scholarly journals Rapid in vitro selection of salt-tolerant genotypes of the potentially medicinal plant Centaurium maritimum (L.) fritsch

2009 ◽  
Vol 61 (1) ◽  
pp. 57-69 ◽  
Author(s):  
Danijela Misic ◽  
B. Siler ◽  
Biljana Filipovic ◽  
Zorica Popovic ◽  
Suzana Zivkovic ◽  
...  

We investigated differences of salinity tolerance between 'salt-tolerant' (ST) and 'salt-sensitive' (SS) genotypes of yellow centaury [Centaurium maritimum (L.) Fritsch] selected during the germination phase. The ability of in vitro cultured C. maritimum to complete the whole ontogenetic cycle in less than 6 months enabled us to deterine salinity tolerance during different growth phases. Based on the physiological attributes measured in this study (growth, morphogenesis, photosynthesis, flowering, seed germination), it can be concluded that C. maritimum genotypes differing in salinity tolerance showed a variable response to elevated salt concentrations during both the vegetative and the generative growth phase.

2003 ◽  
Vol 3 (1) ◽  
pp. 49-52 ◽  
Author(s):  
MALEE NANAKORN ◽  
WALAIKARN JIAMJETJAROON ◽  
SRISOM SUWANAWONG ◽  
CHALERMCHAI WONGWATTANA ◽  
IE SUNG SHIM

2007 ◽  
Vol 51 (4) ◽  
pp. 728-734 ◽  
Author(s):  
F. Queiros ◽  
F. Fidalgo ◽  
I. Santos ◽  
R. Salema

2011 ◽  
pp. 177-181
Author(s):  
A. Campanelli ◽  
C. Ruta ◽  
N. Calabrese ◽  
I. Morone-Fortunato

2008 ◽  
pp. 217-223 ◽  
Author(s):  
J. Troncoso ◽  
J. Liñán ◽  
M. Cantos ◽  
J.L. García ◽  
A. Troncoso

2018 ◽  
Vol 2 (1) ◽  
pp. 25 ◽  
Author(s):  
Rossa Yunita ◽  
Nurul Khumaida ◽  
Didy Sopandie ◽  
Ika Mariska

In vitro selection using of salt (NaCl) has been used to select plants that tolerate salinity, so that effective selection process. The purpose of this study was to obtain information about the mechanism of salinity tolerance in rice in vitro conditions. This study consisted of two experiments, they are the selection of salinity tolerance somaclone putative mutants in vitro and study of salinity tolerance mechanism results in in vitro selection. The tolerant plants after being treated NaCl show response to the accumulation of proline is more, the content of K, Mg and Ca which tends to remain and Na content is lower, stomata density is fewer and the length and width ratio stomata is greater.


2021 ◽  
Vol 875 (1) ◽  
pp. 012082
Author(s):  
O S Mashkina ◽  
T M Tabatskaya ◽  
O M Korchagin

Abstract In vitro modelling of stress is one of the promising avenues for plant breeding for tolerance to negative environmental factors. In this study we examined the effect of NaCl (0.5%) on callusogenesis and morphogenesis of stem explants of different birch genotypes: Betula pendula Roth, B. pendula Roth var. carelica (Mercklin) Hämet-Ahti, B. pendula f. ‘dalecarlica’ (L.f.) Schneid., B. pubescens Ehrh. In our experiments we used pre-selected microclones from our in vitro collection on NaCl (0.2-1.0%) selective media. The clones were contrasted by the degree of their sensitivity to salinity (so-called ‘stable’ and ‘sensitive’ microclones). With the use of stem callus cultures we identified informative, simple and reproducible indicators for the selection of salt-tolerant genotypes. Among these indicators were the frequency of callus formation and the viability of callus cultures, which were significantly higher in ‘stable’ group of microclones. Polyploid birch clones (2n=4x=56, 2n=3x=42) were more resistant to salination compared to diploid clones (2n=28). Our study has shown that the selection of salt-tolerant birch lines can be based on the plants’ genetic diversity presented in the collection (various species, varieties, hybrids, polyploids) and manifested in the process of in vitro cultivation, as well as in the cellular heterogeneity of callus cultures.


Sign in / Sign up

Export Citation Format

Share Document