scholarly journals Peranan Zat Pengatur Tumbuh dalam Perbanyakan Tanaman melalui Kultur Jaringan

2011 ◽  
Vol 7 (1) ◽  
pp. 63 ◽  
Author(s):  
Endang Gati Lestari
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Plant Growth ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Plant Tissue ◽  
Callus Production ◽  
Control Root ◽  
Other Substances

<p>The Role of Growth Regulator in Tissue Culture Plant<br />Propagation. Endang G. Lestari. In plant tissue culture,<br />growth regulator has significant roles such as to control root<br />and shoot development in the plant formation and callus<br />induction. Cytokinin and auxin are two prominent growth<br />regulator. Cytokinin consists of BA (benzil adenin), kinetin<br />(furfuril amino purin), 2-Ip (dimethyl allyl amino purin), and<br />zeatin. While auksin covers IAA (indone acetic acid), NAA<br />(napthalene acetic acid), IBA (indole butiric acid) 2.4-D (2.4-<br />dicholophenoxy acetic acid), dicamba (3,6 dicloro-O-anisic<br />acid), and picloram (4-amino 3,5,6-tricloropicolinic acid).<br />The emphasis of plant growth purposes decide the use of<br />growth regulator. Cytokinin is applied mainly for the purpose<br />of shoot, while auxin is mainly used for the purpose of root<br />and callus. The application of growth regulator application is<br />varied, depending on the genotype and physiological<br />condition of the plant. The existence of a certain growth<br />regulating substances can enhance growth regulator activity<br />of other substances. The type and concentration of the<br />appropriate growth regulators for each plant is not the same<br />because it depends on the genotype and physiological<br />condition of plant tissue. However so often both are<br />frequently required depend on the ratio/ratio of auxin<br />cytokines or vice versa. The existence of a certain growth<br />regulating substances can enhance growth regulator activity<br />of other substances. The type and concentration of the<br />appropriate growth regulators for each plant is not the same<br />because it depends on the genotype and physiological<br />condition of plant tissue. For the propagation, multiple and<br />adventive shoots along with embriosomatic formation could<br />be applied. The seedling is obtained from one somatic cell.<br />Here, strong auxin, such as dicamba and picloram 2.4-D, is<br />utilized for callus production. For this reason, seedling per<br />unit could be produced more than that of organogenesis.</p>

scholarly journals Maximizing Performance of Plant Growth Regulators by Improving Spray Application

2005 ◽  
Vol 15 (2) ◽  
pp. 222-231 ◽  
Author(s):  
Martin J. Bukovac
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Active Ingredient ◽  
Plant Tissue ◽  
Spray Application ◽  
Application Process ◽  
Component Processes ◽  
The Individual

The importance of spray application and the role of spray additives are reviewed in reference to increasing the effectiveness of plant growth regulators (PGR). The spray application process is composed of a number of interrelated components, from formulation of the active ingredient into a sprayable, bioactive solution (emulsion/suspension), to atomization, delivery, retention, and penetration into the plant tissue. Each of these events is critical to performance of the PGR. Also, each can be affected by spray additives, particularly adjuvants, which may be incorporated in the formulation of the active ingredient or added to the spray mixture. The role of the individual components and effects of spray adjuvants, particularly surfactants and fertilizer adjuvants, on the component processes are discussed.

Plant hormones and plant growth regulators in plant tissue culture

1996 ◽  
Vol 32 (4) ◽  
pp. 272-289 ◽  
Author(s):  
Thomas Gaspar ◽  
Claire Kevers ◽  
Claude Penel ◽  
Hubert Greppin ◽  
David M. Reid ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Plant Hormones ◽  
Plant Tissue Culture ◽  
Plant Tissue

scholarly journals Effect of adding growth regulators benzyl adenine (BA) and kinetin (Kin) on production of potato (spunta) micro tuber

2019 ◽  
pp. 1-12
Author(s):  
Salem Hammud ◽  
Ahmed shaaban ◽  
Elmundr Abughnia ◽  
Kheiry Keer ◽  
Arij shaheen
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Potato Tuber ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Potato Variety ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Tuber Formation ◽  
Average Weight

This experiment was carried out in plant tissue culture laboratory a part of bio technology research center (BTRC) , while the main aim for this experiment was to investigate the effects of using plant growth regulators on potato micro tuber formation for Sponta potato variety throw plant tissue culture method , In the beginning micro propagation operations were done to the target potato variety in order to obtain enough number of plants samples in free contamination MS media , while different concentrations of BA and Kin plant growth regulators ( 0 , 1 , 2 , 3 mg/l ) were used in this experiment , the plant growth regulators BA ,Kin were added to MS media contain 60g/l sucrose during micro tuber formation stage . the results of this study showed that the treatment of (5mg/l Kin ) obtained the best results and this treatment gave the highest tuber production compared with other treatments , furthermore this treatment ( 5mg/l Kin) gave the highest number of produced potato tuber and the highest weight of produced tuber compared with other used treatments , while the average number of produced micro tubers arrived to 4.9 tuber for each plant and the average weight for obtained potato tuber arrived to 0.84 g which was higher than other weight of micro tuber produced from the other treatments .

scholarly journals Hyaluronic Acid Can be a New Plant Growth Regulator for Hybrid Cymbidium Micropropagation

2012 ◽  
Vol 22 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Naruemol Kaewjampa ◽  
Kazuhiko Shimasaki ◽  
Syeda Jabun Nahar
Keyword(s):  
Tissue Culture ◽  
Hyaluronic Acid ◽  
Plant Growth ◽  
Growth Regulator ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Plant Growth Regulator ◽  
Culture Medium ◽  
Fresh Weight ◽  
Maximum Percentage

Present authors investigated the effect of two biopolymer hyaluronic acid (HA9) and chitosan (Chitosan H), and N6-benzyladenine (BA) supplemented with modified MS on organogenesis in protocorm-like bodies (PLBs) of a Cymbidium cultivar. The percentages of PLB formation from cultures showed 100 % in all treatments except for control (82.7%), 0.1 mg/l HA9 (93.3%) and 10 mg/l Chitosan H (93.3%) treatments. Addition of 0.1 mg/l HA9 to culture medium was highly efficient for PLB multiplication. Both 10 mg/l HA9 and 1.0 mg/l BA significantly increased the number of shoots in cultures. The maximum percentage of shoot (100%) was obtained at 1.0 mg/l chitosan H. Increase in fresh weight showed higher values at BA treatments. Information gained from this study shows that hyaluronic acid (HA9) which is new additive for plant tissue culture act as growth regulator for hybrid Cymbidium micropropagation. DOI: http://dx.doi.org/10.3329/ptcb.v22i1.11261Plant Tissue Cult. & Biotech. 22(1): 59-64, 2012 (June)

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