scholarly journals EVALUASI KERAGAMAN GENETIK PLASMA NUTFAH KELAPA GENJAH DI KEBUN PERCOBAAN MAPANGET BERDASARKAN PENANDA DNA SSRs (Simple Sequence Repeats)

2020 ◽  
Vol 12 (3) ◽  
pp. 116
Author(s):  
JEANETTE KUMAUNANG ◽  
ISMAIL MASKROMO ◽  
ENGELBERT MANAROINSONG
Keyword(s):  
Genetic Diversity ◽  
Key Words ◽  
Simple Sequence Repeats ◽  
Genetic Similarity ◽  
Cocos Nucifera ◽  
Ex Situ ◽  
Cocos Nucifera L ◽  
Simple Sequence ◽  
Diversity Evaluation ◽  
Experimental Garden

ABSTRAK<br />Keragaman genetik sangat penting dalam program pemuliaan<br />kelapa. Penelitian ini bertujuan untuk mempelajari keragaman genetik<br />plasma nutfah kelapa Genjah yang ditanam secara ex situ di kebun koleksi<br />Mapanget, Balai Penelitian Tanaman Kelapa dan Palma Lain, Manado.<br />Delapan aksesi kelapa Genjah dianalisis keragamannya berdasarkan<br />penanda DNA SSRs menggunakan 3 primer atau lokus. Hasil analisis<br />menunjukkan bahwa 3 primer SSRs yang digunakan semuanya polimorfik<br />dan menghasilkan 4 - 5 alel per lokus. Kelapa Genjah Salak (GSK)<br />menunjukkan heterozigous pada ke tiga lokus, sedangkan Genjah Hijau<br />Jombang (GHJ) pada lokus CNZ 51. Dua pohon sampel dari masing-<br />masing aksesi yang dianalisis memiliki kemiripan genetik sebesar 100%<br />kecuali aksesi (GHJ) yang hanya memiliki kemiripan 93 %. Aksesi GSK<br />yang sangat berbeda dengan aksesi lainnya dengan kemiripan hanya 45%.<br />Hal ini menunjukkan bahwa aksesi kelapa GSK dan GHJ memiliki<br />beberapa karakter yang berbeda dan spesifik dibandingkan dengan aksesi<br />lainnya, sehingga diperlukan seleksi yang ketat sebelum digunakan<br />sebagai materi pemuliaan<br />Kata kunci : Kelapa,Cocos nucifera L.,plasma nutfah, keragaman genetik,<br />kelapa Genjah, penanda DNA SSRs, Sulawesi Utara<br />ABSTRACT<br />Genetic diversity evaluation of dwarf coconut germplasm<br />at Mapanget Experimental Garden based on SSRs<br />(Simple Sequence Repeats) marker<br />Genetic diversity is very important in coconut breeding program.<br />The aim of this research was to study genetic diversity of Dwarf Coconut<br />that have been planted on Mapanget Experimental Garden, Indonesian<br />Coconut and Palmae Research Institute Manado. Eight Dwarf accessions<br />were analyzed their to find out genetic diversity based on SSRs marker<br />using three primers or locus. The results showed that three primers SSRs<br />were polymophic and gave 4 -5 allels per locus. Salak Green Dwarf (SGD)<br />showed heterozygous in three loci, while Jombang Green Dwarf (JGD) in<br />locus CNZ 51. Two sample plants of each accession had genetic similarity<br />100% except JGD only had similarity of 93%. SGD was different with<br />other accessions and only had genetic similarity of 45%. SGD and JGD<br />had several and specific characters that were different compared to the<br />other accessions and have to be selected before they are used as breeding<br />materials.<br />Key words : Coconut, Cocos nucifera L., germplasm, genetic diversity<br />dwarf coconut, marker SSRs

scholarly journals Detection of Genetic Polymorphism in Seven Barley Hordeum vulgare L. Varieties Using SSR

2020 ◽  
Vol 33 (2) ◽  
pp. 115-124
Author(s):  
Maha R. Khalil ◽  
Hussain A. Almahasneh ◽  
Salam Y. Lawand
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Genetic Polymorphism ◽  
Hordeum Vulgare ◽  
Simple Sequence Repeats ◽  
Genetic Similarity ◽  
Hordeum Vulgare L ◽  
Simple Sequence

Investigation was carried out at the laboratory of Biotechnology, Faculty of Agriculture, Damascus University, during the season 2017-2018. Seven varieties were planted to determine the degree of genetic similarity using SSR-technique (Simple Sequence Repeats), and 14 double primers were used for this purpose. The analysis results revealed that all primers showed polymorphism among the evaluated varieties, except Bmag0385. primers produced a total of 42 alleles with a polymorphic percentage of 88.27%. The number of alleles for each primer varied from 1 allele for the primer (Bmac0067) to 7 alleles for the primer (Bmag0006) in average of 3 alleles per primer. Cluster analysis and Dendrogram showed the highest degree of genetic similarity between variety Arabi asuad  and variety Arabi abiad (0.7619). While it was low between variety Fourat4 and variety Arabi abiad (0.3571), and varieties Fourat4 and Fourat3 (0.3571) which indicated wide genetic diversity among them.

scholarly journals KERAGAMAN GENETIK 22 AKSESI PADI LOKAL TORAJA UTARA BERBASIS MARKA SIMPLE SEQUENCE REPEATS (SSR)

2019 ◽  
Vol 5 (2) ◽  
pp. 230
Author(s):  
Holy Ekklesia Ladjao ◽  
Rinaldi Sjahril ◽  
Muh. Riadi
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Genetic Similarity ◽  
Rice Accession ◽  
Polymorphism Rate ◽  
Simple Sequence

Genetic Diversity of 22 Local Rice Accessions from North Toraja Based on Simple Sequence Repeats (SSR) MarkersABSTRACTOne way to explore the potential of local rice is by the characterization that could obtain genetic diversity of that plants. The aim of this study was to obtain the genetic diversity of 22 local rice accession from North Toraja. Twenty-two of local rice accessions from North Toraja were characterized by 30 SSR markers and using NTSYS pc 2.1 program to analyze genetic diversity. The results showed that twenty-six SSR markers that had been analyzed produced some alelles with a size between 106.75-311 bp, the average number of alleles were 3 and the polymorphism rate was 0.53. On coefficient genetic similarity at 0.38, the population formed three clusters. Cluster I and II were dominated by rice that had no hair on the tip of the grain and cluster III were dominated by rice that had hair on the tip of the grain. There were 105 opportunities to crossing between accessions when the genetic distance was above 0.7.Keywords: genetic diversity, local rice, North Toraja, polymorphism rate, SSR markers ABSTRAKSalah satu cara untuk menggali potensi padi lokal adalah dengan karakterisasi. Dengan adanya kegiatan karakterisasi tersebut maka dapat diketahui bagaimana keragaman genetik dari suatu tanaman. Penelitian ini bertujuan untuk mengetahui keragaman genetik dari 22 aksesi padi lokal Toraja Utara. Duapuluh dua aksesi padi lokal Toraja Utara dikarakterisasi menggunakan 30 marka SSR dan dianalisis keragaman genetiknya menggunakan program NTSYS pc 2.1. Hasil penelitian menunjukkan bahwa duapuluh enam marka SSR yang dianalisis memiliki kisaran ukuran alel antara 106.75-311 bp, dengan jumlah alel rata-rata 3 dan tingkat polimorfisme sebesar 0,53. Koefisien kemiripan genetik 0,38 dan terbentuk 3 klaster. Pada klaster I dan klaster II didominasi oleh padi yang tidak memiliki rambut pada ujung gabahnya, dan pada klaster III didominasi oleh padi yang memiliki rambut pada ujung gabahnya. Selain itu, pada jarak genetik diatas 0,7 terdapat 105 peluang persilangan.Kata Kunci: keragaman genetik, marka SSR, padi lokal, tingkat polimorfisme, Toraja Utara

scholarly journals Evaluation of the Genetic Diversity and Differentiation of Black Locust (Robinia pseudoacacia L.) Based on Genomic and Expressed Sequence Tag-Simple Sequence Repeats

2018 ◽  
Vol 19 (9) ◽  
pp. 2492 ◽  
Author(s):  
Qi Guo ◽  
Xiuyu Li ◽  
Shuhong Yang ◽  
Zhiheng Yang ◽  
Yuhan Sun ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeats ◽  
Expressed Sequence Tag ◽  
Black Locust ◽  
Robinia Pseudoacacia ◽  
Ex Situ ◽  
Ssr Primers ◽  
Robinia Pseudoacacia L ◽  
Expressed Sequence ◽  
Simple Sequence

Understanding the genetic diversity and differentiation of the genetic resources of a species is important for the effective use and protection of forest tree resources. Ex situ development is a common method for the protection of genetic diversity and an essential resource for users who require ready access to a species’ germplasm. In this study, we collected seeds of black locust (Robinia pseudoacacia L.) from 19 provenances, covering most of its natural distribution; we randomly selected 367 tender leaves with well-grown and different maternal strains from this group for further analysis. Forty-eight simple sequence repeat (SSR) primers were successfully selected from 91 pairs of SSR primers using native-deformation polyacrylamide gel electrophoresis. In addition, we identified identical genotypes among all individuals and evaluated the quality of the markers. From this, 35 loci were confirmed for analyses of genetic diversity and differentiation of the black locust provenances, which contained 28 expressed sequence tag-derived simple sequence repeats (EST-SSRs) and 7 genomic DNA-derived simple sequence repeats (G-SSRs). We observed high genetic diversity among the native black locust provenances, from which Wright’s fixation index and molecular variance suggested that a majority of the genetic differentiation variation could be attributed to within-provenance differences. The genetic distance and identity results indicated that geographic distance was not a dominating factor influencing the distribution of black locust. This is the first study to evaluate provenance genetic variation in native black locust samples using two types of SSR markers, which provides a comprehensive theoretical basis for ex situ conservation and utilization of genetic resources, with an emphasis on breeding applications.

scholarly journals Keragaman Genetik Inbrida Jagung QPM dan Provit-A Berdasarkan Marka SSRs (Simple Sequence Repeats)

2016 ◽  
Vol 35 (2) ◽  
pp. 133
Author(s):  
Nining Nurini Andayani ◽  
M. Yasin H.G ◽  
Marcia B. Pabendon
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Simple Sequence Repeats ◽  
Genetic Similarity ◽  
Genetic Distances ◽  
Large Variability ◽  
Maize Germplasm ◽  
High Level ◽  
Biology Laboratory ◽  
Simple Sequence

Information on genetic diversity of QPM and Provit-A maize germplasm is important to support breeding program, in order to form a high yielding maize hybrid. Simple sequence repeats (SSR) have been extensively utilized as genetic markers to study the genetic diversity, cultivar identification, and gene mapping. The objectives of this research were to investigate the genetic diversity and to obtain information the genetic relationship among 20 maize accessions using 29 SSRs. The research was carried out at the Moleculer Biology Laboratory of Indonesian Cereals Research Institute (ICERI) in Maros, South Sulawesi. Twenty nine polymorphic primers that covered the 10 maize chromosomes were used to fingerprint the genotype of the lines, detecting 83 allels, with an average allel number of 3 allels per locus, ranging from 2 to 6 alleles per locus. The results indicated that polymorphism information content (PIC) ranged from 0.10 (nc133 and phi072) to 0.74 (phi064) with the average of 0.45. Genetic distance based on genetic similarity estimate ranged from 0.39 to 0.92. The high level of PIC values and wide genetic distances indicated the large variability among maize germplasm. Cluster analysis divided the 20 maize accessions into three groups. Coefficient cofenetic value (r) was 0.85 indicated a good fit based on the genetic similarity value. As many as 30 inbred heterotic recombinants were derived by incorporating 20 QPM and Provit-A with genetic distance of ≤0.65. The SSRs proved to be reliable and is practical technique for revealing the relationship among specialty maize genotypes.

Use of microsatellite DNA markers to investigate the level of genetic diversity and population genetic structure of coconut (Cocos nucifera L.)

Genome ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 15-21 ◽  
Author(s):  
L Perera ◽  
J R Russell ◽  
J Provan ◽  
W Powell
Keyword(s):  
Genetic Diversity ◽  
Dna Markers ◽  
Cocos Nucifera ◽  
Absolute Distance ◽  
New Information ◽  
Microsatellite Dna Markers ◽  
Ssr Primers ◽  
The World ◽  
Cocos Nucifera L ◽  
Simple Sequence

We have used eight pairs of simple sequence repeat (SSR) primers to analyse the genetic diversity in 130 individuals of coconut (Cocos nucifera L.) comprising 75 tall individuals and 55 dwarf individuals, representing 94 different coconut ecotypes throughout the world. A total of 51 alleles were detected, with an average of 6.4 alleles per locus. Fifty alleles were detected in tall coconuts (talls; mean alleles/locus 6.3) compared with only 26 (mean/ locus 3.3) in dwarfs, and the average diversity value in talls (0.589) was also significantly higher than that in dwarfs (0.348). Using the eight SSRs we were able to uniquely discriminate 116 of the 130 individuals. A phenetic tree based on DAD (absolute distance) values clustered individuals into five groups, each mainly composed of either talls or dwarfs. These results provide evidence in support of previous hypotheses concerning the dissemination of coconut, as well as important new information for conservation and breeding purposes. Key words: coconut, Cocos nucifera, microsatellites, SSR, genetic diversity.

Genetic Diversity and Characterization of a Core Collection of Malus Germplasm Using Simple Sequence Repeats (SSRs)

2011 ◽  
Vol 30 (4) ◽  
pp. 827-837 ◽  
Author(s):  
Sarah M. Potts ◽  
Yuepeng Han ◽  
M. Awais Khan ◽  
Mosbah M. Kushad ◽  
A. Lane Rayburn ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeats ◽  
Core Collection ◽  
Simple Sequence
Sign in / Sign up

Export Citation Format

Share Document