Isolation of β-caroten from Brown Tiger Shrimp shell waste (Penaeus esculentus) and its antioxidant activity using ABTS (2,2-azinobis- [3-ethylbenzothiazoline-6-sulfonicacid] method

BrownTiger Shrimp (Penaeus esculentus) is a fishery export commodity that has high potential and categorize major commercial value.The waste in the form of shell produced from the shrimp industry has not been used properly and efficiently. In fact, most of it is waste that also pollutes the environment.Shrimp shell contained a lot of carotenoid pigments which are antioxidants, one of which is β-carotene.The aim of the study was to determine the amount of β-carotene and antioxidant activity in the chloroform extract of BrownTiger shrimp shell (Penaeus esculentus). The study used maceration method with chloroform to obtain β-carotene extract then the total content was measured using UV-Vis spectrophotometry.Testing the antioxidant activity of β-carotene and chloroform β-carotene extract of Brown Tiger shrimp shell (Penaeus esculentus) was carried out through free radical scavenging using the ABTS method (2,2-azinobis- [3-ethylbenzothiazoline-6-sulfonicacid]).Chloroform β-carotene extract of Brown Tiger shrimp shell (Penaeus esculentus) contained β-carotene 618.2 µg / g of extract (0.06182% per gram of extract), and had weak antioxidant activity with IC50 value of 396.660 mg/L.β-carotene as a comparison compound has an IC50 value of 114.838 with moderate antioxidant activity category. The conclusion of this study was the chloroform β-carotene extract of Brown Tiger shrimp skin (Penaeus esculentus) contained β-carotene compounds and had weak antioxidant activity index.Keywords: β-carotene, shrimp shell, Penaeus esculentus, antioxidant, ABTSABSTRAKIsolasi β–karoten limbah kulit udang tiger coklat (Penaeus Esculentus) dan aktivitas antioksidannya dengan metode ABTS(2,2-Azinobis- [3-Ethylbenzothiazoline-6-Sulfonicacid])Udang Tigercoklat (Penaeus esculentus) merupakan komoditas ekspor perikanan yang memiliki potensi tinggi dan dikategorikan sebagai udang yang memiliki nilai komersial utama. Limbah berupa kulit yang dihasilkan dari industri udang belum dimanfaatkan dengan baik dan efisien, bahkan sebagian besar merupakan limbah yang mencemari lingkungan. Kulit udang banyak mengandung pigmen karotenoid yang merupakan antioksidan, salah satunya adalah β-karoten. Tujuan dari penelitian ini adalah untuk mengetahui kadarβ-karoten dan aktivitas antioksidan ekstrak kloroform kulit udang Tigercoklat dengan menggunakan β-karoten sebagai pembanding. Penelitian ini menggunakan metode ekstraksi maserasi dengan kloroform untuk mendapatkan ekstrak kloroformβ-karoten dan mengukur kadarnya menggunakan spektrofotometri UV-Vis. Pengujian aktivitas antioksidan β-karoten dan ekstrak kloroformβ-karoten kulit udang Tigercoklat dilakukan melalui pemulungan radikal bebas menggunakan metode ABTS (2,2-azinobis-[3-ethylbenzothiazoline-6-sulfonicacid]). Ekstrak kloroform β-karoten kulit udang Tigercoklat mengandung β-karoten sebanyak 618,2 µg / g ekstrak (0,06182% per gram ekstrak), dan memiliki aktivitas antioksidan lemah dengan nilai IC50 396,660 mg/L. β-karoten sebagai senyawa pembanding memiliki nilai IC50 sebesar 114,838 ppm dengan kategori sedang. Kesimpulan dari penelitian ini adalah ekstrak kloroform β-karotenkulit udang Tigercoklat mengandung senyawa β-karoten dan memiliki aktivitas antioksidan dalam kategori lemah.Kata kunci: β-karoten; kulit udang;, Penaeus esculentus; antioksidan; ABTS

Effects of Microencapsulated Organic Acid and Their Salts on Growth Performance, Immunity, and Disease Resistance of Pacific White Shrimp Litopenaeus vannamei

Use of antibiotics and other chemicals to combat disease outbreaks has been a bottleneck for the sustainable growth of shrimp industry. Among various replacements proposed, organic acid (OA) and their salts (OS) are commonly used by farmers and feed millers. However, in free forms, their requirement is very high (2–3 kg/MT) as they tend to disassociate before reaching the hindgut. The dosage can be reduced by microencapsulation of the ingredients. In this study, a 63-day trial was conducted to assess the effects of OA and OS (COMP) microencapsulated (ENCAP) with fat (HF), fat + alginate (HA), wax esters (WE) and HA + WE (HAWE) on performance, digestive enzymes, immunity and resistance to Vibrio parahaemolyticus. A positive control (PC, 200 g/kg fishmeal-FM) and a negative control (NC, 130 g/kg FM) diet were formulated. Eight other diets were formulated, supplementing an NC diet with microencapsulated OA (OAHF, OAHA, OAWE, OAHAWE) and OS (OSHF, OSHA, OSWE, OSHAWE). Among the ENCAPs, significant difference was observed in serum malondialdehyde (p = 0.026), where HF showed the lowest level (6.4 ± 0.3 mmol/L). Significant interactions between COMP and ENCAP were observed in lipid deposition (p = 0.047), serum alkaline phosphatase, acid phosphatase, hepatopancreatic and serum phenol oxidase (p < 0.0001). Despite no differences, 96-h mortality during pathogenic Vibrio parahaemolyticus challenge in all treatment diets (45–56%) was lower compared to the NC diets (63%). In conclusion, use of HF microencapsulated OA diets could provide improved performance and disease resistance that could contribute to the reduction of antibiotic use by the shrimp industry.

Effects of Microencapsulated Organic Acid and Their Salts on Growth Performance, Immunity, and Disease Resistance of Pacific White Shrimp Litopenaeus vannamei

Use of antibiotics and other chemicals to combat disease outbreaks have been a bottleneck for the sustainable growth of shrimp industry. Among various replacement proposed, organic acid (OA) and their salts (OS) are commonly used by farmers and feed millers. However, in free forms, their requirement is very high (2-3 kg/MT) as they tend to disassociate before reaching the hindgut. The dosage can be reduced by microencapsulation of the ingredients. In this study, a 63-day trial was conducted to assess the effects of OA and OS (COMP) microencapsulated (ENCAP) with fat (HF), fat + alginate (HA), wax esters &ndash; (WE), and HA and WE (HAWE) on performance, digestive enzyme, immune, and resistance to Vibrio parahaemolyticus. A positive control (PC, 200 g/kg fishmeal - FM) and a negative control (NC, 130 g/kg FM) diet were formulated. Eight other diets were formulated supplementing NC diet with microencapsulated OA (OAHF, OAHA, OAWE, OAHAWE) and OS (OSHF, OSHA, OSWE, OSHAWE). Among the ENCAPs, significant difference was observed in serum malondialdehyde (P = 0.026) where HF showed the lowest level (6.4 &plusmn;0.3 mmol/L). Significant interactions between COMP and ENCAP were observed in lipid deposition (P = 0.047), serum alkaline phosphatase and acid phosphatase (P &lt; 0.0001), and hepatopancreatic and serum phenol oxidase (P &lt; 0.0001). Despite no differences, 96-h mortality during pathogenic Vibrio parahaemolyticus challenge in all treatment diets (45% - 56%) was lower compared to the NC diets (63%). In conclusion, use of HF microencapsulated OA diets could provide improved performance and disease resistance that could contribute to the reduction of antibiotic use by the shrimp industry.

Current status of acute hepatopancreatic necrosis disease (AHPND) and result of isolating AHPND-causing strains of farmed shrimp in Mekong Delta Region of Vietnam

Early mortality syndrome/acute hepatopancreatic necrosis (EMS/AHPND) was first detected in China in 2009. The disease spread rapidly to neighboring countries and emerged in almost major shrimp-producing regions in the world, including Vietnam. The disease has caused serious damage to the global shrimp industry and so far, there is no effective cure. In order to understand the current status of AHPND, and then to introduce effective prevention and detection measures, we collected data and shrimp samples in some provinces in the Mekong Delta to analyze and isolate the pathogenic strains. The results of our study conducted from 2014 - 2018 in four provinces (Ben Tre, Long An, Bac Lieu, Kien Giang) showed that AHPND damaged from 2.0 to 57.2% of the total shrimp farming area. In addition, we isolated 10 AHPND-positive strains via culturing and PCR. The results of representative sequencing of three strains LA1, LA5, and LA8 showed that they were 100% similarity with the previously published strain XN89. These isolated strains are used as a collection for further studies on the origin and mechanism of the disease by whole genome sequencing.

De campesinos temporaleros a obreros de congeladoras de camarón en Campeche, México

En el marco del proceso histórico de surgimiento de la industria camaronera en un poblado rural de Campeche, a mediados del siglo XX, el objetivo de este artículo es explorar la transformación de sus campesinos de subsistencia en obreros de las congeladoras de mariscos. Para ello, se concentra en la constitución de varios espacios sociales emergentes entrelazados, cuyos procesos de organización de sus relaciones han enfatizado la producción de jerarquías y desigualdades entre los grupos: la organización transnacional de la industria camaronera, las congeladoras, el sindicato y la participación política corporativa. En esos espacios, los campesinos se fueron constituyendo y siendo constituidos como obreros explotados, sindicalizados dóciles y masas políticas corporativamente disciplinadas. Sus condiciones de desigualdad fueron resultado de procesos de producción y ordenamiento asimétrico de espacios e instituciones promovidos por la formación conjunta del Estado y el capitalismo en la zona.Palabras clave: Estado, Capital, Desigualdades de clase, Relaciones de poder, Orden social From seasonal farmers to workers in the shrimp freezers in Campeche, MexicoSummaryWithin the framework of the historical process of the emergence of the shrimp industry in a rural town of Campeche, in the middle of the 20th century, the objective of this article is to explore the transformation of its subsistence farmers into workers of the seafood freezers. To do this, it focuses on the constitution of several intertwined emerging social spaces, whose processes of organization of their relationships have emphasized the production of hierarchies and inequalities between groups: the transnational organization of the shrimp industry, freezers, the union and corporate political participation. In these spaces, the peasants constituted and were constituted as exploited workers, docile union members and corporately disciplined political masses. Their conditions of inequality were the result of processes of production and asymmetric arrangement of spaces and institutions promoted by the joint formation of the State and capitalism in the area.Keywords: State, Capital, Class inequalities, Power relations, Social order De paysans temporaires à ouvriers de congélateurs de crevette à Campeche, MexiqueRésuméDans le cadre du processus historique du début de l’industrie crevettière dans le village rural de Campeche vers la moitié du XXe. siècle, l’objectif de cet article est celui d’explorer la transformation de ses paysans de subsistance en ouvriers des congélateurs de crevettes. Pour cela, on se centre dans la constitution de quelques espaces sociaux émergents entrelacés, dont les processus d’organisation de leurs relations ont mis l’accent sur la production d’hiérarchies et d’inégalités parmi les groupes : l’organisation transnationale de l’industrie crevettière, les congélateurs, le syndicat et la participation politique corporative. Dans ces espaces, les paysans se sont constitués et ainsi sont devenus des ouvriers exploités, syndicalisés dociles et masses politiques disciplinées au niveau corporatif. Leurs conditions d’inégalité ont été le résultat de processus de production et rangement asymétrique d’espaces et institutions promus par la formation commune de l’État et le capitalisme de la zone.Mots clés : État, Capital, Inégalités de classe, Relations de pouvoir, Ordre social

Multi-Criteria Decision-Making Methods in Fuzzy Decision Problems: A Case Study in the Frozen Shrimp Industry

The European Union (EU) is the largest shrimp consumer market in the world in terms of requirements for shrimp product imports. Therefore, other enterprises that export frozen shrimp to the EU must consider many criteria when choosing suppliers of raw shrimp. The difficulty of choosing suppliers of raw shrimp makes selecting raw material suppliers in the fisheries sector a multi-criteria decision-making problem. In such problems, the decision makers must review and evaluate many criteria—including qualitative and quantitative factors—to achieve an optimal result. While there have been multiple multi-criteria decision making models developed to support supplier selection processes in different industries, none of these have been developed to solve the particular problems facing the shrimp industry, especially as it concerns a fuzzy decision-making environment. In this research, the authors propose a Multi-Criteria Decision Making model (MCDM) including the Fuzzy Analytical Network Process (FANP) and Weighted Aggregated Sum Product Assessment (WASPAS) for the evaluation and selection process of shrimp suppliers in the fisheries industry. The model is applied to a real-world case study and the results show that Supplier 3 (SA3) is the most optimal supplier of raw shrimp. The contribution of this work is the employment of FANP and WASPAS to propose an MCDM for ranking potential suppliers in the fisheries industry in a fuzzy environment. The proposed approach can also be modified to support complex decision-making processes in fuzzy environments in different industries.

Inherent Safety Analysis and Sustainability Evaluation of Chitosan Production from Shrimp Exoskeleton in Colombia

Waste valorization strategies are key to achieve more sustainable production within the shrimp industry. The crustacean exoskeletons can be potentially used to obtain value-added products such as chitosan. A comprehensive analysis including both safety and sustainability aspects of chitosan production from shrimp shells is presented in this study. The inherent safety analysis and sustainability evaluation was performed using the Inherent Safety Index (ISI) methodology and the Sustainable Weighted Return on Investment Metric (SWROIM), respectively. The process was designed for a processing capacity of 57,000 t/year. The return on investment (%ROI), potential environmental impact (PEI output), exergy efficiency, and the total inherent safety index (ITI) were used as indicators to evaluate process sustainability. The total inherent safety index was estimated at 25 indicating that the process is inherently unsafe. The main process risks were given by handling of flammable substances, reactivity, and inventory subindices. The overall sustainability evaluation showed a SWROIM of 36.33% indicating that the case study showed higher weighted performance compared to the return on investment metric of 18.08%.