scholarly journals Prevalence of vibrios with special reference to Vibrio parahaemolyticus in farmed penaeid shrimp, Penaeus vannamei (Boone, 1931) from selected districts of Tamil Nadu, India

2017 ◽  
Vol 64 (3) ◽  
Author(s):  
R. Ananda Raja ◽  
R. Sridhar ◽  
C. Balachandran ◽  
A. Palanisamy ◽  
S. Ramesh ◽  
...  
Keyword(s):  
Special Reference ◽  
Vibrio Parahaemolyticus ◽  
Tamil Nadu ◽  
Penaeid Shrimp ◽  
Penaeus Vannamei ◽  
Isolation And Identification ◽  
Biochemical Characterisation ◽  
Identification Of Bacteria ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Physiological And Biochemical

Prevalence of Vibrio spp. with special reference to Vibrio parahaemolyticus (Vp) infection was assessed in Penaeus vannamei farms in Kancheepuram and Thiruvallur districts of Tamil Nadu during the period August 2014 to February 2015. Isolation and identification of bacteria from haemolymph, stomach and hepatopancreas of P. vannamei was done from all the farms for the prevalence of Vp infection. Based on the morphological, physiological and biochemical characterisation, 74 isolates were identified as Vp (35.14%), V. harveyi (21.62%), V. anguillarum (16.22%), V. campbellii (10.81%), V. mimicus (8.11%), V. alginolyticus (5.41%) and Pseudomonas aeruginosa (2.7%). The PCR results showed that there were 26 (35.14%) isolates positive for Vp specific toxR and tlh genes and negative for human pathogenic tdh and trh genes. All isolates were also negative for acute hepatopancreatic necrosis disease (AHPND) specific AP1, AP2, AP3 (pirAvp) and AP4 (pirAvp and pirBvp) genes. The study revealed that there was no AHPND causing Vp strain in farmed P. vannamei from two important coastal districts of Tamil Nadu, India.

scholarly journals Effects of Feed Mixed with Lactic Acid Bacteria and Carbon, Nitrogen, Phosphorus Supplied to the Water on the Growth and Survival Rate of White Leg Shrimp (Penaeus vannamei) Infected with Acute Hepatopancreatic Necrosis Disease Caused by Vibrio parahaemolyticus

Biology ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 280
Author(s):  
Linh Nguyen Thi Truc ◽  
Tuu Nguyen Thanh ◽  
To Tran Thi Hong ◽  
Day Pham Van ◽  
Minh Vo Thi Tuyet ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Survival Rate ◽  
Lactic Acid Bacteria ◽  
Vibrio Parahaemolyticus ◽  
Control Group ◽  
Penaeus Vannamei ◽  
Growth And Survival ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Nitrogen Phosphorus ◽  
Shrimp Health

This study aimed to evaluate the growth, survival rate, and resistance to acute hepatopancreatic necrosis disease (AHPND) of white leg shrimp (Penaeus vannamei) by using Lactobacillus plantarum, Lactobacillus fermentum, and Pediococcus pentosaceus mixed with feed, and at the same time supplying CNP in a ratio of 15:1:0.1 to the water. As a result, the treatments that shrimp were fed with feed containing lactic acid bacteria (LAB), especially L. plantarum, have increased shrimp growth, total hemocyte cells, granulocyte cells, and hyaline cells significantly (p < 0.05) in comparison to the control group. The supply of CNP to the water has promoted the intensity of V. parahaemolyticus effects on shrimp health and significantly decreased total hemocyte cells, granulocyte cells, and hyaline cells by 30–50% in the period after three days of the challenge, except in L. plantarum treatment, which had only a 20% decrease compared to other treatments. In CNP supplying treatments, the AHPND infected rate and mortality of shrimp were higher than those in other treatments. In summary, the supply of CNP had significantly reduced the shrimp’s immune response and promoted the susceptibility of shrimp to AHPND in both cases of use with and without LAB-containing diets.

scholarly journals Draft Genome Sequence of the Shrimp Pathogen Vibrio parahaemolyticus ST17.P5-S1, Isolated in Peninsular Malaysia

2018 ◽  
Vol 7 (11) ◽  
Author(s):  
Sridevi Devadas ◽  
Subha Bhassu ◽  
Tze Chiew Christie Soo ◽  
Fatimah M. Yusoff ◽  
Mohamed Shariff
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Vibrio Parahaemolyticus ◽  
East Coast ◽  
Draft Genome ◽  
Antibiotic Resistance Genes ◽  
Peninsular Malaysia ◽  
Penaeus Vannamei ◽  
Content Type ◽  
Acute Hepatopancreatic Necrosis Disease

We sequenced the genome of Vibrio parahaemolyticus strain ST17.P5-S1, isolated from Penaeus vannamei cultured in the east coast of Peninsular Malaysia. The strain contains several antibiotic resistance genes and a plasmid encoding the Photorhabdus insect-related (Pir) toxin-like genes, pirAvp and pirBvp, associated with acute hepatopancreatic necrosis disease (AHPND).

Metabolic responses of penaeid shrimp to acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus

Aquaculture ◽  
2021 ◽  
Vol 533 ◽  
pp. 736174
Author(s):  
Thao V. Nguyen ◽  
Andrea Alfaro ◽  
Bonny Bayot Arroyo ◽  
Jenny Antonia Rodriguez Leon ◽  
Stanislaus Sonnenholzner
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Penaeid Shrimp ◽  
Metabolic Responses ◽  
Acute Hepatopancreatic Necrosis Disease

scholarly journals Complete Genome Sequence of a Deletion Mutant of Vibrio parahaemolyticus from Pacific White Shrimp (Penaeus vannamei)

2018 ◽  
Vol 6 (25) ◽  
Author(s):  
Siddhartha Kanrar ◽  
Arun K. Dhar
Keyword(s):  
Genome Sequence ◽  
Vibrio Parahaemolyticus ◽  
Deletion Mutant ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Penaeus Vannamei ◽  
Content Type ◽  
A Genome ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Experimental Challenge

ABSTRACT Vibrio parahaemolyticus carrying the toxin genes pirA and pirB causes acute hepatopancreatic necrosis disease in shrimp. A genome sequence of V. parahaemolyticus strain R13 was determined that showed deletions of the entire pirA gene and the 5ʹ end of the pirB gene and does not cause the disease in experimental challenge.

scholarly journals The opportunistic marine pathogenVibrio parahaemolyticusbecomes virulent by acquiring a plasmid that expresses a deadly toxin

2015 ◽  
Vol 112 (34) ◽  
pp. 10798-10803 ◽  
Author(s):  
Chung-Te Lee ◽  
I-Tung Chen ◽  
Yi-Ting Yang ◽  
Tzu-Ping Ko ◽  
Yun-Tzu Huang ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Penaeid Shrimp ◽  
Structural Similarity ◽  
Gene Organization ◽  
Structural Topology ◽  
Cry Protein ◽  
Insecticidal Toxin ◽  
Shrimp Industry ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Postsegregational Killing

Acute hepatopancreatic necrosis disease (AHPND) is a severe, newly emergent penaeid shrimp disease caused byVibrio parahaemolyticusthat has already led to tremendous losses in the cultured shrimp industry. Until now, its disease-causing mechanism has remained unclear. Here we show that an AHPND-causing strain ofV. parahaemolyticuscontains a 70-kbp plasmid (pVA1) with a postsegregational killing system, and that the ability to cause disease is abolished by the natural absence or experimental deletion of the plasmid-encoded homologs of thePhotorhabdusinsect-related (Pir) toxins PirA and PirB. We determined the crystal structure of theV. parahaemolyticusPirA and PirB (PirAvpand PirBvp) proteins and found that the overall structural topology of PirAvp/PirBvpis very similar to that of theBacillusCry insecticidal toxin-like proteins, despite the low sequence identity (<10%). This structural similarity suggests that the putative PirABvpheterodimer might emulate the functional domains of the Cry protein, and in particular its pore-forming activity. The gene organization of pVA1 further suggested thatpirABvpmay be lost or acquired by horizontal gene transfer via transposition or homologous recombination.

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