The characterization of herbicide tolerant plants in Brassica napus L. after in vitro selection of microspores and protoplasts

1988 ◽  
Vol 7 (2) ◽  
pp. 83-87 ◽  
Author(s):  
Eric B. Swanson ◽  
Marc P. Coumans ◽  
Gerry L. Brown ◽  
Jayanti D. Patel ◽  
W. D. Beversdorf
Keyword(s):  
Brassica Napus ◽  
In Vitro Selection ◽  
Brassica Napus L ◽  
Tolerant Plants ◽  
Herbicide Tolerant ◽  
Selection Of

scholarly journals Early in vitro selection of winter oilseed rape (Brassica napus L.) plants with the fertility restorer gene for CMS Shaan 2A via non-destructive molecular analysis of microspore-derived embryos

2019 ◽  
Vol 55 (No. 4) ◽  
pp. 162-165
Author(s):  
Miroslav Klíma ◽  
Eva Jozová ◽  
Irena Jelínková ◽  
Vratislav Kučera ◽  
Shengwu Hu ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
In Vitro Selection ◽  
Restorer Gene ◽  
Brassica Napus L ◽  
Rf Gene ◽  
Dh Lines ◽  
Non Destructive ◽  
Selection Of

Cotyledons of 201 microspore-derived oilseed rape (Brassica napus) embryos were analysed for the presence of the cytoplasmic male sterility Shaan 2A restorer gene using the primer pair 3F/4R. In total, 172 plants regenerated and were grown to the generative stage. Among phenotypically non-haploid flowering plants, there was a broad spectrum of different flower types from normally developed flowers to flowers with short anthers, stamens, or deformed flowers with atypically developed floral organs, often with traces of pollen. For this reason, only 115 typically fertile or sterile accessions were selected for further phenotype-genotype comparisons. In 14 plants (12.2%) the phenotype did not match the genotype: two plants were marked as fertile, but without the Rf gene. Vice versa, in 12 sterile plants the Rf gene was determined. Nevertheless, the two fertile plants did not produce any pods after self-pollination. In total, 47 doubled haploid (DH) lines with good seed yield, carrying the Rf gene were harvested.  

scholarly journals Establishment of glyphosate selective concentrations for winter rapeseed (Brassica napus L.) transgenic tissues efficient selection in vitro

2019 ◽  
Vol 25 ◽  
pp. 197-201
Author(s):  
I. S. Hnatiuk ◽  
O. I. Varchenko ◽  
M. F. Parii ◽  
Yu. V. Symonenko
Keyword(s):  
Brassica Napus ◽  
Nutrient Medium ◽  
In Vitro Selection ◽  
Brassica Napus L ◽  
Regeneration Stage ◽  
Winter Rapeseed ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Efficient Selection ◽  
Selection Of

Aim. To establish the optimal concentration of glyphosate for efficient selection of transgenic tissue culture in biotechnology of rapeseed by in vitro selection of glyphosate-resistant and non-resistant winter rape. Methods. As an explants 7–10 mm fragments of 6-day rapeseed hypocotyls were used to cultivate on the medium, supplemented with 1 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D) for 12 days at 24°C under dark conditions for the initiation of callusogenesis. For regeneration of plants, the MS nutrient medium was supplemented with 3 mg/L of 6-benzylaminopurine (BAP), 2 mg/L of zeatin, 0.1, 0.5 and 1 mM of glyphosate, respectively. As a control, glyphosate-free regenerative nutrient medium was used. The results were statistically processed using Microsoft Excel. Results. It has been shown that glyphosate-susceptible rape explants form leaves and shoots with a frequency up to 8.3 % ± 0.8 % on the medium supplemented with 0.1 mM glyphosate compared to 57.2 % ± 0.6 % in the control. However, the morphogenic structures did not pass selection after transferring shoots to the elongation medium supplemented with 0.1 mM glyphosate. Conclusions. For the in vitro selection of rapeseed 0.1 mM glyphosate can be used in a nutrient medium at the stage of adventitious buds formation. But at the regeneration stage, the amount of herbicide should be increased to avoid false-positive results. Keywords: winter rapeseed, Brassica napus L., in vitro culture, selective marker, herbicide, glyphosate.

scholarly journals Non-destructive in vitro selection of microspore-derived embryos with the fertility restorer gene for CMS Ogu-INRA in winter oilseed rape (Brassica napus L.)

2015 ◽  
Vol 18 (1) ◽  
pp. 58-60 ◽  
Author(s):  
Lenka Havlíčková ◽  
Miroslav Klíma ◽  
Marie Přibylová ◽  
Alois Antonín Hilgert-Delgado ◽  
Vratislav Kučera ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
In Vitro Selection ◽  
Winter Oilseed Rape ◽  
Fertility Restorer ◽  
Brassica Napus L ◽  
Fertility Restorer Gene ◽  
Non Destructive ◽  
Selection Of

In vitro selection of herbicide resistance in microspore-derived embryos of oilseed rape (Brassica napus L.)

2006 ◽  
Vol 3 (1) ◽  
pp. 33-38
Author(s):  
Xu Ling ◽  
Zhang Guo-Qing ◽  
Gu Hong-Hui ◽  
He Yun ◽  
Zhou Wei-Jun
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
In Vitro Selection ◽  
Chromosome Doubling ◽  
Microspore Culture ◽  
Brassica Napus L ◽  
Regenerated Plants ◽  
Short Time ◽  
Selection Of

AbstractUsing four oilseed rape (Brassica napus L.) F1 hybrids (7039, 7040, 282 and 5102) as donor plants for microspore culture, the experiment was conducted to select glyphosate- and haloxyfop-resistant embryos through application of these substances to the cultural media with microspore-derived embryos in vitro. Genotypes 7039 and 7040 were used to select glyphosate-resistant regenerated plants, and genotypes 282 and 5102 to select haloxyfop-resistant plants. The embryos at cotyledonary stage were grown on glyphosate- and haloxyfop-containing MS-2 medium for 2 weeks. The non-resistant embryos collapsed after a short time, while the resistant ones turned green and survived for 2 weeks. Transferred into the normal MS-2 medium for further plant regeneration, the regenerated plants from green embryos showed tolerance to 0.25% sprayed glyphosate, indicating the effectiveness and reliability of this in vitro selection method. When the regenerated plants selected from 0.02% haloxyfop were sprayed with 0.05% haloxyfop, most of them grew well; however, the survival rate of the regenerated plants from 0.01% haloxyfop-containing medium was lower. The present experiment indicates that the use of 0.02% haloxyfop in the selection of haloxyfop-resistant plants was more promising than that of 0.01%. The chromosome doubling efficiency of regenerated plants reached 34% and 52% after being treated with 170 mg/l colchicine for 20 and 30 h, respectively.

scholarly journals In vitro selection and extended culture of antigen-specific T lymphocytes. I. Description of selection culture procedure and initial characterization of selected cells.

1975 ◽  
Vol 142 (1) ◽  
pp. 90-105 ◽  
Author(s):  
S Z Ben-Sasson ◽  
W E Paul ◽  
E M Shevach ◽  
I Green
Keyword(s):  
T Lymphocytes ◽  
In Vitro Selection ◽  
Peritoneal Exudate ◽  
Independent State ◽  
Initial Characterization ◽  
Original Donor ◽  
Selection Of ◽  
Culture Procedure

Specific selection of antigen-responsive guinea pig peritoneal exudate lymphocytes (PELs) was achieved by a selection culture procedure. This procedure involved the addition of PELs from immune donors to monolayers of antigen-pulsed adherent peritoneal exudate cells from nonprimed syngeneic donors. PELs which failed to adhere were discarded at 24 and 48 h; after 1 wk of culture, lymphocytes were obtained which were highly responsive to the antigen for which they were selected but which demonstrated little or no response to other antigens to which the original donor of the lymphocyte was immune. These selected cells were largely T lymphocytes and could be maintained in culture for 2-5 wk in an antigen-responsive state and, in 20-30% of cases, for 8-10 wk in an antigen-independent state.

scholarly journals Study Cekaman Salinitas Terhadap Mutan Padi Pada Kondisi In Vitro

2018 ◽  
Vol 2 (1) ◽  
pp. 25 ◽  
Author(s):  
Rossa Yunita ◽  
Nurul Khumaida ◽  
Didy Sopandie ◽  
Ika Mariska
Keyword(s):  
Salinity Tolerance ◽  
In Vitro Selection ◽  
Selection Process ◽  
Width Ratio ◽  
Tolerance Mechanism ◽  
Stomata Density ◽  
Tolerant Plants ◽  
Effective Selection ◽  
Selection Of

In vitro selection using of salt (NaCl) has been used to select plants that tolerate salinity, so that effective selection process. The purpose of this study was to obtain information about the mechanism of salinity tolerance in rice in vitro conditions. This study consisted of two experiments, they are the selection of salinity tolerance somaclone putative mutants in vitro and study of salinity tolerance mechanism results in in vitro selection. The tolerant plants after being treated NaCl show response to the accumulation of proline is more, the content of K, Mg and Ca which tends to remain and Na content is lower, stomata density is fewer and the length and width ratio stomata is greater.

scholarly journals In vitro selection of l-DNA aptamers that bind a structured d-RNA molecule

2020 ◽  
Vol 48 (4) ◽  
pp. 1669-1680 ◽  
Author(s):  
Sougata Dey ◽  
Jonathan T Sczepanski
Keyword(s):  
In Vitro Selection ◽  
Rna Binding ◽  
Rna Aptamers ◽  
Dna Aptamers ◽  
Binding Properties ◽  
Rna Molecules ◽  
Dna And Rna ◽  
Selection Of

Abstract The development of structure-specific RNA binding reagents remains a central challenge in RNA biochemistry and drug discovery. Previously, we showed in vitro selection techniques could be used to evolve l-RNA aptamers that bind tightly to structured d-RNAs. However, whether similar RNA-binding properties can be achieved using aptamers composed of l-DNA, which has several practical advantages compared to l-RNA, remains unknown. Here, we report the discovery and characterization of the first l-DNA aptamers against a structured RNA molecule, precursor microRNA-155, thereby establishing the capacity of DNA and RNA molecules of the opposite handedness to form tight and specific ‘cross-chiral’ interactions with each other. l-DNA aptamers bind pre-miR-155 with low nanomolar affinity and high selectivity despite the inability of l-DNA to interact with native d-RNA via Watson–Crick base pairing. Furthermore, l-DNA aptamers inhibit Dicer-mediated processing of pre-miRNA-155. The sequence and structure of l-DNA aptamers are distinct from previously reported l-RNA aptamers against pre-miR-155, indicating that l-DNA and l-RNA interact with the same RNA sequence through unique modes of recognition. Overall, this work demonstrates that l-DNA may be pursued as an alternative to l-RNA for the generation of RNA-binding aptamers, providing a robust and practical approach for targeting structured RNAs.

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