Characterization, mRNA expression and nutritional regulation of a Δ6 fatty acyl desaturase-like gene by dietary DHA levels of Perinereis aibuhitensis (Gube, 1878)

The Δ6 fatty acyl desaturase (Δ6 Fad) enzyme plays an important role in the pathways for the biosynthesis of the highly unsaturated fatty acids (HUFA) by converting linoleic (LA, 18:2n-6) and α-linolenic (LNA, 18:3n-3) acids to 18:3n-6 and 18:4n-3, respectively. In the present study, a Δ6 Fad-like gene was cloned and its characterisation, tissue expression and nutritional regulation by dietary DHA (docosahexaenoic acid) levels were analysed in the clamworm Perinereis aibuhitensis. The full-length cDNA was 1535 bp including a 1335 bp open reading frame (ORF) encoding a polypeptide of 444 amino acids, a 113 bp 50’-UTR and an 87 bp 3’-UTR with a poly (A) tail. Sequence comparison revealed that the predicted protein shared higher homologies with Δ6 Fad from Apostichopus japonicus with 56% identify. The tissue distribution of P. aibuhitensis Δ6 Fad-like mRNA was found predominantly in intestine, followed by head, esophagus, stomach and other tissues. Quantitative real-time PCR showed that the Δ6 Fad-like transcriptional levels in intestine gradually decreased with increased content of dietary DHA.

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The capacity for long-chain polyunsaturated fatty acid synthesis in a carnivorous vertebrate: Functional characterisation and nutritional regulation of a Fads2 fatty acyl desaturase with Δ4 activity and an Elovl5 elongase in striped snakehead (Channa striata)

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids ◽

10.1016/j.bbalip.2014.12.012 ◽

2015 ◽

Vol 1851 (3) ◽

pp. 248-260 ◽

Cited By ~ 52

Author(s):

Meng-Kiat Kuah ◽

Annette Jaya-Ram ◽

Alexander Chong Shu-Chien

Keyword(s):

Fatty Acid ◽

Fatty Acid Synthesis ◽

Acid Synthesis ◽

Fatty Acyl ◽

Chain Polyunsaturated Fatty Acid ◽

Nutritional Regulation ◽

Functional Characterisation ◽

Fatty Acyl Desaturase ◽

Channa Striata ◽

Long Chain

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Long-chain polyunsaturated fatty acid biosynthesis in the euryhaline herbivorous teleost Scatophagus argus: Functional characterization, tissue expression and nutritional regulation of two fatty acyl elongases

Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology ◽

10.1016/j.cbpb.2016.03.009 ◽

2016 ◽

Vol 198 ◽

pp. 37-45 ◽

Cited By ~ 27

Author(s):

Dizhi Xie ◽

Fang Chen ◽

Siyuan Lin ◽

Cuihong You ◽

Shuqi Wang ◽

...

Keyword(s):

Fatty Acid ◽

Polyunsaturated Fatty Acid ◽

Fatty Acid Biosynthesis ◽

Functional Characterization ◽

Tissue Expression ◽

Fatty Acyl ◽

Chain Polyunsaturated Fatty Acid ◽

Nutritional Regulation ◽

Acid Biosynthesis ◽

Long Chain

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FATTY ACID COMPOSITION OF PORCINE DEPOT FAT AS RELATED TO THE EFFECT OF SUPPLEMENTAL DIETARY COPPER ON THE SPECIFIC ACTIVITIES OF FATTY ACYL DESATURASE SYSTEMS

Canadian Journal of Animal Science ◽

10.4141/cjas74-004 ◽

1974 ◽

Vol 54 (1) ◽

pp. 23-28 ◽

Cited By ~ 16

Author(s):

S. K. HO ◽

J. I. ELLIOT

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Unsaturated Fatty Acids ◽

Average Daily Gain ◽

Fatty Acyl ◽

Dietary Copper ◽

Liver Copper ◽

Fatty Acyl Desaturase ◽

Specific Activities

Copper supplementation of a swine diet (barley-wheat-soybean meal) at a level of 250 ppm was associated with improved average daily gain, reduction in the melting point of the depot fat, increase in liver copper, decreased proportions of stearic and palmitic acids and concomitant increases in proportion of the medium-chain myristic acid as well as those of the major long-chain unsaturated fatty acids in the depot fat, and increases in the capacities of hepatic and adipose microsomes to desaturate 1-14C-oleate (in the presence of coenzyme A, ATP, Mg2+) and 1-14C-palmitoyl-CoA. There were neither sex differences nor copper × sex interactions. It is suggested that supplemental dietary copper enhances the specific activities of the fatty acyl desaturase systems, and that this enhancement contributes to the observed changes in fatty acid composition of the depot fat.

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