.) upon TiLV infection. Fish were divided into three groups, and fed with: control diet, 0.5% probiotics-supplemented diet, and 1% probiotics-supplemented diet. After 21 days of experimental feeding, the three groups were infected with TiLV and monitored for mortality and growth performances, while organs were sampled at different time points to measure viral load and the transcription modulation of immune response markers. No significant difference was found among the groups in terms of weight gain (WG), average daily gain (ADG), feed efficiency (FE), or feed conversion ratio (FCR). A lower cumulative mortality was retrieved from fish fed 0.5% and 1% probiotics (25% and 24%, respectively), compared to the control group (32%). Moreover, fish fed with 1% probiotic diet had a significantly lower viral load, than those fed with 0.5% probiotic and control diet at 5, 6, 9, and 12 days post infection-challenge (dpc). The expression patterns of immune-related genes, including il-8 (also known as CXCL8), ifn-γ, irf-3, mx, rsad-2 (also known as VIPERIN) showed significant upregulation upon probiotic treatment during the peak of TiLV pathogenesis (between 9 and 12 dpc) and during most of the study period in fish fed with 1% probiotics-supplemented diet. Taken together, these findings indicate that dietary supplementation using Bacillus spp. probiotics may have beneficial effects to strengthen tilapia immunity and resistance against TiLV infections. Therefore, probiotic treatments may be preventively administered to reduce losses caused by this emerging viral infection in tilapia aquaculture.

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