scholarly journals Study Cekaman Salinitas Terhadap Mutan Padi Pada Kondisi In Vitro

2018 ◽  
Vol 2 (1) ◽  
pp. 25 ◽  
Author(s):  
Rossa Yunita ◽  
Nurul Khumaida ◽  
Didy Sopandie ◽  
Ika Mariska
Keyword(s):  
Salinity Tolerance ◽  
In Vitro Selection ◽  
Selection Process ◽  
Width Ratio ◽  
Tolerance Mechanism ◽  
Stomata Density ◽  
Tolerant Plants ◽  
Effective Selection ◽  
Selection Of

In vitro selection using of salt (NaCl) has been used to select plants that tolerate salinity, so that effective selection process. The purpose of this study was to obtain information about the mechanism of salinity tolerance in rice in vitro conditions. This study consisted of two experiments, they are the selection of salinity tolerance somaclone putative mutants in vitro and study of salinity tolerance mechanism results in in vitro selection. The tolerant plants after being treated NaCl show response to the accumulation of proline is more, the content of K, Mg and Ca which tends to remain and Na content is lower, stomata density is fewer and the length and width ratio stomata is greater.

scholarly journals Rapid in vitro selection of salt-tolerant genotypes of the potentially medicinal plant Centaurium maritimum (L.) fritsch

2009 ◽  
Vol 61 (1) ◽  
pp. 57-69 ◽  
Author(s):  
Danijela Misic ◽  
B. Siler ◽  
Biljana Filipovic ◽  
Zorica Popovic ◽  
Suzana Zivkovic ◽  
...  
Keyword(s):  
Seed Germination ◽  
Medicinal Plant ◽  
Salinity Tolerance ◽  
Growth Phase ◽  
In Vitro Selection ◽  
Growth Phases ◽  
Salt Tolerant ◽  
Variable Response ◽  
Selection Of

We investigated differences of salinity tolerance between 'salt-tolerant' (ST) and 'salt-sensitive' (SS) genotypes of yellow centaury [Centaurium maritimum (L.) Fritsch] selected during the germination phase. The ability of in vitro cultured C. maritimum to complete the whole ontogenetic cycle in less than 6 months enabled us to deterine salinity tolerance during different growth phases. Based on the physiological attributes measured in this study (growth, morphogenesis, photosynthesis, flowering, seed germination), it can be concluded that C. maritimum genotypes differing in salinity tolerance showed a variable response to elevated salt concentrations during both the vegetative and the generative growth phase.

The characterization of herbicide tolerant plants in Brassica napus L. after in vitro selection of microspores and protoplasts

1988 ◽  
Vol 7 (2) ◽  
pp. 83-87 ◽  
Author(s):  
Eric B. Swanson ◽  
Marc P. Coumans ◽  
Gerry L. Brown ◽  
Jayanti D. Patel ◽  
W. D. Beversdorf
Keyword(s):  
Brassica Napus ◽  
In Vitro Selection ◽  
Brassica Napus L ◽  
Tolerant Plants ◽  
Herbicide Tolerant ◽  
Selection Of

scholarly journals Riboswitch identification using Ligase-Assisted Selection for the Enrichment of Responsive Ribozymes (LigASERR)

2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Matthew C Haines ◽  
Marko Storch ◽  
Diego A Oyarzún ◽  
Guy-Bart Stan ◽  
Geoff S Baldwin
Keyword(s):  
In Vitro Selection ◽  
Selection Process ◽  
Alternative Methods ◽  
Target Sequence ◽  
Selection Methods ◽  
Generating Functional ◽  
Selection For ◽  
Selection Of

Abstract In vitro selection of ligand-responsive ribozymes can identify rare, functional sequences from large libraries. While powerful, key caveats of this approach include lengthy and demanding experimental workflows; unpredictable experimental outcomes and unknown functionality of enriched sequences in vivo. To address the first of these limitations, we developed Ligase-Assisted Selection for the Enrichment of Responsive Ribozymes (LigASERR). LigASERR is scalable, amenable to automation and requires less time to implement compared to alternative methods. To improve the predictability of experiments, we modeled the underlying selection process, predicting experimental outcomes based on sequence and population parameters. We applied this new methodology and model to the enrichment of a known, in vitro-selected sequence from a bespoke library. Prior to implementing selection, conditions were optimized and target sequence dynamics accurately predicted for the majority of the experiment. In addition to enriching the target sequence, we identified two new, theophylline-activated ribozymes. Notably, all three sequences yielded riboswitches functional in Escherichia coli, suggesting LigASERR and similar in vitro selection methods can be utilized for generating functional riboswitches in this organism.

The Effects of Shock Vancomycin Concentrations on the Formation of Heteroresistance in Staphylococcus aureus

2020 ◽  
Vol 65 (9-10) ◽  
pp. 3-7
Author(s):  
V. V. Gostev ◽  
Yu. V. Sopova ◽  
O. S. Kalinogorskaya ◽  
M. E. Velizhanina ◽  
I. V. Lazareva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
In Vitro Selection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
High Concentration ◽  
Short Term ◽  
High Concentrations ◽  
Selection Of ◽  
Selection Of Resistance ◽  
Test Cultures

Glycopeptides are the basis of the treatment of infections caused by MRSA (Methicillin-Resistant Staphylococcus aureus). Previously, it was demonstrated that antibiotic tolerant phenotypes are formed during selection of resistance under the influence of high concentrations of antibiotics. The present study uses a similar in vitro selection model with vancomycin. Clinical isolates of MRSA belonging to genetic lines ST8 and ST239, as well as the MSSA (ATCC29213) strain, were included in the experiment. Test isolates were incubated for five hours in a medium with a high concentration of vancomycin (50 μg/ml). Test cultures were grown on the medium without antibiotic for 18 hours after each exposure. A total of ten exposure cycles were performed. Vancomycin was characterized by bacteriostatic action; the proportion of surviving cells after exposure was 70–100%. After selection, there was a slight increase in the MIC to vancomycin (MIC 2 μg/ml), teicoplanin (MIC 1.5–3 μg/ml) and daptomycin (MIC 0.25–2 μg/ml). According to the results of PAP analysis, all strains showed an increase in the area under curve depending on the concentration of vancomycin after selection, while a heteroresistant phenotype (with PAP/AUC 0.9) was detected in three isolates. All isolates showed walK mutations (T188S, D235N, E261V, V380I, and G223D). Exposure to short-term shock concentrations of vancomycin promotes the formation of heteroresistance in both MRSA and MSSA. Formation of VISA phenotypes is possible during therapy with vancomycin.

Wheat Germ Cell-Free Translation System as a Tool for In vitro Selection of Functional Proteins

2002 ◽  
Vol 5 (6) ◽  
pp. 473-480
Author(s):  
Bentham Science Publisher A.N. Alexandrov ◽  
Bentham Science Publisher V.Yu. Alakhov ◽  
Bentham Science Publisher A.I. Miroshnikov
Keyword(s):  
Germ Cell ◽  
Wheat Germ ◽  
In Vitro Selection ◽  
Translation System ◽  
Free Translation ◽  
Selection Of

In vitro Selection of Ligase Ribozymes Containing 2'-Amino Groups

2000 ◽  
Vol 15 (4) ◽  
pp. 297-308 ◽  
Author(s):  
NAOZUMI TERAMOTO ◽  
YUKIO IMANISHI ◽  
YOSHIHIRO ITO
Keyword(s):  
In Vitro Selection ◽  
Amino Groups ◽  
Selection Of
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